Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,530,672 G→C 5.1% intergenic (+20/+43) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,6720GC 5.1% 462.8 / 15.3 159intergenic (+20/+43)hslO/yhgEheat shock protein Hsp33/DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (69/78);  new base C (4/4);  total (73/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-02

Only 100 of 171 total aligned reads displayed.

TAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTT  >  NC_000913/3530523‑3530821
                                                                                                                                                     |                                                                                                                                                     
 aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                      <  1:1170309/150‑1 (MQ=255)
 aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                      >  2:935490/1‑150 (MQ=255)
   cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCTCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                    >  2:834546/1‑150 (MQ=255)
   cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                    >  2:1994425/1‑150 (MQ=255)
      ccTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGctct                                                                                                                                                 >  1:585243/1‑150 (MQ=255)
         ggCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGcc                                                                                                                                              <  2:1818925/150‑1 (MQ=255)
          gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                             <  2:1815768/150‑1 (MQ=255)
          gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                             >  2:64467/1‑150 (MQ=255)
           cGGAAGATGTCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                            <  1:1405543/150‑1 (MQ=255)
           cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                            <  2:689113/150‑1 (MQ=255)
           cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                            <  1:1938261/150‑1 (MQ=255)
             gAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAt                                                                                                                                          <  2:1957663/150‑1 (MQ=255)
             gAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATAATGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAt                                                                                                                                          <  2:1511778/150‑1 (MQ=255)
                  tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTTCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                     <  2:1032230/150‑1 (MQ=255)
                   ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                    >  1:759200/1‑150 (MQ=255)
                   ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                    >  2:1630752/1‑150 (MQ=255)
                    gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                                   >  1:1393112/1‑150 (MQ=255)
                    gCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                                   >  1:336474/1‑150 (MQ=255)
                     cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGAc                                                                                                                                  <  1:551615/150‑1 (MQ=255)
                     cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGAc                                                                                                                                  <  1:1880531/150‑1 (MQ=255)
                      gAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACt                                                                                                                                 >  2:1135496/1‑150 (MQ=255)
                          ttGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATa                                                                                                                             <  1:1414142/150‑1 (MQ=255)
                           tGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAAAAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATac                                                                                                                            >  2:1343157/1‑150 (MQ=255)
                             aCATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATacac                                                                                                                          <  2:452242/150‑1 (MQ=255)
                             aCATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATacac                                                                                                                          <  2:1490804/150‑1 (MQ=255)
                              cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                         >  1:213474/1‑150 (MQ=255)
                              cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGAAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                         >  2:1899310/1‑150 (MQ=255)
                                    ttGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgc                                                                                                                   >  2:1410960/1‑150 (MQ=255)
                                    ttGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATgc                                                                                                                   <  1:732601/150‑1 (MQ=255)
                                       tgATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCt                                                                                                                <  1:770248/150‑1 (MQ=255)
                                         aTTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCttt                                                                                                              >  1:175995/1‑150 (MQ=255)
                                             cTGCGGTAACCCCTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCCACAACGCGTCTCCGGCAGATCCGCAAGGTCATTAATGAGTATCTCCCGCAGAGAGGTCTCTGCAGCATTTTACGAATAATACACCTCATGCGCTTTGTCa                                                                                                          >  1:1276110/1‑150 (MQ=255)
                                              tGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCAc                                                                                                         <  1:978663/150‑1 (MQ=255)
                                               gCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACt                                                                                                        <  1:852048/150‑1 (MQ=255)
                                                  gTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCttt                                                                                                              <  2:209390/141‑1 (MQ=255)
                                                  gTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCAt                                                                                                     >  2:1074066/1‑150 (MQ=255)
                                                      ccACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACCCCTGATGCGCGTTGTCACTCATTCAg                                                                                                 >  2:887256/1‑150 (MQ=255)
                                                          tATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTc                                                                                             <  1:1567524/150‑1 (MQ=255)
                                                           aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGTCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTca                                                                                            >  2:463686/1‑150 (MQ=255)
                                                           aTCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTAATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGGTTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTca                                                                                            >  1:1285741/1‑150 (MQ=255)
                                                                ttCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTc                                                                                       <  2:1603089/150‑1 (MQ=255)
                                                                ttCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTc                                                                                       <  2:243021/150‑1 (MQ=255)
                                                                 tCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCGGGCTCTGCCGGCTTTTACTACTAATCCGCCTGGTGAGCTTTGTCAGTCATTCAGATTCACATTCa                                                                                      >  2:337922/1‑150 (MQ=255)
                                                                 tCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCa                                                                                      <  1:1213586/150‑1 (MQ=255)
                                                                  cAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAg                                                                                     >  2:1458086/1‑150 (MQ=255)
                                                                   aaTGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGGGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGc                                                                                    >  1:425461/1‑150 (MQ=255)
                                                                      gCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGATGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCtt                                                                                 <  2:1443216/150‑1 (MQ=255)
                                                                       cGATGGATATTGCTGAAATCCTCAACAACGCGTCGCCGTCAGATCCGCAAGTTCATTAATGAGTTTGGCCGGCAGAGAGGGCTCTGCCGTATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCttt                                                                                <  1:405202/150‑1 (MQ=255)
                                                                       cGATGGATATTGCTGAAATCCGCAACAACGCGACTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTATCGACTAATCCACCTGATGCGCTTTGTCACTCATTAAGATTCACATTCCCCCttt                                                                                >  2:1309346/1‑150 (MQ=255)
                                                                          tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                             <  2:41182/150‑1 (MQ=255)
                                                                          tGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAc                                                                             <  1:503055/150‑1 (MQ=255)
                                                                            gATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCa                                                                           <  2:1393111/150‑1 (MQ=255)
                                                                            gATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCa                                                                           <  1:631142/150‑1 (MQ=255)
                                                                                     gAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACa                                                                  <  1:536731/150‑1 (MQ=255)
                                                                                       aaTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAg                                                                >  2:1914799/1‑150 (MQ=255)
                                                                                         tCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCCCTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAt                                                              >  2:9403/1‑150 (MQ=255)
                                                                                          ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGCTGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATc                                                             >  2:203766/1‑150 (MQ=255)
                                                                                          ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATc                                                             >  2:1933451/1‑150 (MQ=255)
                                                                                           cGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcg                                                            >  2:542874/1‑150 (MQ=255)
                                                                                            gCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcgc                                                           <  1:1841776/150‑1 (MQ=255)
                                                                                             caacaaCGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcgcg                                                          <  1:712819/150‑1 (MQ=255)
                                                                                               acaacGCGTCTCCGGCAGCTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGCCTAATACACCTGATGCGCTGTGTCACTCATTCAGATTCACAGTCCGCCTTTCACCATCCACTACAAGATCGCGcc                                                        >  2:1828902/1‑150 (MQ=255)
                                                                                                  acGCGTCTCCGGCAGATCCTCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACg                                                     <  2:694649/150‑1 (MQ=255)
                                                                                                   cgcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCCTCCACTACAAGATCGCGCCACGc                                                    >  1:894336/1‑150 (MQ=255)
                                                                                                       tctcCGGCAGATCCGCAAGTTCCTTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCACCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTa                                                <  2:977453/150‑1 (MQ=255)
                                                                                                       tctcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCTCCACGCTTTa                                                <  2:1579246/150‑1 (MQ=255)
                                                                                                          ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGc                                             >  1:454062/1‑150 (MQ=255)
                                                                                                                 aTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcc                                      <  2:1447429/150‑1 (MQ=255)
                                                                                                                  tCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTccc                                     >  2:608725/1‑150 (MQ=255)
                                                                                                                   ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGCTCGCGCCACGCTTTAGGCTTCGTcccc                                    >  1:2043786/1‑150 (MQ=255)
                                                                                                                   ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                    >  2:1724445/1‑150 (MQ=255)
                                                                                                                   ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                    >  1:638962/1‑150 (MQ=255)
                                                                                                                    cGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCa                                   <  1:1057682/150‑1 (MQ=255)
                                                                                                                         gTTCATTAATGAGTAGGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCGTCGTCCCCAGtttt                              <  2:860087/150‑1 (MQ=255)
                                                                                                                          ttCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTc                             <  1:1723941/150‑1 (MQ=255)
                                                                                                                           tCATTAATGAGTATGTCCGGCAGAGATGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTcc                            <  1:1286482/150‑1 (MQ=255)
                                                                                                                           tCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTcc                            <  1:1478291/150‑1 (MQ=255)
                                                                                                                            cATTTATGAGTATGTCCGGCAGTGAGGGCTCTGCCGGATTTTACGTCTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCa                           <  1:1904801/150‑1 (MQ=255)
                                                                                                                            cATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCa                           <  2:336474/150‑1 (MQ=255)
                                                                                                                             aTTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACACGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCAt                          <  1:539467/150‑1 (MQ=255)
                                                                                                                               tAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATcc                        <  2:1644471/150‑1 (MQ=255)
                                                                                                                               tAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATcc                        <  2:1409927/150‑1 (MQ=255)
                                                                                                                                      taagTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTTATACCCCTGATGCGCTGTGTCACTCATTCAGATTCACATTCAGCCTTTCACAATCCACTACAAGATCGCGCCACGCTTTAGGCGTCGTCCCAAGTTTTCCATCCTTCGCAg                 >  1:241539/4‑150 (MQ=255)
                                                                                                                                      tATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAg                 <  1:945552/150‑1 (MQ=255)
                                                                                                                                         gTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtg              <  2:436331/150‑1 (MQ=255)
                                                                                                                                         gTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtg              >  1:904597/1‑150 (MQ=255)
                                                                                                                                          tCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATGCTTCGCAGCtgt             >  1:1012429/1‑150 (MQ=255)
                                                                                                                                           ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            >  1:154980/1‑150 (MQ=255)
                                                                                                                                           ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTGATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            <  2:1558895/150‑1 (MQ=255)
                                                                                                                                           ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            >  1:1607292/1‑150 (MQ=255)
                                                                                                                                           ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            <  2:585243/150‑1 (MQ=255)
                                                                                                                                           ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            <  2:812566/150‑1 (MQ=255)
                                                                                                                                           ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg            <  1:818252/150‑1 (MQ=255)
                                                                                                                                             ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg          <  1:1994426/150‑1 (MQ=255)
                                                                                                                                                 gagagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAt      >  2:123046/1‑150 (MQ=255)
                                                                                                                                                   gagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAttt    >  2:953455/1‑150 (MQ=255)
                                                                                                                                                    agGGCGCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAtttt   <  2:1129913/150‑1 (MQ=255)
                                                                                                                                                     gggCTCTGCCGGATTTTACGACTAATAGACCTTATGCTCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCAACTAGAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGATTAAttttt  <  1:1043096/150‑1 (MQ=255)
                                                                                                                                                     gggCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAttttt  <  2:768016/150‑1 (MQ=255)
                                                                                                                                                     gggCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAAttttt  >  2:1401790/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTT  >  NC_000913/3530523‑3530821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: