Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,531 G→T 15.3% intergenic (+70/‑284) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,5310GT15.3% 158.1 / 11.9 71intergenic (+70/‑284)nrdF/proVribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (39/20);  new base T (2/10);  total (41/30)
Fisher's exact test for biased strand distribution p-value = 2.72e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATT  >  NC_000913/2804382‑2804680
                                                                                                                                                     |                                                                                                                                                      
aCGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACg                                                                                                                                                        <  1:421897/150‑1 (MQ=255)
         tttCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAAtt                                                                                                                                              <  2:1143352/150‑1 (MQ=255)
            cctgTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgt                                                                                                                                           <  2:1166012/147‑1 (MQ=255)
            ccGGATCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgt                                                                                                                                           <  2:120754/150‑1 (MQ=255)
                ttCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAATCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                       <  2:1685640/150‑1 (MQ=255)
                 tCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGa                                                                                                                                      <  2:766692/150‑1 (MQ=255)
                     gCTCATCTTATGTGTTGGGGAAAGAGTTTTAAACAGAAGATTAAGACTTGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCAAAATATCTTTTTATCACGCAAATAATTTGTGGTGATCTa                                                                                                                                  <  2:1119848/150‑1 (MQ=255)
                     ccagctctAATGTGACGGGGAAATCGGCTGAAACAGAAGATGAAGACTGGAATTTCTGAGTGTGTTAAATTCAAAAATATCACTACCCCCAGCAGGGAAATAATTCCCGCCAAAAAGCTTTTTATCACGCAAATAAATTGAGGTGATCTa                                                                                                                                  <  2:1112761/146‑1 (MQ=255)
                        cctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacac                                                                                                                               <  1:1506356/150‑1 (MQ=255)
                        cctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacac                                                                                                                               <  1:711909/150‑1 (MQ=255)
                        cctcTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTacaa                                                                                                                               >  2:169776/1‑149 (MQ=255)
                         ctctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAAAAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                              >  1:985253/1‑150 (MQ=255)
                          tctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTg                                                                                                                             >  2:1946019/1‑150 (MQ=255)
                          tctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTg                                                                                                                             >  2:1603136/1‑150 (MQ=255)
                             tATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTAGTGATCTACACTGATa                                                                                                                          >  1:431556/1‑150 (MQ=255)
                             tATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTATTATCACGCAAATAATTTGTGGTGATCTACACTGATa                                                                                                                          >  2:368447/1‑150 (MQ=255)
                                 tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActct                                                                                                                      >  1:929697/1‑150 (MQ=255)
                                 tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATActct                                                                                                                      >  1:1495032/1‑150 (MQ=255)
                                  gATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGCGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTg                                                                                                                     >  2:437803/1‑150 (MQ=255)
                                  gATGGGGAAAGCGGTTGAAACAGAAGAAGAAGACTGGAATTTCTGAGGGTGTTATTTTCTAAAATATCACTACCCGCAGCAGGGAAATAATTACCGCCAAATAGATATTTATCACTCAAATAATTTGTGGTGATCTCAACTCATACTCTg                                                                                                                     >  1:173062/1‑150 (MQ=255)
                                      gggAAAGCGGTTGAAACAGAAGATGAAGACTTGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCGCGACAATTTTTTTTTTTTATGTTAATGATTTTTTGGTGATCTACACTGATACGCTGTTGc                                                                                                                 <  2:214964/150‑1 (MQ=255)
                                      gggAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATGATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGc                                                                                                                 >  2:481759/1‑150 (MQ=255)
                                        gAAATCGGTTGAAACAGAAGTTGAAGACTGGAAATTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCat                                                                                                               <  1:456402/150‑1 (MQ=255)
                                        gAAAGCTGTTGAAACAGAAGATGATGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCat                                                                                                               <  1:992570/150‑1 (MQ=255)
                                         aaaGCGGTTGAAACAGAAGATGAAGCCTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAATCTGTTGCatt                                                                                                              >  2:1503664/1‑150 (MQ=255)
                                            gCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAAGAATTTGTGGTGATCTACACTGATACTCTGTTGCattatt                                                                                                           >  1:1080697/1‑150 (MQ=255)
                                             cGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCTCCAAATAGCATTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTc                                                                                                          >  1:119953/1‑150 (MQ=255)
                                                     aCAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATATCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATACGCCTGaaa                                                                                                  >  1:263163/1‑150 (MQ=255)
                                                      cAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCTCCAATTATTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAc                                                                                                 <  2:1477487/150‑1 (MQ=255)
                                                              gAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAatat                                                                                         >  1:1778544/1‑150 (MQ=255)
                                                                 gACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCa                                                                                      <  1:701625/150‑1 (MQ=255)
                                                                    tGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGc                                                                                   >  2:1759099/1‑150 (MQ=255)
                                                                     ggAATTTCTGAGGGTGTTATTTTCAAAAATATCCCTCCCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCg                                                                                  >  1:820689/1‑150 (MQ=255)
                                                                            cTGAGGGCGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCTCCAATTTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTc                                                                           <  2:1372148/150‑1 (MQ=255)
                                                                             tGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGTCGTTTTTTca                                                                          >  2:1943526/1‑149 (MQ=255)
                                                                              gtgggTGGTATTTTCAAAAATATCACTACCCGAAGCAGTGAAATATTTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGc                                                                         <  2:857158/148‑1 (MQ=255)
                                                                              gAGGGTGGTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGGCAAATATTTTTTTATATCTCAATTATTTTTTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGc                                                                         <  2:565736/150‑1 (MQ=255)
                                                                                 ggTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCACGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAATCCTCATTATTCAGGCGTTTTTTCGCTAt                                                                      >  1:170774/1‑150 (MQ=255)
                                                                                    gtTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCtt                                                                   >  2:642427/1‑150 (MQ=255)
                                                                                       aTTTTCAAAAATATCACTACCCGCAGCAGCGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAAAATTCAGGCGTTTTTTCGATATCTTAGa                                                                >  2:1355747/1‑150 (MQ=255)
                                                                                         tttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACa                                                              <  1:1747839/150‑1 (MQ=255)
                                                                                           tCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaa                                                            >  1:1009460/1‑150 (MQ=255)
                                                                                            cAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                           >  1:1977672/1‑150 (MQ=255)
                                                                                             aaaaaTATCACAACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTCCATTATTCTCCTGAAACCACAATATTAATGCTTTTTTTCGCTAACTTTTAaaaaaa                                                          >  2:690188/1‑150 (MQ=255)
                                                                                               aaaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAat                                                        >  1:416650/1‑150 (MQ=255)
                                                                                                aaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCTCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCATTGACAAAAAata                                                       >  2:247432/1‑150 (MQ=255)
                                                                                                 atatCACTACCCGCAGCAGGGAATTAATTCCCTCAAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAatat                                                      <  1:747307/150‑1 (MQ=255)
                                                                                                 atatCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAatat                                                      <  1:740098/150‑1 (MQ=255)
                                                                                                     cACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTTACAAAAAATATCAAc                                                  >  2:463940/1‑150 (MQ=255)
                                                                                                      aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATATTTTTTTATATGTTAAATATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                                 <  2:929696/150‑1 (MQ=255)
                                                                                                      aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTCCACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                                 <  2:1419457/150‑1 (MQ=255)
                                                                                                      aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                                 >  1:1836094/1‑150 (MQ=255)
                                                                                                         aCCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtc                                              <  1:1918771/150‑1 (MQ=255)
                                                                                                          cccGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtct                                             >  2:1646095/1‑150 (MQ=255)
                                                                                                          cccGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtct                                             >  2:1979185/1‑150 (MQ=255)
                                                                                                           ccGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAACTACACTGATACTCTGTTGAAATATTCGCCTGAAACCACAAAATTCAGGCGTATTTTCGCTAACTTTTAAAAAAAAAATAAACTTtcaa                                            >  2:1874280/1‑148 (MQ=255)
                                                                                                            cGCATCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTTATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCg                                           <  2:171224/150‑1 (MQ=255)
                                                                                                            cGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCg                                           >  1:1725102/1‑150 (MQ=255)
                                                                                                                 cagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTg                                      >  2:1912957/1‑150 (MQ=255)
                                                                                                                  agGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGc                                     <  1:1487141/150‑1 (MQ=255)
                                                                                                                  agGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGc                                     <  1:709398/150‑1 (MQ=255)
                                                                                                                    ggAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGctc                                   >  1:1435880/1‑150 (MQ=255)
                                                                                                                      aaaTAATTCCCGTCAAATAGATTTTTATCACTCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCtctc                                 <  1:368447/150‑1 (MQ=255)
                                                                                                                            ttCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCt                           >  1:906319/1‑150 (MQ=255)
                                                                                                                             tCCCGCCAAATTGTTTTTTATAATTAAAATATTTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCtt                          <  2:119953/150‑1 (MQ=255)
                                                                                                                             tCCCGCCAAATATTTTTTTATATGTTAATTAATTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCtt                          <  2:1593213/150‑1 (MQ=255)
                                                                                                                                cGCCAAATAGTTTTTTTTAAGTTAAATATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGACTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTtata                       <  2:985252/150‑1 (MQ=255)
                                                                                                                                cGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCAACACTGATAATCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGATATCTTTGGCAAAAAATATAACCTTTCTCGATTTCGTCTCAGCCCTtata                       >  2:857082/1‑150 (MQ=255)
                                                                                                                                         gCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCATCTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGaa              >  1:1103652/1‑150 (MQ=255)
                                                                                                                                               tATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCAATATCATTTACAAAAAATATCAACTTTCTCGATTTTATCTCAGACATTATATCACGGGAAATTccc        >  2:852156/1‑149 (MQ=255)
                                                                                                                                                  cACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCg     >  2:1221570/1‑150 (MQ=255)
                                                                                                                                                    cGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGTAAATTCCGGCGAt   >  1:855248/1‑150 (MQ=255)
                                                                                                                                                     gCAAATAATTTGTGGTGATCTACCCTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAtt  >  2:1714776/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATT  >  NC_000913/2804382‑2804680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: