Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,719,806 A→T 5.5% intergenic (+38/‑243) yiaG → / → cspA HTH_CROC1 family putative transcriptional regulator/RNA chaperone and antiterminator, cold‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,719,8060AT 5.5% 461.4 / 9.6 156intergenic (+38/‑243)yiaG/cspAHTH_CROC1 family putative transcriptional regulator/RNA chaperone and antiterminator, cold‑inducible
Reads supporting (aligned to +/- strand):  ref base A (64/80);  new base T (5/4);  total (72/84)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 165 total aligned reads displayed.

CGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTGAT  >  NC_000913/3719657‑3719955
                                                                                                                                                     |                                                                                                                                                     
cGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCtgatga                                                                                                                                                       >  2:173783/1‑150 (MQ=255)
      tgTAAAGGAATGTGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAATATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                                                                                 <  1:653657/149‑1 (MQ=255)
      ggTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                                                                                 >  1:779329/1‑150 (MQ=255)
      ggTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAc                                                                                                                                                 >  1:1759011/1‑150 (MQ=255)
       gTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                                                                                >  2:981006/1‑150 (MQ=255)
       gTAAAGGAATGGGAATCCAGACGCGTGAACCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGAcc                                                                                                                                                <  2:1372035/150‑1 (MQ=255)
        tAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCg                                                                                                                                               >  2:1478178/1‑150 (MQ=255)
         aaaGGAATGGGAATCCAGACGCGTGAACCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGTCATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGTCATCAGGACCGt                                                                                                                                              <  2:37569/150‑1 (MQ=255)
           aGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGttt                                                                                                                                            <  1:1414430/150‑1 (MQ=255)
            ggAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGtttt                                                                                                                                           >  1:1036821/1‑150 (MQ=255)
            ggAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGtttt                                                                                                                                           >  2:519101/1‑150 (MQ=255)
             gAATGGGAATCCAGACGCGTTAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGATTAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTc                                                                                                                                          <  1:1113049/150‑1 (MQ=255)
              aaTGGGAATCCAGACGCGGGAAGCCTTGAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAGTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTcc                                                                                                                                         >  2:1061704/1‑150 (MQ=255)
               aTGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATGAAGTAAGCAGGTGATGGAATAGACTTTTATCGACTTTATTGGTGTTTACGGTCGTGATGACAGGACCGTTTTcgc                                                                                                                                        >  2:1935421/1‑148 (MQ=255)
                 gggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAAc                                                                                                                                      <  2:613420/150‑1 (MQ=255)
                   gAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCg                                                                                                                                    >  1:356751/1‑150 (MQ=255)
                     aTCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                                                                                  >  1:1364591/1‑150 (MQ=255)
                         aGACGCGTGACGCCTTCAAGTGCCGAACAAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAAt                                                                                                                              <  2:169988/150‑1 (MQ=255)
                          gACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATc                                                                                                                             >  2:919217/1‑150 (MQ=255)
                          gACGAGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGACTAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTACTc                                                                                                                             <  2:330337/150‑1 (MQ=255)
                              cgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATaa                                                                                                                         >  1:1512443/1‑150 (MQ=255)
                                tGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAat                                                                                                                       <  2:1708123/150‑1 (MQ=255)
                                 gAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGCCAGGACCGTTTTCCAACCGATTAATCATAAata                                                                                                                      >  1:249738/1‑150 (MQ=255)
                                 gAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCAATAAGTAAGCAGTTCATGGAATAGACTTTTATCCAGTATATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAata                                                                                                                      >  2:399441/1‑150 (MQ=255)
                                      cTTCAAGTGCCGAAATAAAATTGATGCGTTTGATTAACGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGCCCGTTTTCCCACCGATTAATAAGAAATATGaaa                                                                                                                 >  1:2044640/1‑150 (MQ=255)
                                       ttCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGaaaa                                                                                                                <  1:950482/150‑1 (MQ=255)
                                         cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAaat                                                                                                              <  1:1310268/150‑1 (MQ=255)
                                         cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAaat                                                                                                              <  1:1311753/150‑1 (MQ=255)
                                             tGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAtt                                                                                                          >  1:746730/1‑150 (MQ=255)
                                               ccGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAttgt                                                                                                        <  2:1512442/150‑1 (MQ=255)
                                                 gAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAAttgttg                                                                                                      <  1:1188411/150‑1 (MQ=255)
                                                  aaCTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGc                                                                                                     <  2:129276/150‑1 (MQ=255)
                                                   aCTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                                                                                    <  1:607373/150‑1 (MQ=255)
                                                   aCTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                                                                                    <  1:1768353/150‑1 (MQ=255)
                                                    cTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCAt                                                                                                   <  1:772726/150‑1 (MQ=255)
                                                    cTAAAATTGATGCGTTTGATTCAAGCAAACCCGGCATTAAGAAAGAAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGATTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGAAt                                                                                                   >  2:1005098/1‑150 (MQ=255)
                                                      aaaaTTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCa                                                                                                 <  1:616323/150‑1 (MQ=255)
                                                          ttGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCg                                                                                             <  2:859036/150‑1 (MQ=255)
                                                            gATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGTACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGcc                                                                                           <  1:993554/150‑1 (MQ=255)
                                                               gCGTTTGCTTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAAt                                                                                        >  1:380690/1‑150 (MQ=255)
                                                                ggTTTGATTCAAGCCAACCCGGGATTAAGTAAGCAGTTGATTGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATg                                                                                       <  2:806637/149‑1 (MQ=255)
                                                                 gTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGc                                                                                      <  2:886005/150‑1 (MQ=255)
                                                                    tGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTg                                                                                   >  2:1102197/1‑150 (MQ=255)
                                                                    tGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTg                                                                                   >  1:1545935/1‑150 (MQ=255)
                                                                     gATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTgg                                                                                  <  1:2046264/150‑1 (MQ=255)
                                                                       ttCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAATAATAATTGTTGCATCACCCGCCAATGCGTGGCt                                                                                >  1:290624/1‑150 (MQ=255)
                                                                        tCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCtt                                                                               <  2:1364590/150‑1 (MQ=255)
                                                                           aGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAAt                                                                            >  1:1381695/1‑150 (MQ=255)
                                                                             ccAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGc                                                                          <  1:285628/150‑1 (MQ=255)
                                                                                 cccGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGcacaa                                                                      >  1:592491/1‑149 (MQ=255)
                                                                                   cGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCa                                                                    <  1:1202988/150‑1 (MQ=255)
                                                                                   cGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCa                                                                    <  2:817195/150‑1 (MQ=255)
                                                                                     gCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAAc                                                                  <  2:1475347/150‑1 (MQ=255)
                                                                                     gCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAAc                                                                  <  2:1796723/150‑1 (MQ=255)
                                                                                        ttAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGt                                                               >  1:1955274/1‑150 (MQ=255)
                                                                                            gtaagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGa                                                           <  2:779328/150‑1 (MQ=255)
                                                                                             taagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGAc                                                          >  1:1356528/1‑150 (MQ=255)
                                                                                              aagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGCCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCCTCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGCCg                                                         >  2:847485/1‑150 (MQ=255)
                                                                                              aagCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACg                                                         <  2:530162/150‑1 (MQ=255)
                                                                                                 cAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATTAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTAc                                                      >  1:170031/1‑150 (MQ=255)
                                                                                                 cAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCATTGCGTGGCTTAATGCACATCAACGGTTTGACGTAc                                                      >  1:1113108/1‑150 (MQ=255)
                                                                                                 cAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTAc                                                      <  1:981007/150‑1 (MQ=255)
                                                                                                  aGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACa                                                     <  2:187096/150‑1 (MQ=255)
                                                                                                     tGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGAc                                                  >  1:654860/1‑150 (MQ=255)
                                                                                                      gATTGAATAGACTTTTATCCACTTTATTGCTGTTTCAGGTCCTGATGACAGGACCGTTTTACAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCATCGGTTTGACGTACAGAcc                                                 <  2:169439/150‑1 (MQ=255)
                                                                                                      gATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGAcc                                                 <  1:102143/150‑1 (MQ=255)
                                                                                                        tGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCAt                                               >  1:181616/1‑150 (MQ=255)
                                                                                                        tGGAATAGACTTTTATCAACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCAt                                               <  2:1225546/150‑1 (MQ=255)
                                                                                                          gAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCTATTAATCATAATTATGAAAAATTATTGTTGCATCACCCGACAATGCGTGGCTTTATTCACATAAACGGTTTGACGTACATACCAtga                                             >  1:1776597/1‑148 (MQ=255)
                                                                                                           aaTATACTTTTATCCACTTTATTGATGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGACAATGCGTGGCTTAATGCACATCAACGGTTTGACTTACAGACCATTaa                                            <  1:454571/150‑1 (MQ=255)
                                                                                                           aaTAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATAACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTaa                                            <  2:338274/150‑1 (MQ=255)
                                                                                                            aTAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTaaa                                           >  2:1680066/1‑150 (MQ=255)
                                                                                                                aCTGTTCTCCACTTTATTGCTGTTTAAGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCCTCACCCGCCAATGCGTGGCTTAATGCACATCAACGGGTTGACGTACAGACCATTAAAGCAg                                       <  2:135838/150‑1 (MQ=255)
                                                                                                                 cTTTTATCCACTGTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGACAATGCGTGGCTTAATGCACATCAAAGGTTTGACGTACAGCCCTTTAcagcagg                                      >  2:1118862/1‑149 (MQ=255)
                                                                                                                  ttttATCCACTTTATTGCTGATTACGGTCCTGATGACAGGACCGTTTTCAAACCGATTACTCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAgtg                                     <  2:417756/150‑1 (MQ=255)
                                                                                                                   tttATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAgtgt                                    <  1:873007/150‑1 (MQ=255)
                                                                                                                    ttATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGATCGTTTTCCAACCGATTTATCTTAAATATGAAAAATATTTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTgta                                   <  1:1478179/150‑1 (MQ=255)
                                                                                                                     tATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTgtag                                  <  1:187396/150‑1 (MQ=255)
                                                                                                                       tCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAAAAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTgtagta                                >  2:1202883/1‑150 (MQ=255)
                                                                                                                        ccACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTaa                               >  2:730687/1‑150 (MQ=255)
                                                                                                                         cACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAGCCGATTAATCATAAATATTAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAg                              >  1:1984018/1‑150 (MQ=255)
                                                                                                                         cACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAATTATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAg                              >  1:2010020/1‑150 (MQ=255)
                                                                                                                         cACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAg                              <  1:477430/150‑1 (MQ=255)
                                                                                                                         cACTGTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCACCCGAGTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGCCGGACAGACAATTAACGCAGTggagtacg                              >  2:1729185/1‑148 (MQ=255)
                                                                                                                           cTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCACCCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGc                            >  1:1289686/1‑150 (MQ=255)
                                                                                                                                 tGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTcc                      <  2:597165/150‑1 (MQ=255)
                                                                                                                                  gCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTccc                     >  2:1150483/1‑150 (MQ=255)
                                                                                                                                  gCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTccc                     >  2:1162689/1‑150 (MQ=255)
                                                                                                                                     ttttACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTc                  <  2:501423/149‑1 (MQ=255)
                                                                                                                                     gaaaaCGGTCCTGTCATCAGGCCCGTTTTCCAACCGATTAATCATAAATATGATAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTAGACGTACAGACCATTAAAGCAGTGTAGTCAGGCAAGGCCCttg                  >  1:307468/5‑149 (MQ=255)
                                                                                                                                     gaaaaCGGTCCTGTCATCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTc                  >  2:1584544/5‑150 (MQ=255)
                                                                                                                                      tttACGGTCCTGATGACAGGACCGTTTTGCAACCGATTAATCATAACTATGAAAAATAATTGTTGCATCACCAGCCAATGCGTGGCTGAATGCACATCAACGGTTTTACGTACAGACCATTAACGCAGTGTAGTAAGGCAAGTCCCTTCa                 <  2:1568670/150‑1 (MQ=255)
                                                                                                                                      tttACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCa                 <  1:519101/150‑1 (MQ=255)
                                                                                                                                         aCGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAaga              >  1:829920/1‑150 (MQ=255)
                                                                                                                                              ccTGATTACAGGAACGTTTTCGAACCGATTAATCATAAATATTAATATTAATTGTTGCATCACACGCCTCTGCGTGGCTTAATGCTCATCAACGGTTTGACGTAAAGACCATTAAAGCAGTGTAGTAATGCAAGTCCCTTAAAGAGTTAt         <  2:2044639/150‑1 (MQ=255)
                                                                                                                                               cTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATc        <  1:1815427/150‑1 (MQ=255)
                                                                                                                                                   tgaCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTg    <  2:1398054/150‑1 (MQ=255)
                                                                                                                                                   tgaCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTg    <  1:1751187/150‑1 (MQ=255)
                                                                                                                                                   tgaCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGGGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTg    <  2:94197/150‑1 (MQ=255)
                                                                                                                                                     aCAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTGAt  <  1:1220380/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTTTGACGTACAGACCATTAAAGCAGTGTAGTAAGGCAAGTCCCTTCAAGAGTTATCGTTGAT  >  NC_000913/3719657‑3719955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: