Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,719,804 T→C 5.8% intergenic (+36/‑245) yiaG → / → cspA HTH_CROC1 family putative transcriptional regulator/RNA chaperone and antiterminator, cold‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,719,8040TC 5.8% 479.4 / 13.6 156intergenic (+36/‑245)yiaG/cspAHTH_CROC1 family putative transcriptional regulator/RNA chaperone and antiterminator, cold‑inducible
Reads supporting (aligned to +/- strand):  ref base T (67/80);  new base C (5/4);  total (72/84)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.75e-01

Only 100 of 165 total aligned reads displayed.

TCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTG  >  NC_000913/3719656‑3719953
                                                                                                                                                    |                                                                                                                                                     
gcGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTTAGTAAGGAGTTGATGGAATAGACTTTTATCCACTTTATTGATGTTTACGGTCCtgatg                                                                                                                                                      <  1:55424/149‑1 (MQ=255)
 cGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCtgatga                                                                                                                                                     >  2:173783/1‑150 (MQ=255)
 cGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCtgatga                                                                                                                                                     >  1:1439601/1‑150 (MQ=255)
       ggTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                                                                               >  1:779329/1‑150 (MQ=255)
        gTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                                                                              >  2:981006/1‑150 (MQ=255)
        gTAAAGGAATGGGAATCCAGACGCGTGAACCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                                                                              <  2:1372035/150‑1 (MQ=255)
         tAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCg                                                                                                                                             >  2:1478178/1‑150 (MQ=255)
          aaaGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGTCATCAGGACCGt                                                                                                                                            <  1:974307/150‑1 (MQ=255)
          aaaGGAATGGGAATCCAGACGCGTGAACCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGTCATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGTCATCAGGACCGt                                                                                                                                            <  2:37569/150‑1 (MQ=255)
             ggAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGtttt                                                                                                                                         >  1:1036821/1‑150 (MQ=255)
             ggAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGtttt                                                                                                                                         >  2:519101/1‑150 (MQ=255)
              gAATGGGAATCCAGACGCGTTAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGATTAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTc                                                                                                                                        <  1:1113049/150‑1 (MQ=255)
               aaTGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTcc                                                                                                                                       <  2:175119/150‑1 (MQ=255)
                aTGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATGAAGTAAGCAGGTGATGGAATAGACTTTTATCGACTTTATTGGTGTTTACGGTCGTGATGACAGGACCGTTTTcgc                                                                                                                                      >  2:1935421/1‑148 (MQ=255)
                  gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAAc                                                                                                                                    <  2:613420/150‑1 (MQ=255)
                   ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAAcc                                                                                                                                   >  1:1360484/1‑150 (MQ=255)
                        ccAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTa                                                                                                                              <  2:1076715/150‑1 (MQ=255)
                         cAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCTTTTTCCAACCGATTaa                                                                                                                             >  1:338274/1‑150 (MQ=255)
                         cAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTaa                                                                                                                             <  1:798235/150‑1 (MQ=255)
                          aGACGCGTGACGCCTTCAAGTGCCGAACAAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAAt                                                                                                                            <  2:169988/150‑1 (MQ=255)
                           gACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATc                                                                                                                           >  2:919217/1‑150 (MQ=255)
                           gACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATc                                                                                                                           <  1:1798462/150‑1 (MQ=255)
                           gACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATc                                                                                                                           >  1:626888/1‑150 (MQ=255)
                           gACGCGTGAACCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATc                                                                                                                           <  1:1259145/150‑1 (MQ=255)
                              gcgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATa                                                                                                                        <  2:1856168/150‑1 (MQ=255)
                               cgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATaa                                                                                                                       >  1:1512443/1‑150 (MQ=255)
                                 tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAat                                                                                                                     >  1:597165/1‑150 (MQ=255)
                                  gAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGCCAGGACCGTTTTCCAACCGATTAATCATAAata                                                                                                                    >  1:249738/1‑150 (MQ=255)
                                  gAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAATAAGTAAGCAGTTCATGGAATAGACTTTTATCCAGTATATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAata                                                                                                                    >  2:399441/1‑150 (MQ=255)
                                    aGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATg                                                                                                                  <  2:2006595/150‑1 (MQ=255)
                                       cTTCAAGTGCCGAAATAAAATTGATGCGTTTGATTAACGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGCCCGTTTTCCCACCGATTAATAAGAAATATGaaa                                                                                                               >  1:2044640/1‑150 (MQ=255)
                                        ttCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGaaaa                                                                                                              <  1:950482/150‑1 (MQ=255)
                                          cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAaat                                                                                                            <  1:1311753/150‑1 (MQ=255)
                                             gTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAaataat                                                                                                         >  1:132212/1‑150 (MQ=255)
                                                  gAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAttgttg                                                                                                    <  1:1188411/150‑1 (MQ=255)
                                                     cTAAAATTGATGCGTTTGATTCAAGCAAACCCGGCATTAAGAAAGAAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGATTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGAAt                                                                                                 >  2:1005098/1‑150 (MQ=255)
                                                       aaaaTTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCa                                                                                               <  1:616323/150‑1 (MQ=255)
                                                        aaaTTGATGCGGTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCAc                                                                                              <  2:1381430/150‑1 (MQ=255)
                                                          aTTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCTATTAATCATAAATATGAAAAATAATTTTTGCATCAccc                                                                                            >  1:282371/1‑150 (MQ=255)
                                                           ttGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCAcctg                                                                                           <  2:1642146/150‑3 (MQ=255)
                                                           ttGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCg                                                                                           <  2:859036/150‑1 (MQ=255)
                                                                gCGTTTGCTTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAAt                                                                                      >  1:380690/1‑150 (MQ=255)
                                                                 ggTTTGATTCAAGCCAACCCGGGATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATg                                                                                     <  2:806637/149‑1 (MQ=255)
                                                                  gTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGCCTTTTTTCCACTTTATTGCTGTTTACTGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATTAAAAATAATTTTTTCATCACCCGCCAATGc                                                                                    >  1:71704/1‑150 (MQ=255)
                                                                  gTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGc                                                                                    <  2:886005/150‑1 (MQ=255)
                                                                        ttCAAGCCAACCCGGCATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCt                                                                              <  2:1688870/150‑1 (MQ=255)
                                                                        ttCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAATAATAATTGTTGCATCACCCGCCAATGCGTGGCt                                                                              >  1:290624/1‑150 (MQ=255)
                                                                        ttCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCt                                                                              >  2:1131433/1‑150 (MQ=255)
                                                                         tCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCtt                                                                             <  2:1487719/150‑1 (MQ=255)
                                                                            aGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAAt                                                                          >  1:1381695/1‑150 (MQ=255)
                                                                              ccAACCCGGCATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGc                                                                        <  1:1987609/150‑1 (MQ=255)
                                                                              ccAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGc                                                                        <  2:1400875/150‑1 (MQ=255)
                                                                                aaCCCGGCATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCAAATGCGTGGCTTAATGcac                                                                      <  2:1009283/150‑1 (MQ=255)
                                                                                 aCCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGcaca                                                                     <  1:1633726/150‑1 (MQ=255)
                                                                                  cccGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACAt                                                                    >  1:850224/1‑150 (MQ=255)
                                                                                    cGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCa                                                                  <  2:817195/150‑1 (MQ=255)
                                                                                    cGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCa                                                                  <  1:1202988/150‑1 (MQ=255)
                                                                                         ttAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGt                                                             <  2:1153255/150‑1 (MQ=255)
                                                                                          taagtaagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGtt                                                            <  2:262742/150‑1 (MQ=255)
                                                                                             gtaagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTTCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGa                                                         <  2:1290328/150‑1 (MQ=255)
                                                                                             gtaagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGa                                                         <  2:779328/150‑1 (MQ=255)
                                                                                               aagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGCCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCCTCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGCCg                                                       >  2:847485/1‑150 (MQ=255)
                                                                                               aagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACg                                                       >  1:1302369/1‑150 (MQ=255)
                                                                                               aagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACg                                                       <  2:530162/150‑1 (MQ=255)
                                                                                                  cAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCATTGCGTGGCTTAATGCACATCAACGGTTTGACGTAc                                                    >  1:1113108/1‑150 (MQ=255)
                                                                                                   aGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACa                                                   >  1:1402632/1‑150 (MQ=255)
                                                                                                      tGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGAc                                                <  2:1548122/150‑1 (MQ=255)
                                                                                                      tGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGAc                                                >  1:654860/1‑150 (MQ=255)
                                                                                                       gATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGAcc                                               <  1:102143/150‑1 (MQ=255)
                                                                                                       gATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGAcc                                               <  2:170974/150‑1 (MQ=255)
                                                                                                        aTGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCa                                              <  2:1212825/150‑1 (MQ=255)
                                                                                                         tGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCAt                                             >  1:181616/1‑150 (MQ=255)
                                                                                                          ggAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCAGCAAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCAtt                                            >  1:1528974/1‑150 (MQ=255)
                                                                                                           gAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCTATTAATCATAATTATGAAAAATTATTGTTGCATCACCCGACAATGCGTGGCTTTATTCACATAAACGGTTTGACGTACATACCAtga                                           >  1:1776597/1‑148 (MQ=255)
                                                                                                            aaTATACTTTTATCCACTTTATTGATGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGACAATGCGTGGCTTAATGCACATCAACGGTTTGACTTACAGACCATTaa                                          <  1:454571/150‑1 (MQ=255)
                                                                                                            aaTAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATAACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTaa                                          <  2:338274/150‑1 (MQ=255)
                                                                                                               aGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCAAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGc                                       >  1:305796/1‑150 (MQ=255)
                                                                                                                gACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCa                                      >  1:538559/1‑150 (MQ=255)
                                                                                                                 aCTGTTCTCCACTTTATTGCTGTTTAAGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCCTCACCCGCCAATGCGTGGCTTAATGCACATCAACGGGTTGACGTACAGACCATTAAAGCAg                                     <  2:135838/150‑1 (MQ=255)
                                                                                                                  cTTTTATCCACTGTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGACAATGCGTGGCTTAATGCACATCAAAGGTTTGACGTACAGCCCTTTAcagcagg                                    >  2:1118862/1‑149 (MQ=255)
                                                                                                                   ttttATCCACTTTATTGCTGATTACGGTCCTGATGACAGGACCGTTTTCAAACCGATTACTCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAgtg                                   <  2:417756/150‑1 (MQ=255)
                                                                                                                    tttATCCACTTTATTGCTGTATACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAgtgt                                  <  1:1890860/150‑1 (MQ=255)
                                                                                                                        tCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAAAAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTgtagta                              >  2:1202883/1‑150 (MQ=255)
                                                                                                                         ccACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTaa                             >  2:730687/1‑150 (MQ=255)
                                                                                                                          cACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAGCCGATTAATCATAAATATTAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAg                            >  1:1984018/1‑150 (MQ=255)
                                                                                                                          cACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAg                            <  1:477430/150‑1 (MQ=255)
                                                                                                                             tttATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCa                         <  2:1360483/150‑1 (MQ=255)
                                                                                                                              ttATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCaa                        >  1:1764850/1‑150 (MQ=255)
                                                                                                                              ttATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCaa                        >  1:1756823/1‑150 (MQ=255)
                                                                                                                                  tGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTcc                    <  2:597165/150‑1 (MQ=255)
                                                                                                                                   gCTGTTTACGGTCCTGATGACAGGACCGTTTACCAACCGATTAATCATAAATATGAAAAATAATTGTTGCGTCACCCGCCAATGCGTGGCTTAATGGACAACATCGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTccc                   >  2:341463/1‑150 (MQ=255)
                                                                                                                                     ggaaaaCGGTCCTGTCATCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCtt                 >  1:770950/6‑150 (MQ=255)
                                                                                                                                      ttttACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTc                <  2:501423/149‑1 (MQ=255)
                                                                                                                                      gaaaaCGGTCCTGTCATCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTc                >  2:1584544/5‑150 (MQ=255)
                                                                                                                                         aaCGGTCCTGTCATCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAag             >  1:231447/2‑150 (MQ=255)
                                                                                                                                            ggTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGt          <  2:170031/150‑1 (MQ=255)
                                                                                                                                              tCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTa        <  1:984186/150‑1 (MQ=255)
                                                                                                                                                cTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATc      >  1:1830055/1‑150 (MQ=255)
                                                                                                                                                    tgaCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTg  <  1:1751187/150‑1 (MQ=255)
                                                                                                                                                    tgaCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGGGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTg  <  2:94197/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTG  >  NC_000913/3719656‑3719953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: