Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,800 C→A 5.3% A580S (GCG→TCG)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,8000CA 5.3% 419.5 / 9.1 140A580S (GCG→TCG) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (68/64);  new base A (6/2);  total (74/66)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 141 total aligned reads displayed.

GTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACC  >  NC_000913/3832651‑3832947
                                                                                                                                                     |                                                                                                                                                   
gTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGc                                                                                                                                                     >  2:163055/1‑150 (MQ=255)
 tAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCa                                                                                                                                                    <  1:362429/150‑1 (MQ=255)
       tGCACATCGCCCGCCTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCa                                                                                                                                              <  1:154561/150‑1 (MQ=255)
        gCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAg                                                                                                                                             >  2:652007/1‑150 (MQ=255)
        gCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGAAGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAg                                                                                                                                             >  1:527516/1‑150 (MQ=255)
         cacaTCGCCCGCTTCCGGGAACCCCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGTCGGGCAAGGTAATCCCAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTGCGAACgcgcggg                                                                                                                                            <  1:1814407/150‑4 (MQ=255)
         cacaTCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTACCATGTATTGACGGTCAAGGTAATCACACGCCGGATCGTCCGGGAACCCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTGCGAACgcgcggg                                                                                                                                            <  1:800578/150‑4 (MQ=255)
          acaTCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCt                                                                                                                                           <  1:346904/150‑1 (MQ=255)
               gCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACg                                                                                                                                      >  2:204927/1‑150 (MQ=255)
               gCCCGCGTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCACGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACg                                                                                                                                      <  1:146388/150‑1 (MQ=255)
                cccGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCCTCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGa                                                                                                                                     >  1:1752051/1‑150 (MQ=255)
                 ccGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGAt                                                                                                                                    <  1:362983/150‑1 (MQ=255)
                 ccGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGAt                                                                                                                                    <  2:481683/150‑1 (MQ=255)
                 ccGCTTCAGTGAACACCGGCGCAACAATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGAt                                                                                                                                    >  1:1169898/1‑150 (MQ=255)
                      tCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGa                                                                                                                               >  1:1252584/1‑150 (MQ=255)
                         gTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATAc                                                                                                                            >  1:476120/1‑150 (MQ=255)
                          tGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACg                                                                                                                           <  1:633682/150‑1 (MQ=255)
                           gAACACCGGCGCAACCATCACGTAGTAGACTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGACCGGCAAATACATCATCATGGACAGCATAATCGGCGTACCCAGCGCGTTCGAACGCGCAGCTTCACGATACAGATACgg                                                                                                                          >  1:1337057/1‑150 (MQ=255)
                            aaCACCGGCTCAACCATCACGGTGTCGCCTAACATGTATTGACGGTCAAGGAAAACACACGCCGGAGCGGCCGGGCACTCCATCATCAAGGCGCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGc                                                                                                                         <  1:120913/150‑1 (MQ=255)
                              cacCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCCCGATACAGATACGGcat                                                                                                                       >  2:516402/1‑150 (MQ=255)
                              cacCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGcat                                                                                                                       >  2:2049523/1‑150 (MQ=255)
                                ccGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCATGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCAGGATACAGAGACGGcatcg                                                                                                                     >  2:462881/1‑149 (MQ=255)
                                ccGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGcatca                                                                                                                     <  1:409517/150‑1 (MQ=255)
                                      cAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGc                                                                                                               >  2:1599104/1‑150 (MQ=255)
                                        aCCATCTCGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCAt                                                                                                             >  2:842591/1‑150 (MQ=255)
                                         ccATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCCCGATACAGATACGGCATCATGCGGCAtt                                                                                                            >  2:1542066/1‑150 (MQ=255)
                                         ccATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCAtt                                                                                                            <  2:726108/150‑1 (MQ=255)
                                           aTCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCGCAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGAGTACCCCGCGCGTTCGCACGCGCAGCTTCACGAGACAGATAAGGCATCAAGCGGCATATc                                                                                                          >  1:1391479/1‑150 (MQ=255)
                                               cGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGtt                                                                                                      >  1:876711/1‑150 (MQ=255)
                                                gTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTg                                                                                                     <  1:1210769/150‑1 (MQ=255)
                                                  tGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCg                                                                                                   >  1:1528782/1‑150 (MQ=255)
                                                  tGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCg                                                                                                   <  2:297515/150‑1 (MQ=255)
                                                    tCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTg                                                                                                 <  1:597121/150‑1 (MQ=255)
                                                    tCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTg                                                                                                 <  1:1359691/150‑1 (MQ=255)
                                                    tCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTg                                                                                                 <  2:1200753/150‑1 (MQ=255)
                                                     cGCCTAACATGTATTGAAGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTga                                                                                                >  1:1959052/1‑150 (MQ=255)
                                                      gCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGCACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTAAGTTGCGTgaa                                                                                               >  1:65229/1‑150 (MQ=255)
                                                       ccTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTAACCCGCCCTTTCGCACGCGAAGCTACACGAAACAGATACTGAATCATGCGGCAGATCAGTTGCGTcaag                                                                                              >  1:1033500/1‑150 (MQ=255)
                                                        cTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTgaaga                                                                                             <  2:1403386/150‑1 (MQ=255)
                                                        cTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCAATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTgaaga                                                                                             >  2:62717/1‑150 (MQ=255)
                                                        cTAACATGTATTGACGGTCAAGGGAATCACAAGCCGGATCGTCCGGGAACTCCATCATCTTGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTgaaga                                                                                             <  2:1744577/150‑1 (MQ=255)
                                                          aaCATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGaagaag                                                                                           <  2:540508/150‑1 (MQ=255)
                                                          aaCATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGaagaag                                                                                           <  2:547997/150‑1 (MQ=255)
                                                              tGTATTGACTGTCAAGGTAATCACAAGCCGGATCGTCCGTGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCTCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGca                                                                                       <  2:1162223/150‑1 (MQ=255)
                                                               gTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcac                                                                                      <  2:1169897/150‑1 (MQ=255)
                                                               gTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcac                                                                                      >  1:1442303/1‑150 (MQ=255)
                                                                tATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcacc                                                                                     >  1:1930697/1‑150 (MQ=255)
                                                                tATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcacc                                                                                     >  1:645499/1‑150 (MQ=255)
                                                                 aTTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGcacca                                                                                    <  2:126898/150‑1 (MQ=255)
                                                                 aTTGAAGGTCAAGGTAATCACAATCAGGATCGTCCGGTAACTCCATCATCATTGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCAAGATTCATAAAAGGCATCAATCGGCATTTCAGTTGCGTGAAGAACCGcacca                                                                                    >  2:1247215/1‑150 (MQ=255)
                                                                  ttGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCTGCTTCACGATACAGATACGGCTTCATGCGGCATTTCAGTTGCGTGAAGAAGCGcaccac                                                                                   <  2:1080604/150‑1 (MQ=255)
                                                                   tGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACcaca                                                                                  <  1:747267/150‑1 (MQ=255)
                                                                    gACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACAt                                                                                 <  2:849589/150‑1 (MQ=255)
                                                                       ggTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcac                                                                              >  1:2040118/1‑150 (MQ=255)
                                                                       ggTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcac                                                                              <  1:939503/150‑1 (MQ=255)
                                                                        gTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATcaca                                                                             >  2:716551/1‑150 (MQ=255)
                                                                            aGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCCGTTGCGTGAAGAAGCGCACCACATCACAGGAc                                                                         >  1:1844793/1‑150 (MQ=255)
                                                                               tAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtca                                                                      >  2:914292/1‑150 (MQ=255)
                                                                                 aTCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCTTCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACtcatc                                                                    <  2:363204/150‑1 (MQ=255)
                                                                                   cacaAGCCGGATCGTCCGGGAACTCCATCATCAGGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTcatcat                                                                  >  2:2001860/1‑150 (MQ=255)
                                                                                    acaAGCCGGATCGTCCGGGAACTCCATCATCATGTCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCCCGATACAGATACGGCATCATGCGGCATTTCAGTTGCGAGAAGAACCGCCCCACATCACAGGACTCatcatc                                                                 >  1:109046/1‑150 (MQ=255)
                                                                                    acaAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCatcatc                                                                 <  1:127916/150‑1 (MQ=255)
                                                                                    acaAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCatcatc                                                                 >  1:492626/1‑150 (MQ=255)
                                                                                    acaAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCatcatc                                                                 >  1:1090997/1‑150 (MQ=255)
                                                                                    acaAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCatcatc                                                                 <  2:188536/150‑1 (MQ=255)
                                                                                           ggATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGcc                                                          <  2:274063/150‑1 (MQ=255)
                                                                                               cGTCCGGGAACTCCTTCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACg                                                      <  1:1599105/150‑1 (MQ=255)
                                                                                               cGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACg                                                      >  1:1921176/1‑150 (MQ=255)
                                                                                                gTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACgg                                                     >  1:643707/1‑150 (MQ=255)
                                                                                                  ccGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATCCGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACCTCACAGGACTCCTCATCGTAGGCCCACGGca                                                   >  1:712086/1‑150 (MQ=255)
                                                                                                  ccGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGCAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGcc                                                   >  2:981934/1‑149 (MQ=255)
                                                                                                  ccGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGca                                                   >  2:624513/1‑150 (MQ=255)
                                                                                                      gAACTCCATCATCATGGCCCGCATCCTCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACg                                               <  2:836332/150‑1 (MQ=255)
                                                                                                         cTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATa                                            >  2:1925833/1‑150 (MQ=255)
                                                                                                         cTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATa                                            >  1:164604/1‑150 (MQ=255)
                                                                                                         cTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCAAGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATa                                            >  2:326677/1‑150 (MQ=255)
                                                                                                                atcatGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTg                                     <  1:462881/150‑1 (MQ=255)
                                                                                                                        ccGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCgtg                             <  1:716552/150‑1 (MQ=255)
                                                                                                                            atcatcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATCCGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTaaa                         <  1:72460/150‑1 (MQ=255)
                                                                                                                             tcatcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAAc                        <  1:1746930/150‑1 (MQ=255)
                                                                                                                              catcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGCAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACg                       >  2:1521992/1‑150 (MQ=255)
                                                                                                                               ctcGGCGTACCACGCGCGTTCGCACGCGCAGCTGCTCTTTACTGATACGGCTTCATGCGGCATTTCAGGTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACgg                      <  2:1337056/149‑1 (MQ=255)
                                                                                                                                tcGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGc                     <  2:426818/150‑1 (MQ=255)
                                                                                                                                 cGGCGTACCCCGCGCGTTCGCACGCGCCGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCt                    >  2:1128715/1‑150 (MQ=255)
                                                                                                                                 cGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACTGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCt                    <  2:708171/150‑1 (MQ=255)
                                                                                                                                       aagctgcgcgTGCGAACGCGCGGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCCCAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCt              >  2:911371/6‑150 (MQ=255)
                                                                                                                                        agctgcgcgTGCGAACGCGCAGCTTCACGATACAGATACGGCATCATACGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTg             >  2:319192/5‑150 (MQ=255)
                                                                                                                                         gctgcgcgTGCGAACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGAGTAAACGGCTATGGCTgg            >  2:564165/4‑150 (MQ=255)
                                                                                                                                         cccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTgg            <  2:783403/150‑1 (MQ=255)
                                                                                                                                         cccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTgg            >  1:1168953/1‑150 (MQ=255)
                                                                                                                                         cccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTgg            >  1:1453089/1‑150 (MQ=255)
                                                                                                                                          ccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGga           >  2:981976/1‑150 (MQ=255)
                                                                                                                                          ccGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGga           <  2:18021/150‑1 (MQ=255)
                                                                                                                                           cgcgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGgag          <  1:993211/150‑1 (MQ=255)
                                                                                                                                             cgcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGATGAAGCGCACCACATCACAGGTCTCATCATCGTAGGCCGACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGagag        <  1:303497/150‑1 (MQ=255)
                                                                                                                                              gcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTgg            <  1:310284/145‑1 (MQ=255)
                                                                                                                                              gcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGc       <  1:204927/150‑1 (MQ=255)
                                                                                                                                              gcgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGc       >  2:1818616/1‑150 (MQ=255)
                                                                                                                                               cgTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGca      >  1:1607971/1‑150 (MQ=255)
                                                                                                                                                   cGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGCAGAGcaaacc  >  1:248505/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTAGAACTGCACATCGCCCGCTTCAGTGAACACCGGCGCAACCATCACGTTGTCGCCTAACATGTATTGACGGTCAAGGTAATCACAAGCCGGATCGTCCGGGAACTCCATCATCATGGCCCGCATCATCGGCGTACCCCGCGCGTTCGCACGCGCAGCTTCACGATACAGATACGGCATCATGCGGCATTTCAGTTGCGTGAAGAAGCGCACCACATCACAGGACTCATCATCGTAGGCCCACGGCACACGATAAGATTTGCTACCGTGTAAACGGCTATGGCTGGAGAGCAAACC  >  NC_000913/3832651‑3832947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑