Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,757,418 C→A 6.2% intergenic (+18/+226) intA → / ← yfjH CP4‑57 prophage; integrase/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,757,4180CA 6.2% 362.6 / 8.1 125intergenic (+18/+226)intA/yfjHCP4‑57 prophage; integrase/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (58/58);  new base A (4/5);  total (62/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

GCGCTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTTG  >  NC_000913/2757270‑2757567
                                                                                                                                                    |                                                                                                                                                     
gcgcTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGct                                                                                                                                                      >  1:237516/1‑150 (MQ=255)
   cTTATAACCGCAGTGCTTATCTTGAGCAACGTCGTCCGATGATGCAATAGTGGGCTGCTCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAATATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGctctc                                                                                                                                                   >  2:1131770/1‑150 (MQ=255)
      ataACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTgg                                                                                                                                                >  2:677913/1‑150 (MQ=255)
       taACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTggg                                                                                                                                               >  2:2042152/1‑150 (MQ=255)
         aCCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCt                                                                                                                                             <  1:1050801/150‑1 (MQ=255)
         aCCGCAGTGATTATCTTGAGCAACGTCGGCCGCTGCTGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCt                                                                                                                                             <  2:134034/150‑1 (MQ=255)
            gCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGat                                                                                                                                          >  2:1938891/1‑150 (MQ=255)
            gCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGat                                                                                                                                          <  2:1605078/150‑1 (MQ=255)
            gCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGat                                                                                                                                          <  1:626333/150‑1 (MQ=255)
             cAGTGATTCTCTTGCGCAACGGCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAACGCAGCAGATAGTGGTATCATCGTTTTAACTCATTTGAGCAAAATTCGGCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGata                                                                                                                                         <  2:1275090/150‑1 (MQ=255)
               gTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATAtt                                                                                                                                       <  1:965642/150‑1 (MQ=255)
                 gATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATAttta                                                                                                                                     >  1:1583554/1‑149 (MQ=255)
                 gATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTc                                                                                                                                     <  2:586455/150‑1 (MQ=255)
                 gATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTc                                                                                                                                     >  1:1598103/1‑150 (MQ=255)
                  aTTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCt                                                                                                                                    >  2:465865/1‑150 (MQ=255)
                    tATCTTGAGCAACGTCTGCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGAGAGCTGGGCTGataaatattt                                                                                                                                  <  1:853618/150‑8 (MQ=255)
                     aTCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaaa                                                                                                                                 >  1:781768/1‑150 (MQ=255)
                     aTCTTGAGCAACGTCGTCCGATGAAGCAATTGTGGGCTGATCTCGAAAAAGCAGCAGATAGTGGAATCATCGTTTTAACTCATTTGAGCAAAATTCGACTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaaa                                                                                                                                 <  1:80161/150‑1 (MQ=255)
                      tCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTaaaa                                                                                                                                <  2:1794335/150‑1 (MQ=255)
                       cTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAg                                                                                                                               <  1:1841204/150‑1 (MQ=255)
                        ttGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGa                                                                                                                              >  2:856892/1‑150 (MQ=255)
                        ttGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGa                                                                                                                              <  2:752872/150‑1 (MQ=255)
                         tGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGAt                                                                                                                             >  1:739626/1‑150 (MQ=255)
                            gCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGc                                                                                                                          >  2:9468/1‑150 (MQ=255)
                            gCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGc                                                                                                                          <  1:1858878/150‑1 (MQ=255)
                            gCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAAAATTTATCAGCCCAGAAGTATGGGCTGATATTTCTAAAAGATccc                                                                                                                          >  2:210761/1‑148 (MQ=255)
                             cAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCa                                                                                                                         <  1:1894545/150‑1 (MQ=255)
                              aaCGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCATCAGATAGTTGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCAc                                                                                                                        <  2:1706620/150‑1 (MQ=255)
                              aaCGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCTTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCAc                                                                                                                        <  1:1435386/150‑1 (MQ=255)
                                cgtcgtCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCTTCGTTTTAACTCATTTGAGAAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTTATATTTCTAAAAGATCGCACTa                                                                                                                      <  2:1557104/150‑1 (MQ=255)
                                     tcCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAg                                                                                                                 <  1:548022/150‑1 (MQ=255)
                                      ccGATGATGCAATGGTGGGCTGATCTCGTTAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGc                                                                                                                <  2:1539185/150‑1 (MQ=255)
                                      ccGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGc                                                                                                                >  2:1281722/1‑150 (MQ=255)
                                            atgCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTCTCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTAcgcg                                                                                                          <  2:1701166/150‑1 (MQ=255)
                                             tgCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCgg                                                                                                         <  2:1206201/150‑1 (MQ=255)
                                                  tggtggGCTGATCTCTTAAAAGCAGCAGATAGTGGTAGAATCGTTTTAACTAATTTGAGCAAAATTCGTCTTTTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAg                                                                                                    <  1:1048862/150‑1 (MQ=255)
                                                  tggtggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAg                                                                                                    <  2:659424/150‑1 (MQ=255)
                                                    gtggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGATACGCGGAATATTg                                                                                                  >  1:940124/1‑150 (MQ=255)
                                                     tggGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGc                                                                                                 <  2:1403223/150‑1 (MQ=255)
                                                       ggCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGctc                                                                                               <  1:877161/150‑1 (MQ=255)
                                                              ctcGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCGGATATTTCTTAACGCTCGCACTAAATAGCTACGCGGAATAGTGGTCTACTTTc                                                                                        >  2:363068/1‑150 (MQ=255)
                                                               tcGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCg                                                                                       >  1:1970721/1‑150 (MQ=255)
                                                                 gTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGtt                                                                                     >  2:1126406/1‑150 (MQ=255)
                                                                  tAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGttt                                                                                    <  1:33225/150‑1 (MQ=255)
                                                                   aaaaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTAGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTa                                                                                   <  1:380201/150‑1 (MQ=255)
                                                                     aaGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCGGATATTTCTAAAAGAACGCACTAAATAGCTCCGCGGCATAGTGCTCTCCTTTCGTTTAGc                                                                                 >  1:992259/1‑150 (MQ=255)
                                                                     aaGCAGCAGATAGTGGTAGCATCGGTATCACTCATTAGAGCAAAATGCGTCATGTCGGATAAAATAATTCTCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGc                                                                                 <  2:1256982/150‑1 (MQ=255)
                                                                      agcagcAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGcc                                                                                >  2:1629382/1‑150 (MQ=255)
                                                                         agcagATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCtt                                                                             >  1:1356740/1‑150 (MQ=255)
                                                                             gATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATTAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCag                                                                         >  1:203312/1‑150 (MQ=255)
                                                                             gATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCag                                                                         <  2:1771896/150‑1 (MQ=255)
                                                                              ttAGTGGTATCATCGTTTTAACTCATTTGAGCAAAATTATTCTTGTCTGATAAAATATTAATCATCAAAGCTCTCTGTGCTGATATTTCTTAAAGATCGCACTAAATAGCTAAGCGTAATAGTGCTATACTTTCGTTTAGCCCTTTCaga                                                                        <  2:106350/149‑1 (MQ=255)
                                                                              aTAGTGGTAGCATCGTTTTAAATCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCGAGCTCTCTGGGCTGATATTTCTAAATGATCTCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCaga                                                                        <  1:1468809/150‑1 (MQ=255)
                                                                                aGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAgg                                                                      <  2:1153353/150‑1 (MQ=255)
                                                                                aGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCACTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAgg                                                                      <  1:146320/150‑1 (MQ=255)
                                                                                 gTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGt                                                                     >  2:1614816/1‑150 (MQ=255)
                                                                                 gTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGt                                                                     <  1:1938892/150‑1 (MQ=255)
                                                                                 gTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCACTTTCAGAGGt                                                                     >  2:349358/1‑150 (MQ=255)
                                                                                   ggTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTaa                                                                   >  2:1718168/1‑150 (MQ=255)
                                                                                    gTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAg                                                                  <  1:475230/150‑1 (MQ=255)
                                                                                     tAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGa                                                                 <  1:465865/150‑1 (MQ=255)
                                                                                        cATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACaa                                                              >  1:429827/1‑149 (MQ=255)
                                                                                             ttttAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAggg                                                         <  2:1093235/150‑1 (MQ=255)
                                                                                             ttttAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAggg                                                         <  1:1737934/150‑1 (MQ=255)
                                                                                                tAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCaa                                                      >  2:1105870/1‑150 (MQ=255)
                                                                                                 aaCTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAAt                                                     >  1:1323477/1‑150 (MQ=255)
                                                                                                  aCTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCCGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTGCAGAGGTAAGACATTAGGGCAAtt                                                    >  1:1314544/1‑150 (MQ=255)
                                                                                                   cTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAAttt                                                   <  2:188005/150‑1 (MQ=255)
                                                                                                     cATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCa                                                 >  1:33288/1‑150 (MQ=255)
                                                                                                            gCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATg                                          >  2:30304/1‑150 (MQ=255)
                                                                                                              aaaaTTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGAc                                        <  1:2051054/150‑1 (MQ=255)
                                                                                                                 aTTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTATGGCAATTTCACTGTATGACTgc                                     <  1:1174504/150‑1 (MQ=255)
                                                                                                                   tCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTgcgc                                   >  2:569773/1‑150 (MQ=255)
                                                                                                                   tCGTCTTGTCGGATAAAATATATCTCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTgcgc                                   <  1:1520653/150‑1 (MQ=255)
                                                                                                                     gTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAg                                 <  1:1780770/150‑1 (MQ=255)
                                                                                                                        ttGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCa                              >  2:1176912/1‑150 (MQ=255)
                                                                                                                        ttGTCGGATAAAATAATTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCa                              <  2:1338789/150‑1 (MQ=255)
                                                                                                                         tGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATAAGGGCAATTTCCCTGTATGACTGCGCAGTCAt                             >  2:646434/1‑150 (MQ=255)
                                                                                                                          gTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGTGCAATTACACTGTATGACTGCGCAGTCATc                            >  2:1838494/1‑150 (MQ=255)
                                                                                                                           tccgctAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCg                           <  1:210761/145‑1 (MQ=255)
                                                                                                                           tCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCg                           <  1:9468/150‑1 (MQ=255)
                                                                                                                            cGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCAACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGAGCAGTCAGCGa                          >  1:856479/1‑150 (MQ=255)
                                                                                                                             ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAg                         <  2:250921/150‑1 (MQ=255)
                                                                                                                             ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAg                         <  2:1789400/150‑1 (MQ=255)
                                                                                                                             ggATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAg                         >  1:766671/1‑150 (MQ=255)
                                                                                                                               aTAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCTGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCAtacagtg                       <  1:1281723/150‑7 (MQ=255)
                                                                                                                               aTAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTc                       <  2:953049/150‑1 (MQ=255)
                                                                                                                                tAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCt                      >  1:438555/1‑150 (MQ=255)
                                                                                                                                tAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCt                      >  2:1947384/1‑150 (MQ=255)
                                                                                                                                tAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTATACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCt                      >  2:75539/1‑150 (MQ=255)
                                                                                                                                     tatTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCTCTAAATAGCTACGCGGATTAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTATGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTggg                 <  1:490647/150‑1 (MQ=255)
                                                                                                                                      atTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCAAGTCTGTgggg                >  2:3494/1‑150 (MQ=255)
                                                                                                                                       tttATCAGCCCAGCTATCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGgt               >  2:394937/1‑150 (MQ=255)
                                                                                                                                         tATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGACTGTGGGgtgt             >  2:1499297/1‑150 (MQ=255)
                                                                                                                                          aTCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGtt            >  2:1698603/1‑150 (MQ=255)
                                                                                                                                           tCAGCCCAGAGAGCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTg           >  1:1029644/1‑150 (MQ=255)
                                                                                                                                              gCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTATGTGGGGTGTTGCTc        >  2:1234718/1‑150 (MQ=255)
                                                                                                                                               cccAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTcc       <  1:1932142/150‑1 (MQ=255)
                                                                                                                                                 cAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTTCGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATCCATGCGCAGTCATCGAGTCTGTGTGGTTTTGCTCCtt     >  2:378413/1‑150 (MQ=255)
                                                                                                                                                    ctctctGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTTg  <  2:1370594/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGCTTATAACCGCAGTGATTATCTTGAGCAACGTCGTCCGATGATGCAATGGTGGGCTGATCTCGTAAAAGCAGCAGATAGTGGTAGCATCGTTTTAACTCATTTGAGCAAAATTCGTCTTGTCGGATAAAATATTTATCAGCCCAGCTCTCTGGGCTGATATTTCTAAAAGATCGCACTAAATAGCTACGCGGAATAGTGCTCTACTTTCGTTTAGCCCTTTCAGAGGTAAGACATTAGGGCAATTTCACTGTATGACTGCGCAGTCATCGAGTCTGTGGGGTGTTGCTCCTTTTG  >  NC_000913/2757270‑2757567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: