Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,750,086 A→C 5.6% intergenic (+26/+29) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,750,0860AC 5.6% 382.6 / 8.8 125intergenic (+26/+29)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base A (58/60);  new base C (3/4);  total (61/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

Only 100 of 125 total aligned reads displayed.

GTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCA  >  NC_000913/2749938‑2750233
                                                                                                                                                    |                                                                                                                                                   
gTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGAAATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAg                                                                                                                                                    <  2:1990524/150‑1 (MQ=255)
 tCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAgg                                                                                                                                                   <  2:903457/150‑1 (MQ=255)
    cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGATGCGCGAGAGTGTGGCGGAGTAACGAAAACTGCCCGGCATTCGCATGCAGGGcc                                                                                                                                                >  1:1104758/1‑150 (MQ=255)
    cAGGCACCCGCGTGCGTATGGGCGCGGACGATATCGATATTCTCGACGTACAGGACACTATGATTAAGCAGGTAAAAGGGTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGcc                                                                                                                                                <  1:287112/150‑1 (MQ=255)
     aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCg                                                                                                                                               <  1:1101055/150‑1 (MQ=255)
      ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGt                                                                                                                                              >  1:534874/1‑150 (MQ=255)
          ccggcgtgcgtATTGGCGATTACGATATCGATATTCACGACGAACAGGACAATATGATTAAGCAGGTAAAAGTTTTACCTGGGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCAGCATGCGAATGCCGGGCCGttttc                                                                                                                                          <  2:850759/147‑5 (MQ=255)
          cccGCGTGCGTATTGGCGAGTACGATATCGAAAATCTCGACGTACAGGACAATATGAGTAAGCAGCTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGACGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAat                                                                                                                                          <  2:885318/150‑1 (MQ=255)
           ccGCGTGCGTATTGGCGAGTCCGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTTTGAAACCGCTGCGCGAGAGTGTGTCGGAGTAACGACAACGGCCCGGCATTCGCATGCAGGGCCGTGAatt                                                                                                                                         <  1:119847/150‑1 (MQ=255)
            cgcgTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGCCGttttcgt                                                                                                                                        <  2:41936/150‑7 (MQ=255)
               gtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAAttatta                                                                                                                                     <  1:641038/150‑1 (MQ=255)
                tgcgtATTGTCGAGTACGTTATCGATATTCTCGACGTACAGGACAATATGATTAAGCATGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGAATGCAGGGCCGTGAATTATTAc                                                                                                                                    <  2:184187/150‑1 (MQ=255)
                tgagtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTAc                                                                                                                                    <  1:425368/147‑1 (MQ=255)
                  cgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGa                                                                                                                                  <  2:240041/150‑1 (MQ=255)
                    tATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGACGTGAATTATTACGaaa                                                                                                                                >  1:1652768/1‑150 (MQ=255)
                     aTTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAg                                                                                                                               >  1:1370375/1‑150 (MQ=255)
                      ttGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGc                                                                                                                              <  1:1557411/150‑1 (MQ=255)
                      ttGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGc                                                                                                                              >  1:385216/1‑150 (MQ=255)
                        ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                            >  2:1426280/1‑150 (MQ=255)
                        ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                            >  1:1898038/1‑150 (MQ=255)
                        ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                            <  2:1243107/150‑1 (MQ=255)
                        ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                                            >  1:1681458/1‑150 (MQ=255)
                        ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTAAGAAAGCac                                                                                                                            >  1:1778803/1‑149 (MQ=255)
                              tACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGAAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTa                                                                                                                      >  1:1352799/1‑150 (MQ=255)
                                  atatagatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCTCTCCGCGAGAGTGTGGCTTAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                                                  <  1:369686/150‑1 (MQ=255)
                                   tatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTACTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGCGCCGTGAATTATTACGAAAGCAGAAATTAAGCtt                                                                                                                 >  2:1747882/1‑150 (MQ=255)
                                      cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                              <  2:265679/150‑1 (MQ=255)
                                      cgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTg                                                                                                              >  2:1472988/1‑150 (MQ=255)
                                       gatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGc                                                                                                             >  2:153821/1‑150 (MQ=255)
                                        atatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                            <  2:1763684/150‑1 (MQ=255)
                                        atatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCt                                                                                                            >  2:1357504/1‑150 (MQ=255)
                                          atTCTCGACGTACAGGACTATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                                          <  1:930793/150‑1 (MQ=255)
                                             ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGTCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                       >  2:590539/1‑150 (MQ=255)
                                             ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                       <  2:1512577/150‑1 (MQ=255)
                                             ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                       >  2:897156/1‑150 (MQ=255)
                                             ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCGGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                                       <  1:1943055/150‑1 (MQ=255)
                                              tcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCt                                                                                                      <  1:1471841/150‑1 (MQ=255)
                                               cGACCTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                                                                                                     <  1:1611508/150‑1 (MQ=255)
                                                gACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGTAGTAACGAAAACGGCCCGACATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTAc                                                                                                    <  1:987204/150‑1 (MQ=255)
                                                  cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                                  >  2:1706640/1‑150 (MQ=255)
                                                  cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                                  >  2:659165/1‑150 (MQ=255)
                                                   gTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGt                                                                                                 >  2:590044/1‑150 (MQ=255)
                                                   gTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAAAAGCTTAATTTCTGCTTTCgtaataat                                                                                                 <  1:1440955/150‑8 (MQ=38)
                                                        ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACTGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCGACAGTAACCa                                                                                            >  1:1880015/1‑150 (MQ=255)
                                                             atatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                                       >  1:616839/1‑150 (MQ=255)
                                                             atatGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACCGCCCGCCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATAAAGCTTTTGCTTTCGCTACAGTAACCATcgcc                                                                                       >  2:384344/1‑150 (MQ=255)
                                                                  aTTAAGCATGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                                  <  2:1928581/150‑1 (MQ=255)
                                                                            tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTATCCATCGCCGCACGAATCGTACgc                                                                        >  2:1037423/1‑149 (MQ=255)
                                                                            tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                        >  1:1132708/1‑150 (MQ=255)
                                                                            tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                                        >  2:1268498/1‑150 (MQ=255)
                                                                             aaaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGTGCCGTGATTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATAGCCGCACGAATCGTACGAc                                                                       >  2:1277940/1‑150 (MQ=255)
                                                                               aaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCa                                                                     >  2:247030/1‑150 (MQ=255)
                                                                                  tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTc                                                                  <  2:194391/150‑1 (MQ=255)
                                                                                  tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTc                                                                  <  2:1363626/150‑1 (MQ=255)
                                                                                       ccTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                                             <  1:1543333/150‑1 (MQ=255)
                                                                                        cTGTGAAACCGGTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCTCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGta                                                            <  2:1543311/150‑1 (MQ=255)
                                                                                           tgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                         >  1:1865146/1‑150 (MQ=255)
                                                                                           tgAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATaa                                                         <  1:1725488/150‑1 (MQ=255)
                                                                                            taatccGCTGCGCGAGAGTGTGGCGGTGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCCGCGTATAAc                                                        <  1:1901438/146‑1 (MQ=255)
                                                                                             aaaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCATTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAGCATTCAGCGTATAccc                                                       >  1:533844/1‑150 (MQ=255)
                                                                                              aaCCGCTGCGCGTTTTTGTGGCGGTTTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAAccc                                                      <  2:2046645/150‑1 (MQ=255)
                                                                                              aaCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCATTTGCTTTCGCTACAGTAACCATCGCCCCACGAATCGTACGAACATTCAGCGTATAAccc                                                      >  2:1756154/1‑150 (MQ=255)
                                                                                                  gCTGCGCGAGAGTGTGGCGGAGTAAAGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCt                                                  <  1:876955/150‑1 (MQ=255)
                                                                                                   cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTg                                                 <  2:117198/150‑1 (MQ=255)
                                                                                                    tacgcgAGAGTGTGGCTGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGc                                                <  1:1968734/148‑1 (MQ=255)
                                                                                                     gcgcGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCa                                               >  2:183292/1‑150 (MQ=255)
                                                                                                      cgcgTGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                              <  2:1860529/150‑1 (MQ=255)
                                                                                                      cgcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCAt                                              <  2:1632964/150‑1 (MQ=255)
                                                                                                          agagTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATg                                          <  2:1686259/150‑1 (MQ=255)
                                                                                                             gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                                       <  1:748431/150‑1 (MQ=255)
                                                                                                             gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                                       <  1:862293/150‑1 (MQ=255)
                                                                                                              tgtgGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCa                                      >  1:249903/1‑150 (MQ=255)
                                                                                                               gtgGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTAGCTTTCGCTACAGTAACCATCGCAGCACGAATCGTACGGCAATTCACCGTATAACCCTTCTGCATAATGCCCAg                                     >  2:87719/1‑150 (MQ=255)
                                                                                                                 ggCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGCATTATTACGAAAGCAGCAATTAAGCTTTTGCTTTCGCTTCAGTAACCATCGCCGCACGAATCGTACGTCCAGTCCGCGTATACCCCTTCTGCATAATGCCCAGtc                                   >  2:940672/1‑149 (MQ=255)
                                                                                                                 ggCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTa                                   >  2:1619115/1‑150 (MQ=255)
                                                                                                                  gCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTGAGCGTATAACCCTTCTGCATAATGCCCAGTAc                                  >  1:452319/1‑150 (MQ=255)
                                                                                                                     gAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGtt                               <  2:534874/150‑1 (MQ=255)
                                                                                                                       gTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGCATCGTTCGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                             >  1:1846077/1‑150 (MQ=255)
                                                                                                                       gTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTAc                             >  1:389519/1‑150 (MQ=255)
                                                                                                                         aaCGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAATGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                           <  2:1558724/150‑1 (MQ=255)
                                                                                                                         aaCGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCt                           >  1:1869066/1‑150 (MQ=255)
                                                                                                                           cGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGATTTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTgg                         >  1:517307/1‑150 (MQ=255)
                                                                                                                             aaacCGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTCACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGgcg                       <  2:714370/150‑1 (MQ=255)
                                                                                                                                aCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCaa                    >  2:2023496/1‑150 (MQ=255)
                                                                                                                                  ggCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAGCGCCGCACGAATCGTACGACCATTCATCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg                  <  1:337902/150‑1 (MQ=255)
                                                                                                                                   gCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGt                 >  2:571919/1‑150 (MQ=255)
                                                                                                                                   gCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATACCCCTTCTGCATAATGCCCAGTACGTTACCGGGCGCAACGt                 >  2:172895/1‑150 (MQ=255)
                                                                                                                                   gCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGt                 >  1:805710/1‑150 (MQ=255)
                                                                                                                                    cccGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCGTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtc                >  1:82831/1‑150 (MQ=255)
                                                                                                                                     ccGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtca               <  1:1397771/150‑1 (MQ=255)
                                                                                                                                       ggCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc             >  2:472204/1‑150 (MQ=255)
                                                                                                                                       ggCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatc             >  2:464600/1‑150 (MQ=255)
                                                                                                                                        gCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGtcatca            <  1:1357505/150‑1 (MQ=255)
                                                                                                                                             cGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACAATTCAGCGTTTAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACCTCATCAGATTc       >  2:1189545/1‑150 (MQ=255)
                                                                                                                                              gCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTcc      <  1:604342/150‑1 (MQ=255)
                                                                                                                                               cATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTcca     <  2:1132707/150‑1 (MQ=255)
                                                                                                                                               cATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTcca     <  2:1898037/150‑1 (MQ=255)
                                                                                                                                                  gCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACTTTCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCGTCTGCAGAATGCCCAGTACGTTCCCTGGCGCAAAGTCCTCTGATTccacca  >  2:1265179/1‑150 (MQ=255)
                                                                                                                                                  gCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGGATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTacacca  >  1:963696/1‑150 (MQ=255)
                                                                                                                                                  gCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACAATTCAGCGTATAACCCTTCTGCATAATGCCCAGAACGTTACCTGGCGCAACGTCATCAGATTcaacca  >  2:1133886/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGTCATCAGATTCCACCA  >  NC_000913/2749938‑2750233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: