Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,937,143 A→T 7.7% D289V (GAT→GTT)  rbsB → D‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,1430AT 7.7% 379.4 / 17.0 130D289V (GAT→GTT) rbsBD‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base A (57/63);  new base T (2/8);  total (59/71)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 134 total aligned reads displayed.

TGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAA  >  NC_000913/3936994‑3937292
                                                                                                                                                     |                                                                                                                                                     
tGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGa                                                                                                                                                       >  2:391948/1‑150 (MQ=255)
tGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGa                                                                                                                                                       >  2:684045/1‑150 (MQ=255)
tGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGa                                                                                                                                                       >  2:554125/1‑150 (MQ=255)
  aCGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATc                                                                                                                                                     <  2:201164/150‑1 (MQ=255)
     gTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGa                                                                                                                                                  <  2:1754621/150‑1 (MQ=255)
      tACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGaa                                                                                                                                                 <  2:401172/150‑1 (MQ=255)
      tACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGAGCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGaa                                                                                                                                                 <  1:1767038/150‑1 (MQ=255)
         acCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCTCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGAAACt                                                                                                                                              <  2:1219046/150‑1 (MQ=255)
          ccGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGTCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGAAACTg                                                                                                                                             <  2:1044891/150‑1 (MQ=255)
          ccGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTg                                                                                                                                             >  2:534478/1‑150 (MQ=255)
            ggATGGCGAAAATTCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGATAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGgt                                                                                                                                           <  1:396904/150‑1 (MQ=255)
             gATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGATCAGGCTAAGTATCCGGTTGATCTGAAACTGgtt                                                                                                                                          >  1:1081264/1‑150 (MQ=255)
              aTGGGGTAAAAGGGGGGAATGATGGCAAAGTCTCAGCGTCTATGGCTCAGCTACCCGATCAGATTTGCGCGAACGGCGGCTAAACCGCAGAGAATGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGgttg                                                                                                                                         <  2:339275/150‑1 (MQ=255)
                ggCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGAAACTGgttgtt                                                                                                                                       <  2:1344274/150‑1 (MQ=255)
                ggCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGgttgtt                                                                                                                                       >  1:2012522/1‑150 (MQ=255)
                 gCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGAAACTGGTTGTTa                                                                                                                                      <  2:1563378/150‑1 (MQ=255)
                 gCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTa                                                                                                                                      <  1:559640/150‑1 (MQ=255)
                 gCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTa                                                                                                                                      >  1:762227/1‑150 (MQ=255)
                    aaacaGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCGCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGc                                                                                                                                   <  1:957757/150‑1 (MQ=255)
                    aaaaaGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGc                                                                                                                                   <  1:239627/150‑1 (MQ=255)
                    aaaaaGCGGTGAAAGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGCTTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGc                                                                                                                                   <  1:1501844/150‑1 (MQ=255)
                     aaaaGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCa                                                                                                                                  <  1:1932272/150‑1 (MQ=255)
                        aGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCAGGTTGATCTGAAACTGGTTGTTAAGAagta                                                                                                                               >  1:30983/1‑150 (MQ=255)
                          cGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGtt                                                                                                                            <  2:583392/150‑1 (MQ=255)
                           ggTGAATGATGGCAAACAAGCATCGACTCTCGCTCAGCTACCGGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGtt                                                                                                                            <  2:1529349/150‑1 (MQ=255)
                             tGAATGATGGCAAACTAGCAGCGCCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGtttt                                                                                                                          <  2:739555/150‑1 (MQ=255)
                             tGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGtttt                                                                                                                          <  1:885066/150‑1 (MQ=255)
                                atgattGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGTCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAAt                                                                                                                       <  1:1449535/150‑1 (MQ=255)
                                  gatgGCAAACTAGCAGCGACTATCGCTCAGCAACCCGATCAGATTGGCGCGAAATGCGTCGAAACCGCATATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCa                                                                                                                     <  2:82171/150‑1 (MQ=255)
                                   atgGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAg                                                                                                                    <  2:2045558/150‑1 (MQ=255)
                                   atgGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAg                                                                                                                    >  2:751279/1‑150 (MQ=255)
                                       cAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTg                                                                                                                >  1:1569729/1‑150 (MQ=255)
                                       cAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATAGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTg                                                                                                                >  1:203162/1‑150 (MQ=255)
                                         aaCTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTa                                                                                                              <  2:1909148/150‑1 (MQ=255)
                                           cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATg                                                                                                            <  1:1443864/150‑1 (MQ=255)
                                           cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATg                                                                                                            <  1:452461/150‑1 (MQ=255)
                                                    aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATgg                                                                                                   <  1:1607241/150‑1 (MQ=255)
                                                    aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATgg                                                                                                   >  2:136902/1‑150 (MQ=255)
                                                       aTCGCTCAGCTACCCGATCAGATTGGAGCGATAGGCGTCGAAACCGCAGATATAGTGCTGAACGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGa                                                                                                <  1:310830/150‑1 (MQ=255)
                                                        gcGCTCAGCTACCCGATCTGATTGGCGCGAAAGGCGTCGAAACCGCAGACAAAGGGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGGTGAGCTGAAACTGGTTGTTAAGCCGTAGTTTTATTCAGGTTGTATGACCTGATGGTGAc                                                                                               <  2:288152/149‑1 (MQ=255)
                                                        acGCTCAGCTACCAGAGCATATTTGCTCGAAAGGGGTGGATACCTCTGATAATGTGCTGAAAGCCGAGATAGTTCAGGCTAAGTATCCTGTTGATCTCAAACTGGTTTTTATGCAGTAGTTTTATTCAGGTTGTATGACCTGATGGTGAc                                                                                               <  2:1198783/149‑1 (MQ=255)
                                                          gCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGAGCTGAAAGGCGAGAAAGATCAGGCTAACTATCGGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTATTAATCAGGTTGTATGACCTGATGGTGACAt                                                                                             >  2:90284/1‑150 (MQ=255)
                                                               gCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATa                                                                                        >  1:1102979/1‑150 (MQ=255)
                                                                cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAc                                                                                       >  2:1313165/1‑150 (MQ=255)
                                                                cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATAc                                                                                       >  2:105393/1‑150 (MQ=255)
                                                                 tACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                      <  1:337319/150‑1 (MQ=255)
                                                                 tACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                      >  1:1764548/1‑150 (MQ=255)
                                                                 tACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                      >  1:1851718/1‑150 (MQ=255)
                                                                 tACCAGATCAGAATGGCGCGAAATGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAATCTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACg                                                                                      <  2:1441017/150‑1 (MQ=255)
                                                                     cGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                                  >  1:1994459/1‑150 (MQ=255)
                                                                      gATCAGATTGGAGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCCTc                                                                                 <  2:1809392/150‑1 (MQ=255)
                                                                       aTCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCg                                                                                <  1:709676/150‑1 (MQ=255)
                                                                       aTCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCGGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCg                                                                                <  2:1866483/150‑1 (MQ=255)
                                                                       aTCAGATTGGCGCGAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGa                                                                               <  2:1569811/150‑1 (MQ=255)
                                                                           gATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAg                                                                            <  2:2002572/150‑1 (MQ=255)
                                                                               ggCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        >  1:1018048/1‑150 (MQ=255)
                                                                               ggCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        >  2:801593/1‑150 (MQ=255)
                                                                                gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGaa                                                                       >  1:1264481/1‑150 (MQ=255)
                                                                                 cgcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGTTCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAAc                                                                      <  2:1662809/150‑1 (MQ=255)
                                                                                 cgcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGCCGAGAAAGTTCACGCTAAGTATCCGGATGATCTGAAACTGGATGAAAAGAACTAGTTTTAATCAGCTTCTATGACCTGATGCTGACATAAATACGTCATCGAAATATGAAc                                                                      >  1:324755/1‑150 (MQ=255)
                                                                                 cgcgAAAGGCGTCAAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGGAGTTTTAATCTGGTTGTATGACCTGATGGTGACATAAATACTTCATCGACAGATGAAc                                                                      >  2:1281069/1‑150 (MQ=255)
                                                                                       aGGCGTCGAAACCGCAGACAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTaa                                                                >  1:1022964/1‑150 (MQ=255)
                                                                                        ggCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAATTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGGTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAat                                                               <  1:578252/150‑1 (MQ=255)
                                                                                           gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAAtata                                                            >  1:103768/1‑150 (MQ=255)
                                                                                           gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAAtata                                                            <  1:413043/150‑1 (MQ=255)
                                                                                           gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAAtata                                                            >  2:1724772/1‑150 (MQ=255)
                                                                                              gAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAg                                                         >  1:335014/1‑150 (MQ=255)
                                                                                              gAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAg                                                         <  2:1075696/150‑1 (MQ=255)
                                                                                              gAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAg                                                         <  1:633208/150‑1 (MQ=255)
                                                                                                aaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGGAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGaa                                                       <  2:1827656/150‑1 (MQ=255)
                                                                                                aaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGGAATATAAAGaa                                                       >  2:507420/1‑150 (MQ=255)
                                                                                                  ccGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGaaaa                                                     >  1:373627/1‑150 (MQ=255)
                                                                                                  ccGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGaaaa                                                     >  1:1213421/1‑150 (MQ=255)
                                                                                                      aGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGGTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAg                                                 <  2:1349283/150‑1 (MQ=255)
                                                                                                       gATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAgg                                                >  1:529430/1‑150 (MQ=255)
                                                                                                       gATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACATGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAgg                                                >  1:289401/1‑150 (MQ=255)
                                                                                                               gCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                        >  1:1174616/1‑150 (MQ=255)
                                                                                                               gCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                        >  1:1153218/1‑150 (MQ=255)
                                                                                                               gCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                        >  1:1067561/1‑150 (MQ=255)
                                                                                                                 tGAAAGGAGAGAAAGTTCAGGCTAAGTATCCGGATGATCTTAAACTGGTTGTTAAGAAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCTACAGATGAACGTGTAATATAAAGAAAATCAGGGCACTCGCCa                                      >  2:730114/1‑150 (MQ=255)
                                                                                                                  gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAc                                     >  1:217692/1‑150 (MQ=255)
                                                                                                                     aGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGATGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCt                                  >  2:19252/1‑150 (MQ=255)
                                                                                                                      ggCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTa                                 <  1:944002/150‑1 (MQ=255)
                                                                                                                        cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAac                               >  1:1494701/1‑150 (MQ=255)
                                                                                                                            aaaGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACgg                           <  2:762226/150‑1 (MQ=255)
                                                                                                                               gTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGTAGGGCACGCGCCACCCTAACACGGTgg                        <  2:1213420/150‑1 (MQ=255)
                                                                                                                                  cAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcgc                     >  2:1831307/1‑150 (MQ=255)
                                                                                                                                  cAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGGCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcgc                     <  2:559317/150‑1 (MQ=255)
                                                                                                                                   aGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCGTGTTAGGGTGgcgcg                    <  1:602130/150‑2 (MQ=255)
                                                                                                                                   aGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGCTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCGTGTTAGGGTGgcgcg                    <  1:1408650/150‑2 (MQ=255)
                                                                                                                                    ggCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAt                   >  1:879410/1‑150 (MQ=255)
                                                                                                                                      cTAAGTATCCGGTTGTTCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGGTGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAttt                 >  2:1588728/1‑150 (MQ=255)
                                                                                                                                       tAAGTATCCGGTTGTTCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAtttt                >  1:1132001/1‑150 (MQ=255)
                                                                                                                                          gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATg             >  1:502088/1‑150 (MQ=255)
                                                                                                                                          gTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATg             >  1:8636/1‑150 (MQ=255)
                                                                                                                                          gTATCCGGGTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGATGTATGACCTGATGGTGACATAAAAACGTCATCGACAGATGAACGTGTAAAATAAAGAAAATCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATg             <  2:240451/150‑1 (MQ=255)
                                                                                                                                             tCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGGTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGAc          <  2:360249/150‑1 (MQ=255)
                                                                                                                                                ggTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATc       >  2:91424/1‑150 (MQ=255)
                                                                                                                                                    gATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACCCGGTGGCGCATTTTATGGACATCCCGa   >  2:1570401/1‑150 (MQ=255)
                                                                                                                                                     aTCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa  >  2:1125412/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGACGGTACACCGGATGGCGAAAAAGCGGTGAATGATGGCAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAA  >  NC_000913/3936994‑3937292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: