Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,642 A→C 6.0% intergenic (‑89/+19) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,6420AC 6.0% 351.7 / 10.9 116intergenic (‑89/+19)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (62/47);  new base C (2/5);  total (64/52)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

Only 100 of 119 total aligned reads displayed.

TCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCG  >  NC_000913/556493‑556791
                                                                                                                                                     |                                                                                                                                                        
tCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGa                                                                                                                                                          >  2:1637238/1‑150 (MQ=255)
tCGAACCCAACACTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTAGa                                                                                                                                                          >  1:1945096/1‑150 (MQ=255)
  gAACCCAACCCTTAACCAATGCATTGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGTTGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                        <  1:425420/150‑1 (MQ=255)
  gAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTGGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                        >  1:763292/1‑150 (MQ=255)
  gAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGGCGCTGCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                        <  1:1836477/150‑1 (MQ=255)
   aacccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATAc                                                                                                                                                       >  1:1597194/1‑150 (MQ=255)
   aacccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGAGGAACGGGGCGTAGAGGGCAAAAGTCTGATAAGAGAACCGGCGTGTTGATAc                                                                                                                                                       >  2:1962453/1‑150 (MQ=255)
          cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCgg                                                                                                                                                <  1:1990312/150‑1 (MQ=255)
           ccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggt                                                                                                                                               <  1:573190/150‑2 (MQ=255)
           ccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggt                                                                                                                                               <  1:964500/150‑2 (MQ=255)
            cTAAACCAATGCCTAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                              <  2:1772859/150‑1 (MQ=255)
            cTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCCATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                              >  1:922979/1‑150 (MQ=255)
             tAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggttc                                                                                                                                             <  2:1905588/150‑4 (MQ=255)
             aaaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggttc                                                                                                                                             <  2:511909/149‑4 (MQ=255)
              aaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGaaa                                                                                                                                            >  1:211879/1‑150 (MQ=255)
              aaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGaaa                                                                                                                                            >  1:1837246/1‑150 (MQ=255)
               aaCCACTGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCAGCCTGTTGATACAGGCCGGGAAA‑‑‑g                                                                                                                                        <  2:1687471/150‑1 (MQ=255)
               aaCCAATGCATAGTGTAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCTTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑g                                                                                                                                        <  1:391323/150‑1 (MQ=255)
                      gCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAg                                                                                                                                 >  2:300248/1‑150 (MQ=255)
                        aTAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGTGAAA‑‑‑GGGATCAGGc                                                                                                                               >  1:1455838/1‑150 (MQ=255)
                        aTAGGGGAACGGCGGCGTGCGTAATAACGCTTGTCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGGCTGAAAAGAGAACCGGCCTGATGATACAGGCCGGGAAA‑‑‑GGGATCAGGc                                                                                                                               <  1:62575/150‑1 (MQ=255)
                                     ggcgggcgTAAAAACGGTTGGCATGATGGTCGCTTCGGCAAAAAGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCGGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACg                                                                                                                  <  1:1400792/150‑1 (MQ=255)
                                     ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACg                                                                                                                  >  1:255684/1‑150 (MQ=255)
                                     ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACg                                                                                                                  >  2:1199611/1‑150 (MQ=255)
                                      gcgtgcgtAATAACGGTTGGCATGATGGGCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGc                                                                                                                 <  1:435750/150‑1 (MQ=255)
                                       cgtgcgtAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCt                                                                                                                >  2:165915/1‑150 (MQ=255)
                                          gcgtAATAACGGGGGGCATGATGGTCGCTGCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCtgtg                                                                                                             <  2:1536276/150‑1 (MQ=255)
                                             taataaCGGTTGGCATGAAGGTCGCTTCGGCAAAATGTCGAGCCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGACTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGAcc                                                                                                          <  1:1349509/150‑1 (MQ=255)
                                                taaCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGGTGATAAAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACCCTGTGACCTGc                                                                                                       >  2:1841559/1‑150 (MQ=255)
                                                       tGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTc                                                                                                <  1:555624/150‑1 (MQ=255)
                                                        ggCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCa                                                                                               >  1:401965/1‑150 (MQ=255)
                                                          cATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACt                                                                                             <  1:28752/150‑1 (MQ=255)
                                                          cATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACt                                                                                             >  1:1108906/1‑150 (MQ=255)
                                                           atgaggGTCGCTTCGGCAAAATGGCGATTCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTg                                                                                            <  2:362406/150‑1 (MQ=255)
                                                            tgatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGt                                                                                           >  1:1124116/1‑150 (MQ=255)
                                                            tgatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGt                                                                                           >  2:962045/1‑150 (MQ=255)
                                                             gatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTc                                                                                          <  1:1264091/150‑1 (MQ=255)
                                                             gatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTc                                                                                          <  1:1051176/150‑1 (MQ=255)
                                                              ttgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGTATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCt                                                                                         <  1:1685799/149‑1 (MQ=255)
                                                               ggggcGCTGCGTCAAAATGTCGATGCTATCAGCATGGATGATCGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGAGCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTg                                                                                        <  1:2028721/146‑1 (MQ=255)
                                                                 gTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGTCTGAc                                                                                      >  2:1435457/1‑150 (MQ=255)
                                                                 gTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGAc                                                                                      <  2:1761587/150‑1 (MQ=255)
                                                                         ggCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacg                                                                              >  1:1209005/1‑150 (MQ=255)
                                                                         ggCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacg                                                                              >  1:1709337/1‑150 (MQ=255)
                                                                          gCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacga                                                                             >  2:878542/1‑150 (MQ=255)
                                                                                tGTCGATGCTATCAGCATGGATGACCGGGGCGTATAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACTACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       <  1:1188574/150‑1 (MQ=255)
                                                                                tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       <  2:877434/150‑1 (MQ=255)
                                                                                tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       >  1:616906/1‑150 (MQ=255)
                                                                                tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       <  1:1101412/150‑1 (MQ=255)
                                                                                       gCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGttt                                                                >  1:1202051/1‑150 (MQ=255)
                                                                                        cTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTg                                                               >  2:1621188/1‑150 (MQ=255)
                                                                                         tATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTTATACAAGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgc                                                              >  2:120238/1‑150 (MQ=255)
                                                                                         tATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAACACTCACTGTCTGACCGGCGACGAGTTTGCAGCGTTTgc                                                              >  1:342099/1‑150 (MQ=255)
                                                                                          aTCAGCATGTATGAACGGTGCTTAGAGTGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcg                                                             <  2:643782/150‑1 (MQ=255)
                                                                                            cAGCATGGATGAACGGGGCGTAGAGGGCATAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGTCCGGCGACGATTTTGCAGCGTTTGcgcg                                                           <  1:262878/150‑1 (MQ=255)
                                                                                            cAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                           >  2:465852/1‑150 (MQ=255)
                                                                                            cAGAATGGATGAAAGGGGCGTAGAGGGCAAAAGTGTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                           <  1:303080/150‑1 (MQ=255)
                                                                                             aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGt                                                          >  1:453814/1‑150 (MQ=255)
                                                                                             aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGAAGCGTTTGCTCGt                                                          >  2:1095247/1‑150 (MQ=255)
                                                                                             aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCAGCAAAACTCACTGTCTGACCGGCGAAGATTTTGCAGCGTTTTCGCGt                                                          >  2:1557725/1‑150 (MQ=255)
                                                                                               cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                        <  1:958691/150‑1 (MQ=255)
                                                                                               cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                        >  2:106511/1‑150 (MQ=255)
                                                                                               cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCAGCAAAACTCACTGTCTGACCCGCGACGATTTTGCCGGGTTTGCGCGttt                                                        >  2:1570764/1‑150 (MQ=255)
                                                                                                 tGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTcc                                                      >  1:1435677/1‑149 (MQ=255)
                                                                                                  ggATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCaa                                                     <  2:252111/150‑1 (MQ=255)
                                                                                                     tGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCg                                                  >  1:787514/1‑150 (MQ=255)
                                                                                                      gAACGGGGCGTATAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAT‑‑‑GGGATAAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGCCGATTTTGCAGCGTTTGCGCGTTTCAACCGc                                                 >  2:1282326/1‑150 (MQ=255)
                                                                                                       aaCGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACGCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCa                                                >  1:247128/1‑150 (MQ=255)
                                                                                                            ggCGTAGAGGGCAATAGGCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTc                                           <  2:1687038/150‑1 (MQ=255)
                                                                                                            ggCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTc                                           >  1:427087/1‑150 (MQ=255)
                                                                                                                tAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACt                                       <  1:1887468/150‑1 (MQ=255)
                                                                                                                    gggCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCa                                   >  2:1705451/1‑150 (MQ=255)
                                                                                                                      gCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAcc                                 <  2:1297783/150‑1 (MQ=255)
                                                                                                                       cAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAAACGGAATGAGGCTACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGAGGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCa                                   >  2:314454/1‑150 (MQ=255)
                                                                                                                          aactCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGc                             <  1:1638657/147‑1 (MQ=255)
                                                                                                                            gTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCt                           <  1:1557726/150‑1 (MQ=255)
                                                                                                                               tGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCg                        >  1:905370/1‑150 (MQ=255)
                                                                                                                                 aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  1:221151/150‑1 (MQ=255)
                                                                                                                                 aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      >  2:1713145/1‑150 (MQ=255)
                                                                                                                                 aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  1:176135/150‑1 (MQ=255)
                                                                                                                                 aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGACTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  2:255684/150‑1 (MQ=255)
                                                                                                                                 aaaaGAGAACAGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  1:1269479/150‑1 (MQ=255)
                                                                                                                                   aaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCaa                    >  1:60560/1‑150 (MQ=255)
                                                                                                                                     gagaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCCGCGTTTGCGCGTTTCAACCGCACCGTCGGCGTTCACCTGGCCGTCGGCAATc                  >  2:1301428/1‑150 (MQ=255)
                                                                                                                                     gagaACCGGCCTGTTGAAACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCCCCGTCGACTTTCACCTGGCCTTCGGCAATc                  >  2:858701/1‑150 (MQ=255)
                                                                                                                                      agaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCATTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcg                 >  2:2017309/1‑150 (MQ=255)
                                                                                                                                       gaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcgc                >  1:383223/1‑150 (MQ=255)
                                                                                                                                        aaCCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCATCAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGAGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACGGTCGACTTTCACCTGGCCTTCGGCAATcgcg               >  1:1291287/1‑150 (MQ=255)
                                                                                                                                          ccGGCCTGTATCAACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt             >  1:538446/1‑150 (MQ=255)
                                                                                                                                          ccGGCCTGTATCAACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt             >  2:1188240/1‑150 (MQ=255)
                                                                                                                                            ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           >  1:1091021/1‑150 (MQ=255)
                                                                                                                                            ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           <  1:1825180/150‑1 (MQ=255)
                                                                                                                                            ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           >  2:1132865/1‑150 (MQ=255)
                                                                                                                                             gCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAtttt          >  1:1402250/1‑150 (MQ=255)
                                                                                                                                                tGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc       >  1:46725/1‑150 (MQ=255)
                                                                                                                                                 gTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc      <  2:453814/150‑1 (MQ=255)
                                                                                                                                                 gTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc      <  2:1311332/150‑1 (MQ=255)
                                                                                                                                                    gATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc   >  1:451300/1‑150 (MQ=255)
                                                                                                                                                    gATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc   >  2:78646/1‑150 (MQ=255)
                                                                                                                                                     aTACAGGCCGGGTAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAATCTCACTGTCTGACCGGCGACGATTTTGCAGCGGTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcg  <  2:732220/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCG  >  NC_000913/556493‑556791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: