Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,750,081 C→A 6.8% intergenic (+21/+34) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,750,0810CA 6.8% 349.5 / 9.7 122intergenic (+21/+34)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base C (55/58);  new base A (4/5);  total (59/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCA  >  NC_000913/2749934‑2750230
                                                                                                                                                   |                                                                                                                                                     
ccatttCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGAAt                                                                                                                                                     >  2:1393208/6‑150 (MQ=255)
    gTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGAAATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAg                                                                                                                                                 <  2:1990524/150‑1 (MQ=255)
     tCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAgg                                                                                                                                                <  2:903457/150‑1 (MQ=255)
        cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGATGCGCGAGAGTGTGGCGGAGTAACGAAAACTGCCCGGCATTCGCATGCAGGGcc                                                                                                                                             >  1:1104758/1‑150 (MQ=255)
        cAGGCACCCGCGTGCGTATGGGCGCGGACGATATCGATATTCTCGACGTACAGGACACTATGATTAAGCAGGTAAAAGGGTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGcc                                                                                                                                             <  1:287112/150‑1 (MQ=255)
         aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGCCg                                                                                                                                            <  2:1208128/150‑1 (MQ=255)
         aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCg                                                                                                                                            <  1:1101055/150‑1 (MQ=255)
          ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGt                                                                                                                                           >  1:534874/1‑150 (MQ=255)
            cACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGa                                                                                                                                         <  1:438780/150‑1 (MQ=255)
              ccggcgtgcgtATTGGCGATTACGATATCGATATTCACGACGAACAGGACAATATGATTAAGCAGGTAAAAGTTTTACCTGGGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCAGCATGCGAATGCCGGGCCGttttc                                                                                                                                       <  2:850759/147‑5 (MQ=255)
              cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGCCGttttc                                                                                                                                       <  1:350401/150‑5 (MQ=255)
              cccGCGTGCGTATTGGCGAGTACGATATCGAAAATCTCGACGTACAGGACAATATGAGTAAGCAGCTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGACGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAat                                                                                                                                       <  2:885318/150‑1 (MQ=255)
               ccGCGTGCGTATTGGCGAGTCCGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTTTGAAACCGCTGCGCGAGAGTGTGTCGGAGTAACGACAACGGCCCGGCATTCGCATGCAGGGCCGTGAatt                                                                                                                                      <  1:119847/150‑1 (MQ=255)
                cgcgTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGCCGttttcgt                                                                                                                                     <  2:41936/150‑7 (MQ=255)
                   gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAAttatta                                                                                                                                  <  1:641038/150‑1 (MQ=255)
                    tgcgtATTGTCGAGTACGTTATCGATATTCTCGACGTACAGGACAATATGATTAAGCATGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGAATGCAGGGCCGTGAATTATTAc                                                                                                                                 <  2:184187/150‑1 (MQ=255)
                    tgagtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTAc                                                                                                                                 <  1:425368/147‑1 (MQ=255)
                      cgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACTGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGa                                                                                                                               <  1:1604801/150‑1 (MQ=255)
                      cgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGa                                                                                                                               <  2:240041/150‑1 (MQ=255)
                        tATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGaaa                                                                                                                             >  2:191029/1‑150 (MQ=255)
                        tATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGACGTGAATTATTACGaaa                                                                                                                             >  1:1652768/1‑150 (MQ=255)
                         aTTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAg                                                                                                                            >  1:1370375/1‑150 (MQ=255)
                          ttGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGc                                                                                                                           <  1:1557411/150‑1 (MQ=255)
                            ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                         >  1:1898038/1‑150 (MQ=255)
                            ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                         <  2:1243107/150‑1 (MQ=255)
                            ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                         >  2:1426280/1‑150 (MQ=255)
                            ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                         >  1:1681458/1‑150 (MQ=255)
                                      atatagatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCTCTCCGCGAGAGTGTGGCTTAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                                               <  1:369686/150‑1 (MQ=255)
                                       tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCtt                                                                                                              >  2:1612286/1‑150 (MQ=255)
                                       tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTACTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGCGCCGTGAATTATTACGAAAGCAGAAATTAAGCtt                                                                                                              >  2:1747882/1‑150 (MQ=255)
                                          cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                           <  2:265679/150‑1 (MQ=255)
                                           gatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGc                                                                                                          >  2:153821/1‑150 (MQ=255)
                                            atatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                         >  2:1357504/1‑150 (MQ=255)
                                              atTCTCGACGTACAGGACTATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                       <  1:930793/150‑1 (MQ=255)
                                                 ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGTCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                    >  2:590539/1‑150 (MQ=255)
                                                 ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                    >  2:897156/1‑150 (MQ=255)
                                                 ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                    <  2:1512577/150‑1 (MQ=255)
                                                 ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                    <  2:1504302/150‑1 (MQ=255)
                                                 ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                    <  1:1943055/150‑1 (MQ=255)
                                                  tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCt                                                                                                   <  1:1471841/150‑1 (MQ=255)
                                                      cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                               >  2:659165/1‑150 (MQ=255)
                                                       gTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGt                                                                                              >  2:590044/1‑150 (MQ=255)
                                                                 atatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACCGCCCGCCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATAAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                                    >  2:384344/1‑150 (MQ=255)
                                                                      aTTAAGCATGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                               <  2:1928581/150‑1 (MQ=255)
                                                                               gTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACg                                                                      >  2:1106291/1‑150 (MQ=255)
                                                                                tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTATCCATCGCCGCACGAATCGTACgc                                                                     >  2:1037423/1‑149 (MQ=255)
                                                                                tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                     >  1:1132708/1‑150 (MQ=255)
                                                                                tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                     >  2:1268498/1‑150 (MQ=255)
                                                                                 aaaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGTGCCGTGATTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATAGCCGCACGAATCGTACGAc                                                                    >  2:1277940/1‑150 (MQ=255)
                                                                                   aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCa                                                                  >  2:247030/1‑150 (MQ=255)
                                                                                      tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTc                                                               <  2:194391/150‑1 (MQ=255)
                                                                                      tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTc                                                               <  2:1363626/150‑1 (MQ=255)
                                                                                           ccTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                          <  1:1543333/150‑1 (MQ=255)
                                                                                            cTGTGAAACCGGTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCTCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                         <  2:1543311/150‑1 (MQ=255)
                                                                                               tgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                      >  1:1865146/1‑150 (MQ=255)
                                                                                               tgAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                      <  1:1725488/150‑1 (MQ=255)
                                                                                                taatccGCTGCGCGAGAGTGTGGCGGTGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCCGCGTATAAc                                                     <  1:1901438/146‑1 (MQ=255)
                                                                                                 aaaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCATTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAGCATTCAGCGTATAccc                                                    >  1:533844/1‑150 (MQ=255)
                                                                                                  aaCCGCTGCGCGTTTTTGTGGCGGTTTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                                   <  2:2046645/150‑1 (MQ=255)
                                                                                                  aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCATTTGCTTTCGCTACAGTAACCATCGCCCCACGAATCGTACGAACATTCAGCGTATAAccc                                                   >  2:1756154/1‑150 (MQ=255)
                                                                                                      gCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTTTTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGCCCATTCAGCGTATAACCCTTCt                                               >  1:889114/1‑150 (MQ=255)
                                                                                                      gCTGCGCGAGAGTGTGGCGGAGTAAAGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                               <  1:876955/150‑1 (MQ=255)
                                                                                                       cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTg                                              <  2:117198/150‑1 (MQ=255)
                                                                                                         gcgcGAGAGTGTGGCGGAGTATCGAAAACGGCCCGGCAGACGCATGCAGGGGCGTGAATTAATACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCa                                            <  2:1796166/150‑1 (MQ=255)
                                                                                                         gcgcGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCa                                            <  1:1407497/150‑1 (MQ=255)
                                                                                                         gcgcGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCa                                            >  2:183292/1‑150 (MQ=255)
                                                                                                         gcgcGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCa                                            <  1:1875249/150‑1 (MQ=255)
                                                                                                          cgcgTGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                           <  2:1860529/150‑1 (MQ=255)
                                                                                                          cgcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                           <  2:1632964/150‑1 (MQ=255)
                                                                                                              agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                       <  2:1686259/150‑1 (MQ=255)
                                                                                                                 gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                                    <  1:748431/150‑1 (MQ=255)
                                                                                                                 gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                                    <  1:862293/150‑1 (MQ=255)
                                                                                                                  tgtgGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa                                   >  1:249903/1‑150 (MQ=255)
                                                                                                                   gtgGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTAGCTTTCGCTACAGTAACCATCGCAGCACGAATCGTACGGCAATTCACCGTATAACCCTTCTGCATAATGCCCAg                                  >  2:87719/1‑150 (MQ=255)
                                                                                                                     ggCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGCATTATTACGAAAGCAGCAATTAAGCTTTTGCTTTCGCTTCAGTAACCATCGCCGCACGAATCGTACGTCCAGTCCGCGTATACCCCTTCTGCATAATGCCCAGtc                                >  2:940672/1‑149 (MQ=255)
                                                                                                                     ggCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                                >  2:1619115/1‑150 (MQ=255)
                                                                                                                      gCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTGAGCGTATAACCCTTCTGCATAATGCCCAGTAc                               >  1:452319/1‑150 (MQ=255)
                                                                                                                         gAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt                            <  2:534874/150‑1 (MQ=255)
                                                                                                                           gTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGCATCGTTCGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                          >  1:1846077/1‑150 (MQ=255)
                                                                                                                           gTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                          >  1:389519/1‑150 (MQ=255)
                                                                                                                           gTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                          <  2:129245/150‑1 (MQ=255)
                                                                                                                             aaCGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                        >  1:1869066/1‑150 (MQ=255)
                                                                                                                             aaCGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCAGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAccg                        >  1:598316/1‑149 (MQ=255)
                                                                                                                              aCGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTg                       <  1:1745621/150‑1 (MQ=255)
                                                                                                                                 aaacCGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTCACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgcg                    <  2:714370/150‑1 (MQ=255)
                                                                                                                                    aCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCaa                 >  2:2023496/1‑150 (MQ=255)
                                                                                                                                     cGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAAc                >  1:1956798/1‑150 (MQ=255)
                                                                                                                                      ggCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAGCGCCGCACGAATCGTACGACCATTCATCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg               <  1:337902/150‑1 (MQ=255)
                                                                                                                                       gCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGt              >  2:571919/1‑150 (MQ=255)
                                                                                                                                       gCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTACCCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCAAGTACGTTACCTGGCGCAACGt              >  2:172891/1‑150 (MQ=255)
                                                                                                                                       gCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATACCCCTTCTGCATAATGCCCAGTACGTTACCGGGCGCAACGt              >  2:172895/1‑150 (MQ=255)
                                                                                                                                       gCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGt              >  1:805710/1‑150 (MQ=255)
                                                                                                                                         ccGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca            <  1:1397771/150‑1 (MQ=255)
                                                                                                                                           ggCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc          >  2:472204/1‑150 (MQ=255)
                                                                                                                                           ggCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc          >  2:464600/1‑150 (MQ=255)
                                                                                                                                            gCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatca         <  1:1357505/150‑1 (MQ=255)
                                                                                                                                               ttCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGGATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGAt      >  1:801705/1‑150 (MQ=255)
                                                                                                                                                tCGCATGCAGGGACGTGAATTATTACGAAAGCAGAAATTAAGCTCTTGCGTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGAtt     <  1:1251797/150‑1 (MQ=255)
                                                                                                                                                  gCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTcc   <  1:604342/150‑1 (MQ=255)
                                                                                                                                                   cATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTcca  <  2:1132707/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCA  >  NC_000913/2749934‑2750230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: