Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,640 T→A 6.5% intergenic (‑87/+21) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,6400TA 6.5% 324.3 / 9.8 118intergenic (‑87/+21)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (62/48);  new base A (3/5);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01

Only 100 of 119 total aligned reads displayed.

TTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCC  >  NC_000913/556491‑556787
                                                                                                                                                     |                                                                                                                                                   
tttCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGTCGTGCGTAATAACGGTTGGCATGATGGGCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGtt                                                                                                                                                     <  2:124224/150‑1 (MQ=255)
 ttCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTg                                                                                                                                                    >  1:621069/1‑150 (MQ=255)
  tCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGa                                                                                                                                                   >  2:1637238/1‑150 (MQ=255)
    gAACCCAACCCTTAACCAATGCATTGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGTTGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                 <  1:425420/150‑1 (MQ=255)
    gAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGGCGCTGCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                 <  1:1836477/150‑1 (MQ=255)
     aacccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATAc                                                                                                                                                <  2:1645780/150‑1 (MQ=255)
     aacccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATAc                                                                                                                                                >  1:1597194/1‑150 (MQ=255)
            cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCgg                                                                                                                                         <  1:1990312/150‑1 (MQ=255)
             ccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggt                                                                                                                                        <  1:573190/150‑2 (MQ=255)
              cTAAACCAATGCCTAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                       <  2:1772859/150‑1 (MQ=255)
              cTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCCATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                       >  1:922979/1‑150 (MQ=255)
               tAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggttc                                                                                                                                      <  2:1905588/150‑4 (MQ=255)
               aaaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggttc                                                                                                                                      <  2:511909/149‑4 (MQ=255)
                aaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGaaa                                                                                                                                     >  1:211879/1‑150 (MQ=255)
                aaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGaaa                                                                                                                                     >  1:1837246/1‑150 (MQ=255)
                 aaCCACTGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCAGCCTGTTGATACAGGCCGGGAAAg                                                                                                                                    <  2:1687471/150‑1 (MQ=255)
                 aaCCAATGCATAGTGTAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCTTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAg                                                                                                                                    <  1:391323/150‑1 (MQ=255)
                  aCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAgg                                                                                                                                   >  2:176135/1‑150 (MQ=255)
                      aTGCATAGGGGAACGGCGGCGGGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATc                                                                                                                               <  2:759695/150‑1 (MQ=255)
                       tGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCa                                                                                                                              >  1:254363/1‑150 (MQ=255)
                        gCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAg                                                                                                                             >  2:300248/1‑150 (MQ=255)
                          aTAGGGGAACGGCGGCGTGCGTAATAACGCTTGTCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGGCTGAAAAGAGAACCGGCCTGATGATACAGGCCGGGAAAGGGATCAGGc                                                                                                                           <  1:62575/150‑1 (MQ=255)
                               ggAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGcaacaa                                                                                                                      >  1:1297784/1‑150 (MQ=255)
                                       ggcgggcgTAAAAACGGTTGGCATGATGGTCGCTTCGGCAAAAAGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCGGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACg                                                                                                              <  1:1400792/150‑1 (MQ=255)
                                       ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACg                                                                                                              >  2:1199611/1‑150 (MQ=255)
                                       ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACg                                                                                                              >  1:255684/1‑150 (MQ=255)
                                         cgtgcgtAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCt                                                                                                            >  2:165915/1‑150 (MQ=255)
                                         cctgcgtAAAAACGGTTGGCATGATGGTAGCGTCGGCAAAAGGTCGATGCTATCAGCATGGCGGAACGGGGCGGAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCt                                                                                                            <  2:125456/148‑1 (MQ=255)
                                            gcgtAATAACGGGGGGCATGATGGTCGCTGCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg                                                                                                         <  2:1536276/150‑1 (MQ=255)
                                               taataaCGGTTGGCATGAAGGTCGCTTCGGCAAAATGTCGAGCCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGACTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGAcc                                                                                                      <  1:1349509/150‑1 (MQ=255)
                                                  taaCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGGTGATAAAGGCCGGGAAAGGGATCAGGCAACAACCTGTACCCTGTGACCTGc                                                                                                   >  2:1841559/1‑150 (MQ=255)
                                                         tGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTc                                                                                            <  1:555624/150‑1 (MQ=255)
                                                          ggCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCa                                                                                           >  1:401965/1‑150 (MQ=255)
                                                           gCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGTCGTAGAGTGCAAAAGTCTGAAACGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCAc                                                                                          <  2:1039431/150‑1 (MQ=255)
                                                            cATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACt                                                                                         >  1:1108906/1‑150 (MQ=255)
                                                            cATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACt                                                                                         <  1:28752/150‑1 (MQ=255)
                                                             atgaggGTCGCTTCGGCAAAATGGCGATTCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTg                                                                                        <  2:362406/150‑1 (MQ=255)
                                                              tgatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGt                                                                                       >  1:1124116/1‑150 (MQ=255)
                                                              tgatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGt                                                                                       >  2:962045/1‑150 (MQ=255)
                                                               gatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTc                                                                                      <  1:1264091/150‑1 (MQ=255)
                                                               gatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTc                                                                                      <  1:1051176/150‑1 (MQ=255)
                                                                ttgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGTATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCt                                                                                     <  1:1685799/149‑1 (MQ=255)
                                                                 ggggcGCTGCGTCAAAATGTCGATGCTATCAGCATGGATGATCGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGAGCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTg                                                                                    <  1:2028721/146‑1 (MQ=255)
                                                                   gTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGTCTGAc                                                                                  >  2:1435457/1‑150 (MQ=255)
                                                                   gTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGAc                                                                                  <  2:1761587/150‑1 (MQ=255)
                                                                           ggCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacg                                                                          >  1:1709337/1‑150 (MQ=255)
                                                                           ggCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacg                                                                          >  1:1209005/1‑150 (MQ=255)
                                                                            gCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacga                                                                         >  2:878542/1‑150 (MQ=255)
                                                                                  tGTCGATGCTATCAGCATGGATGACCGGGGCGTATAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACTACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                   <  1:1188574/150‑1 (MQ=255)
                                                                                  tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                   <  2:877434/150‑1 (MQ=255)
                                                                                  tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                   >  1:616906/1‑150 (MQ=255)
                                                                                  tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                   <  1:1101412/150‑1 (MQ=255)
                                                                                         gCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGttt                                                            >  1:1202051/1‑150 (MQ=255)
                                                                                          cTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGTAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGCACGGCGCCGATTTTGCAGCGTTTg                                                           >  1:363813/1‑150 (MQ=255)
                                                                                             tCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGAAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcgc                                                        >  2:445131/1‑150 (MQ=255)
                                                                                              cAGCATGGATGAACGGGGCGTAGAGGGCATAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGTCCGGCGACGATTTTGCAGCGTTTGcgcg                                                       <  1:262878/150‑1 (MQ=255)
                                                                                              cAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                       >  2:465852/1‑150 (MQ=255)
                                                                                              cAGAATGGATGAAAGGGGCGTAGAGGGCAAAAGTGTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                       <  1:303080/150‑1 (MQ=255)
                                                                                               aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGt                                                      >  1:453814/1‑150 (MQ=255)
                                                                                               aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGAAGCGTTTGCTCGt                                                      >  2:1095247/1‑150 (MQ=255)
                                                                                               aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCAGCAAAACTCACTGTCTGACCGGCGAAGATTTTGCAGCGTTTTCGCGt                                                      >  2:1557725/1‑150 (MQ=255)
                                                                                                 cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                    >  2:106511/1‑150 (MQ=255)
                                                                                                 cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                    <  1:958691/150‑1 (MQ=255)
                                                                                                 cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCAGCAAAACTCACTGTCTGACCCGCGACGATTTTGCCGGGTTTGCGCGttt                                                    >  2:1570764/1‑150 (MQ=255)
                                                                                                   tGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTcc                                                  >  1:1435677/1‑149 (MQ=255)
                                                                                                    ggATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCaa                                                 <  2:252111/150‑1 (MQ=255)
                                                                                                       tGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCg                                              <  1:438568/150‑1 (MQ=255)
                                                                                                       tGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCg                                              >  1:787514/1‑150 (MQ=255)
                                                                                                        gAACGGGGCGTATAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAATGGGATAAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGCCGATTTTGCAGCGTTTGCGCGTTTCAACCGc                                             >  2:1282326/1‑150 (MQ=255)
                                                                                                              ggCGTAGAGGGCAATAGGCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTc                                       <  2:1687038/150‑1 (MQ=255)
                                                                                                              ggCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTc                                       >  1:427087/1‑150 (MQ=255)
                                                                                                                  tAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACt                                   <  1:1887468/150‑1 (MQ=255)
                                                                                                                  tAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGAATTTGCAGCGTTTGCGCGTTTCAACCGCCCCGTCGagg                                   >  1:1968342/1‑148 (MQ=255)
                                                                                                                   agagGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACtt                                  <  1:772442/150‑1 (MQ=255)
                                                                                                                       ggCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAc                              >  1:1821174/1‑150 (MQ=255)
                                                                                                                        gCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAcc                             <  2:1297783/150‑1 (MQ=255)
                                                                                                                        gCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAcc                             <  1:1199612/150‑1 (MQ=255)
                                                                                                                            aactCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGc                         <  1:1638657/147‑1 (MQ=255)
                                                                                                                            aaGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGc                         <  2:1485225/150‑1 (MQ=255)
                                                                                                                              gTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCt                       <  1:1557726/150‑1 (MQ=255)
                                                                                                                                 tGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCg                    >  1:905370/1‑150 (MQ=255)
                                                                                                                                   aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                  <  1:221151/150‑1 (MQ=255)
                                                                                                                                   aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                  >  2:1713145/1‑150 (MQ=255)
                                                                                                                                   aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGACTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                  <  2:255684/150‑1 (MQ=255)
                                                                                                                                   aaaaGAGAACAGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                  <  1:1269479/150‑1 (MQ=255)
                                                                                                                                     aaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCaa                >  1:60560/1‑150 (MQ=255)
                                                                                                                                       gagaACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCCGCGTTTGCGCGTTTCAACCGCACCGTCGGCGTTCACCTGGCCGTCGGCAATc              >  2:1301428/1‑150 (MQ=255)
                                                                                                                                       gagaACCGGCCTGTTGAAACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCCCCGTCGACTTTCACCTGGCCTTCGGCAATc              >  2:858701/1‑150 (MQ=255)
                                                                                                                                        agaACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCATTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcg             >  2:2017309/1‑150 (MQ=255)
                                                                                                                                         gaACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcgc            >  2:249138/1‑150 (MQ=255)
                                                                                                                                         gaACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcgc            >  1:383223/1‑150 (MQ=255)
                                                                                                                                            ccGGCCTGTATCAACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt         >  1:538446/1‑150 (MQ=255)
                                                                                                                                            ccGGCCTGTATCAACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt         >  2:1188240/1‑150 (MQ=255)
                                                                                                                                              ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt       >  1:15137/1‑150 (MQ=255)
                                                                                                                                              ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt       <  1:1825180/150‑1 (MQ=255)
                                                                                                                                              ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt       >  2:1132865/1‑150 (MQ=255)
                                                                                                                                              ggCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt       >  1:1091021/1‑150 (MQ=255)
                                                                                                                                               gCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAtttt      >  1:1402250/1‑150 (MQ=255)
                                                                                                                                                  tGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc   >  1:46725/1‑150 (MQ=255)
                                                                                                                                                   gTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc  <  2:453814/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTTCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCC  >  NC_000913/556491‑556787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: