Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,094,415 T→G 6.6% intergenic (‑143/+308) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,094,4150TG 6.6% 380.7 / 10.3 146intergenic (‑143/+308)rhaD/rhaArhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base T (62/73);  new base G (9/2);  total (71/75)
Fisher's exact test for biased strand distribution p-value = 2.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

TTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTC  >  NC_000913/4094266‑4094563
                                                                                                                                                     |                                                                                                                                                    
tttGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCGCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCt                                                                                                                                                      <  2:618883/150‑1 (MQ=255)
 ttGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTg                                                                                                                                                     <  1:368805/150‑1 (MQ=255)
 ttGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTg                                                                                                                                                     <  1:1675469/150‑1 (MQ=255)
 ttGCATGTGCTGTGTACCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTg                                                                                                                                                     <  2:1800850/150‑1 (MQ=255)
  tGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGa                                                                                                                                                    >  2:1131587/1‑150 (MQ=255)
  tGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGa                                                                                                                                                    <  1:1807303/150‑1 (MQ=255)
   gCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGAt                                                                                                                                                   >  2:1115320/1‑150 (MQ=255)
   gCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGAt                                                                                                                                                   <  1:145686/150‑1 (MQ=255)
   gCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCAGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGAt                                                                                                                                                   <  2:437034/150‑1 (MQ=255)
    cATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATa                                                                                                                                                  >  2:630865/1‑150 (MQ=255)
        ttCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGc                                                                                                                                              >  1:1268781/1‑150 (MQ=255)
            gtgtTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACtt                                                                                                                                          >  1:1913582/1‑150 (MQ=255)
                tCCTGTACATTCGGTGTTGCCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAAAGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGc                                                                                                                                      <  1:1329606/150‑1 (MQ=255)
                 ccTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGcc                                                                                                                                     >  2:1033653/1‑150 (MQ=255)
                   tGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCgt                                                                                                                                   <  1:559673/150‑2 (MQ=255)
                    gTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCATGATAAGGCACTTGTGCCGCa                                                                                                                                  >  2:251446/1‑150 (MQ=255)
                        aTTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCg                                                                                                                              >  1:1231437/1‑150 (MQ=255)
                        aTTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCg                                                                                                                              >  2:728499/1‑150 (MQ=255)
                          tCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGc                                                                                                                            <  2:1618356/150‑1 (MQ=255)
                          tCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGc                                                                                                                            <  1:2019072/150‑1 (MQ=255)
                          tCGGTGGTGTCGGATGCACGACCCGTAGGCCGGTTAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGc                                                                                                                            <  2:237706/150‑1 (MQ=255)
                              tgttgtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaatc                                                                                                                        <  2:1098413/150‑1 (MQ=255)
                              ggttgtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaatc                                                                                                                        <  1:645388/149‑1 (MQ=255)
                               gttgtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGcaatca                                                                                                                       <  2:1442702/150‑1 (MQ=255)
                                 tgtCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCAGGcatt                                                                                                                         >  2:1355162/1‑144 (MQ=255)
                                    cGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGcc                                                                                                                  <  1:1368683/150‑1 (MQ=255)
                                      gATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTg                                                                                                                <  2:1015507/150‑1 (MQ=255)
                                       aTGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGa                                                                                                               <  1:554644/150‑1 (MQ=255)
                                         gCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATg                                                                                                             <  2:1756964/150‑1 (MQ=255)
                                          cACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACTCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc                                                                                                            <  2:1308491/150‑1 (MQ=255)
                                          cACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACACCTATTGCCGACCTGTAGGCCGGATAAGGGACTTGTGCCGCATCCGGCACTCAATGCCTGATGc                                                                                                            >  1:136217/1‑150 (MQ=255)
                                          cACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc                                                                                                            <  1:1466001/150‑1 (MQ=255)
                                          aaCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGc                                                                                                            <  1:1535403/149‑1 (MQ=255)
                                             gACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGAc                                                                                                         >  2:1192067/1‑150 (MQ=255)
                                              aCCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAGCTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACg                                                                                                        >  1:271386/1‑150 (MQ=255)
                                               cccGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGc                                                                                                       <  2:156113/150‑1 (MQ=255)
                                                    aGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg                                                                                                  <  1:104925/150‑1 (MQ=39)
                                                         ggATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACTCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt                                                                                             <  2:1363647/150‑1 (MQ=255)
                                                         ggATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt                                                                                             >  1:1154141/1‑150 (MQ=21)
                                                         ggATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCt                                                                                             >  2:868152/1‑150 (MQ=21)
                                                          gATTAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCtt                                                                                            <  1:2040655/150‑1 (MQ=255)
                                                          gATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCGTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCtt                                                                                            <  2:322821/150‑1 (MQ=255)
                                                           aTAAGGCGCTCGCGCCGCATCCGGCAATCCATGCCTGCTGCGACGCGGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATGAGGCACTTGTGCCGCATCCGGCAATCACTGCCTGATGCGACGCTGTCGCGTCTTa                                                                                           <  1:539581/150‑1 (MQ=14)
                                                              aGGCGCTCGCGCCGCATCCGGCAATCAATACCTGATGCGACGATGTCGCGTCTTATCAGGCCTAAAACTATTTCCGCCCTGTAGGCCTGATACGGCACTTGAGCCGCATCCCGCAATCAATGCCTGATGCGACGCTGTCGCGGCGTATCa                                                                                        >  2:377354/1‑150 (MQ=14)
                                                               ggCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAg                                                                                       <  1:26511/150‑1 (MQ=39)
                                                               ggCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAg                                                                                       >  2:800468/1‑150 (MQ=39)
                                                               ggCGCGCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAg                                                                                       <  2:1452661/150‑1 (MQ=37)
                                                                gcgcTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAgg                                                                                      <  1:1884368/150‑1 (MQ=38)
                                                                  gcTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGcc                                                                                    <  1:656613/150‑1 (MQ=37)
                                                                    tCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTa                                                                                  >  2:874378/1‑150 (MQ=35)
                                                                      gcgcCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATGAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGCCGCTGTCGCGGCTTATCAGGCGTAcc                                                                                >  1:1039907/1‑149 (MQ=255)
                                                                       cgccgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACaa                                                                               <  1:1018976/150‑1 (MQ=32)
                                                                        gtcgcATCAGGCATTGATTGCCGGATGCGGCGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACacc                                                                              >  1:716419/3‑148 (MQ=11)
                                                                         ccgcATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACt                                                                             >  2:392014/1‑150 (MQ=32)
                                                                             aTCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTg                                                                         >  1:1348563/1‑150 (MQ=32)
                                                                               ccGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGcc                                                                       <  2:2036609/150‑1 (MQ=32)
                                                                                   caatcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGAcc                                                                   <  2:11392/150‑1 (MQ=32)
                                                                                     atcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTg                                                                 <  2:1268780/150‑1 (MQ=33)
                                                                                      tcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGt                                                                <  2:1251215/150‑1 (MQ=34)
                                                                                      tcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGt                                                                >  2:929105/1‑150 (MQ=17)
                                                                                       caatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTa                                                               <  2:1913581/150‑1 (MQ=34)
                                                                                            ccTGATGCGACGCTGTCGCTTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCg                                                          <  2:79563/150‑1 (MQ=21)
                                                                                            ccTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCg                                                          >  1:174583/1‑150 (MQ=34)
                                                                                                aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATa                                                      <  1:814711/150‑1 (MQ=34)
                                                                                                  gCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAg                                                    >  1:915681/1‑150 (MQ=34)
                                                                                                         tGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTtg                                             <  2:1231436/150‑1 (MQ=37)
                                                                                                          gTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTtgt                                            <  2:739741/150‑1 (MQ=37)
                                                                                                               gTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGc                                       >  1:1564208/1‑150 (MQ=37)
                                                                                                                tCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCa                                      <  2:528303/150‑1 (MQ=37)
                                                                                                                   tATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg                                                                                                  >  2:362581/1‑87 (MQ=33)
                                                                                                                    aTCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACAAGTGCCTTATCCg                                  <  2:2020381/150‑1 (MQ=255)
                                                                                                                      cAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGc                                <  2:1373093/150‑1 (MQ=37)
                                                                                                                      cAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGc                                >  2:1611846/1‑150 (MQ=37)
                                                                                                                       aGGCCTACAACTATTCCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATGCGGCACAAGTGCCTTATCCGGcc                               <  1:1158259/150‑2 (MQ=11)
                                                                                                                          ccTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatc                            >  2:1965209/1‑150 (MQ=37)
                                                                                                                          ccTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatc                            >  2:1437559/1‑150 (MQ=37)
                                                                                                                            tACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatcaa                          >  2:571770/1‑150 (MQ=17)
                                                                                                                             aCAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGcaatcaat                         >  2:1773199/1‑150 (MQ=37)
                                                                                                                               aaCTATTCCCGACCTGTAGCCCTGATAAGGCACTTGAGCCGCAACCGGCAATCAATGCCTGATGCGACGCTGTCGCGCCAAATCAGGCCTACAACTCTTGCCGACCTGTAGGCCGGATAAGGCACTTGAGCCGCATCCGGCAATCAATGc                       <  1:336166/150‑1 (MQ=11)
                                                                                                                               aaCAATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTAGTGCCGCATCCGGCAATCAATGc                       <  1:340665/150‑1 (MQ=25)
                                                                                                                                 cTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCt                     <  2:915208/150‑1 (MQ=37)
                                                                                                                                 cTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCt                     <  2:285916/150‑1 (MQ=37)
                                                                                                                                 cTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCt                     <  2:1348562/150‑1 (MQ=37)
                                                                                                                                     tGCCGACCTGTCGGCCTGATGAGGCACTTGTGCCGCATCCGGCAAGCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATg                 <  2:322521/150‑1 (MQ=255)
                                                                                                                                      gCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATTCCTGATGc                >  2:1866173/1‑150 (MQ=21)
                                                                                                                                       ccGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACATGTGCCGCATCCGGCAATCAATGCCTGATGCg               >  2:189437/1‑150 (MQ=21)
                                                                                                                                        cGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGa              >  1:1602242/1‑150 (MQ=34)
                                                                                                                                         gACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGAc             >  2:572930/1‑150 (MQ=34)
                                                                                                                                           ccTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGc           >  1:1298777/1‑150 (MQ=34)
                                                                                                                                            cTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGCCCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGca          >  2:291949/1‑149 (MQ=21)
                                                                                                                                             tGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg         >  2:52766/1‑150 (MQ=34)
                                                                                                                                             tGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg         >  1:1208241/1‑150 (MQ=34)
                                                                                                                                              gTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGt        >  2:575017/1‑150 (MQ=34)
                                                                                                                                               tAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTc       >  1:496380/1‑150 (MQ=34)
                                                                                                                                                aGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCACATCCGGCAATCAATGCCTGATGCGACGCTGTcg      >  2:2026444/1‑150 (MQ=21)
                                                                                                                                                 ggCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGCCCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgc     >  2:562902/1‑150 (MQ=21)
                                                                                                                                                 agCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgc     >  1:410800/2‑150 (MQ=21)
                                                                                                                                                    cTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCg                                                                                                           >  2:334879/1‑45 (MQ=35)
                                                                                                                                                    cTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTc  >  1:917931/1‑150 (MQ=33)
                                                                                                                                                    atGATAAGGCTCTTGTGCCGCATCCGGCATTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGtt  <  1:251446/149‑2 (MQ=12)
                                                                                                                                                     |                                                                                                                                                    
TTTGCATGTTCTGTGTTCCTGTAAATTCGGTGTTGTCGGATGCACGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCTGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTC  >  NC_000913/4094266‑4094563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: