Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 347,707 C→A 9.1% G246V (GGG→GTG)  prpR ← propionate catabolism operon regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913347,7070CA 9.1% 267.9 / 3.2 105G246V (GGG→GTG) prpRpropionate catabolism operon regulatory protein
Reads supporting (aligned to +/- strand):  ref base C (47/46);  new base A (3/9);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAAC  >  NC_000913/347559‑347856
                                                                                                                                                    |                                                                                                                                                     
cAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTcccc                                                                                                                                                      >  1:538946/1‑150 (MQ=255)
 aGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTccccc                                                                                                                                                     <  2:904605/150‑1 (MQ=255)
 aGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTccccc                                                                                                                                                     <  2:2019893/150‑1 (MQ=255)
  gTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCt                                                                                                                                                    <  2:759696/150‑1 (MQ=255)
    tCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtca                                                                                                                                                  <  1:664186/150‑1 (MQ=255)
     cTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtcaa                                                                                                                                                 >  2:731698/1‑150 (MQ=255)
     cTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtcaa                                                                                                                                                 >  2:403107/1‑150 (MQ=255)
      tGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtcaat                                                                                                                                                <  1:1031249/150‑1 (MQ=255)
       gCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtcaatc                                                                                                                                               <  1:1900118/150‑1 (MQ=255)
       gCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtcaatc                                                                                                                                               <  2:1342833/150‑1 (MQ=255)
        cTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCtcaatca                                                                                                                                              <  2:1007635/150‑1 (MQ=255)
           ccAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAaca                                                                                                                                           >  1:550645/1‑150 (MQ=255)
            cagcagCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAacac                                                                                                                                          >  1:1914163/1‑150 (MQ=255)
            cagcagCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAacac                                                                                                                                          >  2:897319/1‑150 (MQ=255)
               cagcGATTCGGCAATCGCCCCGCAGTTGACGGCAACAACCGGATGCGACCTTTTGCCCTTGCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACAccgc                                                                                                                                       <  1:1132249/150‑1 (MQ=255)
                 gcGATTCGGCCATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGACGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACAccgccg                                                                                                                                     <  1:321773/150‑1 (MQ=255)
                  cGATTCGGCAATCGCCCCGTAGTTGACGGCAACATACGGATGCGACTTTTTGCCCTGTCGCGCAGCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACcgccgc                                                                                                                                    <  1:161412/150‑1 (MQ=255)
                   gATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACcgccgcc                                                                                                                                   >  2:1826361/1‑149 (MQ=255)
                     atCGGCAATCGCCCCTCAGTTGACGGCAAGAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGAGGGCAAAATATTCCCGATTAATCGCCTGGGCCGCTTTTTTTTTTAATGTTACGGTTTCCACCTATATCTACACCGCCGCACt                                                                                                                                 <  2:762417/149‑1 (MQ=255)
                       cGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTgg                                                                                                                               <  1:2027365/150‑1 (MQ=255)
                        ggCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGa                                                                                                                              >  1:1872646/1‑150 (MQ=255)
                        ggCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGATTTTTTAATGTTACGGTTTCCACCTATATCAACACCGCCGCACTGGa                                                                                                                              <  2:1658459/150‑1 (MQ=255)
                         gCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGAGGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAg                                                                                                                             >  1:1029274/1‑150 (MQ=255)
                          cAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCGATGCCCGGCGCCGGTTCCAGCTCACTCCACACCGACGGACTgggtc                                                                                                                            >  2:349713/1‑147 (MQ=255)
                          cAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGAATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCTCCGCACTGGAGc                                                                                                                            <  1:1593451/150‑1 (MQ=255)
                             tCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCggg                                                                                                                         <  1:1063417/150‑1 (MQ=255)
                             tCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCggg                                                                                                                         <  1:899218/150‑1 (MQ=255)
                              cGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCATTTTTTTGAATGTTACGGTTTCCACCTATATCTACACCGCCGCACTGGAGCGGGc                                                                                                                        <  2:606441/150‑1 (MQ=255)
                               gCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCAAGTTTTTTTAATGTTACGGTTTCCACCTATATCAACACCGCCGCACTGGAGCGGGCa                                                                                                                       <  2:1465463/150‑1 (MQ=255)
                                ccccGCAGTTGACGGCAACAACCGGATGCGCCTTTTTGCCCTGGCGCGCATCGTGGCGGGCAAAATAATCCCGATGAATCGCCGGGGCCGCCAGCTCTTTGCCCGTCCCAGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCAt                                                                                                                      <  1:125260/150‑1 (MQ=255)
                                  ccGCAGTTGACGGCCACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCACTCACCACCGCCGCCCTGGAGCGGGCATAc                                                                                                                    >  1:656917/1‑150 (MQ=255)
                                  ccGCAGTTGACGGCAACAAACGGATGCGTCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGTTCTTTGACCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATAc                                                                                                                    <  2:434886/150‑1 (MQ=255)
                                   cGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATAATCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACa                                                                                                                   <  1:1264887/150‑1 (MQ=255)
                                       gTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCaa                                                                                                               <  1:64784/150‑1 (MQ=255)
                                            cGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAg                                                                                                          >  2:868327/1‑150 (MQ=255)
                                              gCAACACACGGATGCGACTTTTTGGCCTGGCGCGCATCGTGGCGTGCAAAATATTCCCGACGAATCGCCTGGGCCGCCAGCTCTATGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTc                                                                                                        <  1:40533/150‑1 (MQ=255)
                                                aacaacCGGATGCGACGTTGTGCCCTGTCGCGCATCGGGGCGGGCAAAATATTCCCGCGGCATCGCCGGGGCCGCCAGCGCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATCGGCTg                                                                                                      <  1:1567822/150‑1 (MQ=255)
                                                 acaaACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGc                                                                                                     <  2:1635747/150‑1 (MQ=255)
                                                  caaACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGcc                                                                                                    >  2:2048917/1‑150 (MQ=255)
                                                    accGGAGGCGCCTTTTTGTCCGGTCGCGCATCGGGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGATTTTTTTTTTAATGATACGGGTTCCACCGATATCTACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGt                                                                                                  <  2:1806941/148‑1 (MQ=255)
                                                         aTGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTg                                                                                             <  1:1052020/150‑1 (MQ=255)
                                                          tGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGt                                                                                            >  2:466553/1‑150 (MQ=255)
                                                            cGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCCCTGGAGCGGGCATAGAGCAAAATAGTCTGCCGTACTTGTTc                                                                                          >  2:900575/1‑150 (MQ=255)
                                                            cGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTc                                                                                          >  2:42937/1‑150 (MQ=255)
                                                             gACTTTTTGCCCTGTCGCGCATAGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTcc                                                                                         <  1:1034188/150‑1 (MQ=255)
                                                              aCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCTACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCa                                                                                        <  2:1624132/150‑1 (MQ=255)
                                                              aCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCa                                                                                        >  1:1661301/1‑150 (MQ=255)
                                                              aCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCa                                                                                        >  2:433877/1‑150 (MQ=255)
                                                              aCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCa                                                                                        >  2:2033223/1‑150 (MQ=255)
                                                              aCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTATTTTCACGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCa                                                                                        <  2:1077881/150‑1 (MQ=255)
                                                              aCTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTATTTGCCCGTCACCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCa                                                                                        <  2:453097/150‑1 (MQ=255)
                                                                     gCCCTGTCGCGCATCGTGGCGGGCTAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTTCCCGTCCCCGTTTCCACCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTgtgg                                                                                 <  2:713668/150‑1 (MQ=255)
                                                                     gCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACCCCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTgtgg                                                                                 >  1:762060/1‑150 (MQ=255)
                                                                       ccTGTCGCGCAGCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTgtggtg                                                                               <  1:847799/150‑1 (MQ=255)
                                                                        cTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGa                                                                              <  1:1325291/150‑1 (MQ=255)
                                                                         tGTCGCGCATCGTGGCGGACAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGAt                                                                             >  2:1143100/1‑150 (MQ=255)
                                                                            cgcgCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTGCCCCTCAATCAACACCGCCGCACTGGCGCGGGCAGACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGgtgagtga                                                                          >  1:673096/1‑150 (MQ=255)
                                                                            cgcgCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGa                                                                          >  2:1433435/1‑150 (MQ=255)
                                                                                cATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCgg                                                                      >  1:611561/1‑149 (MQ=255)
                                                                                   cGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCTCCAGCTCTTTGGCCGTCACCGTTTCCCACTCTATCATCACCGCCGCACTTGAGCTGGCATACAGCATAAAAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGct                                                                   <  1:499168/150‑2 (MQ=255)
                                                                                   cGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCATCTCTTTGACCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCa                                                                   <  2:1214791/150‑1 (MQ=255)
                                                                                      ggCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCatat                                                                >  2:1549951/1‑150 (MQ=255)
                                                                                        cGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAGTAGTCTGCCGTACTTGGTCCATCTGTGGTGCTTGACCGAGCATATCg                                                              >  1:1051384/1‑150 (MQ=255)
                                                                                        cGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAAGAGTCTGCCGTACTTGTTCCAGCTGTGGTGATGGACCGAGCATATCg                                                              >  2:1367627/1‑150 (MQ=255)
                                                                                         gggCAAAATATTCCCGATGAATCTCCTGGGCCGAGATTTTTTTTAATGTTACGGTTTCCACCTATATCTACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGc                                                             <  2:1783370/150‑1 (MQ=255)
                                                                                         gggCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGTTTTTTTAATGTTACGGTTTCCACCTATATCTACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGc                                                             <  2:1305785/150‑1 (MQ=255)
                                                                                          ggCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGcc                                                            <  1:1791446/150‑1 (MQ=255)
                                                                                            cAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCa                                                          <  1:1143101/150‑1 (MQ=255)
                                                                                                     cccGATGAATCGCCTGGGCCGCCAGCGCTTTGCCCGGCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGGTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAAc                                                 <  1:1114547/150‑1 (MQ=255)
                                                                                                      ccGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACg                                                >  1:868176/1‑150 (MQ=255)
                                                                                                          tGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGGGTCCCCCTCAATCACCACCGCCGCCCTGGCGCGGGCAGCCAGCAATATAGGCTGCCGTACTTGTTCCAGCTGTGGTGATGGACCGAGCATATCGCCCCGCACGTCACGCGTa                                            >  1:1233531/1‑150 (MQ=255)
                                                                                                           gAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTAc                                           <  1:1437262/150‑1 (MQ=255)
                                                                                                             aTCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGc                                         >  1:1994959/1‑150 (MQ=255)
                                                                                                               cGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCCCGTAACGAGTACGCAg                                       >  1:1406072/1‑150 (MQ=255)
                                                                                                                ggcTGGGCCGCCAGCTCTTTGCCCGTCCCCGTGTCGCCCTCAATCACCACCGCCGCACTGGAGCGGGCATACAGCATAATAGTCTGCCGGACTAGTACCATCTCTGGTTAATGACCGAGCATATCGCCCCGCACGTAACGAGTACGCacg                                      >  1:630414/3‑148 (MQ=255)
                                                                                                                gCCTGGGCCGCCAGCTCTTTCCCCGTCCCCGTTTCCACCTCAATCAACACCCCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTACATATGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGAAgg                                      >  2:168380/1‑150 (MQ=255)
                                                                                                                  cTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGc                                    <  1:1758437/150‑1 (MQ=255)
                                                                                                                      gccgccAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTg                                >  1:1369175/1‑150 (MQ=255)
                                                                                                                      gccgccAGCTCTTTGCCCGTCCCCGATTCCACCTAAATCAACACCGCCGCACTCTAGCCGTCATACAGCAAAATAGTCTGCCGTAATTATTCACTCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTAAGCAGGGCTTTg                                >  1:1082036/1‑150 (MQ=255)
                                                                                                                       ccgccAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGc                               >  2:1613256/1‑150 (MQ=255)
                                                                                                                           cAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACCCCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGACCAGCACGTAACGAGTACGCAGGGCGTTGCGGGt                           >  2:1119665/1‑150 (MQ=255)
                                                                                                                             tatctTTTACCGTAACCGTTTCCACCTATATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTgg                         <  2:1339607/148‑1 (MQ=255)
                                                                                                                             gCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTgg                         <  1:1613665/150‑1 (MQ=255)
                                                                                                                                ctTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGCCCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCAt                      >  1:939756/1‑150 (MQ=255)
                                                                                                                                ctTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCAt                      >  2:1072365/1‑150 (MQ=255)
                                                                                                                                 tttGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGCCCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCagc                     >  1:1705190/1‑148 (MQ=255)
                                                                                                                                  ttGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATcc                    >  2:2037095/1‑149 (MQ=255)
                                                                                                                                   tGCCCGTCCCCGTTTCCCCCTCAATCTACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGt                   >  2:783080/1‑150 (MQ=255)
                                                                                                                                    gCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTg                  <  1:590253/150‑1 (MQ=255)
                                                                                                                                    gCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTg                  >  2:1479018/1‑150 (MQ=255)
                                                                                                                                    gCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACGGGAGCGGGCATACAGCAAAATAGGCTGCCGTACTTGTGCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTg                  <  1:992718/150‑1 (MQ=255)
                                                                                                                                     cccGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTgt                 >  1:445460/1‑149 (MQ=255)
                                                                                                                                     cccGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGa                 >  2:1964120/1‑150 (MQ=255)
                                                                                                                                        ggccccGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAgtg              <  2:1358970/148‑1 (MQ=255)
                                                                                                                                        gTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAgtg              <  2:1941829/150‑1 (MQ=255)
                                                                                                                                          ccccGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGtt            >  2:1764411/1‑150 (MQ=255)
                                                                                                                                           cccGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTa           <  2:1255694/150‑1 (MQ=255)
                                                                                                                                                ttCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGCACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCg      <  2:1029273/150‑1 (MQ=255)
                                                                                                                                                 tCCCCCTCCATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGt     <  1:188389/150‑1 (MQ=255)
                                                                                                                                                    cccTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAAc  >  2:707794/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGTTCTGCTTCCAGCAGCGATTCGGCAATCGCCCCGCAGTTGACGGCAACAAACGGATGCGACTTTTTGCCCTGTCGCGCATCGTGGCGGGCAAAATATTCCCGATGAATCGCCTGGGCCGCCAGCTCTTTGCCCGTCCCCGTTTCCCCCTCAATCAACACCGCCGCACTGGAGCGGGCATACAGCAAAATAGTCTGCCGTACTTGTTCCATCTGTGGTGATTGACCGAGCATATCGCCCAGCACGTAACGAGTACGCAGGGCGTTGCGGGTGGCATCGTGAGTGTTATGGCGTAAC  >  NC_000913/347559‑347856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: