Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,146,764 C→A 5.1% intergenic (‑27/+92) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,146,7640CA 5.1% 390.9 / 7.3 135intergenic (‑27/+92)yghW/yghXDUF2623 family protein/pseudogene, dienlactone hydrolase family
Reads supporting (aligned to +/- strand):  ref base C (53/74);  new base A (6/2);  total (59/76)
Fisher's exact test for biased strand distribution p-value = 7.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 135 total aligned reads displayed.

TGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGG  >  NC_000913/3146616‑3146911
                                                                                                                                                    |                                                                                                                                                   
  tgAGTAGCCTAATACTATACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTTAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATAAATTGTCata                                                                                                                                                  <  2:1934420/150‑1 (MQ=255)
        gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTTGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGc                                                                                                                                            >  1:451005/1‑150 (MQ=255)
        gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                                            >  2:2042226/1‑150 (MQ=255)
          cTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAgaga                                                                                                                                          >  1:1144735/1‑150 (MQ=255)
          cTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGa                                                                                                                                          >  1:1678090/1‑150 (MQ=255)
           tAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGat                                                                                                                                         >  2:1497759/1‑150 (MQ=255)
              tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTTGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatga                                                                                                                                      <  2:451005/150‑5 (MQ=255)
              tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAtt                                                                                                                                      <  2:103030/150‑1 (MQ=255)
              tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatga                                                                                                                                      <  1:1639047/150‑5 (MQ=255)
                cAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAtttt                                                                                                                                    <  2:1173523/150‑1 (MQ=255)
                 aaaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTa                                                                                                                                   <  1:814719/150‑1 (MQ=255)
                 aaaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGGTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTa                                                                                                                                   <  2:265582/150‑1 (MQ=255)
                  aaaCCGCGTTTATAATCGGCAGAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAg                                                                                                                                  <  2:1879467/150‑1 (MQ=255)
                  aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAg                                                                                                                                  <  1:1646905/150‑1 (MQ=255)
                   aaCCGCGTTTATAATAGGCACAGAATTTTGTCACATTAACCGCACGGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAgg                                                                                                                                 <  2:295046/150‑1 (MQ=255)
                    aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                <  2:1558555/150‑1 (MQ=255)
                    aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                >  1:1952759/1‑150 (MQ=255)
                    aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                <  1:1917946/150‑1 (MQ=255)
                        cgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAg                                                                                                                            <  2:1542323/150‑1 (MQ=255)
                          tttATAATCGGCACAGAATATTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTAATATATAGCGATATTTTAGGCAAAGaa                                                                                                                          >  2:1905964/1‑150 (MQ=255)
                           ttATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAAc                                                                                                                         >  2:832288/1‑150 (MQ=255)
                            tataATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCAGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACg                                                                                                                        <  2:1017843/150‑1 (MQ=255)
                               aaTCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACt                                                                                                                     <  1:1068040/150‑1 (MQ=255)
                               aaTCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACt                                                                                                                     >  2:1343134/1‑150 (MQ=255)
                                 tCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACttt                                                                                                                   <  1:693689/150‑1 (MQ=255)
                                    gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCGATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgc                                                                                                                >  2:1541542/1‑150 (MQ=255)
                                     cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcg                                                                                                               >  1:99522/1‑150 (MQ=255)
                                                 tCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAttttt                                                                                                   <  2:1952758/150‑1 (MQ=255)
                                                  cacaTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAtttttt                                                                                                  >  2:951424/1‑150 (MQ=255)
                                                   acaTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTc                                                                                                 <  2:450908/150‑1 (MQ=255)
                                                    caTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATAATGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                >  2:147259/1‑150 (MQ=255)
                                                    caTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCAATAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTATGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                <  2:712440/150‑1 (MQ=255)
                                                    caTTAACCGCACTGTCGGCATGAGTTGCTTTTAATCCCGCCATTAATCCTTTACCAAAATGGGTATTCATCGTTCACCTCATTCGCTATATATTGTCATCTATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                <  1:949840/150‑1 (MQ=255)
                                                      ttAACCGCACTGTCGGCATGCGTTGCTATTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATg                                                                                              <  1:654024/150‑1 (MQ=255)
                                                         aCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATAAATTTTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATTCtt                                                                                           >  2:1041882/1‑150 (MQ=255)
                                                          ccGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTg                                                                                          <  1:831634/150‑1 (MQ=255)
                                                             cACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGttt                                                                                       >  1:1043448/1‑150 (MQ=255)
                                                              aCTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTa                                                                                      <  2:1132386/150‑1 (MQ=255)
                                                              aCTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTa                                                                                      <  2:1867245/150‑1 (MQ=255)
                                                              aCTGTCGGCATGCGTTGCTTTTAATCCCGCAATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTa                                                                                      <  1:1164152/150‑1 (MQ=255)
                                                               cTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTaa                                                                                     >  2:416171/1‑150 (MQ=255)
                                                               cTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTaa                                                                                     >  1:239082/1‑150 (MQ=255)
                                                                tGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAg                                                                                    <  1:1126512/150‑1 (MQ=255)
                                                                 gTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGa                                                                                   >  2:1334133/1‑150 (MQ=255)
                                                                 gTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGAGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGa                                                                                   <  1:149860/150‑1 (MQ=255)
                                                                   cGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACg                                                                                 <  1:1532299/150‑1 (MQ=255)
                                                                   cGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACg                                                                                 >  2:1081529/1‑150 (MQ=255)
                                                                    ggCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGActt                                                                                >  1:457493/1‑148 (MQ=255)
                                                                       aTGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAAt                                                                             >  2:1679620/1‑150 (MQ=255)
                                                                              gcgtttAATCCCGCCATTAAGACTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAAAGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTTAGACGTAATCCCGaaa                                                                      >  1:1096406/4‑150 (MQ=255)
                                                                               cTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAag                                                                     >  2:1182766/1‑150 (MQ=255)
                                                                               cTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATAATTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAag                                                                     >  1:1153359/1‑150 (MQ=255)
                                                                                ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATATCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAaga                                                                    <  1:1783163/150‑1 (MQ=255)
                                                                                ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAaga                                                                    >  2:1180489/1‑150 (MQ=255)
                                                                                  ttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAgaga                                                                  <  2:230423/150‑1 (MQ=255)
                                                                                   tAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGAt                                                                 <  1:1526259/150‑1 (MQ=255)
                                                                                    aaTCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATAACGTAAGAGATa                                                                >  1:1327755/1‑150 (MQ=255)
                                                                                    aaTCCAGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATa                                                                <  2:1849253/150‑1 (MQ=255)
                                                                                       cccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGCACGCCTGTTGCGCACGCGATTTTTTCAGGCGTGTGGATGACGTATTCCGGTATGAGTTaaag                                                             >  2:1915520/1‑149 (MQ=255)
                                                                                        ccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAcc                                                            >  2:484383/1‑150 (MQ=255)
                                                                                        ccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAcc                                                            <  1:425876/150‑1 (MQ=255)
                                                                                             aTTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCa                                                       <  1:835635/150‑1 (MQ=255)
                                                                                              ttAACCCTTTACTAAACTTGTTATTCATCGTTCACATCATTCGCTATATATTTTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCTAACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAg                                                      <  1:349524/150‑1 (MQ=255)
                                                                                               tAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAgg                                                     <  2:1956124/150‑1 (MQ=255)
                                                                                                   ccTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGgagag                                                 <  2:1043447/150‑1 (MQ=255)
                                                                                                    cTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGgagaga                                                >  2:482904/1‑150 (MQ=255)
                                                                                                    cTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGgagaga                                                <  1:778880/150‑1 (MQ=255)
                                                                                                        aCCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAtt                                            <  2:174960/150‑1 (MQ=255)
                                                                                                        aCCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGAGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAtt                                            <  2:751116/150‑1 (MQ=255)
                                                                                                         ccAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAttt                                           <  2:811354/150‑1 (MQ=255)
                                                                                                         ccAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAttt                                           >  2:926293/1‑150 (MQ=255)
                                                                                                          cAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTa                                          <  2:1591900/150‑1 (MQ=255)
                                                                                                           aaaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTAt                                         <  1:1802912/150‑1 (MQ=255)
                                                                                                            aaaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATc                                        <  1:1686239/150‑1 (MQ=255)
                                                                                                                ggTTATTCATCGTTCCCCTCATTCGCTATAGATTGTCATATATAGCGATATTTTAGGCAAAGAACGAGTTTAGCGCACGCTATTTTTTCATCCTTGTGTAAGACGTAATCCCGTAAGAGAAAAACCTATCAGGAGAGATATTTATCGAAc                                    >  2:332806/1‑150 (MQ=255)
                                                                                                                ggTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAc                                    >  2:1521577/1‑150 (MQ=255)
                                                                                                                 gTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATAAAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAAACCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAAcc                                   <  1:1109632/150‑1 (MQ=255)
                                                                                                                    aTTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGCATTTTGCTCTCGCTATTTTTTCATACTTGTTTAAGACGTAATACCTTAAGAGTTAAACCTATCAGGAAAAATATTTATAGAACAAtt                                >  1:352960/1‑150 (MQ=255)
                                                                                                                    aTTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCAtt                                <  2:1678089/150‑1 (MQ=255)
                                                                                                                        aTCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAg                            <  1:1764010/150‑1 (MQ=255)
                                                                                                                          cGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCTTGCTTGTTTAAGACGTAATACCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtg                          >  1:1282105/1‑150 (MQ=255)
                                                                                                                             tCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTcc                       <  1:772421/150‑1 (MQ=255)
                                                                                                                              cacaTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGGTTAAGACGTAATCCAGTAAGAGATAAACCTAACAGGAGAGATATTTATCGAACCATTTCAGTGACCt                      >  2:1452206/1‑150 (MQ=255)
                                                                                                                                ccTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                    >  1:1232437/1‑150 (MQ=255)
                                                                                                                                ccTCATTCGCTATATATTGTCAGATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTTAGACGTAATCCCGTAAGAGATAACCCTATCAGGTGAGATATTTATAGAACCATTTCAGTGTCCttt                    >  1:1431051/1‑150 (MQ=255)
                                                                                                                                 cTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTg                   <  1:962377/150‑1 (MQ=255)
                                                                                                                                 cTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTg                   <  2:1988129/150‑1 (MQ=255)
                                                                                                                                 cTCATTCGCTATATATTGTCATATATAGCGATATTTTAGACAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTg                   <  2:812331/150‑1 (MQ=255)
                                                                                                                                   cATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTAATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGcc                 >  1:896850/1‑150 (MQ=255)
                                                                                                                                     atCGCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAg               >  2:610577/2‑150 (MQ=255)
                                                                                                                                       cGCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGc             >  1:1588072/1‑150 (MQ=255)
                                                                                                                                        gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGTCa            >  1:409947/1‑150 (MQ=255)
                                                                                                                                         caatatatTGTCATATAAAGCGATATTTTAGGCAAAGAACTACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACTTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCaa           <  2:115947/148‑1 (MQ=255)
                                                                                                                                           atatatTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGa         >  2:794083/1‑150 (MQ=255)
                                                                                                                                            tatatTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGAt        <  1:1343135/150‑1 (MQ=255)
                                                                                                                                              tatTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTACGACGTAATCCCGTAAGAGATAAACCTATCAGGATAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcg      <  2:1118426/150‑1 (MQ=255)
                                                                                                                                                ttGTCATTTATAGCGATATTTTAGGCAAAGACCGACTTTTGCGCACTCTATTTTTTCATGATTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTACTTTTCCAGGCAAGATcggc    <  1:1041883/150‑1 (MQ=255)
                                                                                                                                                ttGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggc    <  1:1519478/150‑1 (MQ=255)
                                                                                                                                                ttGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggc    <  1:712399/150‑1 (MQ=255)
                                                                                                                                                  gTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  >  2:1576646/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGG  >  NC_000913/3146616‑3146911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: