Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,896,350 G→T 5.3% A87E (GCA→GAA)  adeP ← adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,896,3500GT 5.3% 373.3 / 8.1 123A87E (GCA→GAA) adePadenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (65/51);  new base T (5/2);  total (70/53)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 126 total aligned reads displayed.

TCATCCAGTAGCGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG  >  NC_000913/3896201‑3896499
                                                                                                                                                     |                                                                                                                                                     
tcatcCAGTAGCGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTg                                                                                                                                                       <  2:1030665/150‑1 (MQ=255)
 catcCAGTAGCGAACGCGGAAACACGTCAGAAACAGCAGACCTATCGCGCCCCAGAAGAGTGAGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGAACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGTGAGCCAGTGc                                                                                                                                                      <  1:116030/150‑1 (MQ=255)
  atcCAGTAGCGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCa                                                                                                                                                     <  1:41752/150‑1 (MQ=255)
  atcCAGAAGCTAACGCGGAAAATCGTCTGTACCAGCAGAGCTCTCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAAAGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCa                                                                                                                                                     <  1:1698103/150‑1 (MQ=255)
         aGCGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGCCCTGCCACGGCCAGCCCATCGCCGGTGCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCa                                                                                                                                              >  2:32859/1‑150 (MQ=255)
         aGCGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCa                                                                                                                                              >  1:494619/1‑150 (MQ=255)
           cGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAgg                                                                                                                                            <  1:914227/150‑1 (MQ=255)
            gAACGCGGAAAATCGTGAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGt                                                                                                                                           <  1:1694285/150‑1 (MQ=255)
            gAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTTGCACTGGCaccc                                                                                                                                           <  1:642223/150‑4 (MQ=255)
             aaCGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCGCAGAAGATTGCGCCCATCCCGCCCTGCCACGGCAAGCCCATCGCCTGTGCAACGACAAAAGCGAAGAACGCATTCCGGCCCATAGCGGGTGCCAGTGCTACGGGCAGGtt                                                                                                                                          >  2:87342/1‑150 (MQ=255)
              aCGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCGTAGCGGGTGCCCGTGCCTCTGGCAGGTTa                                                                                                                                         >  1:1298730/1‑150 (MQ=255)
                 cgGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGCCCTGCCACGGCAAGCCCAGCGCCTGTACAACGACAAAAGCGGAGCCCGCCGTCAGGCCCATCGCGGGTGCCAGGGCAACTGGCAGGGTaccc                                                                                                                                      >  2:330312/1‑147 (MQ=255)
                  ggAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGGGCAACGGGCAGGTTAGCag                                                                                                                                     >  2:1595674/1‑149 (MQ=255)
                  ggAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACACATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCaa                                                                                                                                     >  1:1613534/1‑150 (MQ=255)
                  agAAAATCGTCAGAAACAGCAGACCTATATCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCAGTACAAAGCCAATAGCGAAGACCGCATTCAGGACCATAGCGGGTGCCAGTGAAACTCGCAGGTTAGCaa                                                                                                                                     >  1:1250748/2‑150 (MQ=255)
                     aaaTCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACa                                                                                                                                  <  1:640621/150‑1 (MQ=255)
                      aaTCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAg                                                                                                                                 <  1:1823971/150‑1 (MQ=255)
                         cGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTcc                                                                                                                              <  2:1708789/150‑1 (MQ=255)
                          gTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTccc                                                                                                                             >  1:716672/1‑150 (MQ=255)
                          gTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATACAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTcgc                                                                                                                             >  1:1597619/1‑148 (MQ=255)
                            cactAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCACGACCTGGCACGGCAAGCCCATCGCCTGTATAACGACTAAAGCGTAGAACTCATTCAGGCCCATAGCTGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcat                                                                                                                           <  1:350412/147‑1 (MQ=255)
                            cAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCAGACCTGCCACCGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGACACTGGCAGGTTAGCAAACAGTCCcaa                                                                                                                           >  1:1013708/1‑149 (MQ=255)
                              gTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcatca                                                                                                                         <  1:1151584/150‑1 (MQ=255)
                              gTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGGCCcatca                                                                                                                         >  2:213049/1‑150 (MQ=255)
                               tAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCcatcat                                                                                                                        <  2:823621/150‑1 (MQ=255)
                                     cagACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACt                                                                                                                  <  1:1315127/150‑1 (MQ=255)
                                     cagACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACt                                                                                                                  <  1:262284/150‑1 (MQ=255)
                                            aTCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATACCGGGTGCCAGAGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATAATGCCGAAt                                                                                                           >  2:429194/1‑150 (MQ=255)
                                             tCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATg                                                                                                          >  1:1709339/1‑150 (MQ=255)
                                                cgcCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAg                                                                                                       <  2:266196/150‑1 (MQ=255)
                                                 gcCCCAGAAGATGGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATCGCGGGTGACAGGGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATCCTGCCGAATGCAGc                                                                                                      <  2:216514/150‑1 (MQ=255)
                                                  ccccAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCg                                                                                                     >  2:143851/1‑150 (MQ=255)
                                                    ccAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGAt                                                                                                   >  2:430460/1‑150 (MQ=255)
                                                     cAGAAGATTGCGCCCATCCAGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATc                                                                                                  <  1:295885/150‑1 (MQ=255)
                                                       gaagaTTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGTAGCGATCAg                                                                                                <  2:1556322/150‑1 (MQ=255)
                                                         agaTTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAc                                                                                              >  2:72501/1‑150 (MQ=255)
                                                           aTTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGGCAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCCGACAg                                                                                            >  2:1437799/1‑150 (MQ=255)
                                                           aTTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAg                                                                                            <  2:1450840/150‑1 (MQ=255)
                                                            ttGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAgg                                                                                           <  2:1723473/150‑1 (MQ=255)
                                                              gcgcCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                                                                         >  1:1901063/1‑150 (MQ=255)
                                                              gcgcCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTa                                                                                         >  2:1588964/1‑150 (MQ=255)
                                                               cgcCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAg                                                                                        >  2:1570104/1‑150 (MQ=255)
                                                                 cccATCCCGACCTGCCACGGCAAGCCGATCGCCTGTACAACGACAAAAGCGAAGCACGCATTCAGGACCATAGCGGGTGCCAGAGCAACTGGCAGGTTAGCAGACAGTCCCATCATAATACTGCCGCATGAAGCGATCAGCCAGGGAGTg                                                                                      >  1:1444420/1‑150 (MQ=255)
                                                                 cccATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTg                                                                                      >  1:1972524/1‑150 (MQ=255)
                                                                   cATCCCGACCTGCCACGGCAAGCCCCTCGCCTGTACAACCCCAACCCCCACGCTCGCATTCAGGCCCATAGCGCGGGCCAGTGCAACAGGCAGGTAAGCAAACAGTCCCATCATAAACATGCCGAATGCAGCGATAAGACATGCAGTGAc                                                                                    <  2:1286291/150‑1 (MQ=255)
                                                                    aTCCCGACCTGCGACGGCAAGCCCATCGCCTGTACAACGACACAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACg                                                                                   <  2:160236/150‑1 (MQ=255)
                                                                    aTCCCGACCTGCCACGGCAAGCCAATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACAGGCAGGTTAGCAAACAGTCCCATCATAAAACTGCCCAATGCAGCGATCAGACAGGTAGTGACg                                                                                   >  1:13892/1‑150 (MQ=255)
                                                                       ccGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAg                                                                                <  1:2010181/150‑1 (MQ=255)
                                                                         gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAc                                                                              >  1:1068125/1‑150 (MQ=255)
                                                                         gACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCAGCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGAc                                                                              >  2:1046808/1‑150 (MQ=255)
                                                                            cTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGgc                                                                           >  2:1508939/1‑150 (MQ=255)
                                                                              gCCACGGCAAGCCCCTCGCCTGTACAACGACAAAAGCGAAGAACGCAGTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAACCAGTCCCATCATAATACGGCCGAATGCAGCGATCAGACAGGTAGAGACGAAGACGgcgc                                                                         >  1:477336/1‑150 (MQ=255)
                                                                              gCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGgcgc                                                                         >  1:824611/1‑150 (MQ=255)
                                                                                cACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTg                                                                       <  1:501441/150‑1 (MQ=255)
                                                                                 aCGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGCCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAATCAGTCCCATCATAATACTGCCGAATGCAGCGCGCAGACAGGTAGTGACGAAGACGGCGCTgg                                                                      <  1:123763/150‑1 (MQ=255)
                                                                                   ggCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTa                                                                    <  2:606250/150‑1 (MQ=255)
                                                                                   ggCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTa                                                                    <  2:1210352/150‑1 (MQ=255)
                                                                                    gCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTAt                                                                   >  1:1094033/1‑150 (MQ=255)
                                                                                        gCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCAt                                                               >  2:1930902/1‑150 (MQ=255)
                                                                                           cATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGcc                                                            <  2:1885369/150‑1 (MQ=255)
                                                                                              cGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGc                                                         <  2:1633793/150‑1 (MQ=255)
                                                                                                 cTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGCATGGATACCAGcgcc                                                      <  1:1996938/150‑4 (MQ=255)
                                                                                                  tGTACAACGACAAAAGCGATGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCAATCATAATAGTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGCGGGCGCTGGTATCCATGCCAGCAACg                                                     >  2:225453/1‑150 (MQ=255)
                                                                                                     aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCa                                                  <  1:213049/150‑1 (MQ=255)
                                                                                                     aCAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGGTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAATCCGGCGCGGGTATCCATGCCAGCAACGCCa                                                  >  1:185866/1‑150 (MQ=255)
                                                                                                      cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCaa                                                 >  2:1356194/1‑150 (MQ=255)
                                                                                                      cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCaa                                                 >  1:468144/1‑150 (MQ=255)
                                                                                                      cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCaa                                                 >  2:1090421/1‑150 (MQ=255)
                                                                                                      cAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCAATGCCAGCAACGCCaa                                                 >  1:1022630/1‑150 (MQ=255)
                                                                                                         cGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGCCAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCACCGCCAAGaa                                              >  1:77453/1‑150 (MQ=255)
                                                                                                          gACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATTCTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGAATCCATGCCAGCAACGCCAAGAAt                                             >  1:108911/1‑150 (MQ=255)
                                                                                                            aaaaaGCGAAGAACGCATTCAGGCCCATAGCGGGGGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAAttt                                           <  2:523431/149‑1 (MQ=255)
                                                                                                               aaGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCg                                        >  2:1346899/1‑150 (MQ=255)
                                                                                                                   gaagaaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGtt                                    >  1:1662248/1‑150 (MQ=255)
                                                                                                                       aaCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACa                                <  1:702150/150‑1 (MQ=255)
                                                                                                                        aCGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaa                               >  1:585218/1‑150 (MQ=255)
                                                                                                                         cGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGAGGGTTAACaaa                              >  1:1150718/1‑150 (MQ=255)
                                                                                                                         agaaTTCAGCCCCATAGCGGGAGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATACTACTGCCGAATGCAGCGATCACACAGGTAGTGACGAAGACGGCGCTTGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACaaa                              <  2:331336/147‑1 (MQ=255)
                                                                                                                           cATTCAGACCCATAGTGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATTCCAGCAACGCCAAGAATTTGCGGGTTAACaaaaa                            >  2:1610590/1‑150 (MQ=255)
                                                                                                                            aTTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                           >  2:532361/1‑150 (MQ=255)
                                                                                                                            aTTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAAc                           <  2:730761/150‑1 (MQ=255)
                                                                                                                                  gCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTa                     >  1:718426/1‑150 (MQ=255)
                                                                                                                                  gCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACACCAAGAATTTGCGGGTTAACAAAAACGATGTa                     <  2:716671/150‑1 (MQ=255)
                                                                                                                                    ccATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGCCGGCGCTGGTATCCCTGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTaaa                   >  2:1092672/1‑150 (MQ=255)
                                                                                                                                          aacctGCCAGTTGCACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCg             >  2:503232/5‑150 (MQ=255)
                                                                                                                                          aacctGCCAGTTGCACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCg             >  2:775644/5‑150 (MQ=255)
                                                                                                                                           gggTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGt            >  2:431271/1‑150 (MQ=255)
                                                                                                                                            ggTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTc           <  2:1791717/150‑1 (MQ=255)
                                                                                                                                            cctGCCAGTTGCACTGGCAGGTTAGTAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTc           >  2:526163/3‑150 (MQ=255)
                                                                                                                                             gTGCCAGTTCAATTGGCATGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGCCAGGTTGTGACGAAGACGGATCTGGTATCCATGACAGCAACGCCAAGAATTTGCGGGTTAACTAAAAATATGTAAACCATCGTCa          <  2:1250747/150‑1 (MQ=255)
                                                                                                                                             gTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGAAAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCa          >  1:1465179/1‑150 (MQ=255)
                                                                                                                                             ctGCCAGTTGCACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCa          >  2:438544/2‑150 (MQ=255)
                                                                                                                                              tGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAg         <  2:1901062/150‑1 (MQ=255)
                                                                                                                                               gCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAgg        >  1:1391847/1‑150 (MQ=255)
                                                                                                                                                ccAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCAAAATACTGCCGAATGCAGCGATCTGACAGGTAGTGACGAAGACGGCGCTGGTATCGATGCCAGCAACGCAAAGAATTTGCGGGTTAACAAAAACGATGTAAACCAGCGTCAGGa       >  2:323618/1‑150 (MQ=255)
                                                                                                                                                 cAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGaa      >  1:220622/1‑150 (MQ=255)
                                                                                                                                                 cAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGaa      >  1:435512/1‑150 (MQ=255)
                                                                                                                                                    tGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGTTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggt   <  1:872991/150‑1 (MQ=255)
                                                                                                                                                     gCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAggtg  <  1:1570105/150‑1 (MQ=255)
                                                                                                                                                     gCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTAAGGAAggtg  >  2:1258970/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCCAGTAGCGAACGCGGAAAATCGTCAGTAACAGCAGACCTATCGCGCCCCAGAAGATTGCGCCCATCCCGACCTGCCACGGCAAGCCCATCGCCTGTACAACGACAAAAGCGAAGAACGCATTCAGGCCCATAGCGGGTGCCAGTGCAACTGGCAGGTTAGCAAACAGTCCCATCATAATACTGCCGAATGCAGCGATCAGACAGGTAGTGACGAAGACGGCGCTGGTATCCATGCCAGCAACGCCAAGAATTTGCGGGTTAACAAAAACGATGTAAACCATCGTCAGGAAGGTG  >  NC_000913/3896201‑3896499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑