Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,947,049 A→T 5.2% intergenic (+17/+79) trpT → / ← hdfR tRNA‑Trp/flhDC operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,0490AT 5.2% 398.4 / 7.5 133intergenic (+17/+79)trpT/hdfRtRNA‑Trp/flhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (57/69);  new base T (4/3);  total (61/72)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 136 total aligned reads displayed.

TGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGC  >  NC_000913/3946901‑3947197
                                                                                                                                                    |                                                                                                                                                    
tgcctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGaa                                                                                                                                                     >  1:1055657/1‑150 (MQ=255)
tgcctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGaa                                                                                                                                                     >  1:1685599/1‑150 (MQ=255)
tgcctgTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGaa                                                                                                                                                     >  2:407233/1‑150 (MQ=255)
  cctgTCACGCAGGGGGTCGCGGGGTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAg                                                                                                                                                   <  2:84059/150‑1 (MQ=255)
        aCGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAgg                                                                                                                                             <  1:878946/150‑1 (MQ=255)
           cAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCACCCCTGCCAGAAATCATCCTTAGCTTTCGCTAAGGAtg                                                                                                                                          <  2:1664149/150‑2 (MQ=39)
               ggggCGCGGGCTCGAGTCCCGTCCGTACCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTTGGAGTTCGAGTCTCTCCTCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                      <  1:64140/150‑1 (MQ=255)
               gggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTCGAGCACCGGGCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                      <  1:925512/150‑1 (MQ=255)
               gggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                      <  2:1559855/150‑1 (MQ=255)
               gggTCGCGGGTTCGAGTCCCGGCCGTGCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTGCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                      <  2:1271335/150‑1 (MQ=255)
               gggTCGCGGGTGCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGGCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                                                                                      <  2:2041360/150‑1 (MQ=255)
                ggTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttttttt                                                                                                                                     <  2:2002019/150‑1 (MQ=255)
                  gcgcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                                                                                   <  1:1714622/149‑1 (MQ=255)
                    gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                                                                                 <  1:1907823/150‑1 (MQ=255)
                    gcgGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                                                                                 <  1:961047/150‑1 (MQ=255)
                       ggTTCGAGTCCCGTCCGAACCGCCACCCTAATTCGGGGCGTAGTTCATTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGCGAGTTCGAGGCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGa                                                                                                                              <  1:861781/150‑1 (MQ=255)
                           cGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                                                                          >  1:778966/1‑150 (MQ=255)
                             aGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                                                                                        >  2:2016652/1‑150 (MQ=255)
                                cccGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                                                                                     >  1:1563228/1‑150 (MQ=255)
                                cccGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                                                                                     >  1:621604/1‑150 (MQ=255)
                                cccGTCCGTTCCGCCACCCTAATTAGGGGAGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCAGCCCCTGCCAGATATCATCCTTAGCGAAAGCTAAGGATATTTTTTATCTGAAATGACCct                                                                                                                     >  2:387781/1‑150 (MQ=255)
                                 ccgtccgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                                                                                    >  1:557686/1‑150 (MQ=255)
                                      cgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGa                                                                                                               <  2:243029/150‑1 (MQ=255)
                                      cgtTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGa                                                                                                               <  1:1375964/150‑1 (MQ=255)
                                        ttCCGCCACCCTAATTAGGTGGGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCAGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                                                                             <  2:1197391/150‑1 (MQ=255)
                                        ttCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                                                                             <  2:1848844/150‑1 (MQ=255)
                                           cGCCACACTAATTAGTGGCGTAGTTCATTTGGTAGAGCACCGGGCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaa                                                                                                          <  2:622911/150‑1 (MQ=255)
                                             ccAGCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                                                                                        <  1:581448/150‑1 (MQ=255)
                                             ccACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                                                                                        <  1:479461/150‑1 (MQ=255)
                                                cccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                                                                                     <  2:495399/150‑1 (MQ=255)
                                                cccTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                                                                                     <  1:1004143/150‑1 (MQ=255)
                                                      ttAGGGGGGTAGTTTAATTGGGAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTTGCGAAATCTAAGGATTTTTTTTATCTGAATTAACCCTCTCCGAAGTAAATCCTTCTAcc                                                                                               <  1:1271590/150‑1 (MQ=255)
                                                      ttAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                                                                               >  1:1116287/1‑150 (MQ=255)
                                                        aGGTGCGTAGTTCAATTGGTAGAGCACCGGGCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg                                                                                             <  2:1462262/150‑1 (MQ=255)
                                                          gggCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGTATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                                                                           <  2:679643/150‑1 (MQ=255)
                                                          gggCGGAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGTGAGTTCGAGTCTCTCCGACCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTATTAATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                                                                           <  1:287758/150‑1 (MQ=255)
                                                           ggCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCGTTCTACCGGCAt                                                                                          >  2:109570/1‑150 (MQ=255)
                                                            gCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                                                                                         <  2:615492/150‑1 (MQ=255)
                                                             cGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                                                                                        <  2:529189/150‑1 (MQ=255)
                                                             cGTAGTTCAATTGGTAGAGCACCGGTATCCAAAACCGGGTGGTGGTAGTACGGGTCTCTCCGCCCCAGCAAGAAATCATCCTTAGCGAAAGCTAAGGTTTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                                                                                        >  1:1014564/1‑150 (MQ=255)
                                                                   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                                                                                  >  1:1143494/1‑150 (MQ=255)
                                                                     aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                                                                                <  1:662314/150‑1 (MQ=255)
                                                                          gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTc                                                                           <  1:634485/150‑1 (MQ=255)
                                                                             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata                                                                        <  2:1973524/150‑1 (MQ=255)
                                                                              agCACCGGTCTCCAAAACCGGGTGTTGTTAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat                                                                       <  1:1247444/150‑1 (MQ=255)
                                                                              agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat                                                                       <  1:760850/150‑1 (MQ=255)
                                                                               gCACCGGTCTCCAAAACCGTGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCTAAGTATATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt                                                                      <  1:1754065/150‑1 (MQ=255)
                                                                                 aCCGGTCTCCAAAACCGGGTTTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa                                                                    <  1:1882125/150‑1 (MQ=255)
                                                                                  ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt                                                                   >  1:905437/1‑150 (MQ=255)
                                                                                  ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt                                                                   >  2:1305007/1‑150 (MQ=255)
                                                                                    ggTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac                                                                 >  2:1934652/1‑150 (MQ=255)
                                                                                    ggTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac                                                                 <  1:379362/150‑1 (MQ=255)
                                                                                     gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTAGCCAGCCATTCATATTAATaca                                                                <  1:340880/150‑1 (MQ=255)
                                                                                        tcCAAAACCGGGTGTTGGGAGTTCGAGGCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt                                                             <  2:1743994/150‑1 (MQ=255)
                                                                                         ccAAAACCTGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc                                                            <  1:2043419/150‑1 (MQ=255)
                                                                                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc                                                            >  2:1028498/1‑150 (MQ=255)
                                                                                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc                                                            >  1:287436/1‑150 (MQ=255)
                                                                                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTACGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc                                                            >  1:214550/1‑150 (MQ=255)
                                                                                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCa                                                           >  2:34872/1‑150 (MQ=255)
                                                                                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAt                                                          >  2:883813/1‑150 (MQ=255)
                                                                                              aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCa                                                       <  1:539228/150‑1 (MQ=255)
                                                                                              aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCa                                                       <  2:1306768/150‑1 (MQ=255)
                                                                                                cGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGc                                                     <  2:1875130/150‑1 (MQ=255)
                                                                                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGtt                                                >  1:1122251/1‑150 (MQ=255)
                                                                                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGtt                                                <  1:522063/150‑1 (MQ=255)
                                                                                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATACTTCTACCGGCATCCTTGCAAGCCATTCATATTAATACACTTCATCAAGCACGtt                                                >  1:1951139/1‑150 (MQ=255)
                                                                                                       tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTTTCTGAAATAACCCTCTCCGAAGGACATCCGGCTACCCGCAGCCTTGCCAGCCATTCATATTAATACACTTACTACAGCACGTTaa                                              >  2:1237558/1‑150 (MQ=255)
                                                                                                             ttCGAGTCTCTCCGACCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCa                                        <  2:142759/150‑1 (MQ=255)
                                                                                                              tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCaa                                       >  1:833676/1‑150 (MQ=255)
                                                                                                              tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCaa                                       >  1:2016814/1‑150 (MQ=255)
                                                                                                                gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCATCTCCGAAGTAAATCCTTCTACCGGCCTCCTTGCCAGCCCTTCATATTAATACACTTCATACAGCACGTTAATTTTCaaaa                                     >  1:1569881/1‑150 (MQ=255)
                                                                                                                 aGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAg                                    <  2:778965/150‑1 (MQ=255)
                                                                                                                   tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGAt                                  <  2:1956194/150‑1 (MQ=255)
                                                                                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTACGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAATGATcg                                >  2:1247774/1‑150 (MQ=255)
                                                                                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcgc                               >  2:273092/1‑150 (MQ=255)
                                                                                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcgc                               >  1:723051/1‑150 (MQ=255)
                                                                                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcgc                               >  2:430868/1‑150 (MQ=255)
                                                                                                                       cccGCCCCTGACACAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCCCCATCCTTGCCAGCCATTCATATTAATACACATCATCCAGCACGTTAATTTTCAAAAGATcgcg                              <  2:168248/149‑1 (MQ=255)
                                                                                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGATGTAAATCGTTCTACCGGGATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATcgcta                             <  2:1590628/150‑3 (MQ=255)
                                                                                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGa                             >  2:443960/1‑150 (MQ=255)
                                                                                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATGCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGa                             >  2:1040358/1‑150 (MQ=255)
                                                                                                                         cGCACCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAACCTTGCCAGCCATTCATATTAATACACTTCATCCAGCAAGTTAATTTTCAAAAGATCGCGaa                            >  2:1390789/1‑150 (MQ=255)
                                                                                                                             ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGAATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCaa                        >  2:613076/1‑150 (MQ=255)
                                                                                                                               tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACg                      <  2:225575/150‑1 (MQ=255)
                                                                                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGc                     >  2:1195980/1‑150 (MQ=255)
                                                                                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTAATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGc                     >  1:138739/1‑150 (MQ=255)
                                                                                                                                   aGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAtt                  >  1:435861/1‑150 (MQ=255)
                                                                                                                                    taaaTCATCCTTAGAGAAAGCTAAGGATTTTTTTTATATGAAATAACCCTCTACGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttt                 <  1:350241/149‑1 (MQ=255)
                                                                                                                                    gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCGTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttt                 >  2:1816284/1‑150 (MQ=255)
                                                                                                                                     aaaaaatcCTTAGCTTTCGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAtttt                >  2:1830860/6‑150 (MQ=255)
                                                                                                                                      aaaaatcCTTAGCTTTCGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCAttttt               >  1:758240/5‑150 (MQ=255)
                                                                                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTa              <  1:1216408/150‑1 (MQ=255)
                                                                                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTAt             >  1:1391390/1‑150 (MQ=255)
                                                                                                                                         catcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATc            >  2:1735039/1‑150 (MQ=255)
                                                                                                                                          atcCTTAGCTTACGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCATTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACTTTAATTTTCAAAAGATCGCGAATCAACGCATGTTTATcc           >  2:283229/1‑149 (MQ=255)
                                                                                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCg           <  2:1453880/150‑1 (MQ=255)
                                                                                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGc          <  2:1550719/150‑1 (MQ=255)
                                                                                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTa        <  2:431993/150‑1 (MQ=255)
                                                                                                                                                   gAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGc  <  2:833675/150‑1 (MQ=255)
                                                                                                                                                   gAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGc  >  2:88024/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACCCTAATTAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCATCCAGCACGTTAATTTTCAAAAGATCGCGAATCAACGCATTTTTATCGCTATTTTGC  >  NC_000913/3946901‑3947197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: