Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,643 T→A 6.5% intergenic (‑90/+18) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,6430TA 6.5% 340.4 / 9.9 117intergenic (‑90/+18)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (61/48);  new base A (3/5);  total (64/53)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 117 total aligned reads displayed.

GAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCG  >  NC_000913/556495‑556791
                                                                                                                                                    |                                                                                                                                                       
gAACCCAACCCTTAACCAATGCATTGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGTTGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                        <  1:425420/150‑1 (MQ=255)
gAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTGGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATa                                                                                                                                                        >  1:763292/1‑150 (MQ=255)
 aacccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATAc                                                                                                                                                       <  2:1645780/150‑1 (MQ=255)
        cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCgg                                                                                                                                                <  1:1990312/150‑1 (MQ=255)
         ccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggt                                                                                                                                               <  1:573190/150‑2 (MQ=255)
         ccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggt                                                                                                                                               <  1:964500/150‑2 (MQ=255)
          cTAAACCAATGCCTAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                              <  2:1772859/150‑1 (MQ=255)
          cTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCCATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                              >  1:922979/1‑150 (MQ=255)
           tAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggttc                                                                                                                                             <  2:1905588/150‑4 (MQ=255)
           aaaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCggttc                                                                                                                                             <  2:511909/149‑4 (MQ=255)
            aaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGaaa                                                                                                                                            >  1:211879/1‑150 (MQ=255)
            aaaCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGaaa                                                                                                                                            >  1:1837246/1‑150 (MQ=255)
             aaCCACTGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCAGCCTGTTGATACAGGCCGGGAAA‑‑‑g                                                                                                                                        <  2:1687471/150‑1 (MQ=255)
             aaCCAATGCATAGTGTAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCTTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑g                                                                                                                                        <  1:391323/150‑1 (MQ=255)
              aCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑gg                                                                                                                                       >  2:176135/1‑150 (MQ=255)
                   tGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCa                                                                                                                                  >  1:254363/1‑150 (MQ=255)
                    gCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAg                                                                                                                                 >  2:300248/1‑150 (MQ=255)
                      aTAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGTGAAA‑‑‑GGGATCAGGc                                                                                                                               >  1:1455838/1‑150 (MQ=255)
                      aTAGGGGAACGGCGGCGTGCGTAATAACGCTTGTCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGGCTGAAAAGAGAACCGGCCTGATGATACAGGCCGGGAAA‑‑‑GGGATCAGGc                                                                                                                               <  1:62575/150‑1 (MQ=255)
                           ggAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGcaacaa                                                                                                                          >  1:1297784/1‑150 (MQ=255)
                                   ggcgggcgTAAAAACGGTTGGCATGATGGTCGCTTCGGCAAAAAGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCGGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACg                                                                                                                  <  1:1400792/150‑1 (MQ=255)
                                   ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACg                                                                                                                  >  1:255684/1‑150 (MQ=255)
                                   ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACg                                                                                                                  >  2:1199611/1‑150 (MQ=255)
                                     cgtgcgtAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCt                                                                                                                >  2:165915/1‑150 (MQ=255)
                                     cctgcgtAAAAACGGTTGGCATGATGGTAGCGTCGGCAAAAGGTCGATGCTATCAGCATGGCGGAACGGGGCGGAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCt                                                                                                                <  2:125456/148‑1 (MQ=255)
                                        gcgtAATAACGGGGGGCATGATGGTCGCTGCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCtgtg                                                                                                             <  2:1536276/150‑1 (MQ=255)
                                              taaCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGGTGATAAAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACCCTGTGACCTGc                                                                                                       >  2:1841559/1‑150 (MQ=255)
                                                     tGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTc                                                                                                <  1:555624/150‑1 (MQ=255)
                                                      ggCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCa                                                                                               >  1:401965/1‑150 (MQ=255)
                                                       gCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGTCGTAGAGTGCAAAAGTCTGAAACGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCAc                                                                                              <  2:1039431/150‑1 (MQ=255)
                                                        cATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACt                                                                                             >  1:1108906/1‑150 (MQ=255)
                                                        cATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACt                                                                                             <  1:28752/150‑1 (MQ=255)
                                                          tgatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGt                                                                                           >  1:1124116/1‑150 (MQ=255)
                                                          tgatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGt                                                                                           >  2:962045/1‑150 (MQ=255)
                                                           gatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTc                                                                                          <  1:1051176/150‑1 (MQ=255)
                                                           gatgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTc                                                                                          <  1:1264091/150‑1 (MQ=255)
                                                            ttgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGTATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCt                                                                                         <  1:1685799/149‑1 (MQ=255)
                                                             ggggcGCTGCGTCAAAATGTCGATGCTATCAGCATGGATGATCGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGAGCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTg                                                                                        <  1:2028721/146‑1 (MQ=255)
                                                               gTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGTCTGAc                                                                                      >  2:1435457/1‑150 (MQ=255)
                                                               gTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGAc                                                                                      <  2:1761587/150‑1 (MQ=255)
                                                                       ggCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacg                                                                              >  1:1209005/1‑150 (MQ=255)
                                                                       ggCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacg                                                                              >  1:1709337/1‑150 (MQ=255)
                                                                        gCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgacga                                                                             >  2:878542/1‑150 (MQ=255)
                                                                              tGTCGATGCTATCAGCATGGATGACCGGGGCGTATAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACTACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       <  1:1188574/150‑1 (MQ=255)
                                                                              tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       >  1:616906/1‑150 (MQ=255)
                                                                              tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       <  2:877434/150‑1 (MQ=255)
                                                                              tGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGc                                                                       <  1:1101412/150‑1 (MQ=255)
                                                                                     gCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGttt                                                                >  1:1202051/1‑150 (MQ=255)
                                                                                      cTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGTAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGCACGGCGCCGATTTTGCAGCGTTTg                                                               >  1:363813/1‑150 (MQ=255)
                                                                                      cTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTg                                                               >  2:1621188/1‑150 (MQ=255)
                                                                                       tATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTTATACAAGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgc                                                              >  2:120238/1‑150 (MQ=255)
                                                                                        aTCAGCATGTATGAACGGTGCTTAGAGTGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcg                                                             <  2:643782/150‑1 (MQ=255)
                                                                                         tCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGAAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcgc                                                            >  2:445131/1‑150 (MQ=255)
                                                                                          cAGCATGGATGAACGGGGCGTAGAGGGCATAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGTCCGGCGACGATTTTGCAGCGTTTGcgcg                                                           <  1:262878/150‑1 (MQ=255)
                                                                                          cAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                           >  2:465852/1‑150 (MQ=255)
                                                                                          cAGAATGGATGAAAGGGGCGTAGAGGGCAAAAGTGTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGcgcg                                                           <  1:303080/150‑1 (MQ=255)
                                                                                           aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGt                                                          >  1:453814/1‑150 (MQ=255)
                                                                                           aGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGAAGCGTTTGCTCGt                                                          >  2:1095247/1‑150 (MQ=255)
                                                                                             cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                        <  1:958691/150‑1 (MQ=255)
                                                                                             cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCAGCAAAACTCACTGTCTGACCCGCGACGATTTTGCCGGGTTTGCGCGttt                                                        >  2:1570764/1‑150 (MQ=255)
                                                                                               tGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTcc                                                      >  1:1435677/1‑149 (MQ=255)
                                                                                                ggATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCaa                                                     <  2:252111/150‑1 (MQ=255)
                                                                                                   tGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCg                                                  >  1:787514/1‑150 (MQ=255)
                                                                                                   tGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCg                                                  <  1:438568/150‑1 (MQ=255)
                                                                                                     aaCGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACGCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCa                                                >  1:247128/1‑150 (MQ=255)
                                                                                                          ggCGTAGAGGGCAATAGGCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTc                                           <  2:1687038/150‑1 (MQ=255)
                                                                                                          ggCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTc                                           >  1:427087/1‑150 (MQ=255)
                                                                                                              tAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACt                                       <  1:1887468/150‑1 (MQ=255)
                                                                                                              tAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGAATTTGCAGCGTTTGCGCGTTTCAACCGCCCCGTCGagg                                       >  1:1968342/1‑148 (MQ=255)
                                                                                                                   ggCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAc                                  >  1:1821174/1‑150 (MQ=255)
                                                                                                                    gCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAcc                                 <  2:1297783/150‑1 (MQ=255)
                                                                                                                     cAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAAACGGAATGAGGCTACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGAGGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCa                                   >  2:314454/1‑150 (MQ=255)
                                                                                                                        aactCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGc                             <  1:1638657/147‑1 (MQ=255)
                                                                                                                        aaGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGc                             <  2:1485225/150‑1 (MQ=255)
                                                                                                                          gTCTGAAAAGAGAACCGGCCGGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCt                           <  1:1557726/150‑1 (MQ=255)
                                                                                                                             tGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCg                        >  1:905370/1‑150 (MQ=255)
                                                                                                                               aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  1:176135/150‑1 (MQ=255)
                                                                                                                               aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      >  2:1713145/1‑150 (MQ=255)
                                                                                                                               aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGACTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  2:255684/150‑1 (MQ=255)
                                                                                                                               aaaaGAGAACAGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                      <  1:1269479/150‑1 (MQ=255)
                                                                                                                                 aaGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCaa                    >  1:60560/1‑150 (MQ=255)
                                                                                                                                   gagaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCCGCGTTTGCGCGTTTCAACCGCACCGTCGGCGTTCACCTGGCCGTCGGCAATc                  >  2:1301428/1‑150 (MQ=255)
                                                                                                                                   gagaACCGGCCTGTTGAAACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCCCCGTCGACTTTCACCTGGCCTTCGGCAATc                  >  2:858701/1‑150 (MQ=255)
                                                                                                                                    agaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCATTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcg                 >  2:2017309/1‑150 (MQ=255)
                                                                                                                                     gaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcgc                >  1:383223/1‑150 (MQ=255)
                                                                                                                                     gaACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcgc                >  2:249138/1‑150 (MQ=255)
                                                                                                                                      aaCCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCATCAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGAGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACGGTCGACTTTCACCTGGCCTTCGGCAATcgcg               >  1:1291287/1‑150 (MQ=255)
                                                                                                                                        ccGGCCTGTATCAACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt             >  1:538446/1‑150 (MQ=255)
                                                                                                                                        ccGGCCTGTATCAACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt             >  2:1188240/1‑150 (MQ=255)
                                                                                                                                          ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           >  1:1091021/1‑150 (MQ=255)
                                                                                                                                          ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           <  1:1825180/150‑1 (MQ=255)
                                                                                                                                          ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           >  1:15137/1‑150 (MQ=255)
                                                                                                                                          ggCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAttt           >  2:1132865/1‑150 (MQ=255)
                                                                                                                                           gCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGAtttt          >  1:1402250/1‑150 (MQ=255)
                                                                                                                                              tGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGc       >  1:46725/1‑150 (MQ=255)
                                                                                                                                               gTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc      <  2:453814/150‑1 (MQ=255)
                                                                                                                                               gTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc      <  2:1311332/150‑1 (MQ=255)
                                                                                                                                                  gATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc   >  2:78646/1‑150 (MQ=255)
                                                                                                                                                  gATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc   >  1:451300/1‑150 (MQ=255)
                                                                                                                                                   aTACAGGCCGGGTAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAATCTCACTGTCTGACCGGCGACGATTTTGCAGCGGTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgcg  <  2:732220/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
GAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAA‑‑‑GGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCG  >  NC_000913/556495‑556791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: