Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,610,544 T→C 5.5% H397R (CAC→CGC)  yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,610,5440TC 5.5% 328.5 / 6.6 109H397R (CAC→CGC) yhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (54/49);  new base C (3/3);  total (57/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 113 total aligned reads displayed.

ATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATAAA  >  NC_000913/3610396‑3610692
                                                                                                                                                    |                                                                                                                                                    
 ttCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGCCgtgt                                                                                                                                                    >  1:208673/1‑150 (MQ=255)
  tCTGGGTAACGTGGGTAATCAACTGCTGACCATGAAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACAACCGCAGAAAACATCATTAATACGCTGGATATTCAGTAAGTTTTAAGATGATGAGGCGGACTCAGGGACGTGtt                                                                                                                                                   >  1:1580303/1‑150 (MQ=255)
   cTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTc                                                                                                                                                  >  1:504478/1‑150 (MQ=255)
      ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCATCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                               <  1:1999425/150‑1 (MQ=255)
      ggTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCgg                                                                                                                                               >  1:1342453/1‑150 (MQ=255)
        tAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAg                                                                                                                                             <  1:647798/150‑1 (MQ=255)
           cGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACAGCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGcc                                                                                                                                          >  2:1430362/1‑150 (MQ=255)
            ttGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAACGCGCAGACCACCGCAGAAAACATCTTTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGCCg                                                                                                                                         <  2:1166168/149‑1 (MQ=255)
            gTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGCCg                                                                                                                                         <  1:1587629/150‑1 (MQ=255)
            gTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCg                                                                                                                                         >  1:742888/1‑150 (MQ=255)
               ggTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCCGGAACACGTCCCTGAGGCCgcct                                                                                                                                      <  2:529491/150‑4 (MQ=255)
                  aaTCAACTGCTGACCATGCAAATTACGCTGCCCGCAGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCCGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGtttttt                                                                                                                                   <  2:1968628/150‑1 (MQ=255)
                   aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                                  <  2:1626000/150‑1 (MQ=255)
                   aTCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                                  <  2:742887/150‑1 (MQ=255)
                   aTCAACTGCTGACCATGCAAATTACGCTGCCAGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGttttttt                                                                                                                                  <  2:1928019/150‑1 (MQ=255)
                     cAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTaa                                                                                                                                <  2:691560/150‑1 (MQ=255)
                       aCTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATc                                                                                                                              <  1:219935/150‑1 (MQ=255)
                         tgctgACCATTCAAATTACGCTGCCCGCTGACTATCAGCTAAAAGCGCCGACCACCGCAGCACCCAGCATTAATACGATTGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCATCAGTGACTTGTTCCGTAGGCCGTTTTTTTaagctc                                                                                                                            >  2:1341253/1‑146 (MQ=255)
                          gctgACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGcc                                                                                                                           <  1:1742574/150‑1 (MQ=255)
                             gACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACg                                                                                                                        <  1:1384331/150‑1 (MQ=255)
                               ccATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTc                                                                                                                      <  1:53878/150‑1 (MQ=255)
                                 aTGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATGCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCGACGTCAg                                                                                                                    >  2:100148/1‑150 (MQ=255)
                                   gCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACAACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGAGGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTGTTTTTAATCGCCACGTCAGta                                                                                                                  >  1:1355652/1‑150 (MQ=255)
                                         tACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGta                                                                                                                  >  2:1305466/1‑144 (MQ=255)
                                         tACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGta                                                                                                                  <  1:1305467/144‑1 (MQ=255)
                                         tACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAcg                                                                                                            >  1:916354/1‑150 (MQ=255)
                                          aCGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAcgc                                                                                                           >  2:953279/1‑150 (MQ=255)
                                           cGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAAcgcg                                                                                                          >  1:124011/1‑150 (MQ=255)
                                             cTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGAt                                                                                                        <  1:169759/150‑1 (MQ=255)
                                                cccGCTGACGAACAGCAAAAATCGCAGACCACCGCAGAAAACATCAATAAAACGCTGGTTATTCAGTAAGATTTAACATTATGACGCTGACTCAGGGACGTGTTCCGGAGGCCGTTTATTTAAACGACACGTCAGTAATAACGCGATTGc                                                                                                     >  2:1130433/1‑150 (MQ=255)
                                                  cGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGa                                                                                                   <  2:12089/150‑1 (MQ=255)
                                                   gCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAACCATCATTAATACGCTGGTTATTCAGTAAGGTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAc                                                                                                  <  1:106257/150‑1 (MQ=255)
                                                    cTGACGATCAGCAAAAAGCGTAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAcc                                                                                                 <  1:867325/150‑1 (MQ=255)
                                                    cTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAcc                                                                                                 <  2:1475997/150‑1 (MQ=255)
                                                    cTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGGTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGAcc                                                                                                 <  2:320018/150‑1 (MQ=255)
                                                        cGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTc                                                                                             <  1:1468715/150‑1 (MQ=255)
                                                          aTCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTccc                                                                                           >  2:659156/1‑150 (MQ=255)
                                                           tCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGCTGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccg                                                                                          <  2:1008019/150‑1 (MQ=255)
                                                              gCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccg                                                                                       >  2:1596961/1‑150 (MQ=255)
                                                               cAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGCCCAGTCccgccgc                                                                                      >  2:1527558/1‑150 (MQ=255)
                                                                aaaaaGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCccgccgcc                                                                                     >  1:639453/1‑150 (MQ=255)
                                                                aaaaaGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTGTTTAATCGCAACGTCACTAATAACGCGATTGCTACCAGTCccgccgcc                                                                                     >  1:146348/1‑150 (MQ=255)
                                                                      cgcAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAAACGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGa                                                                               >  1:231852/1‑150 (MQ=255)
                                                                       gcAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGAt                                                                              <  2:1471114/150‑1 (MQ=255)
                                                                       gcAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGAt                                                                              <  2:1342452/150‑1 (MQ=255)
                                                                        cAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCTGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGCCCAGTCCCGCCTCCGCCAGATa                                                                             >  2:480093/1‑150 (MQ=255)
                                                                             caccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCa                                                                        >  2:1932086/1‑150 (MQ=255)
                                                                              accGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAc                                                                       >  1:1852681/1‑150 (MQ=255)
                                                                              accGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAc                                                                       >  2:949610/1‑150 (MQ=255)
                                                                               cctcataaaaaaTAATTTATAAGCTTGTTAATCATTAAGTTTTAAGAATATGAGTCGTCCTCAGGGAAGTGTTCCTGATGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCTCCAGATAAATCAcc                                                                      <  2:1261876/144‑1 (MQ=255)
                                                                               ccGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCAcc                                                                      >  2:195053/1‑150 (MQ=255)
                                                                                 gCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTACTCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCgg                                                                    >  2:1988367/1‑150 (MQ=255)
                                                                                  cAGAAACCAGCATTAATAGGCTGGTTTTTCACTCCTTTTTAAGAGGATGACGCGGCCTCAGGGACGTGATCCGGAGGCCGGTTGTTTAATCGCCCCGTCAGTATTTTGGCGTTTGCGACCAGTCCCGCCGCCGCCAGTTAAATCACCGGt                                                                   <  2:187256/150‑1 (MQ=255)
                                                                                  cAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGt                                                                   <  1:1479087/150‑1 (MQ=255)
                                                                                   aGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTa                                                                  <  1:1947629/150‑1 (MQ=255)
                                                                                      aaaCATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTAAGGGACGTGTTCCGGAGACCGATTTTTTAATCTCCACGTCAGTAAAAACGCGATTGCGACCAGTCCCGACGCCGCCCGATAAATCACCGGTCcgc                                                               >  1:218360/1‑150 (MQ=255)
                                                                                         catcatTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgcccg                                                            >  1:1017207/1‑150 (MQ=255)
                                                                                           tcatTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTAcgcccgcc                                                          >  2:1580479/1‑150 (MQ=255)
                                                                                            catTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACgcccgccc                                                         <  2:1580428/150‑1 (MQ=255)
                                                                                            catTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACgcccgccc                                                         <  1:182321/150‑1 (MQ=255)
                                                                                             atTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTCCgcccgcccc                                                        >  1:789202/1‑149 (MQ=255)
                                                                                              ttAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAg                                                       >  2:402516/1‑150 (MQ=255)
                                                                                                 aTAAGCTGGTTATTCAGTAAGTCTTAAGATGATCAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCtc                                                    <  2:85683/150‑1 (MQ=255)
                                                                                                  tACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCtca                                                   <  1:1920949/150‑1 (MQ=255)
                                                                                                  aaCGCTGGTTATTCCGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCtca                                                   <  1:802766/149‑1 (MQ=255)
                                                                                                      cTGGATATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAACTCACCGGGACGCCCGCCCAGCTCATCAc                                               >  2:739140/1‑150 (MQ=255)
                                                                                                       tGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGTCAGATAAATCACCGGTACGCCCGCCCAGCTCCTCAcc                                              >  2:570690/1‑150 (MQ=255)
                                                                                                          ttATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagc                                           <  2:1121376/150‑1 (MQ=255)
                                                                                                           tATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagcc                                          <  1:923315/150‑1 (MQ=255)
                                                                                                           tATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCACGCCCAGCTCATCAccagcc                                          >  2:1500385/1‑150 (MQ=255)
                                                                                                               cAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGCGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagcccagc                                      >  2:216154/1‑150 (MQ=255)
                                                                                                               cAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCTCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagcccagc                                      >  2:1061175/1‑150 (MQ=255)
                                                                                                               cAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCCcccgccccgc                                      >  1:155730/1‑150 (MQ=255)
                                                                                                               cAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGACACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCAccagcccagc                                      >  2:620118/1‑150 (MQ=255)
                                                                                                                aGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACcagcccagcc                                     <  1:1824435/150‑1 (MQ=255)
                                                                                                                   aaGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGGTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGt                                  <  2:1948930/150‑1 (MQ=255)
                                                                                                                       tttAAGATGATGAGTCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGGCCCGCCGCCGCCAGATAACTCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTcc                             <  2:1979680/150‑1 (MQ=255)
                                                                                                                         tAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCGCATCACCAGCCCCGCCCGGGGTCCa                            >  1:967668/1‑150 (MQ=255)
                                                                                                                          aaGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAg                           <  1:683345/150‑1 (MQ=255)
                                                                                                                          aaGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAg                           >  1:686374/1‑150 (MQ=255)
                                                                                                                          aaGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCACGCCCCGCTCATCACCAGCCCAGCCAGTGGTCCAg                           >  2:1211109/1‑150 (MQ=255)
                                                                                                                           aGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAGCTCACCGGTCCGCCCGCCCCGCTCCTCACCCGCCCCGCCAGGGGTCCAGt                          >  1:165136/1‑150 (MQ=255)
                                                                                                                              tgatgaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGATTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACCCCCGCCCAGCTCATCACCAGCCCCGCCAGTGCTCCACTCAc                       >  2:31691/1‑150 (MQ=255)
                                                                                                                               aaaaaaacggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAAAAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGGCCAGTCCCg                      >  2:928010/8‑150 (MQ=255)
                                                                                                                                  gaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCCGCTCATCACCAGCCAAGCCAGTGGTCCAGTCCCGccc                   >  2:1012366/1‑149 (MQ=255)
                                                                                                                                  gaGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGccc                   >  2:1676170/1‑149 (MQ=255)
                                                                                                                                     gcggaCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCTGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCGCAGCCAGTGGTCCAGTCACGCCAAGc                <  2:1580302/150‑1 (MQ=255)
                                                                                                                                      cggcCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACTCCAAGCg               <  1:1061176/150‑1 (MQ=255)
                                                                                                                                       ggcCTCCGGAACACGTCCCTGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCGGCCGCCGCCAGATAAATCTCCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGa              >  1:765434/1‑150 (MQ=255)
                                                                                                                                        gcCTCAGGGACTTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGAt             <  2:208673/150‑1 (MQ=255)
                                                                                                                                        gcCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCCGTCACGCCAAGCGAt             >  1:877542/1‑150 (MQ=255)
                                                                                                                                         ccTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATa            >  2:1835687/1‑150 (MQ=255)
                                                                                                                                         ccTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATa            >  2:1733998/1‑150 (MQ=255)
                                                                                                                                           tCAGGGACGTGTTCCGGAGGCCGTTATTTAAATCGCCACGTCAGTAATAACGCGATTGCGAGCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCTGTGGTCCAGTCACGCCAAGCGATaaa          >  2:1048787/1‑150 (MQ=255)
                                                                                                                                            cAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTACAGTCACGCCAAGCGATAAAt         >  2:941890/1‑150 (MQ=255)
                                                                                                                                             cgggACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATc        <  2:1378459/149‑1 (MQ=255)
                                                                                                                                             aGGGACGTGTTCCGGAGGCCGTTTATTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATc        <  2:570324/150‑1 (MQ=255)
                                                                                                                                               ggACGTGGTCCGGAGGCCGTATTTTTAATCGCCACGTCAGTAATAACGCGAGTGCGACCAGTCCCGCCGCGGGCAGATAAAGCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCa      <  1:1294234/150‑1 (MQ=255)
                                                                                                                                                  cGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCCCCAGACCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATaa   >  2:610855/1‑150 (MQ=255)
                                                                                                                                                   gtgtTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATaaa  <  1:195053/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTCTGGGTAACGTGGGTAATCAACTGCTGACCATGCAAATTACGCTGCCCGCTGACGATCAGCAAAAAGCGCAGACCACCGCAGAAAACATCATTAATACGCTGGTTATTCAGTAAGTTTTAAGATGATGAGGCGGCCTCAGGGACGTGTTCCGGAGGCCGTTTTTTTAATCGCCACGTCAGTAATAACGCGATTGCGACCAGTCCCGCCGCCGCCAGATAAATCACCGGTACGCCCGCCCAGCTCATCACCAGCCCAGCCAGTGGTCCAGTCACGCCAAGCGATAAATCCATAAA  >  NC_000913/3610396‑3610692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: