Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,982 G→C 6.3% intergenic (+28/+32) yhcG → / ← yhcH DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,9820GC 6.3% 386.7 / 12.5 124intergenic (+28/+32)yhcG/yhcHDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily
Reads supporting (aligned to +/- strand):  ref base G (67/49);  new base C (5/3);  total (72/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 126 total aligned reads displayed.

GGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGG  >  NC_000913/3368835‑3369130
                                                                                                                                                   |                                                                                                                                                    
ggTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                    >  2:39704/1‑150 (MQ=255)
ggTTTGCATATAACCTTTCTGTAAAGCGAATATATGATGCAACTACTTTATGAGAAACGACTCGCAGATTAACTCGTTCGAAGACAGGCGGTGCTAGAGGAATGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAgtg                                                                                                                                                    <  2:790375/150‑1 (MQ=255)
     tccTAACACCGTTCTGGCAAGCGAATATAAGATGGAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGc                                                                                                                                               <  2:1458646/149‑1 (MQ=255)
     gCCTACCCCCGCTCTCGCAAGCGAATCTAAGATGCAACTACCTGATGAGAACCGACTCGCAGATGAACTCGTCCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGGCTGc                                                                                                                                               <  2:253517/150‑1 (MQ=255)
     gCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGc                                                                                                                                               <  1:1636036/150‑1 (MQ=255)
      ccTAACTCCTTTCTGGCTAGCGAATATAAGATGCAACTACGTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTTTAGTCGCCGTTATTTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                              <  1:659985/150‑1 (MQ=255)
      ccTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCa                                                                                                                                              <  1:442147/150‑1 (MQ=255)
       cTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAg                                                                                                                                             >  2:831192/1‑150 (MQ=255)
        tAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGa                                                                                                                                            >  1:676432/1‑150 (MQ=255)
         aaCACCGTTCTGGCAAGCGAATATAAGATCCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGaa                                                                                                                                           <  1:236719/150‑1 (MQ=255)
           cacCGTTCTGGCAAGCGAATATAATATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCGATAGACGCCGGTAATTTAAGCGGTTTCGAATTATTCAGACAGTGTCTGCAGAAta                                                                                                                                         >  2:2004035/1‑149 (MQ=255)
            acCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCt                                                                                                                                        <  1:213508/150‑1 (MQ=255)
              cGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGATAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCttt                                                                                                                                      >  1:985596/1‑150 (MQ=255)
               gTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTa                                                                                                                                     <  2:12522/150‑1 (MQ=255)
               gTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTa                                                                                                                                     >  2:1739444/1‑150 (MQ=255)
                   tGGCAAGCTAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAAc                                                                                                                                 <  1:1495100/150‑1 (MQ=255)
                   tGGCAAGCGAATATAAGAAGCAACTACCTGATGAGAAACGACTCGCATATGAACTCGATCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTAAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTAAAAAc                                                                                                                                 >  2:1889003/1‑150 (MQ=255)
                    ggCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACg                                                                                                                                >  2:849401/1‑150 (MQ=255)
                     gCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGa                                                                                                                               >  1:794554/1‑150 (MQ=255)
                      cAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              >  2:610448/1‑150 (MQ=255)
                      cAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              >  1:1293296/1‑150 (MQ=255)
                      cAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGAAtt                                                                                                                                         <  2:2048483/139‑2 (MQ=255)
                      aaaGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGaa                                                                                                                              <  2:1689545/149‑1 (MQ=255)
                       aaGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAAc                                                                                                                             <  1:1193599/150‑1 (MQ=255)
                       aaGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGAGGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAAc                                                                                                                             <  1:1266866/150‑1 (MQ=255)
                          cGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTaa                                                                                                                          >  2:346758/1‑150 (MQ=255)
                            aaTATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGt                                                                                                                        <  1:1389952/150‑1 (MQ=255)
                               ataAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGtttt                                                                                                                     >  1:1827614/1‑150 (MQ=255)
                                taAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTa                                                                                                                    >  2:1791056/1‑150 (MQ=255)
                                 aaGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTaa                                                                                                                   >  2:636820/1‑150 (MQ=255)
                                   gATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGc                                                                                                                 >  2:189948/1‑150 (MQ=255)
                                     tGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGACGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTCCAGAATCTTTAAAACGATCTAAGTTTTAAGCCa                                                                                                               >  1:333078/1‑150 (MQ=255)
                                       cAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCAtt                                                                                                             >  2:586445/1‑150 (MQ=255)
                                         aCTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTaa                                                                                                           <  1:863125/150‑1 (MQ=255)
                                            aCCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATc                                                                                                        <  2:507449/150‑1 (MQ=255)
                                             ccTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACATGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCa                                                                                                       <  2:1470871/150‑1 (MQ=255)
                                              cTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCGGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTGAAACGAACTAAGTTTTAAGCCATTAAATCAg                                                                                                      >  1:1209801/1‑150 (MQ=255)
                                              cTGAGGAGAAACGACTCGCAGATTAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCTAATTCTGCAGACAGTGTCTGAAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAg                                                                                                      <  2:1876609/150‑1 (MQ=255)
                                                 atgaGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCATTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCt                                                                                                   >  1:1016307/1‑150 (MQ=255)
                                                    agaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTaa                                                                                                >  1:1012626/1‑150 (MQ=255)
                                                     gaAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAc                                                                                               >  2:1600946/1‑150 (MQ=255)
                                                      aaaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAAcc                                                                                              <  1:346758/150‑1 (MQ=255)
                                                       aaCGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAAAGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCt                                                                                             >  2:261241/1‑150 (MQ=255)
                                                          gACTCGCAGATGAACTCTTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTca                                                                                          <  1:1374833/150‑1 (MQ=255)
                                                           aCTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcac                                                                                         >  1:1844828/1‑150 (MQ=255)
                                                            ctctCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        <  1:1239841/150‑1 (MQ=255)
                                                            cTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  1:417389/1‑150 (MQ=255)
                                                            cTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        <  1:401463/150‑1 (MQ=255)
                                                            cTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTcacc                                                                                        >  1:956016/1‑150 (MQ=255)
                                                                 aGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAAc                                                                                   >  1:925079/1‑150 (MQ=255)
                                                                     gAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCttt                                                                               <  1:1890429/150‑1 (MQ=255)
                                                                        cTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTa                                                                            <  2:1404233/150‑1 (MQ=255)
                                                                          cGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGCCGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAt                                                                          >  2:1029072/1‑150 (MQ=255)
                                                                           gTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtc                                                                         <  1:636820/150‑1 (MQ=255)
                                                                               gAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAAtctctc                                                                     >  2:1305624/1‑150 (MQ=255)
                                                                               gAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTACAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCCCAACATTTTTAAtctctc                                                                     >  1:1204197/1‑150 (MQ=255)
                                                                                 acacAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCa                                                                   >  1:1655742/1‑150 (MQ=255)
                                                                                    caGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGc                                                                >  2:874305/1‑150 (MQ=255)
                                                                                      ggCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTc                                                              >  1:1473785/1‑150 (MQ=255)
                                                                                       gCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCg                                                             <  2:1605491/150‑1 (MQ=255)
                                                                                         ggTGCTAGAGGAATGCTATAGAAGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGcc                                                           <  1:1997544/150‑1 (MQ=255)
                                                                                              tAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACaa                                                      >  1:108929/1‑150 (MQ=255)
                                                                                               agagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAAc                                                     <  2:1460454/150‑1 (MQ=255)
                                                                                                gagGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACg                                                    >  1:1957193/1‑150 (MQ=255)
                                                                                                  ggAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCa                                                  >  2:127074/1‑150 (MQ=255)
                                                                                                  ggAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCAATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCa                                                  >  2:1247918/1‑150 (MQ=255)
                                                                                                    aaGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCTCAACCTTTTTAATCTCTCCAGCCTCGCCGACAACGCATc                                                >  2:2051820/1‑150 (MQ=255)
                                                                                                    aaGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATc                                                >  2:1792039/1‑150 (MQ=255)
                                                                                                      ggCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCt                                              <  2:417389/150‑1 (MQ=255)
                                                                                                       gCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTg                                             >  2:1588762/1‑150 (MQ=255)
                                                                                                        cTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTgg                                            >  1:330671/1‑150 (MQ=255)
                                                                                                        cTATAGACGCAGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGACTTAACCTTAACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTgg                                            >  2:605009/1‑150 (MQ=255)
                                                                                                          ataGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGtt                                          <  1:624721/150‑1 (MQ=255)
                                                                                                               cGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                     >  2:470191/1‑150 (MQ=255)
                                                                                                               cGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                     <  1:1541811/150‑1 (MQ=255)
                                                                                                               cGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGc                                     <  1:1739445/150‑1 (MQ=255)
                                                                                                                 ccGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACAAAAACCTTTTTAATCTATCCAGGCTCGCCGACAACGCATCCTGGTTTATGCgg                                   >  1:281883/1‑150 (MQ=255)
                                                                                                                  cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTTACCTTCACCACAACCTTTTTAATCTCTCCAGGATCGCAGAAAACGCATACTGGTTTTTTCGGt                                  >  2:1592020/1‑150 (MQ=255)
                                                                                                                  cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGt                                  <  2:489207/150‑1 (MQ=255)
                                                                                                                  cGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGt                                  <  1:1078481/150‑1 (MQ=255)
                                                                                                                    ttAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTc                                <  1:28041/150‑1 (MQ=255)
                                                                                                                       aTTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCAcc                             <  1:7462/150‑1 (MQ=255)
                                                                                                                         ttCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTg                           >  2:975504/1‑150 (MQ=255)
                                                                                                                         ttCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTg                           >  1:463148/1‑150 (MQ=255)
                                                                                                                            cgaggTTGCGAATTCTGCAGCCAGTGTCTGCAGAATCTTAACAACGAACTAAGTTTTAAGCCATTAAATCAGCCTGAACCTTCACCACCACCTGTTTAATCTCTCCAGGCGCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCa                        <  1:325055/147‑1 (MQ=255)
                                                                                                                             gCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCaa                       >  2:457426/1‑149 (MQ=255)
                                                                                                                                 tttCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCCTAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAac                   <  1:1444652/150‑1 (MQ=255)
                                                                                                                                    cGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACg                >  2:567611/1‑150 (MQ=255)
                                                                                                                                    cGAATTCTGCAGACAGTGTCTGCAGAAACTTTAAAACGAACAAAGTTTTAAGCCAATAAATCACCCTTAACCTTCACCACAACCTTATTAATCTCTCCAGGCTCGCCGACAACGCATCCTTGTTAAAGCGGAACACCTGGCATAAACACg                >  2:779877/1‑150 (MQ=255)
                                                                                                                                     gAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACgg               <  1:792997/150‑1 (MQ=255)
                                                                                                                                     agaTTCTGCAGACACTGTCTGCAGCATCTTTAAATCGAACTAAGTTTTAAGCCATTAAATCAGACTTAAACTTCACCACAACATTTTTAATCTCTCCAGGCTCGACGACAACTCATCCTCGTTTATGCGGTTCACCTGGCATAAACACgg               >  1:86133/3‑150 (MQ=255)
                                                                                                                                       aTTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCg             >  2:480385/1‑150 (MQ=255)
                                                                                                                                       aTTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCg             <  1:84700/150‑1 (MQ=255)
                                                                                                                                        ttCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGa            >  1:1457069/1‑150 (MQ=255)
                                                                                                                                          cTGCAGACACTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGagc          >  2:328267/1‑148 (MQ=255)
                                                                                                                                            gCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTTGTTTATGCGGTTCACCTGGCATAAACACGGCGAACAt        >  2:1911819/1‑150 (MQ=255)
                                                                                                                                            gCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACAt        >  1:1815352/1‑150 (MQ=255)
                                                                                                                                               gaaaGTGTCTGCAGAATCTTTAAAACGCACTAAGTTTTAAGCAATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGCTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTcc     >  2:80939/4‑150 (MQ=255)
                                                                                                                                                aCAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTccc    <  1:1519456/150‑1 (MQ=255)
                                                                                                                                                  aGTGTCTGCAGAATCTTTAAAACGAACTAAGTTGTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGTCATAAACACGGCGAACATTCCCgg  <  1:86937/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGTTTGCCTAACACCGTTCTGGCAAGCGAATATAAGATGCAACTACCTGATGAGAAACGACTCGCAGATGAACTCGTTCGAACACAGGCGGTGCTAGAGGAAGGCTATAGACGCCGTTAATTTCAGCGGTTTCGAATTCTGCAGACAGTGTCTGCAGAATCTTTAAAACGAACTAAGTTTTAAGCCATTAAATCAGCCTTAACCTTCACCACAACCTTTTTAATCTCTCCAGGCTCGCCGACAACGCATCCTGGTTTATGCGGTTCACCTGGCATAAACACGGCGAACATTCCCGG  >  NC_000913/3368835‑3369130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: