Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,263,500 G→A 5.1% P54L (CCG→CTG)  tdcD ← propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,263,5000GA 5.1% 294.0 / 3.4 104P54L (CCG→CTG) tdcDpropionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (37/59);  new base A (2/3);  total (42/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

Only 100 of 108 total aligned reads displayed.

CTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGA  >  NC_000913/3263351‑3263649
                                                                                                                                                     |                                                                                                                                                       
cTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCg                                                                                                                                                         >  1:521641/1‑150 (MQ=255)
 ttCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGTAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCgg                                                                                                                                                        <  1:1794374/150‑1 (MQ=255)
  tCATCGGTAATAATGGCGGACTCGGTATAAATACTGCCGCCGTGAGCGAGGCGGTGGCCAATTAACGCCACACTGGCAATTAAATTCCGTGTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTGCGTAGCTGTGGTGAGCCAGCGGt                                                                                                                                                       <  1:392071/150‑1 (MQ=255)
  tCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCggg                                                                                                                                                       >  1:567304/1‑149 (MQ=255)
      cGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                   <  1:774555/150‑1 (MQ=255)
      cGGTAATAATGGCGGACTCGGTAAAAAAACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTg                                                                                                                                                   <  2:75019/150‑1 (MQ=255)
       ggTAATACTGGCGGACTCGGAAAAAATACTGCCGCCGGGAGCGATGCGGTGGCCAATTAATGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCCATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                                  <  1:1991435/150‑1 (MQ=255)
       ggTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTgg                                                                                                                                                  >  1:2017579/1‑150 (MQ=255)
        gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGGCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                                 <  1:174913/150‑1 (MQ=255)
        gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                                 <  1:1649059/150‑1 (MQ=255)
        gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                                 <  1:1880910/150‑1 (MQ=255)
        gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGc                                                                                                                                                 <  1:1390061/150‑1 (MQ=255)
        gTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCACCGGTTCTGGc                                                                                                                                                 >  2:1305595/1‑150 (MQ=255)
          aataatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGctc                                                                                                                                               <  2:271029/150‑1 (MQ=255)
          aataatGGCGGACTAGGTAAAAATCCTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGATCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctc                                                                                                                                               <  2:511640/150‑1 (MQ=255)
            taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCCGCGGGGCTGGCtctc                                                                                                                                            >  1:2027968/1‑150 (MQ=255)
            taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCtctc                                                                                                                                            <  2:1972126/150‑1 (MQ=255)
            taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCtctc                                                                                                                                            <  2:1355833/150‑1 (MQ=255)
            taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                             <  2:260613/150‑3 (MQ=255)
            taatGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCACCGCTGGctcac                                                                                                                                             <  2:849984/150‑3 (MQ=255)
             aatGGCGGACTAGGTAAAAATACTGCCGCCGTGAGAGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTcc                                                                                                                                           >  2:1209098/1‑150 (MQ=255)
              atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccc                                                                                                                                          <  2:499769/150‑1 (MQ=255)
              atGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTccc                                                                                                                                          <  2:499771/150‑1 (MQ=255)
                 gCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCa                                                                                                                                       <  2:690974/150‑1 (MQ=255)
                    gACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCCGCGGTGCTGGGGGTCCCCCAttt                                                                                                                                    >  1:1275093/1‑150 (MQ=255)
                    gACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAttt                                                                                                                                    <  1:1391942/150‑1 (MQ=255)
                     aCTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCCCACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCCCCCGGGCTGGGTGGCCCCCaaata                                                                                                                                   >  1:1524294/1‑146 (MQ=255)
                      cTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTAc                                                                                                                                  >  1:134080/1‑150 (MQ=255)
                         ggTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCCTTTACCGa                                                                                                                               >  2:730219/1‑150 (MQ=255)
                          gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGAt                                                                                                                              <  2:428156/150‑1 (MQ=255)
                          gTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGAt                                                                                                                              <  2:137622/150‑1 (MQ=255)
                             aaaaTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAg                                                                                                                           <  2:725390/150‑1 (MQ=255)
                                aTACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAACCAACGGGGGAGGCAATACCCCCATTACCGAAAAGaaa                                                                                                                        >  1:887249/1‑149 (MQ=255)
                                   cTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGCGCCAGCGGTGCTGGCTCTACCCCATTTACGGATATGAATGCa                                                                                                                     >  1:133919/1‑150 (MQ=255)
                                        gccgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCATAGCTGTGGTGAGCCAGCGGTGCTGGCGGTCCCCCCATTTAGGGTTATAAATGATtttt                                                                                                                 >  2:699519/1‑150 (MQ=255)
                                          cgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCg                                                                                                              <  2:1966824/150‑1 (MQ=255)
                                          cgTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCg                                                                                                              <  2:1428802/150‑1 (MQ=255)
                                           gTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCATATGCATTTGCCTTCAATGGACCTTCGTAGCTGTGGTGAGCCCGCGGTGCTGGCTGTCCGCCATTTACGGCTAAGAATGCATTTTCCgg                                                                                                             >  2:83689/1‑149 (MQ=255)
                                           gTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCGTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                             <  2:130949/150‑1 (MQ=255)
                                           gTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGa                                                                                                             <  1:719719/150‑1 (MQ=255)
                                            tGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTGTCCCCCATTTACGGATAAGAATGCATTTTCCGAg                                                                                                            >  2:151831/1‑150 (MQ=255)
                                               gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTa                                                                                                         <  2:1019399/150‑1 (MQ=255)
                                               gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAATTTCGGATTAGAAAGCATTTTCCGAGttt                                                                                                         >  2:815915/1‑149 (MQ=255)
                                               gCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCAGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTa                                                                                                         >  2:1345631/1‑150 (MQ=255)
                                                  aTGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCATCGTAGCTGTGGTGAGCCACCCGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCAATTTCCGAGTTAATa                                                                                                     >  2:1117598/1‑150 (MQ=255)
                                                        gggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCg                                                                                               <  1:1511602/149‑1 (MQ=255)
                                                         ggCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTCAATACCGTCgg                                                                                              <  1:1470529/150‑1 (MQ=255)
                                                          gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                             <  1:1493627/150‑1 (MQ=255)
                                                          gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAAAACCGTCGGc                                                                                             >  2:273137/1‑150 (MQ=255)
                                                          gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAATTAAGGATAATAAAGCATTTTACGAGTTAATAACGTCGGc                                                                                             >  2:1640586/1‑150 (MQ=255)
                                                          gCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGATCATATGCTATTTCCTTCAATGCTCCTTCGTAGCGGTGGTGAGCCTGCGGTGCTGGGTCTCCCCCATATACGGATTAGAATGCATTTTCCGAGTTAATACCGTCGGc                                                                                             <  2:86595/150‑1 (MQ=255)
                                                           tcAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGCGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCa                                                                                            <  2:173676/149‑1 (MQ=255)
                                                           ccAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCa                                                                                            >  2:1340368/1‑150 (MQ=255)
                                                               ttAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAc                                                                                        >  1:1179110/1‑150 (MQ=255)
                                                                tAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATTCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATAcc                                                                                       <  2:1433796/150‑1 (MQ=255)
                                                                    gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGTTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                   <  2:1880265/150‑1 (MQ=255)
                                                                    gCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGAc                                                                                   <  1:1149493/150‑1 (MQ=255)
                                                                       acacTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACAtt                                                                                <  1:857087/150‑1 (MQ=255)
                                                                        cacTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTa                                                                               >  1:1102369/1‑150 (MQ=255)
                                                                          cTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCAAACGTGCGGGCAAAACCCCAATTACAGATAAAAAAGAATTTTAACAATTAAAAACGTAGGCAAAACCAGGCAATaaa                                                                             >  1:1019146/1‑149 (MQ=255)
                                                                           tggcATTTAACTTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGGGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATa                                                                            <  1:352680/147‑1 (MQ=255)
                                                                             tCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACt                                                                          <  2:1610579/150‑1 (MQ=255)
                                                                              cATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTACGAGTTAATACCGTCGGCAATACCTGACATTAATACtt                                                                         >  2:1064319/1‑150 (MQ=255)
                                                                              cATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGAAAAGAAAGCATTTTCCGAGTTAAAACCGTCGGCAATAACTGACAATAATAcat                                                                         >  2:860372/1‑148 (MQ=255)
                                                                               aTTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTc                                                                        >  2:68259/1‑150 (MQ=255)
                                                                                tttAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCGTCGTAGCTGTGGTGAGCCAGCGGTGCTGCCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAAACGTGACATTAATTCTTca                                                                       >  2:1934378/1‑150 (MQ=255)
                                                                                        ccGTTTTTCCAGTTCTAATGCAATTGCCTTCAATGGACCTTCGTAGCTGTGGTGTGCCTGAGGTGCTGGCTCTCCCACATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCAGAGTCGCt                                                               <  1:939877/150‑1 (MQ=255)
                                                                                          gTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTgg                                                             <  2:1038610/150‑1 (MQ=255)
                                                                                               tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCg                                                        <  1:1580579/150‑1 (MQ=255)
                                                                                                ccAGTTCAAATGCAATTGCCTTCAATGCACCTTCTTAGCTGTGGTGAGCCATCGGTGCTTGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGa                                                       <  1:879372/150‑1 (MQ=255)
                                                                                                 cAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTAACAGTCGCTGGCATCGAg                                                      >  2:1476141/1‑150 (MQ=255)
                                                                                                     tCAAATGCAATTGCCTTCAATGCACCTTCGTAGGTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACg                                                  <  1:252139/150‑1 (MQ=255)
                                                                                                      aaaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACgg                                                 <  1:1939682/149‑1 (MQ=255)
                                                                                                       aaaTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCATCGGTGCTGGCTCTCCCCCATTCACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGa                                                <  1:667455/150‑1 (MQ=255)
                                                                                                            cAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACt                                           <  2:567304/150‑1 (MQ=255)
                                                                                                                 gCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTACCCCATTTACGGTTAAGAATTCATTTTACGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTGGCTGGCATCGAGCACGGAAAACTTAATc                                      <  2:1641398/150‑1 (MQ=255)
                                                                                                                  ccTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCg                                     <  1:1730152/150‑1 (MQ=255)
                                                                                                                    ttCAATGCACCATCGTAGCTGTGGTGAGCCAGCGGAGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAAAACCGTCGGCAATACCTGACATAAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGaa                                   <  1:1189895/150‑1 (MQ=255)
                                                                                                                            aCCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAcc                           <  1:1227448/150‑1 (MQ=255)
                                                                                                                                  gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                     <  1:1933892/150‑1 (MQ=255)
                                                                                                                                  gTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGtt                     <  1:1423505/150‑1 (MQ=255)
                                                                                                                                   tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGGTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAAACCTGACATTAATACGTCACAGTGGCTGGCATCCCGCACGGAAAACTTAATCGAAGACGCACCACCGTta                    >  2:1418957/1‑150 (MQ=255)
                                                                                                                                   tAGCTGTGGTGAGCCAGCGGTGCGGGCTCTACCCCAATTACGGTTAAGAAATCATTTTACGAGTTAATAACCGACGCAATAACTGACATTATTAAGTCACAGTAGCAGGCCAGTCACACGGAAAACATAAACGAACACGAACACCAGTta                    >  2:155088/1‑150 (MQ=255)
                                                                                                                                      cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaata                 <  1:1305596/150‑1 (MQ=255)
                                                                                                                                        ggggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAc               >  1:995236/6‑150 (MQ=255)
                                                                                                                                         tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAAcc              <  2:407684/150‑1 (MQ=255)
                                                                                                                                         gggagAGCCAGCACCGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGATCCACAGTTAATAAcc              >  1:543791/5‑150 (MQ=255)
                                                                                                                                          ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa             >  1:1073170/1‑150 (MQ=255)
                                                                                                                                          ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCa             >  1:1223802/1‑150 (MQ=255)
                                                                                                                                          ggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTGAATAACCa             >  1:641287/1‑150 (MQ=255)
                                                                                                                                           gtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACATGACATTAATACATCACAGTCGCTGGCATCGAGCACGGAAAACATAATCGAAGACGAACCACAGTAAATAACCaa            >  2:1705078/1‑150 (MQ=255)
                                                                                                                                             gAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaaa          <  2:1679937/150‑1 (MQ=255)
                                                                                                                                              aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaac         <  1:1640587/150‑1 (MQ=255)
                                                                                                                                               gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGCCGAACCACAGTTAATAACCAAaaca        >  2:1256420/1‑150 (MQ=255)
                                                                                                                                               gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaaca        <  1:694132/150‑1 (MQ=255)
                                                                                                                                                 cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAAAACCTGACATTACTACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGCAAAACAGTTAAATACCAAaacaac      >  1:86654/1‑150 (MQ=255)
                                                                                                                                                 cAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCAGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTTATCGAAGACGAACCACAGTTAATAACCAAaacaac      >  2:824144/1‑150 (MQ=255)
                                                                                                                                                   gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg    <  1:644724/150‑1 (MQ=255)
                                                                                                                                                   gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGAAATACCTGACATTAATACTTCACAGGCGCTGGCATCGAGCACGGAATACTTAATCGAAGACGATCCACAGTTATTACCCAAAACAACCg    <  2:1668323/150‑1 (MQ=255)
                                                                                                                                                     ggTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGa  >  1:1259245/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGA  >  NC_000913/3263351‑3263649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: