Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,708 A→G 5.2% intergenic (+25/+20) fadH → / ← higA 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,7080AG 5.2% 350.8 / 7.3 115intergenic (+25/+20)fadH/higA2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (76/33);  new base G (2/4);  total (78/37)
Fisher's exact test for biased strand distribution p-value = 8.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01

Only 100 of 117 total aligned reads displayed.

CTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCG  >  NC_000913/3233561‑3233857
                                                                                                                                                   |                                                                                                                                                     
cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                                                                     <  1:1933256/150‑1 (MQ=255)
 ttgcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACg                                                                                                                                                    <  2:81256/148‑1 (MQ=255)
 tGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACg                                                                                                                                                    <  1:977117/150‑1 (MQ=255)
  ggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCTCCGGATGCAACGc                                                                                                                                                   >  1:1914572/1‑150 (MQ=255)
  ggCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGc                                                                                                                                                   >  2:66785/1‑150 (MQ=255)
   gcgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTAGGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCa                                                                                                                                                  <  1:1570393/150‑1 (MQ=255)
    cgcAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCCGATGCAACGCAt                                                                                                                                                 >  1:1752792/1‑150 (MQ=255)
     gcAACCGCTGATTGATAGCGGGAAAACGGTGCTTTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCTCAGGAGATTAAAATCGATATTTGAAGCGCCGGATGCAACGAATc                                                                                                                                                >  2:1261076/1‑150 (MQ=255)
        aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCgg                                                                                                                                             >  2:1575854/1‑150 (MQ=255)
         ccGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGc                                                                                                                                            <  2:1451219/150‑1 (MQ=255)
          cGCTGATTGATCGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGGGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCGGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCa                                                                                                                                           <  2:1492596/150‑1 (MQ=255)
          cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcg                                                                                                                                           <  1:670401/150‑2 (MQ=255)
          cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcg                                                                                                                                           <  1:1329939/150‑2 (MQ=255)
          cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCa                                                                                                                                           <  1:1136029/150‑1 (MQ=255)
          cGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCCGCa                                                                                                                                           >  2:1364132/1‑150 (MQ=255)
          cGCTGATTGATAGCGGGAAAACGGGGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcg                                                                                                                                           <  1:679172/150‑2 (MQ=255)
           gCTGATTGATAGCGGGAAAACGGGGCAGTTAATTGGCGGCGGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCGGcgc                                                                                                                                          <  1:521014/150‑3 (MQ=255)
            cTGCTTGACAGCGGGAAAACGGTGCCTTTAAATCGCGGCTGCGATGGGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACg                                                                                                                                         <  1:235293/150‑1 (MQ=255)
                ttgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTcgc                                                                                                                                     >  1:511900/1‑150 (MQ=255)
                  gatAGCGGGAAAACGGTGCATTTTATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTTCACAGGGAACAAGGCTGGCGCTTGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGt                                                                                                                                   >  1:1056606/1‑150 (MQ=255)
                  gatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGt                                                                                                                                   >  2:234616/1‑150 (MQ=255)
                    tAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGttt                                                                                                                                 >  1:1494739/1‑149 (MQ=255)
                    tAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGAGGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCACCGGATGCAACGCATCCGGCACGTCGCGTTa                                                                                                                                 <  2:1611818/150‑1 (MQ=255)
                      gCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAAt                                                                                                                               >  2:1925153/1‑150 (MQ=255)
                        gggAAAACGGTGCATTTAATTGGCTGCTGCGATGTGGCTATGGAGCTGGACGCAAGACGGGCAAATGCCCAGGGAACACGGATGGCGCTGGAGATTTAAATCGTTATTTGAAGCTCCGGATGCAAAACATCCTGCACGTCGCAGTAATCa                                                                                                                             >  2:1961654/1‑150 (MQ=255)
                         ggAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCATGGAACACTGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGGCGCGTTAATCaa                                                                                                                            >  1:266020/1‑150 (MQ=255)
                             aaCGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaaa                                                                                                                        >  2:1666143/1‑150 (MQ=255)
                                ggTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACaa                                                                                                                     >  2:1132504/1‑150 (MQ=255)
                                     aTTTTATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCACAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCTATAAACAAGGCgg                                                                                                                >  1:283215/1‑150 (MQ=255)
                                         aaTTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGgaga                                                                                                            >  1:488111/1‑150 (MQ=255)
                                         aaTTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGgaga                                                                                                            >  1:891550/1‑150 (MQ=255)
                                          aTTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGGACGTCGCGTTAATAAATTAACAAGGCGGGAGaa                                                                                                           >  1:1989728/1‑150 (MQ=255)
                                            tGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAAt                                                                                                         >  1:1416440/1‑150 (MQ=255)
                                             ggcggcTGCGATGTGGCTATGGAGCTGGGCGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATg                                                                                                        >  2:417380/1‑150 (MQ=255)
                                             ggcggcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGCACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATg                                                                                                        >  2:1193862/1‑150 (MQ=255)
                                                ggcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCg                                                                                                     >  2:1888112/1‑150 (MQ=255)
                                                 gcTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGCAGCAACGCATCCGGCACGTAGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGa                                                                                                    >  1:1998465/1‑150 (MQ=255)
                                                     cGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGGGATGCCGAAGcg                                                                                                >  1:1310531/1‑150 (MQ=255)
                                                          tgGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGCACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCCTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTg                                                                                           >  1:112731/1‑150 (MQ=255)
                                                           ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCTGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                                                                          >  2:666764/1‑150 (MQ=255)
                                                           ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                                                                          <  2:1475467/150‑1 (MQ=255)
                                                           ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGGGAATCAATAAACAAGGCGGGAGAACTGCCGAATCGCGGTGc                                                                                          >  2:335065/1‑150 (MQ=255)
                                                           ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCGGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                                                                          <  2:1470300/150‑1 (MQ=255)
                                                             cTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCCCGTCGCGTTAATCCAAAAACAACGCGGGAGCAATCCCGAAACGCGTTGCCa                                                                                        >  2:119927/1‑150 (MQ=255)
                                                             cTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa                                                                                        >  1:670203/1‑150 (MQ=255)
                                                             cTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa                                                                                        >  1:83573/1‑150 (MQ=255)
                                                             cTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa                                                                                        >  1:886413/1‑150 (MQ=255)
                                                             cTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa                                                                                        >  1:383499/1‑150 (MQ=255)
                                                                  gAGCTGGCCGCACGACGGGCAATTGCCCAGGGAACACGGCTGGTGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttt                                                                                   >  2:1472424/1‑150 (MQ=255)
                                                                    gCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCCCGTCGCGGTAATCAATAAACAAGGCGGGAGAAATGCCGCATCGCGGTGCCAAtttttt                                                                                 >  1:1776600/1‑150 (MQ=255)
                                                                      tgcacccACGACGGTAAATTCCCCCGGTAACCGGGGTGGCGGTGTATTTTAAATTCTTTTTTTCACGCGGGGGTTCCACCTCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAg                                                                               <  2:916794/144‑1 (MQ=255)
                                                                          cGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTTATCAATAAACAAGGCGGGTGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtg                                                                           >  2:1567130/1‑150 (MQ=255)
                                                                          cGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtg                                                                           >  2:559371/1‑150 (MQ=255)
                                                                                acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCCTCCGGCACGTCGCGTTTATAAATAAACAAGGCGGGAGAAAAGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTACAg                                                                     >  1:1737141/1‑150 (MQ=255)
                                                                                acgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGGCGCGTTAATCAATAACCAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAg                                                                     >  1:1314603/1‑150 (MQ=255)
                                                                                 cgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGc                                                                    >  1:906502/1‑150 (MQ=255)
                                                                                   ggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCCGCGt                                                                  >  1:121250/1‑150 (MQ=255)
                                                                                    gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                                                                 >  1:1183793/1‑150 (MQ=255)
                                                                                           cccAGGTAACACGGCTGGCGTTGTTTATTTAAATCGTTATTTGAAGCGCGGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc                                                          <  2:383499/150‑1 (MQ=255)
                                                                                           cccAGGGAACACGGCTGGCGCTGGATATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc                                                          <  2:290456/150‑1 (MQ=255)
                                                                                           cccAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc                                                          >  1:1375473/1‑150 (MQ=255)
                                                                                            ccAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAATTTTTAGCGTGTTCCAGCATTAATTTAct                                                         >  1:628989/1‑150 (MQ=255)
                                                                                             cAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCctc                                                        >  2:831299/1‑150 (MQ=255)
                                                                                               gggAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCtt                                                      >  2:1477561/1‑150 (MQ=255)
                                                                                                     ccGGCTGGCGCGGGTTATTTAAATCGTTATTTGAAGCGCCGGAGGCACCGAATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCt                                                <  2:505208/149‑1 (MQ=255)
                                                                                                     acGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTACAGCGTTAATTTCCTCTTCCCGCt                                                >  1:999875/1‑150 (MQ=255)
                                                                                                      cGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCCCGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTc                                               >  1:660465/1‑150 (MQ=255)
                                                                                                      cGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCATTAAACAAGGCGGGAGAAATGCCGAATAGCGTTGCCAATTTTTTAGCTTGTTCCAGCGGTAATTTCCTCTTCCCGCta                                               >  1:989846/1‑149 (MQ=255)
                                                                                                       ggcgggggcgggACATTAAAATGGTTATTTCACGCCCCGTATCCACCGCACCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGGGTTCCAGCGTTAATTTCCTCTTCCCGCTCa                                              <  2:1828991/150‑1 (MQ=255)
                                                                                                       ggcgggcgCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCa                                              <  2:3807/150‑1 (MQ=255)
                                                                                                           ggCGCTGTATATTAAAATGTTTATTTTCAGCGCCGTATTCAACTCACGCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc                                          <  1:1633338/150‑1 (MQ=255)
                                                                                                            gcgcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcg                                         >  1:1631906/1‑150 (MQ=255)
                                                                                                             cgcTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGTGAGAAATGCCCAATCGCGTTGCCAATTTTTAAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgc                                        >  2:303208/1‑150 (MQ=255)
                                                                                                                tGGATATTTAAAACGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTg                                     <  2:1414595/150‑1 (MQ=255)
                                                                                                                gggAGATTTATATCGTTATTGGACGGGCCGGATGCACCGCAGCGGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTg                                     <  2:551527/149‑1 (MQ=255)
                                                                                                                gggAGATTTAAATCTTTATTTGAAGCCCCGGAGGCAACGCACCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTg                                     <  2:559040/149‑1 (MQ=255)
                                                                                                                 gtataTTTAAATGGTTATTTGAAGCGCCGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGa                                    <  2:891549/146‑1 (MQ=255)
                                                                                                                 ggAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTgc                                    >  2:2049520/1‑149 (MQ=255)
                                                                                                                  gagaTTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGac                                   <  2:1217649/150‑1 (MQ=255)
                                                                                                                         aaaTCTTTATTTGAAGCGCGGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAg                            <  1:1125668/150‑1 (MQ=255)
                                                                                                                            tCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTTATCAAAAAACAAGGCGGGAGAAATGCCGGATCGCGTTGCCAATTTTTTAGCGTTTTGCAGCGTTTATTTCCAGTTACCGCTCAAAAAGGGTGCAGCCATAGAtt                         >  2:1927892/1‑150 (MQ=255)
                                                                                                                              gTTATTTGAAGCGCCGGATTCAACGCATCCGGCACGTCGCGTTAATAAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTTGCGTGTTACAGCGTTAATTTCCTATTCCCGCTCAAAACGCGGGCCACCATAGATTTa                       >  2:434196/1‑150 (MQ=255)
                                                                                                                              gTTATTTGAAGCGCCGGATGTAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTa                       >  2:331274/1‑150 (MQ=255)
                                                                                                                              gTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTa                       <  2:1183792/150‑1 (MQ=255)
                                                                                                                                 tttttccgcgcGGTATGCAACGCTGCGGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTg                    <  2:1696492/143‑1 (MQ=255)
                                                                                                                                  tttGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACAATAGATTTACTGc                   >  2:1060168/1‑150 (MQ=255)
                                                                                                                                    ttaaGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa                 <  1:1154163/148‑1 (MQ=255)
                                                                                                                                    tGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGCCACCATAGATTTACTGCCa                 >  1:1213180/1‑150 (MQ=255)
                                                                                                                                    tGAAGCGCCGGATGCAACGCATCAGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGATTCGCGGTGCCAATTTTTTGGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGACa                 >  1:289338/1‑150 (MQ=255)
                                                                                                                                         cgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGTGTGACACCATAGATTTACTGCCAATTTc            <  2:221088/150‑1 (MQ=255)
                                                                                                                                          gcCGGATGCGTTGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCACGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGACAATTTca           >  1:1563583/1‑149 (MQ=255)
                                                                                                                                          gcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTcc           >  2:1690072/1‑150 (MQ=255)
                                                                                                                                            cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGACTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCACGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg         >  2:303577/1‑150 (MQ=255)
                                                                                                                                            cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg         >  2:1138549/1‑150 (MQ=255)
                                                                                                                                             gtaTGAAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc        <  2:1900176/148‑1 (MQ=255)
                                                                                                                                             ggATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc        >  1:783390/1‑150 (MQ=255)
                                                                                                                                              ttggaaaCGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCa       <  1:1879173/145‑1 (MQ=255)
                                                                                                                                                 gCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGAt    >  1:391658/1‑150 (MQ=255)
                                                                                                                                                  cAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATc   <  2:886412/150‑1 (MQ=255)
                                                                                                                                                   aaCGAAACCGGCACGTCGCGTTAATCAATACACCAAGCGGGAGAAATTCCGAATCGCGATGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACAGAATAGATTTACTGACAATTACCGGCAGAtag  >  1:44320/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCG  >  NC_000913/3233561‑3233857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: