Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,515 T→A 5.9% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5150TA 5.9% 303.1 / 5.2 118intergenic (+70/+17)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (50/56);  new base A (4/4);  total (58/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 119 total aligned reads displayed.

GCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACC  >  NC_000913/3959366‑3959663
                                                                                                                                                     |                                                                                                                                                    
gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTCTTGCTGTGGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                                      <  2:519207/150‑1 (MQ=255)
  aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                                                                                    <  2:1041480/150‑1 (MQ=255)
    ccATGCGCTTGAGCAGGTAGGTACGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgag                                                                                                                                                  <  1:981991/150‑1 (MQ=255)
        gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGGAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                                                              <  2:1537732/150‑1 (MQ=255)
         cGATGGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAAGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGc                                                                                                                                             <  2:563410/150‑1 (MQ=255)
           aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCt                                                                                                                                           <  2:813204/150‑1 (MQ=255)
             tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                                                         <  1:227573/150‑1 (MQ=255)
              gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                                                                                        <  2:54144/150‑1 (MQ=255)
              gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                                                                                        >  2:861876/1‑150 (MQ=255)
              gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGTAAGGGGGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgg                                                                                                                                        >  1:986899/1‑149 (MQ=39)
                gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                                                                                      <  1:501174/150‑1 (MQ=255)
                 cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAt                                                                                                                                     <  2:1565877/150‑1 (MQ=255)
                 cAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgagtg                                                                                                                                     <  2:158054/150‑5 (MQ=255)
                  cggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                                                                                    <  1:515492/149‑1 (MQ=255)
                   ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGTGAAAACACTCAAGGCCTTCTCCTGGAGAATGCCTTGGTAttt                                                                                                                                   >  1:603174/1‑149 (MQ=255)
                   ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                                                   <  1:4113/150‑1 (MQ=255)
                   ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTATCCCCCAGGGAGAGGGTGAAAACACTCAAGGCCTTCTCCGGGAGACTGCCGTGcgtata                                                                                                                                   >  1:530842/1‑145 (MQ=38)
                   ggtaggtaAGAAACTGCGCGGCTATATGACAGAGATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                                                   <  2:844932/150‑1 (MQ=255)
                   ggtaggtaAGAAACTGCGCGGCTATATGACAAATATGAAAAGTATTGATGTTGACGGTTAAGTGCGCGCTGAAGCCCTAACCCCTACACTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTAGCttatt                                                                                                                                   >  1:305430/1‑146 (MQ=37)
                    gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAg                                                                                                                                  <  1:699331/150‑1 (MQ=255)
                         taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACGGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                                                             >  2:1927380/1‑150 (MQ=255)
                          aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                                                            <  2:296235/150‑1 (MQ=255)
                           aGAAACTGCGCGGCTATATGACTGAGATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAAGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGt                                                                                                                           <  2:937771/150‑1 (MQ=255)
                            gAAACTGCGCGTCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTCCTCGAGAATGCCTTGCTATTAGTTGCGCTa                                                                                                                          >  2:1379210/1‑150 (MQ=38)
                               aCTGCGCGGCTATATGACAGATTTGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                                                                                       <  2:1508669/150‑1 (MQ=255)
                                 tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                                                                                     <  1:1408289/150‑1 (MQ=255)
                                 tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGGACAGcc                                                                                                                     >  1:1926263/1‑149 (MQ=255)
                                        cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                                                              <  1:1545575/150‑1 (MQ=255)
                                          atatGACAGATATGAAACGTATTGCTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatga                                                                                                            <  2:812241/150‑1 (MQ=255)
                                            atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                                          <  1:791847/150‑1 (MQ=255)
                                             tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACGCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                                         <  2:945953/150‑1 (MQ=255)
                                              gACAGCTATGAAACGTAGTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                                                                                        <  1:1354653/150‑1 (MQ=255)
                                              gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgtg                                                                                                        <  1:524044/150‑1 (MQ=255)
                                                      tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGTGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCGTAATGATGTGATATACGa                                                                                                >  1:1754167/1‑150 (MQ=255)
                                                       gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGCAGGGCGTGGGATTAGTTGCGGTACAGCACCGTAATGATGTGATATGCGaa                                                                                               >  2:694486/1‑150 (MQ=37)
                                                       gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTACCACAGGGAGAGGGGGAAAACACTCCAGGCCATATCCGGGAGAAGGCCGTGCTATTAGTTTCGGTACCGCACCGTAATGGTGTGATATCCGaa                                                                                               >  1:1668113/1‑150 (MQ=37)
                                                        aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGTGAAAACACAAAAGGCCTTCTCCTGGAGTAGGCCGTTCGATTTGTTGCGGTTCAGGACCGTATTGATGGGAGATGCGGAc                                                                                              >  1:404648/1‑150 (MQ=21)
                                                          aCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTg                                                                                            <  1:2011233/150‑1 (MQ=255)
                                                            gTATTGCTGTTGCGGGTAAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGTAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                                                                                          <  2:757115/150‑1 (MQ=255)
                                                             tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                                         <  1:1173100/150‑1 (MQ=255)
                                                                tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                                                      <  1:162867/150‑1 (MQ=255)
                                                                tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                                                      >  2:1443908/1‑150 (MQ=255)
                                                                      tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGTGAGGGAGAAAACACTCAAGGGCTTATCCTGGTGAAGGCCTTGCTATTAGTTTGGGTTCAGCACCTTAATTATGTGAAATACGAACTGGGTGTGCAGCgg                                                                                >  1:175225/1‑150 (MQ=37)
                                                                       gCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCggg                                                                               <  1:1717449/150‑1 (MQ=255)
                                                                             tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                                                         <  1:1259764/150‑1 (MQ=255)
                                                                              aaGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCCGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACTGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCg                                                                        <  2:1978223/150‑1 (MQ=255)
                                                                              aaGTGCGCGCTGATGCCCTCACCCCGACCCTATCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTATTTGCGGTACAGCACCATAATGATGTGATATGCGAACTGGGTGTTCAGCGGGCCGGTCg                                                                        >  1:938145/1‑150 (MQ=38)
                                                                               aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                       <  2:370794/150‑1 (MQ=255)
                                                                               aGTGCGCGCTGATGCCCTAACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTGTGCTGGAGAAGGCCTTGCTATTAGTTTCGGTACACCACCTTTATGATTTGATATACGAACAGGGTGTGCAGCGGGCCGGTCgg                                                                       >  2:446888/1‑150 (MQ=37)
                                                                                        tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc                                                              >  2:249058/1‑150 (MQ=255)
                                                                                         gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                             >  2:96681/1‑150 (MQ=255)
                                                                                         gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                             >  2:244408/1‑150 (MQ=255)
                                                                                         gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                             <  1:1413759/150‑1 (MQ=255)
                                                                                           tGCCCTCACCACGACACTCTCCCACAGGTAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGACTTGCTATTAGTTGCGGTACAGCACCTAAATGATGTGATATACGAACTGGTTGTGCAGCGGGCCGGTCGGCTCCAGTACCgg                                                           <  1:1815600/150‑1 (MQ=255)
                                                                                            gCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                                                          >  2:1472317/1‑150 (MQ=255)
                                                                                             cccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGAc                                                         <  2:1973614/150‑1 (MQ=255)
                                                                                              ccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTTCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACa                                                        >  2:1374232/1‑150 (MQ=255)
                                                                                                 cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                                     >  1:1391866/1‑150 (MQ=255)
                                                                                                 cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                                     >  1:1399896/1‑150 (MQ=255)
                                                                                                   ccccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCATCGGGCCGGTCGGCTCCAGTACCGGACAAgaga                                                   >  2:402266/1‑150 (MQ=255)
                                                                                                   ccccGACCCACTCCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATACCAACTGGGTGTGAAGCGCGCCGGTCGGCTCCAGTACCGGACAAgaga                                                   >  2:1042755/1‑150 (MQ=255)
                                                                                                       gACCCTCTCCCACTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAc                                               <  2:954206/150‑1 (MQ=255)
                                                                                                        aCCCTCTCCCACAGGGTGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCTGTACATCAGCTTAATGATTTGATTTCCGAACTGGGTGTGCAGCGGGCCGGGCGGCTCCAGTTCCGGACAAGAGAAAAcc                                              <  1:1101855/150‑1 (MQ=255)
                                                                                                         cccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAACACCa                                             >  1:27722/1‑150 (MQ=255)
                                                                                                           ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCTGCTCCAGTACCGGACAAGAGAAAACCACt                                           <  2:1785427/150‑1 (MQ=255)
                                                                                                           ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                                           >  2:475642/1‑150 (MQ=255)
                                                                                                             ctcCCAAAGGGAGAGTGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATTTCCGAACAGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACCAGAGACAACCCCttt                                         >  2:1307409/1‑150 (MQ=255)
                                                                                                               cccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCAGCTTAATGATGTGATATCCGAACAGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACAGGACAAGAGATAACCACTTTAt                                       >  1:14194/1‑150 (MQ=255)
                                                                                                               cccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAt                                       >  2:1756789/1‑150 (MQ=255)
                                                                                                               cccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTCCGGTACAGCACCTTAATGATCTGATATCCGAACTGGGTGAGCAGCGTGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAt                                       >  1:20950/1‑150 (MQ=255)
                                                                                                                ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                      <  1:1732001/150‑1 (MQ=255)
                                                                                                                 cacaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGATCTGGGTGTGAAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCg                                     >  1:91462/1‑150 (MQ=255)
                                                                                                                 cacaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCg                                     >  2:1036430/1‑150 (MQ=255)
                                                                                                                   caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa                                   >  2:956652/1‑150 (MQ=255)
                                                                                                                      ggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc                                >  1:2044241/1‑150 (MQ=255)
                                                                                                                       gagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGcc                               <  1:892263/150‑1 (MQ=255)
                                                                                                                        agagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCg                              >  2:768215/1‑150 (MQ=255)
                                                                                                                        agagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCg                              >  1:636852/1‑150 (MQ=255)
                                                                                                                           gggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa                           >  2:1674848/1‑150 (MQ=255)
                                                                                                                           gggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGaa                           >  1:1009214/1‑150 (MQ=255)
                                                                                                                            ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc                          >  1:516510/1‑150 (MQ=255)
                                                                                                                            ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc                          <  1:396983/150‑1 (MQ=255)
                                                                                                                             gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAcc                         <  2:603174/150‑1 (MQ=255)
                                                                                                                                aaaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                      >  2:687927/1‑150 (MQ=255)
                                                                                                                                aaaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                      <  1:774173/150‑1 (MQ=255)
                                                                                                                                 aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATcg                     >  1:1823915/1‑149 (MQ=255)
                                                                                                                                    cacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAc                  >  2:1828174/1‑150 (MQ=255)
                                                                                                                                      agcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGCACGCCGGAACCATCTGAccc                >  2:841419/3‑150 (MQ=255)
                                                                                                                                      agcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGACGGAACCATCTGAccc                >  2:562389/3‑150 (MQ=255)
                                                                                                                                        cAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTg              >  2:2049958/1‑150 (MQ=255)
                                                                                                                                           ggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGTTGTGCAGCGGGCCGGTCGGGTCCAGTACCGGACAAGAGAAAACCACTTTATCTAACGCCGTAACCATCTGACCCTGGCg           <  2:305430/150‑1 (MQ=255)
                                                                                                                                            gCCTACACCTGGCGAAGTCCTTGCTATTAGTGGCGGTACAGCACCTTAATGATGTGATATCCGACCGGGGGGGGCAGCGGGCCGGTCGGCGCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCgg          <  2:334765/150‑1 (MQ=255)
                                                                                                                                             ccTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGa         >  1:57560/1‑150 (MQ=255)
                                                                                                                                             ccTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGa         >  1:1984803/1‑150 (MQ=255)
                                                                                                                                              cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCATCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa        <  1:1171401/150‑1 (MQ=255)
                                                                                                                                              cTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGaa        <  1:1709509/150‑1 (MQ=255)
                                                                                                                                               ttCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGTAGCGGGCCGGTCGGCTCCAGTACCGGACAAAAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAAt       <  2:1436213/150‑1 (MQ=255)
                                                                                                                                                tctcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAAtt      <  1:557061/150‑1 (MQ=255)
                                                                                                                                                 ctcCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTc     <  1:611870/150‑1 (MQ=255)
                                                                                                                                                    cTGGAGAAGGCCTTGCTATTAGTTGAGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAcc  <  2:1531090/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACC  >  NC_000913/3959366‑3959663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: