Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,704 G→C 5.2% intergenic (+23/‑205) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,7040GC 5.2% 382.5 / 8.3 126intergenic (+23/‑205)ispB/sfsBoctaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (64/55);  new base C (5/2);  total (69/57)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 127 total aligned reads displayed.

CGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTG  >  NC_000913/3334560‑3334851
                                                                                                                                                |                                                                                                                                                   
   gTCAGCGTGCCGAGTAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCACCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCggg                                                                                                                                             <  1:1944916/150‑1 (MQ=255)
   gTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCggg                                                                                                                                             <  1:1167052/150‑1 (MQ=255)
    tCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGa                                                                                                                                            >  1:1097934/1‑150 (MQ=255)
    ccAGCGTGCCGAGGAAGAAGCAGACAAAGCCCTCGCAGCGTTACAGGAGCGCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACAGCGCTGTTCACTGCGATCGTTAATCCCCCCCCCTCACCCCGCGCACCGCGCGGGa                                                                                                                                            <  1:1343750/149‑1 (MQ=255)
      aGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATg                                                                                                                                          <  2:588888/150‑1 (MQ=255)
       gCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACGCCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATgg                                                                                                                                         >  1:240818/1‑150 (MQ=255)
       gCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCCCGCGCGCGGGGTgg                                                                                                                                         >  2:1744278/1‑150 (MQ=255)
        cGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGt                                                                                                                                        <  1:1566369/150‑1 (MQ=255)
          tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGATgaggg                                                                                                                                      <  1:577934/150‑5 (MQ=255)
          tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATgggtg                                                                                                                                      >  2:1984701/1‑147 (MQ=255)
           gCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGTTCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGGTgggtac                                                                                                                                     >  1:2044585/1‑146 (MQ=255)
           gCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTcc                                                                                                                                     <  2:139682/150‑1 (MQ=255)
              gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCCGt                                                                                                                                  >  2:1877104/1‑150 (MQ=255)
               aGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                                                                                 <  1:884404/150‑1 (MQ=255)
                ggAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGTTGGGTCCAGtta                                                                                                                                >  1:720683/1‑148 (MQ=255)
                ggAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTaa                                                                                                                                >  2:536584/1‑150 (MQ=255)
                 gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAg                                                                                                                               >  1:895072/1‑150 (MQ=255)
                    gcagCAGACAATGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCAGCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc                                                                                                                            <  2:348670/148‑1 (MQ=255)
                      agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                          >  1:48014/1‑150 (MQ=255)
                       gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACTTCGCTGTTCAACGCGATCGTTAATCCGCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCAt                                                                                                                         <  1:28450/150‑1 (MQ=255)
                        cAGTCATAGCCATCGCAGCGATACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                        <  1:355286/150‑1 (MQ=255)
                            cAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaaaaa                                                                                                                    >  2:260397/1‑150 (MQ=255)
                             aaaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACTCTATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAac                                                                                                                   <  1:528896/150‑1 (MQ=255)
                              aaGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAaca                                                                                                                  <  2:460010/150‑1 (MQ=255)
                               aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                 <  1:1820776/150‑1 (MQ=255)
                               aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                 <  1:1107331/150‑1 (MQ=255)
                                gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAACACtt                                                                                                               <  1:680232/150‑1 (MQ=255)
                                 ccATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACtt                                                                                                               >  1:1449378/1‑150 (MQ=255)
                                  cATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGAGGGTTCCAGTAAGTTCCATAAAAACACTTa                                                                                                              >  1:1333731/1‑150 (MQ=255)
                                     cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGGTCCAGGAAGTTCCATAAAAACACGTATTc                                                                                                           >  2:1992796/1‑150 (MQ=255)
                                     cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGAGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGGTCCAGGAAGTTCCATAAAAACCCTTAtta                                                                                                           >  2:1074744/1‑149 (MQ=255)
                                      gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa                                                                                                          >  1:1604003/1‑150 (MQ=255)
                                      gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGGTCCAGTAAGTTCCATAAAAACACTTAttaa                                                                                                          >  2:1654922/1‑148 (MQ=255)
                                       cAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAg                                                                                                         <  1:1190636/150‑1 (MQ=255)
                                         gCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTGAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGTTGGTTCCAGTAAGGTCCAGAAAAACACTTAtaaagag                                                                                                       >  2:352737/1‑144 (MQ=255)
                                          cGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGctc                                                                                                      <  1:811903/150‑1 (MQ=255)
                                           gTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGGCGCGCGGGAGGGTTGCAGGATGGTCCATTAAGACACTTATTGCGctct                                                                                                     >  1:148717/1‑150 (MQ=255)
                                            ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCCCGCGCGGGATGGTTCCAGTAGTTCCATAAAAACACTTATTAACCTCtat                                                                                                   >  2:655215/1‑150 (MQ=255)
                                            ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCta                                                                                                    <  1:1373481/150‑1 (MQ=255)
                                            ttACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCta                                                                                                    <  2:1984134/150‑1 (MQ=255)
                                              aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATGCCGCGCCGCGCGCGGGGTGGTTGCCGTAAGTTCCATAAAAACACTTATTGAGCTCgata                                                                                                  >  2:1571147/1‑150 (MQ=255)
                                              aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtata                                                                                                  >  2:449621/1‑150 (MQ=255)
                                              aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCCCGCAGCGCGCGGGATGGTTCCAGTAAGTTACATAAAAACACTTATTCAGCTCtata                                                                                                  >  2:423985/1‑150 (MQ=255)
                                              aCAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCCCGCAGCGCGCGGGATGGGTGCAGGAAGTTCCATAAAAACACTTTTTCAGCGCttta                                                                                                  >  1:1772287/1‑150 (MQ=255)
                                               cAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGGTGGTTGCAGTAAGTTACATAAAAACACATATTCAGCTCTaaaa                                                                                                 >  2:1868663/1‑150 (MQ=255)
                                               cAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTGCATAAAAACACTTATTCATCTCAATaa                                                                                                 >  2:1737014/1‑150 (MQ=255)
                                                 ggTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAACCACTTATTCAGCTCTTTAaca                                                                                               >  2:568492/1‑150 (MQ=255)
                                                 ggTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAaca                                                                                               <  1:1473788/150‑1 (MQ=255)
                                                   tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACAcc                                                                                             <  1:1080532/150‑1 (MQ=255)
                                                    gCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCCGGATGGTTCAAGTAAGTTCCATAAAAACACTTATTCAGCGCGATAacgacg                                                                                            >  1:1012474/1‑146 (MQ=255)
                                                     cTCCCGGCCACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGAGCCTTAATCCCCGCCCCTCATCCCGCGCAGCGCGCGGGATGGTGCCAGGAAGGTCCATAACAACACTTATTCAGCTCGCTAACGCCTg                                                                                           >  2:1239753/1‑150 (MQ=255)
                                                              acCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg                                                                                  <  2:628843/150‑1 (MQ=255)
                                                               cctgttGGGGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCTACGCGATCGTTAATCCCCTCCTCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACTCTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                 <  1:1528950/146‑1 (MQ=255)
                                                               ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                 >  2:1075984/1‑150 (MQ=255)
                                                               ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTACATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                 >  1:1950215/1‑150 (MQ=255)
                                                                  cTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtg                                                                              <  2:1496021/150‑1 (MQ=255)
                                                                     ggCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGc                                                                           <  2:1193462/150‑1 (MQ=255)
                                                                        gagaAGGACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGAGCGTTAATCCCCTCCCCTCATCCCCCGGAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                        <  2:1321044/150‑1 (MQ=255)
                                                                        gagaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                        >  1:3853/1‑150 (MQ=255)
                                                                          gacgCACTCATCGGCCTCGCGCTCATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGAGGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTATATAACACGTGAAAAATAGGGATGGGCATCtt                                                                      >  2:1316453/4‑150 (MQ=255)
                                                                            aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGGTCCAGTAAGTTCCATAAAAACACTTATTAAGCTCAATACCACCTGAAAACTAGGGATGTGCATCGTta                                                                    >  1:164874/1‑150 (MQ=255)
                                                                             gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtat                                                                   <  2:240818/150‑1 (MQ=255)
                                                                             gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtat                                                                   <  2:906007/150‑1 (MQ=255)
                                                                             gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtat                                                                   <  2:1121378/150‑1 (MQ=255)
                                                                               aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat                                                                 <  2:935674/150‑1 (MQ=255)
                                                                                   aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTc                                                             >  2:1109815/1‑150 (MQ=255)
                                                                                         cTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGATCCATAAAAACACTTAATCAGCTCTATAACAACTGAAAAATAGCGATGTGAATCTTTATATATTATGAatt                                                       >  2:1375152/1‑149 (MQ=255)
                                                                                           cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATAtt                                                     <  2:1043784/150‑1 (MQ=255)
                                                                                            gcgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTc                                                    <  1:1666326/150‑1 (MQ=255)
                                                                                             cgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTca                                                   >  1:1159257/1‑150 (MQ=255)
                                                                                              gcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcac                                                  <  2:1669078/150‑1 (MQ=255)
                                                                                               cacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTGCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTaaca                                                 >  1:2020359/1‑150 (MQ=255)
                                                                                                    ctcTGTTCAACGCGATCGTTAATGCCCTCCCCTCTTCCCTCTCAGAGCGAGGGATGGTTACAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCtt                                            <  1:1897397/148‑1 (MQ=255)
                                                                                                    cGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCACCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTATGAATATTCACAATCtt                                            >  2:120151/1‑150 (MQ=255)
                                                                                                       tGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCTTCTTTATATATTCTGAATATTCACACTCTTTAc                                         >  2:1053605/1‑150 (MQ=255)
                                                                                                        gTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACa                                        <  2:1519353/150‑1 (MQ=255)
                                                                                                        gTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACAATTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATATTTATATATTCTGAATATTCACACTCTTTACa                                        >  2:1379925/1‑150 (MQ=255)
                                                                                                         ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAg                                       <  2:149655/150‑1 (MQ=255)
                                                                                                           cAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGa                                     >  2:1885479/1‑150 (MQ=255)
                                                                                                           cAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGa                                     >  2:129431/1‑150 (MQ=255)
                                                                                                             aCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAc                                   <  1:449621/150‑1 (MQ=255)
                                                                                                              cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACt                                  <  2:1255401/150‑1 (MQ=255)
                                                                                                                   tCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa                             >  1:172241/1‑150 (MQ=255)
                                                                                                                    cGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTag                            <  2:596639/150‑1 (MQ=255)
                                                                                                                    cGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTag                            <  2:1145878/150‑1 (MQ=255)
                                                                                                                     gTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTGCAGTAAGTTACATAAAAACACTTATTGAGCTCTATAACACCTGAAAAATAGGGATGTGCATATTTTTATATTATGAATATTAACACTCTTTACAGGAACTTTTTata                           >  2:420861/1‑148 (MQ=255)
                                                                                                                      ttAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag                          <  2:824131/150‑1 (MQ=255)
                                                                                                                      ttAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag                          <  2:1323801/150‑1 (MQ=255)
                                                                                                                           cccctcccctCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATa                     <  1:1654923/150‑1 (MQ=255)
                                                                                                                             cctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTGACACTCTTTACAGGAACTTTTTAGAGCAATAgg                   >  1:877443/1‑150 (MQ=255)
                                                                                                                              ctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGc                  >  2:505553/1‑150 (MQ=255)
                                                                                                                              ctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGc                  <  2:714659/150‑1 (MQ=255)
                                                                                                                                  cctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATc              <  2:523445/150‑1 (MQ=255)
                                                                                                                                   accATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGTAAAATAGGGATGTGCATCTTTATATTTTCTGAATATTCACACTCTTTACAGGAACTTTTTAGATCAATAGGCCATCa             >  2:714518/3‑150 (MQ=255)
                                                                                                                                    ccATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg            >  1:1611278/2‑150 (MQ=255)
                                                                                                                                     cATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCGCACTCTTTACAGGATCTTTTTAGAGCAATAGGCCATCAgg           >  1:885451/1‑150 (MQ=255)
                                                                                                                                      aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa          >  1:1217838/1‑150 (MQ=255)
                                                                                                                                         ccGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGta       >  2:951344/1‑150 (MQ=255)
                                                                                                                                          cgcgCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAATTAGGGATGTGCATCTTGATTTATTCTGAATATTCACACTCTGTACAGGAACTTTTTAGAGCTATAGGCCATCAGGAGttt      >  2:1777536/1‑148 (MQ=255)
                                                                                                                                          cgcgCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGtat      >  1:1331142/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTG  >  NC_000913/3334560‑3334851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: