Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,109 A→T 6.5% K766* (AAA→TAA)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1090AT 6.5% 344.7 / 6.2 129K766* (AAA→TAA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (62/57);  new base T (6/3);  total (69/60)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

CTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGT  >  NC_000913/3290961‑3291258
                                                                                                                                                    |                                                                                                                                                     
   ggTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAAcc                                                                                                                                                   <  2:312658/150‑1 (MQ=255)
       gATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGGGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCACCGCCAATATCGGTTATGGCGTACTTGTTACAGTCAGCGATCGCAACGCTAAACCGTTg                                                                                                                                               >  1:1492860/1‑150 (MQ=255)
        aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGCTCGCAACGGTAAACCGTTGc                                                                                                                                              >  1:626601/1‑150 (MQ=255)
         tactacGCAGCTGCCCGATAACGTCGGTCGTGATCAAACAACACAGTTTGTGGTGCCCCCCAGGGGGGCAGTGGTTGCGGCGCGGTTCAGCGCGCTGGTGGGTTGTCGCGGACTTGTTAGAGGCTGCGATCGCAACGGTGACCCGTTggg                                                                                                                                             >  2:155307/1‑148 (MQ=255)
         tactacGCAGCTGCCCGATAACGTCGATCTTGAACATACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCGAATATCGGTTATCGCGTAGTTGTTACAGTCAGCGATCGCAACGGTTAGCCGTTGcc                                                                                                                                             >  2:175131/1‑150 (MQ=255)
           ctgcGCAGGTGCCGGATGGCGTCGATCGTGACCAAACACCACAGTTTGTGGTGCGCAACGGAGGTGCAATGGTAGCGGCGCGTGTCCACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTAAGCGTTGCCCt                                                                                                                                           >  2:1219268/4‑150 (MQ=255)
            tacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatc                                                                                                                                          <  1:1253057/150‑5 (MQ=255)
             acGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGTGATCGCAACGGTAAACCGTTGCCCttt                                                                                                                                         <  1:761915/150‑1 (MQ=255)
              cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                        >  1:510107/1‑150 (MQ=255)
                   cTGCCCGGTAACGTCGATGTTGAACCAACAAGCCTGTTTGTGGGGACCAACCGAGGGGCAATGGTAGCGGCGCGTTTCAACGCCATTATCGGTTATCGGGGTCTTGTTAGAGTCAGCGAACGCAACGGGAACCCGTGGCCCTTTGGCGct                                                                                                                                   >  2:1456330/1‑150 (MQ=255)
                   cTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCCACGCCAATATCGGTTATCGCGTACTTGTTACAGTCTGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGct                                                                                                                                   >  2:118024/1‑150 (MQ=255)
                   cTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGct                                                                                                                                   >  1:2031719/1‑150 (MQ=255)
                     gCCCGAAAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAAAGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTTCAGTCAGCGCTCGCAACGGTAAACCGATGCCCATAGGCGctct                                                                                                                                 >  1:792885/1‑150 (MQ=255)
                      cccGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCtt                                                                                                                                <  1:1881123/150‑1 (MQ=255)
                       ccGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTg                                                                                                                               <  2:1343024/150‑1 (MQ=255)
                        cGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGGCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGc                                                                                                                              <  1:1495626/150‑1 (MQ=255)
                         gATACCGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGcc                                                                                                                             <  1:1184193/150‑1 (MQ=255)
                         gATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCACAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGcc                                                                                                                             <  2:284483/150‑1 (MQ=255)
                             aCGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCa                                                                                                                         <  2:1960148/150‑1 (MQ=255)
                             aCGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCa                                                                                                                         <  1:1667180/150‑1 (MQ=255)
                              cGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCaa                                                                                                                        >  1:1981416/1‑150 (MQ=255)
                              cGTCGATCTTGAACAAACAACACAGTGTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCaa                                                                                                                        >  1:987286/1‑150 (MQ=255)
                               gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                                                                       <  1:1409092/150‑1 (MQ=255)
                               gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAAc                                                                                                                       >  1:856652/1‑150 (MQ=255)
                                   aTCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                                                                   <  2:1610684/150‑1 (MQ=255)
                                   aTCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                                                                   <  1:753300/150‑1 (MQ=255)
                                     cTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatgat                                                                                                                 <  1:1462639/150‑1 (MQ=255)
                                      ttGTACATACAACACAGTTTGTGGTGCCCAACAGAGGATCAATTGTAGCGGCGCGTTTCAACTCCAATTTCGGTTATCGCGTACTTGTTACAGTCTGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                                                                                <  1:348505/150‑1 (MQ=255)
                                      ttGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATa                                                                                                                <  1:938985/150‑1 (MQ=255)
                                       tGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCTGCACCGATGATAc                                                                                                               >  2:1111007/1‑150 (MQ=255)
                                       tGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATAc                                                                                                               >  1:96712/1‑150 (MQ=255)
                                        gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACg                                                                                                              >  1:593171/1‑150 (MQ=255)
                                             aacaacACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGca                                                                                                         <  2:383976/150‑1 (MQ=255)
                                                 acacAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACCGTCAGCGCTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGAAACGGGGcaacaa                                                                                                     >  1:191319/1‑150 (MQ=255)
                                                 acacAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaacaa                                                                                                     <  2:1993399/150‑1 (MQ=255)
                                                   ccaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAg                                                                                                   <  2:1575089/149‑1 (MQ=255)
                                                        ttGTGGTGCGCAACAGCGGAGGAATGGTAGCGTCCCTTTACAACGACTATTTAGGATTGCGCGTACTTGTAACTGGCCTTGTTGGGAACGGTAATCCGTTGCACGTGGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcg                                                                                              <  2:1553329/150‑1 (MQ=255)
                                                                 ccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAgg                                                                                     >  1:1024150/1‑150 (MQ=255)
                                                                 ccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAgg                                                                                     >  2:763975/1‑150 (MQ=255)
                                                                  cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACTGTCAGCTATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCGTGCCAGCCACGCTGCGACGGGGCAACAAAGTATCGGCGATGCggg                                                                                    >  2:1008361/1‑150 (MQ=255)
                                                                  cAACAGAGGTGCAATGGGAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAggg                                                                                    <  1:166468/150‑1 (MQ=255)
                                                                   aaCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTAACGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggc                                                                                   <  2:1526192/150‑1 (MQ=255)
                                                                     cAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcgg                                                                                 >  2:149837/1‑150 (MQ=255)
                                                                      agagGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcggc                                                                                <  1:1223718/150‑1 (MQ=255)
                                                                      agagGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcggc                                                                                <  2:794821/150‑1 (MQ=255)
                                                                       gagGTGCAATGGTAGCGGCGCGTTTCCCCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCCACGATGATCCGGGGCAACAAAGTATCGTCGATGAGGGCGGCa                                                                               >  1:1833520/1‑150 (MQ=255)
                                                                           tGCTATGGTAGCGGCGCGTTTCAACGCCAATATCGTTTTTCGCGTACTTGTTACAGTCAGCGTTCGCTACGGTTAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGTTACGGGGCATCAAAGTATCGTCGATGAGGGCGGCATACt                                                                           <  2:478239/150‑1 (MQ=255)
                                                                           tGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACt                                                                           >  1:734942/1‑150 (MQ=255)
                                                                           tGCAATGGTAGCGGCGCGTTTCAAAGCCAATATCGGTTATCGCGTACTTGTTACAGTAAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACt                                                                           >  1:1343414/1‑150 (MQ=255)
                                                                            gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                          >  1:1072110/1‑150 (MQ=255)
                                                                            gCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                          >  1:787887/1‑150 (MQ=255)
                                                                             cAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                                                                         >  1:265681/1‑150 (MQ=255)
                                                                               aTGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTatat                                                                       >  1:278588/1‑150 (MQ=255)
                                                                                 ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtct                                                                     >  1:1109878/1‑150 (MQ=255)
                                                                                     gcggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATctctct                                                                 <  2:610988/150‑1 (MQ=255)
                                                                                       ggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCTGCGATCGCATCGGAAAACCGTTTCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                               <  2:575954/150‑1 (MQ=255)
                                                                                         cgcgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCCACGGTAAACCGTTGCCCTGTGGCGCTCGTGCCAGCAACGATGAGACGGGGCCACAAAGTAGCGTCGTTGAGGGCGGCCGTATATAGCTCTCggggt                                                             >  2:1843622/1‑149 (MQ=255)
                                                                                                cAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGTGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg                                                      <  2:939297/150‑1 (MQ=255)
                                                                                                 aaCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGt                                                     >  1:932666/1‑150 (MQ=255)
                                                                                                   cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                   <  2:1643104/150‑1 (MQ=255)
                                                                                                   cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                   >  2:709798/1‑150 (MQ=255)
                                                                                                    ggcAATATCGGTTATCGCGTACTTGTTACCGTCAGCGATCGCAACGGTTAACCGTTGCACTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAATGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGACTaaa                                                  >  1:20704/3‑150 (MQ=255)
                                                                                                     ccAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAAt                                                 >  2:848694/1‑150 (MQ=255)
                                                                                                      cAATATCGGTTCTCGGGTACTTGTTACAGACAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTCTCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATc                                                <  2:1389589/150‑1 (MQ=255)
                                                                                                      cAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATc                                                <  2:2049929/150‑1 (MQ=255)
                                                                                                       aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATcc                                               >  1:1900566/1‑149 (MQ=255)
                                                                                                             ggTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATAAAAAAGc                                         >  1:142790/1‑150 (MQ=255)
                                                                                                               gtcgcgcgTACTGCGTACACTCCGCGATCGCAACGGAAACCCGTTGCCCTTTGGCGCTCTTGCCAGCAACCAGCATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTg                                       <  2:2604/146‑1 (MQ=255)
                                                                                                                tATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCCACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTgg                                      >  2:1332691/1‑150 (MQ=255)
                                                                                                                   cgcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACt                                   >  2:1915514/1‑150 (MQ=255)
                                                                                                                    gcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTg                                  >  2:422916/1‑150 (MQ=255)
                                                                                                                      gttcTTGTTACAGACAGCGATCGCAACGGTAAACCGTTGCCCTGGGGCGCGCTGGCCAGCAACGATGATACGGGGCAAAAAAGTAACGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTa                                <  2:337470/147‑1 (MQ=255)
                                                                                                                      gTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAATTATCGTCGATGATGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTa                                <  1:1733435/150‑1 (MQ=255)
                                                                                                                         cTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGc                             >  1:1754035/1‑150 (MQ=255)
                                                                                                                            gTTCCAGCCCGCGATCCCAACGGTAAACCGTCGCCCTTTGGCGCTCTTGCCAGCAACGATGACACGGGGCAACAAAGTATCGTCGATGATGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTgg                          <  1:155307/150‑1 (MQ=255)
                                                                                                                            gTTACAGTCAGCGATCGCAACGGAAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTgg                          <  1:175131/150‑1 (MQ=255)
                                                                                                                                cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGCCTGGACGCTGGGGaa                      >  2:1353415/1‑150 (MQ=255)
                                                                                                                                 aGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGaaa                     <  2:1379055/150‑1 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                    >  1:2049067/1‑150 (MQ=255)
                                                                                                                                   tCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGTGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATc                   <  2:1482103/150‑1 (MQ=255)
                                                                                                                                   tCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATc                   <  2:265681/150‑1 (MQ=255)
                                                                                                                                    cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa                  >  2:848528/1‑150 (MQ=255)
                                                                                                                                    cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa                  >  1:1485956/1‑150 (MQ=255)
                                                                                                                                     cgcgcTCGCACCGGAACCCCGTCGCCCTATGGCGCCCATCCCAGCAACCACCAAACGGGCCAACAAATTATCCACGAAGAGGTCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg                 <  1:157712/149‑1 (MQ=255)
                                                                                                                                     aGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg                 >  1:538075/1‑150 (MQ=255)
                                                                                                                                     aGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg                 >  2:820347/1‑150 (MQ=255)
                                                                                                                                       cGATCTCATCGGTACACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACATTGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGc               <  1:839854/150‑1 (MQ=255)
                                                                                                                                       cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGc               <  2:1181225/150‑1 (MQ=255)
                                                                                                                                        gATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCa              <  2:1109877/150‑1 (MQ=255)
                                                                                                                                         aTCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAg             >  1:1127301/1‑150 (MQ=255)
                                                                                                                                           ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt           >  2:1752459/2‑150 (MQ=255)
                                                                                                                                           ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt           >  2:1112411/2‑150 (MQ=255)
                                                                                                                                             cAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATca         >  2:1799846/1‑150 (MQ=255)
                                                                                                                                              aaCGGTAAACCGTTGCCCTATGGCGCTCTCGCCAGCAACGATGATACGGGGCAACAAATTATCGTCGATGATGTCGGCATACTATATCTCTCTGGGATATTGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaa        <  1:1187501/150‑1 (MQ=255)
                                                                                                                                                cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaaca      >  1:1170239/1‑150 (MQ=255)
                                                                                                                                                 ggTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaacaa     >  1:590590/1‑150 (MQ=255)
                                                                                                                                                  gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAt    >  1:1607838/1‑150 (MQ=255)
                                                                                                                                                  gTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAt    >  2:1371998/1‑150 (MQ=255)
                                                                                                                                                   tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCAAACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATg   <  2:1024149/150‑1 (MQ=255)
                                                                                                                                                    aaaCCGTTGCCCTTTGGCGCTATTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCAGAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAAGGt  >  1:476444/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATGT  >  NC_000913/3290961‑3291258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: