Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,303,807 T→A 5.2% Q101H (CAA→CAT mdtN ← membrane fusion protein of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,303,8070TA 5.2% 372.8 / 5.1 124Q101H (CAA→CATmdtNmembrane fusion protein of efflux pump
Reads supporting (aligned to +/- strand):  ref base T (62/55);  new base A (3/4);  total (65/59)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

AGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGG  >  NC_000913/4303662‑4303953
                                                                                                                                                 |                                                                                                                                                  
agaCAAAACCTTCTTTCATAAATTGCTAGGTGCGACGTAATGAATCTGGGGCCTGGTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATATGCt                                                                                                                                                <  2:137632/150‑1 (MQ=255)
agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                                                                                <  1:1966096/150‑1 (MQ=255)
agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                                                                                <  2:1436164/150‑1 (MQ=255)
agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                                                                                <  2:1640112/150‑1 (MQ=255)
   cAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTATTCTACCGTGGCATTAACCGAGTCGGAACCAAACTTTTGCGAGTCAACGCTACGCTGGGTGAGCATAATTAGCtaaa                                                                                                                                             >  1:879766/1‑147 (MQ=255)
      aaCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAATCTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCa                                                                                                                                          <  1:162662/150‑1 (MQ=255)
         cttcttTCAGTAATGGCTCGGTGCGTCGTAATGTATCTGTGGCCTGTTTCGCGGAGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAgcg                                                                                                                                       <  1:1152064/150‑1 (MQ=255)
            cttTCAGTAATGGCGCGGTGCGGCGTAATGTATCTGTGGGCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGAACCAAACTGTTGCGCGTCAACGCTACGCTGGATAAGCAAATTATGCTCACCCAgcgtag                                                                                                                                    <  2:1820025/150‑4 (MQ=255)
             tttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGTACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGcgccgc                                                                                                                                   >  2:1686405/1‑150 (MQ=255)
             tttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGcgccgc                                                                                                                                   >  1:1194223/1‑150 (MQ=255)
             tttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGATAAGCAAATTATGCTCACCCAgcgtagc                                                                                                                                   <  2:596809/150‑5 (MQ=255)
                  gTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgaggg                                                                                                                              >  2:951798/1‑150 (MQ=255)
                    aaTGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAATCAGTTGCGAGTCAACGCTACGCTGCGTGAGCATAATTTGCTTATCCATCGCCGCgagggag                                                                                                                            >  2:282457/1‑150 (MQ=255)
                    aaTGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgagggag                                                                                                                            <  1:911203/150‑1 (MQ=255)
                      tGGCTCGGTGCGGAGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGc                                                                                                                          <  1:434388/150‑1 (MQ=255)
                           cGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAg                                                                                                                     <  1:27811/150‑1 (MQ=255)
                           cGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAg                                                                                                                     <  2:1488372/150‑1 (MQ=255)
                              tGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCtt                                                                                                                  >  1:1244798/1‑150 (MQ=255)
                                cggcgTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTc                                                                                                                >  2:660446/1‑150 (MQ=255)
                                   cgTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGGCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGcc                                                                                                             <  2:1640625/150‑1 (MQ=255)
                                     tAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAg                                                                                                           >  2:1828787/1‑150 (MQ=255)
                                      aaTGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGa                                                                                                          <  1:1904568/150‑1 (MQ=255)
                                       aTGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAt                                                                                                         <  1:1594292/150‑1 (MQ=255)
                                         gTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTg                                                                                                       >  1:1723538/1‑150 (MQ=255)
                                         gTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTg                                                                                                       <  2:289429/150‑1 (MQ=255)
                                           aTCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACAGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGc                                                                                                     >  1:592649/1‑150 (MQ=255)
                                            tCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTATCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGGATAATTTGCTTATCCAGCGCCGCGAGGGAGGATTCCGCTTTCGCCAGATTGGcg                                                                                                    >  2:1174217/1‑149 (MQ=255)
                                               gtgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGGCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCg                                                                                                 <  1:549277/150‑1 (MQ=255)
                                               ggggCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCg                                                                                                 <  1:1566440/148‑1 (MQ=255)
                                                          cgcgGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCgg                                                                                      >  1:892303/1‑150 (MQ=255)
                                                            cggcggCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGt                                                                                    >  1:6593/1‑150 (MQ=255)
                                                               cggcACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCATATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCTCGGGTCGa                                                                                 >  2:1903268/1‑150 (MQ=255)
                                                                  cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                              <  1:800349/150‑1 (MQ=255)
                                                                  cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                              >  1:1073806/1‑150 (MQ=255)
                                                                  cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                              <  1:1344652/150‑1 (MQ=255)
                                                                  cACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGATTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                                                                              >  2:1874971/1‑150 (MQ=255)
                                                                   aCGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCg                                                                             <  1:38265/150‑1 (MQ=255)
                                                                   aCGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCg                                                                             <  1:455704/150‑1 (MQ=255)
                                                                       gCTGTTTCTACCGTGGCATTAACCGAGGCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAGTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAAc                                                                         <  1:54942/150‑1 (MQ=255)
                                                                        cttgatCTACCGTGGAATTAACCGAGTCGGCACCAAACTGGTGCGCGGCAACGCGACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACa                                                                        <  1:1272679/145‑1 (MQ=255)
                                                                        cTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACa                                                                        <  2:1314842/150‑1 (MQ=255)
                                                                          ttttCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGc                                                                      <  1:664407/150‑1 (MQ=255)
                                                                              cTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAAt                                                                  >  2:1352241/1‑150 (MQ=255)
                                                                                aCCGTGGCATTAACCGAGTCGGCCCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGGACGGGCGCGGGTCGATGCGGAACAGCAAATCg                                                                >  1:857034/1‑150 (MQ=255)
                                                                                  cGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGcc                                                              >  1:1044026/1‑150 (MQ=255)
                                                                                   gTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCACATCGccc                                                             >  2:934607/1‑150 (MQ=255)
                                                                                   gTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGccc                                                             <  1:943021/150‑1 (MQ=255)
                                                                                     ggCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg                                                           >  1:1133577/1‑150 (MQ=255)
                                                                                     ggCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg                                                           <  1:1398648/150‑1 (MQ=255)
                                                                                     ggCATTAACCGAGTCGGCACCAAACGGTTGCGCGGCAACGCTACGCTGTGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg                                                           <  1:261086/150‑1 (MQ=255)
                                                                                      gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt                                                          >  1:1039141/1‑150 (MQ=255)
                                                                                      gCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt                                                          <  1:2030759/150‑1 (MQ=255)
                                                                                       cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt                                                         >  2:795222/1‑150 (MQ=255)
                                                                                       cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt                                                         >  2:788090/1‑150 (MQ=255)
                                                                                       cATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt                                                         <  2:54737/150‑1 (MQ=255)
                                                                                        aTTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGttt                                                        >  2:189716/1‑150 (MQ=255)
                                                                                         ttAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACTGCAAATCGCCCTGTTTg                                                       >  1:2043569/1‑150 (MQ=255)
                                                                                           aaCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGAc                                                     >  2:558764/1‑150 (MQ=255)
                                                                                           aaCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGAc                                                     <  2:1596451/150‑1 (MQ=255)
                                                                                           aaCCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAATTGCTTATCCAGCGCCGCGAGGGAGGCATCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGACCAGCAAATCGCCCTGTTTGAc                                                     >  1:1822905/1‑150 (MQ=255)
                                                                                             ccGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTg                                                   <  2:1781786/150‑1 (MQ=255)
                                                                                              cGAGTCGGCTCCAAACTGTTGCGCGTCAACGCTACGCTGGGTTAGCATAATTTGATTATCCAGCGCCGCGAGTGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGc                                                  <  2:359537/150‑1 (MQ=255)
                                                                                                 gTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTAGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTg                                               >  2:499005/1‑150 (MQ=255)
                                                                                                 gTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGAGCGCAAAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCATTCGCCAGATTGGCTTCGTACGGGCGCGTCTCGATGCGGAACAGCAACTCGACCTGTTTGACTGCCTg                                               >  1:1140463/1‑150 (MQ=255)
                                                                                                     gCACCAAACTGTTGCGCTTCAACGCTACGCTTGGTGAGTATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTg                                           <  2:2013311/150‑1 (MQ=255)
                                                                                                      cACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGt                                          >  1:1568763/1‑150 (MQ=255)
                                                                                                       aCCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTc                                         <  2:71402/150‑1 (MQ=255)
                                                                                                        ccAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCg                                        >  2:1009683/1‑150 (MQ=255)
                                                                                                         cAACCTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCTGCTTTCGCCAGATTGGCTTCGTACGGGAGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCgg                                       <  2:1789008/150‑1 (MQ=255)
                                                                                                         cAAACTGTTGCGCGGCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCgg                                       <  1:1482690/150‑1 (MQ=255)
                                                                                                               gTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCg                                 <  1:1828788/150‑1 (MQ=255)
                                                                                                                 tGCTCGTCAACGCTACGCTGTGTGAGCATAATTTGCTTATCCAGCGCATCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGTTCGTTGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCTGTGACCGcc                               <  2:879765/150‑1 (MQ=255)
                                                                                                                 tGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGcc                               <  2:719553/150‑1 (MQ=255)
                                                                                                                 tGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGcc                               >  1:359822/1‑150 (MQ=255)
                                                                                                                   cgcgTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAg                             >  1:658402/1‑150 (MQ=255)
                                                                                                                   cgccTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAg                             <  2:1468523/150‑1 (MQ=255)
                                                                                                                      gTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTc                          >  1:1649453/1‑150 (MQ=255)
                                                                                                                        cAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTa                        >  1:291291/1‑150 (MQ=255)
                                                                                                                          acgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACa                      >  1:902137/1‑150 (MQ=255)
                                                                                                                           cgctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACaa                     >  1:1151808/1‑150 (MQ=255)
                                                                                                                           cgcggcgctGGATAAGCAAATTATGCTCACCCAGCGTAGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACaa                     >  2:1154811/6‑150 (MQ=37)
                                                                                                                            gcggcgctGGATAAGCAAATTATGCTCACCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAAt                    >  1:669304/5‑150 (MQ=255)
                                                                                                                             ctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATg                   <  1:1686406/150‑1 (MQ=255)
                                                                                                                             ctacgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATg                   >  2:1413810/1‑150 (MQ=255)
                                                                                                                               acgctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGCCCGCCAGTTCTACAATGCg                 >  1:1764649/1‑150 (MQ=255)
                                                                                                                                 gctGGGTGAGCATAATTTTCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGc               <  1:1027311/150‑1 (MQ=255)
                                                                                                                                  ctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGcc              >  1:1930510/1‑150 (MQ=255)
                                                                                                                                  ctGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCACTTCTACAATGCGGcc              >  2:1309021/1‑150 (MQ=255)
                                                                                                                                     ggTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCt           >  1:1569653/1‑150 (MQ=255)
                                                                                                                                      gTGAGCATACTTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTg          <  2:841704/150‑1 (MQ=255)
                                                                                                                                      gTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTg          <  1:1089202/150‑1 (MQ=255)
                                                                                                                                      gTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTGCTACAATGCGGCCGCTg          <  1:1352242/150‑1 (MQ=255)
                                                                                                                                       tGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGa         <  1:547181/150‑1 (MQ=255)
                                                                                                                                        gAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGAc        >  2:1807154/1‑150 (MQ=255)
                                                                                                                                         aGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGt                                          <  1:1220221/115‑1 (MQ=255)
                                                                                                                                         aGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGt                                          >  2:1220220/1‑115 (MQ=255)
                                                                                                                                          gCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACtt      >  1:1976944/1‑150 (MQ=255)
                                                                                                                                             tAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGCCTTCCg   >  2:1070649/1‑150 (MQ=255)
                                                                                                                                             tAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCg   >  1:867723/1‑150 (MQ=255)
                                                                                                                                              aaTTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTAAAATGCGGCCACTGACTTCCgg  >  1:281719/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGACTGCCTGGTTGTCGGTGACCGCCAGTTCTACAATGCGGCCGCTGACTTCCGG  >  NC_000913/4303662‑4303953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: