Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,303,796 G→A 5.1% T105I (ACC→ATC)  mdtN ← membrane fusion protein of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,303,7960GA 5.1% 371.8 / 5.9 116T105I (ACC→ATC) mdtNmembrane fusion protein of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (57/53);  new base A (3/3);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

Only 100 of 122 total aligned reads displayed.

CAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTG  >  NC_000913/4303647‑4303945
                                                                                                                                                     |                                                                                                                                                     
  aCATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTg                                                                                                                                                     <  1:64603/150‑1 (MQ=255)
    aTCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAg                                                                                                                                                   >  2:627216/1‑150 (MQ=255)
    aTCTTCCGCTGAGACAAAACCATCTTGCCGTAATGGCTCGGTGCGGCGTACTGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTGTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAg                                                                                                                                                   <  1:410587/150‑1 (MQ=255)
     tCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGc                                                                                                                                                  >  2:1398647/1‑150 (MQ=255)
     tCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGc                                                                                                                                                  >  1:71402/1‑150 (MQ=255)
          cGCTGAGACACAACCTTCTTTCAGTAAGGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGTGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAt                                                                                                                                             <  1:394690/150‑1 (MQ=255)
               agaCAAAACCTTCTTTCATAAATTGCTAGGTGCGACGTAATGAATCTGGGGCCTGGTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATATGCt                                                                                                                                        <  2:137632/150‑1 (MQ=255)
               agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                                                                        <  1:1966096/150‑1 (MQ=255)
               agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                                                                        <  2:1640112/150‑1 (MQ=255)
               agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                                                                        <  2:1436164/150‑1 (MQ=255)
                gaCAAAACCTTCTTTCAGTACTGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCtt                                                                                                                                       <  2:1527485/150‑1 (MQ=255)
                  cAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTAt                                                                                                                                     >  1:1619852/1‑150 (MQ=255)
                  cAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTATTCTACCGTGGCATTAACCGAGTCGGAACCAAACTTTTGCGAGTCAACGCTACGCTGGGTGAGCATAATTAGCtaaa                                                                                                                                     >  1:879766/1‑147 (MQ=255)
                     aaCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAATCTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCa                                                                                                                                  <  1:162662/150‑1 (MQ=255)
                        cttcttTCAGTAATGGCTCGGTGCGTCGTAATGTATCTGTGGCCTGTTTCGCGGAGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAgcg                                                                                                                               <  1:1152064/150‑1 (MQ=255)
                        cttcttTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTACCCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAgcg                                                                                                                               >  2:1280260/1‑150 (MQ=255)
                           cttTCAGTAATGGCGCGGTGCGGCGTAATGTATCTGTGGGCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGAACCAAACTGTTGCGCGTCAACGCTACGCTGGATAAGCAAATTATGCTCACCCAgcgtag                                                                                                                            <  2:1820025/150‑4 (MQ=255)
                            tttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGTACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGcgccgc                                                                                                                           >  2:1686405/1‑150 (MQ=255)
                            tttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGATAAGCAAATTATGCTCACCCAgcgtagc                                                                                                                           <  2:596809/150‑5 (MQ=255)
                            tttCAGTAATGGCTCGGTGCGGCGTAAGGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGATAAGCAAATTATGCTCACCCAgcgtagc                                                                                                                           <  1:214756/150‑5 (MQ=255)
                                 gTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgaggg                                                                                                                      >  2:1012383/1‑150 (MQ=255)
                                 gTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgaggg                                                                                                                      >  2:951798/1‑150 (MQ=255)
                                   aaTGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAATCAGTTGCGAGTCAACGCTACGCTGCGTGAGCATAATTTGCTTATCCATCGCCGCgagggag                                                                                                                    >  2:282457/1‑150 (MQ=255)
                                   aaTGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgagggag                                                                                                                    <  1:911203/150‑1 (MQ=255)
                                          cGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAg                                                                                                             <  2:1488372/150‑1 (MQ=255)
                                          cGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAg                                                                                                             <  1:27811/150‑1 (MQ=255)
                                             tGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCtt                                                                                                          >  1:1244798/1‑150 (MQ=255)
                                                    tAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAg                                                                                                   <  2:1383498/150‑1 (MQ=255)
                                                      aTGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAt                                                                                                 <  1:1594292/150‑1 (MQ=255)
                                                       tGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAtt                                                                                                >  1:1942285/1‑150 (MQ=255)
                                                        gTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTg                                                                                               >  1:1723538/1‑150 (MQ=255)
                                                        gTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTg                                                                                               <  2:289429/150‑1 (MQ=255)
                                                          aTCTGTGGCCTGTTTCGCGGCTGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGc                                                                                             <  2:1838559/150‑1 (MQ=255)
                                                          aTCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACAGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGc                                                                                             >  1:592649/1‑150 (MQ=255)
                                                           tCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTATCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGGATAATTTGCTTATCCAGCGCCGCGAGGGAGGATTCCGCTTTCGCCAGATTGGcg                                                                                            >  2:1174217/1‑149 (MQ=255)
                                                             tgtgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTc                                                                                          >  2:1723222/1‑150 (MQ=255)
                                                              gtgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGGCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCg                                                                                         <  1:549277/150‑1 (MQ=255)
                                                              ggggCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCg                                                                                         <  1:1566440/148‑1 (MQ=255)
                                                                         cgcgGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCgg                                                                              >  1:892303/1‑150 (MQ=255)
                                                                          gcggcgGCACGGGCTTTTTCTACCGTGGCATTACCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCggg                                                                             <  1:1495592/150‑1 (MQ=255)
                                                                           cggcggCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGt                                                                            >  1:6593/1‑150 (MQ=255)
                                                                              cggcACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCATATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCTCGGGTCGa                                                                         >  2:1903268/1‑150 (MQ=255)
                                                                                 cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                      >  1:1073806/1‑150 (MQ=255)
                                                                                 cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                      <  1:800349/150‑1 (MQ=255)
                                                                                 cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                      <  1:1344652/150‑1 (MQ=255)
                                                                                 cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGATTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                      >  2:1874971/1‑150 (MQ=255)
                                                                                 cACGGGCTTTTTCTACCGGGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCTCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                      <  1:1753971/150‑1 (MQ=255)
                                                                                  aCGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCg                                                                     <  1:455704/150‑1 (MQ=255)
                                                                                  aCGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCg                                                                     <  1:38265/150‑1 (MQ=255)
                                                                                      gCTGTTTCTACCGTGGCATTAACCGAGGCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAGTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAAc                                                                 <  1:54942/150‑1 (MQ=255)
                                                                                       cttgatCTACCGTGGAATTAACCGAGTCGGCACCAAACTGGTGCGCGGCAACGCGACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACa                                                                <  1:1272679/145‑1 (MQ=255)
                                                                                       cTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACa                                                                <  2:1314842/150‑1 (MQ=255)
                                                                                         ttttCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGc                                                              <  1:664407/150‑1 (MQ=255)
                                                                                               aCCGTGGCATTAACCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGGACGGGCGCGGGTCGATGCGGAACAGCAAATCg                                                        >  1:857034/1‑150 (MQ=255)
                                                                                                 cGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGcc                                                      >  1:1044026/1‑150 (MQ=255)
                                                                                                  gTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCACATCGccc                                                     >  2:934607/1‑150 (MQ=255)
                                                                                                  gTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGccc                                                     <  1:943021/150‑1 (MQ=255)
                                                                                                    ggCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg                                                   >  1:1133577/1‑150 (MQ=255)
                                                                                                    ggCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg                                                   <  1:1398648/150‑1 (MQ=255)
                                                                                                    ggCATTAACCGAGTCGGCACCAAACGGTTGCGCGGCAACGCTACGCTGTGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg                                                   <  1:261086/150‑1 (MQ=255)
                                                                                                     gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt                                                  >  1:1039141/1‑150 (MQ=255)
                                                                                                     gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt                                                  <  1:2030759/150‑1 (MQ=255)
                                                                                                      cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt                                                 >  2:795222/1‑150 (MQ=255)
                                                                                                      cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt                                                 >  2:788090/1‑150 (MQ=255)
                                                                                                      cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt                                                 <  2:54737/150‑1 (MQ=255)
                                                                                                       aTTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGttt                                                >  2:189716/1‑150 (MQ=255)
                                                                                                          aaCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGAc                                             <  2:1596451/150‑1 (MQ=255)
                                                                                                          aaCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGAc                                             >  2:558764/1‑150 (MQ=255)
                                                                                                          aaCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAATTGCTTATCCAGCGCCGCGAGGGAGGCATCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGACCAGCAAATCGCCCTGTTTGAc                                             >  1:1822905/1‑150 (MQ=255)
                                                                                                            ccGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTg                                           <  2:1781786/150‑1 (MQ=255)
                                                                                                             cGAGTCGGCTCCAAACTGTTGCGCGTCAACGCTACGCTGGGTTAGCATAATTTGATTATCCAGCGCCGCGAGTGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGc                                          <  2:359537/150‑1 (MQ=255)
                                                                                                                gTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTAGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTg                                       >  2:499005/1‑150 (MQ=255)
                                                                                                                gTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGAGCGCAAAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCATTCGCCAGATTGGCTTCGTACGGGCGCGTCTCGATGCGGAACAGCAACTCGACCTGTTTGACTGCCTg                                       >  1:1140463/1‑150 (MQ=255)
                                                                                                                     cACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGt                                  >  1:1568763/1‑150 (MQ=255)
                                                                                                                      aCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTc                                 <  2:71402/150‑1 (MQ=255)
                                                                                                                      aCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGCACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTc                                 >  1:485946/1‑150 (MQ=255)
                                                                                                                       ccAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCg                                >  2:1009683/1‑150 (MQ=255)
                                                                                                                        cAACCTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCTGCTTTCGCCAGATTGGCTTCGTACGGGAGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCgg                               <  2:1789008/150‑1 (MQ=255)
                                                                                                                        cAAACTGTTGCGCGGCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCgg                               <  1:1482690/150‑1 (MQ=255)
                                                                                                                         aaaCTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTGCGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGt                              >  1:339412/1‑150 (MQ=255)
                                                                                                                            cTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGAc                           <  2:694376/150‑1 (MQ=255)
                                                                                                                              gTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCg                         <  1:1828788/150‑1 (MQ=255)
                                                                                                                                tGCTCGTCAACGCTACGCTGTGTGAGCATAATTTGCTTATCCAGCGCATCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGTTCGTTGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCTGTGACCGcc                       <  2:879765/150‑1 (MQ=255)
                                                                                                                                tGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGcc                       <  2:719553/150‑1 (MQ=255)
                                                                                                                                tGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGcc                       >  1:359822/1‑150 (MQ=255)
                                                                                                                                  cgcgTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAg                     >  1:658402/1‑150 (MQ=255)
                                                                                                                                     gTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTc                  >  1:1649453/1‑150 (MQ=255)
                                                                                                                                       cAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTa                >  1:291291/1‑150 (MQ=255)
                                                                                                                                         acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACa              >  1:902137/1‑150 (MQ=255)
                                                                                                                                          cgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACaa             >  1:1151808/1‑150 (MQ=255)
                                                                                                                                            ctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATg           >  2:1413810/1‑150 (MQ=255)
                                                                                                                                              gcgctGGATAAGCAAATTATGCTCACCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCg         >  1:1158380/2‑150 (MQ=255)
                                                                                                                                              acgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGCCCGCCAGTTCTACAATGCg         >  1:1764649/1‑150 (MQ=255)
                                                                                                                                               cgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCACTGTTTGCCTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCgg        >  2:263812/1‑150 (MQ=255)
                                                                                                                                                gctGGGTGAGCATAATTTTCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGc       <  1:1027311/150‑1 (MQ=255)
                                                                                                                                                 ctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGcc      >  1:1930510/1‑150 (MQ=255)
                                                                                                                                                 ctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCACTTCTACAATGCGGcc      >  2:1309021/1‑150 (MQ=255)
                                                                                                                                                   gggTGAGCATAAGTTGCGTATCCAGCGCCGCGAGGGAGGCGTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGCCGATGCGGAACAGCAAATCGCCCTGTTTTACTGCCTGGGTGTCGGTGACCGCCAGTTCTACAATGCGGCCGc    <  1:1290140/150‑1 (MQ=255)
                                                                                                                                                     gTGAGCATACTTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTg  <  2:841704/150‑1 (MQ=255)
                                                                                                                                                     gTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTg  <  1:1089202/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTG  >  NC_000913/4303647‑4303945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: