Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,891,506 C→G 5.3% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,891,5060CG 5.3% 399.8 / 9.9 127intergenic (+23/‑109)tnaB/mdtLtryptophan transporter of low affinity/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (52/66);  new base G (4/3);  total (58/69)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 131 total aligned reads displayed.

GTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACT  >  NC_000913/3891357‑3891655
                                                                                                                                                     |                                                                                                                                                     
gTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTc                                                                                                                                                       >  2:914697/1‑150 (MQ=255)
  ttCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGc                                                                                                                                                     >  1:1598274/1‑150 (MQ=255)
    cccATTCAGATGTTCACGGTATGGGGCGGCATTCTTATTCCGGGGATTGTCATTCTCTTTGGTATAACCTTGATTTTGGGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTg                                                                                                                                                   <  1:234974/150‑1 (MQ=255)
    cccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCTTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTg                                                                                                                                                   <  1:470616/150‑1 (MQ=255)
    cccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTg                                                                                                                                                   >  2:631397/1‑150 (MQ=255)
    cccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTACGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTg                                                                                                                                                   >  2:1015218/1‑150 (MQ=255)
     ccAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTgg                                                                                                                                                  >  2:751632/1‑150 (MQ=255)
      cAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                                 >  1:1850733/1‑150 (MQ=255)
      cAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGc                                                                                                                                                 <  1:1072067/150‑1 (MQ=255)
         tCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGcttc                                                                                                                                              >  2:1736959/1‑150 (MQ=255)
           cgATGTACACCGTCGGGGGCGGCACTCTTCTGCCGTCGATTGGAATTCTATTTGGTATAACCGTGATTTTGTGCTGGTCCGGCAACGGCTTTCAGGTGTTACCTAAATTTTGCTAACTCCTTAAAGAAGCCAGCGAAGGGCTGGcttctt                                                                                                                                            <  1:813918/149‑1 (MQ=255)
            gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                           >  2:49650/1‑150 (MQ=255)
            gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                           >  1:1743375/1‑150 (MQ=255)
            gATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                           <  1:149514/150‑1 (MQ=255)
            gATGTTCACGGTCGGGTGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                           <  2:24299/150‑1 (MQ=255)
            gATGTTCACGGTCGGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTg                                                                                                                                           <  1:568232/150‑1 (MQ=255)
              tGTTCACGGACTGGGGCGGCAAGCTTATTCCGGAGATAGTCATGCTCTTTGGTATAACCGTGATGTTGTGCTGGTTCGGCAACGGCTTTAACGGGTTACCTACCTTTGGCTTAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGcc                                                                                                                                         <  1:1013696/150‑1 (MQ=255)
                ttCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGcgtg                                                                                                                                       >  1:422584/1‑147 (MQ=255)
                   actgTCTGGGGAGGCTATCTTATTCCGGCGTTTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGGTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCa                                                                                                                                    <  1:1138262/147‑1 (MQ=255)
                   aCGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCa                                                                                                                                    <  1:209055/150‑1 (MQ=255)
                   aCGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAg                                                                                                                                   <  1:922661/150‑1 (MQ=255)
                     ggTCTGGGTCGGCAATCTTTTTCCGGGGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGGTGGTTCGGCAACGTCTTTAACGTTTTACCTATATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAgg                                                                                                                                  <  2:2000467/150‑1 (MQ=255)
                         tGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAAt                                                                                                                              >  1:1771227/1‑150 (MQ=255)
                          ggggCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATc                                                                                                                             >  1:914798/1‑150 (MQ=255)
                          ggggCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATc                                                                                                                             >  2:1924729/1‑150 (MQ=255)
                          ggggCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATc                                                                                                                             >  2:1988758/1‑150 (MQ=255)
                              gggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACtt                                                                                                                         <  2:779289/149‑1 (MQ=255)
                               ggcAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTa                                                                                                                        <  2:1924004/150‑1 (MQ=255)
                                gcAATCTTATTCCGGCTATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTAt                                                                                                                       <  2:2004905/150‑1 (MQ=255)
                                 cAATCTTATTCCGGCGATTGTCATTCTCTTTTGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATg                                                                                                                      <  1:1043847/150‑1 (MQ=255)
                                 cAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATg                                                                                                                      <  1:1179241/150‑1 (MQ=255)
                                  aaTCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGt                                                                                                                     >  1:516965/1‑150 (MQ=255)
                                   aTCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTc                                                                                                                    <  2:1201073/150‑1 (MQ=255)
                                     cTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCa                                                                                                                  <  1:1170488/150‑1 (MQ=255)
                                         ttCCGGCGATTGTCATTCTCTTTGGTATAACCGTGAGTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGCCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATg                                                                                                              <  1:399959/150‑1 (MQ=255)
                                          tCCGGCGATTGTCATTCTCTTTGGGATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATgg                                                                                                             >  2:602543/1‑150 (MQ=255)
                                            cgtcGATTTTCATTCTCTTTTGTATAACCGTTATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAAATTGCCTAAATCCATAAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCa                                                                                                           <  1:319972/147‑1 (MQ=255)
                                                gATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTc                                                                                                       <  2:706274/150‑1 (MQ=255)
                                                   tggcATTCTCTTTGGTATAACGGTGATTTTGTGCTGGGTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTGCAAGAAGCCAGCCAATCGCTGGCTTCTTGCCTCTCAGGATATCACTTATGTCCAAATGGCAACTCGcc                                                                                                    <  1:146432/147‑1 (MQ=255)
                                                   tGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGcc                                                                                                    <  1:477998/150‑1 (MQ=255)
                                                       aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                                >  1:1611448/1‑150 (MQ=255)
                                                       aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACATCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                                <  2:1555600/150‑1 (MQ=255)
                                                       aTTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACATCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAt                                                                                                >  1:205158/1‑150 (MQ=255)
                                                        ttCTCTTTGGTATAACCGTGATTTTGTGCTGGATCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtc                                                                                               <  2:2020413/150‑1 (MQ=255)
                                                         tctcTTTGGTCTAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTAACTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcc                                                                                              <  2:372331/150‑1 (MQ=255)
                                                          ctctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcct                                                                                             <  1:489177/150‑1 (MQ=255)
                                                          ctctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcct                                                                                             >  2:807804/1‑150 (MQ=255)
                                                            ctTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTAGACTGAtcctcc                                                                                           >  1:240449/1‑150 (MQ=255)
                                                            atttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGAtcctcc                                                                                           <  2:899772/149‑1 (MQ=255)
                                                             tttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATcctcct                                                                                          <  2:38350/150‑1 (MQ=255)
                                                              ttGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCtt                                                                                         <  2:1666130/150‑1 (MQ=255)
                                                                   ataACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTcacca                                                                                    >  2:2001085/1‑150 (MQ=255)
                                                                      aCCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGt                                                                                 >  1:1483403/1‑150 (MQ=255)
                                                                      aCCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGt                                                                                 >  2:881183/1‑150 (MQ=255)
                                                                       ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTa                                                                                >  1:374469/1‑150 (MQ=255)
                                                                       ccGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTa                                                                                >  2:25125/1‑150 (MQ=255)
                                                                           gATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTATATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCATCTCAGGAAATCACTTAAGTCCAAATGGCAACTCGCCTGATCCTCCTTCACAACGTATGCt                                                                            >  1:323606/1‑150 (MQ=255)
                                                                             ttttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTAGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCATCCTTCACCACGTAATCttt                                                                          >  1:348449/1‑150 (MQ=255)
                                                                              tttGTGCTGGTTCGGCAACGTCTTTAACGTGTTACATAAATTTGGCTAAATACTTCAAGAAGCAAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTg                                                                         <  2:1246312/150‑1 (MQ=255)
                                                                                tgtgCTGGTCCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCg                                                                       <  1:1015219/150‑1 (MQ=255)
                                                                                  tgCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTc                                                                     <  1:698380/150‑1 (MQ=255)
                                                                                    cTGGTTCGGCAACGTCGTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGATTTGCGTCAc                                                                   >  2:674513/1‑150 (MQ=255)
                                                                                        ttCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTa                                                               >  1:1962390/1‑150 (MQ=255)
                                                                                            gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCCCGTATGCTTTGCGTCACCTTACTAt                                                           >  1:763657/1‑150 (MQ=255)
                                                                                            gCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTAt                                                           >  1:1295406/1‑150 (MQ=255)
                                                                                              aaCGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCa                                                         <  2:551652/150‑1 (MQ=255)
                                                                                                   cTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACg                                                    >  2:1461985/1‑150 (MQ=255)
                                                                                                    tttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGc                                                   <  2:1398478/150‑1 (MQ=255)
                                                                                                     ttAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCt                                                  <  1:739461/150‑1 (MQ=255)
                                                                                                      tAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGCCGCtt                                                 >  2:140674/1‑150 (MQ=255)
                                                                                                      tAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTACTTGCCTCTCAGGAAAACACTAATGTCCAAATGGCAAATCTCCTGATCCTCGTTAACCACGTATGATTTGCGTCACATTACTATCAGGACGCtt                                                 >  2:1028057/1‑150 (MQ=255)
                                                                                                         cGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAg                                              <  2:1347619/150‑1 (MQ=255)
                                                                                                         cGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAg                                              <  1:49650/150‑1 (MQ=255)
                                                                                                         cGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTACTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCATTAg                                              >  2:305856/1‑150 (MQ=255)
                                                                                                           tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGcc                                            <  2:853764/150‑1 (MQ=255)
                                                                                                           tgtTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTGGCGTCACCTTACTATCAGGACGCTTTAGcc                                            <  1:467039/150‑1 (MQ=255)
                                                                                                             ttACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCa                                          <  1:1255346/150‑1 (MQ=255)
                                                                                                              tACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCAt                                         <  1:1797472/150‑1 (MQ=255)
                                                                                                                ccTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGt                                       <  1:609957/150‑1 (MQ=255)
                                                                                                                ccTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGGCCAAATGGCAACTCGCCTGATACTCCGTCACCACGTATGCTTTGCGTCACCGTACTATCATGACGCTTTAGCCAATGt                                       >  1:1040557/1‑150 (MQ=255)
                                                                                                                         ggCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCtttt                              >  1:1088429/1‑150 (MQ=255)
                                                                                                                          gCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCttttt                             <  2:570145/150‑1 (MQ=255)
                                                                                                                           cTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACAACGAATGCTTTGCGTCACCTTACTATCAGGACGCTTGAGCCCCTGTCCCGCGTTTTg                            >  2:117336/1‑150 (MQ=255)
                                                                                                                             aaaTCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGAt                          >  2:1310282/1‑150 (MQ=255)
                                                                                                                              aaTCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGAtt                         <  1:1507744/150‑1 (MQ=255)
                                                                                                                               aTCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGAttt                        <  2:750372/150‑1 (MQ=255)
                                                                                                                                    gcAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGt                   >  2:621281/2‑150 (MQ=255)
                                                                                                                                    gcAAGAAGCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCACCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCAGGTCCCGCTTTTTGATTTTTAGt                   >  1:1280935/2‑150 (MQ=255)
                                                                                                                                      aagaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGttt                 >  2:930219/1‑150 (MQ=255)
                                                                                                                                      aagaagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCGCGTATGCTTTGCGTCCCCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTGTTTGATTTGTAGtgt                 >  2:1887917/1‑148 (MQ=255)
                                                                                                                                        gaagCCAGCGAATGGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTg               >  2:1122917/1‑150 (MQ=255)
                                                                                                                                        gaagCCACCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCCCTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTg               <  2:378558/150‑1 (MQ=255)
                                                                                                                                         aagCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGc              <  1:1073181/150‑1 (MQ=255)
                                                                                                                                           gccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGccc            <  2:1743374/150‑1 (MQ=255)
                                                                                                                                           gccagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGccc            >  2:1625782/1‑150 (MQ=255)
                                                                                                                                             cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCCCCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTg          >  2:310776/1‑150 (MQ=255)
                                                                                                                                             cagccaTTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTg          >  1:637053/1‑150 (MQ=255)
                                                                                                                                                   ttCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTa    <  1:1918982/150‑1 (MQ=255)
                                                                                                                                                    tCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTAc   <  2:1296750/150‑1 (MQ=255)
                                                                                                                                                     cGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACt  <  1:1653855/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATTTTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAAATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTGATCCTCCTTCACCACGTATGCTTTGCGTCACCTTACTATCAGGACGCTTTAGCCCATGTCCCGCTTTTTGATTTGTAGTTTTGCCCTGGTTTTACT  >  NC_000913/3891357‑3891655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: