Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,741,091 G→T 5.3% intergenic (+133/+18) avtA → / ← ysaA valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,741,0910GT 5.3% 421.8 / 6.8 143intergenic (+133/+18)avtA/ysaAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase/putative hydrogenase, 4Fe‑4S ferredoxin‑type component
Reads supporting (aligned to +/- strand):  ref base G (68/66);  new base T (2/7);  total (70/73)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

GGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGC  >  NC_000913/3740942‑3741240
                                                                                                                                                     |                                                                                                                                                     
 ggCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTgaga                                                                                                                                                      >  1:1486526/1‑150 (MQ=255)
    tGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCAGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGaacc                                                                                                                                                   >  2:1507943/1‑148 (MQ=255)
      aaaGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGc                                                                                                                                                 >  1:80030/1‑150 (MQ=255)
        aGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCg                                                                                                                                               >  1:1162163/1‑150 (MQ=255)
        aGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCg                                                                                                                                               <  2:1355462/150‑1 (MQ=255)
         gTCACTAAAAATTGGCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGTATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                              <  1:1102036/150‑1 (MQ=18)
         gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                              <  1:1095088/150‑1 (MQ=255)
         gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCgg                                                                                                                                              <  2:550807/150‑1 (MQ=255)
         gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCgg                                                                                                                                              <  2:1541659/150‑1 (MQ=255)
         gTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCgg                                                                                                                                              <  1:1692748/150‑1 (MQ=255)
          tCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTTCTCAACGCCGGa                                                                                                                                             <  2:1937269/150‑1 (MQ=255)
            aCTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaa                                                                                                                                           >  1:829778/1‑150 (MQ=255)
             cTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGaaaa                                                                                                                                          >  1:936536/1‑150 (MQ=255)
               aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATTATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                        <  2:1837213/150‑1 (MQ=255)
               aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATTTATTGAATTTGGAATATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                        <  2:1889632/150‑1 (MQ=255)
               aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                        <  2:1964460/150‑1 (MQ=255)
               aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                        >  2:301379/1‑150 (MQ=255)
               aaaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACg                                                                                                                                        >  2:929386/1‑150 (MQ=255)
               aaaaaTTGCATGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGATTTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCCTCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAACCg                                                                                                                                        >  2:1020303/1‑150 (MQ=255)
                 aaaTTGCCTGTTGCGCTACGCTTTTCATGCCTTCATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGtt                                                                                                                                      <  2:1467985/150‑1 (MQ=255)
                     tGCCTGATGCGGTACGCTTATCAGGCCTACATGTTTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCaa                                                                                                                                  <  2:885030/150‑1 (MQ=255)
                     tGCCTGATGCGCTACGCTTATCAGGCCTACATTATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGAGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCaa                                                                                                                                  <  2:877608/150‑1 (MQ=255)
                        cTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACa                                                                                                                               >  1:1197578/1‑150 (MQ=255)
                         tGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAg                                                                                                                              >  2:1173579/1‑150 (MQ=255)
                           aTGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGgc                                                                                                                            <  1:404606/150‑1 (MQ=255)
                                 tACGCTTATCAGGTCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTTGCATAAACAAAGCGCACTGTTCCGGCGGTGAGAAACGCCGGAAAACGTTTCAAACAGGCtgctgc                                                                                                                      <  1:234172/150‑1 (MQ=255)
                                 tACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCtgctgc                                                                                                                      <  2:1715431/150‑1 (MQ=255)
                                   cGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGCAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCg                                                                                                                    >  1:2039533/1‑150 (MQ=255)
                                   cGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCg                                                                                                                    >  1:1400154/1‑150 (MQ=255)
                                    gCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGt                                                                                                                   >  2:652682/1‑150 (MQ=255)
                                        aTCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGcc                                                                                                               <  2:713422/150‑1 (MQ=255)
                                         tCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGTCTGCTGCCGTAGCCg                                                                                                              >  2:490441/1‑150 (MQ=255)
                                             gCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGc                                                                                                          <  2:1712808/150‑1 (MQ=255)
                                             gCCTACATGATTTATGCAATATTTTGAATTTGGAATAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGTGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGc                                                                                                          <  1:1030799/150‑1 (MQ=255)
                                                  cATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTg                                                                                                     >  2:507581/1‑150 (MQ=255)
                                                  cATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGATGCTGCCGTAGCCGATGCCGCTg                                                                                                     >  2:747737/1‑150 (MQ=255)
                                                   aTGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATTCCGCTGc                                                                                                    >  2:67723/1‑150 (MQ=255)
                                                    tGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGcc                                                                                                   >  1:459477/1‑149 (MQ=255)
                                                       tttCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACtt                                                                                                >  2:930974/1‑150 (MQ=255)
                                                            tcAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCg                                                                                           <  2:1308011/149‑1 (MQ=255)
                                                            gCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCg                                                                                           <  2:1765290/150‑1 (MQ=255)
                                                             cAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGa                                                                                          <  1:1478277/150‑1 (MQ=255)
                                                              aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGTTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                         >  2:1445896/1‑150 (MQ=255)
                                                              aaTATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGAc                                                                                         >  2:801731/1‑150 (MQ=255)
                                                                  tatTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATcc                                                                                     >  1:323644/1‑150 (MQ=255)
                                                                   atTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCGGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCa                                                                                    <  2:627070/150‑1 (MQ=255)
                                                                     tGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCACACAGGCTGCTGCCGTAGCCGCTGCCGCTGCACTTTCTCGACATCCATg                                                                                  >  2:848719/1‑150 (MQ=255)
                                                                      gAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAACGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTCGCCGATGCCGCTGCACTGTCTCGACATCCATGc                                                                                 >  2:1244323/1‑150 (MQ=255)
                                                                              aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCTCTGTTCCGGCGTTGCTAAACGCCGCAAAACGTTTCAAACAGGCTGCTACCGTAGCCGCTGCCGCTGCAATTTCTCGACATCCATGCTCTGCAAc                                                                         >  1:389104/1‑150 (MQ=37)
                                                                              aagaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCCAc                                                                         >  1:86990/1‑150 (MQ=255)
                                                                               agaaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACg                                                                        >  2:1510823/1‑150 (MQ=255)
                                                                                  aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCt                                                                     <  1:1812945/150‑1 (MQ=255)
                                                                                   tttGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCtt                                                                    <  2:529314/150‑1 (MQ=255)
                                                                                      gTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGt                                                                 >  2:560014/1‑150 (MQ=255)
                                                                                      gTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGt                                                                 >  2:1683280/1‑150 (MQ=255)
                                                                                        tggCCGGATAAGGCGTTTACGCCGCATCTGGAATAAACAAAGCGCACTGTTCCGGCGTTGATAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCg                                                               <  1:1764920/149‑1 (MQ=255)
                                                                                        aGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAACCCGGCTGCTGCCGTAGCCGATGCCGCTGCCCTTTCTCGACATCCATGCACTGCAACGCCTTTGTCg                                                               >  1:1553620/1‑150 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCgg                                                              <  1:1790988/150‑1 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCgg                                                              >  1:781397/1‑150 (MQ=255)
                                                                                         ggCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACGTTATCGACATCCATGCCCTGCACCGCCTTTGTCgg                                                              >  2:1032025/1‑150 (MQ=255)
                                                                                           ccGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCCCTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGc                                                            >  1:1675853/1‑150 (MQ=255)
                                                                                             ggATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAg                                                          >  1:1950014/1‑150 (MQ=255)
                                                                                                tAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCt                                                       <  1:1590017/150‑1 (MQ=255)
                                                                                                tAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCt                                                       <  1:169621/150‑1 (MQ=255)
                                                                                                      gTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAaca                                                 >  1:529837/1‑150 (MQ=255)
                                                                                                       tttACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAacac                                                <  2:24971/150‑1 (MQ=255)
                                                                                                         tACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACCGGCTGCTGCCGTAGCCGATGCCGCTGCACTGTCTCGACCTCCCTGCCCTGCACCGCCTTTGTCGGGCCGGCTTCAACACAg                                              >  1:1616518/1‑150 (MQ=255)
                                                                                                          aCGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAgg                                             <  1:1145044/150‑1 (MQ=255)
                                                                                                           cgccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGc                                            >  1:12743/1‑150 (MQ=255)
                                                                                                            gccgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGcc                                           <  1:1351142/150‑1 (MQ=255)
                                                                                                              cgcATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCgg                                         <  1:560014/150‑1 (MQ=255)
                                                                                                                    tGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGt                                   >  2:1997698/1‑150 (MQ=255)
                                                                                                                        aTAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTcc                               <  2:1750475/150‑1 (MQ=255)
                                                                                                                         tAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTccc                              <  2:1486525/150‑1 (MQ=255)
                                                                                                                           aaCAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCCTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCGTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGa                            <  2:333315/150‑1 (MQ=255)
                                                                                                                           aaCAAAGCGCACTGTTCCGGCGTTGAGAAACGACGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGTCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGa                            <  1:428203/150‑1 (MQ=255)
                                                                                                                             cAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATg                          <  1:1186954/150‑1 (MQ=255)
                                                                                                                             cAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATg                          <  1:159042/150‑1 (MQ=255)
                                                                                                                             cAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGATTCAACACAGGCCTGTCCCGTCTCCCGATg                          <  1:324822/150‑1 (MQ=255)
                                                                                                                                aGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCa                       >  2:1369312/1‑150 (MQ=255)
                                                                                                                                 gcgcACTGTTCCGGCGTTGAGAACCGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAg                      <  2:829777/150‑1 (MQ=255)
                                                                                                                                  cgcACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTGTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGc                     <  2:1548733/150‑1 (MQ=255)
                                                                                                                                    cACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGcac                   <  2:80030/150‑1 (MQ=255)
                                                                                                                                    cACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGcac                   <  1:535642/150‑1 (MQ=255)
                                                                                                                                     aCTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGcaca                  >  1:627626/1‑150 (MQ=255)
                                                                                                                                        gTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGTCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGAt               >  2:1650771/1‑150 (MQ=255)
                                                                                                                                        gTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGAt               <  2:456668/150‑1 (MQ=255)
                                                                                                                                         ttCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATc              <  1:946363/150‑1 (MQ=255)
                                                                                                                                          tCCGGCGTTTCTCAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTAGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCg             >  1:1056598/1‑150 (MQ=255)
                                                                                                                                           ccGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGc            >  2:759027/1‑150 (MQ=255)
                                                                                                                                             ggCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCGTCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCAc          <  1:813459/150‑1 (MQ=255)
                                                                                                                                               cGTTGAGAAACGCCGGAAACCGTTTCAAACAGGCTGCTGCCGTCGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGTTCCCGTCTCCCGATGCCAGCCCAGCTCGCCCtt        >  2:1427418/1‑150 (MQ=255)
                                                                                                                                               cGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACGTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACtt        <  2:120889/150‑1 (MQ=255)
                                                                                                                                                gTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTa       >  1:1838516/1‑150 (MQ=255)
                                                                                                                                                 ttGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTaa      >  1:1488235/1‑150 (MQ=255)
                                                                                                                                                  tGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAt     <  1:773544/150‑1 (MQ=255)
                                                                                                                                                  tGAGAAACGCCGGAAAACGTTTCAAACAGGCGGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAAt     <  1:1506620/150‑1 (MQ=255)
                                                                                                                                                   gagaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTGGACGATGACTCTGCTCTTTCTCGCCATCCATGCACTGAAACGCCGTTGTATTGCAGGCGTAAAGCAAGGACTGTCCCGAGTAACTATGACAGAAAAGATCGCACtttctc    >  2:1459028/1‑146 (MQ=255)
                                                                                                                                                   gagaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATc    >  2:1488328/1‑150 (MQ=255)
                                                                                                                                                     gaAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGc  <  1:196635/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGCTGAAAGTCACTAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACATGATTTCTGCAATATATTGAATTTGGAAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCTGGCATAAACAAAGCGCACTGTTCCGGCGTTGAGAAACGCCGGAAAACGTTTCAAACAGGCTGCTGCCGTAGCCGATGCCGCTGCACTTTCTCGACATCCATGCACTGCAACGCCTTTGTCGGGCAGGCTTCAACACAGGCCGGTCCCGTCTCCCGATGCCAGCACAGATCGCACTTAATCGC  >  NC_000913/3740942‑3741240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: