Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,307,339 C→A 6.3% W154L (TGG→TTG)  alsK ← D‑allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,3390CA 6.3% 245.8 / 2.4 90W154L (TGG→TTG) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base C (44/39);  new base A (2/5);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTA  >  NC_000913/4307190‑4307488
                                                                                                                                                     |                                                                                                                                                     
aaTTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTcc                                                                                                                                                       <  2:1247249/150‑1 (MQ=255)
aaTTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTcc                                                                                                                                                       >  1:2030969/1‑150 (MQ=255)
  attCGCGGCTGCGGGTCGTACCAGCGTCTTACCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGGTCGCCTGCCACACCGTGTGCACCCGTCCAc                                                                                                                                                     <  2:75430/149‑1 (MQ=255)
    tCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACgg                                                                                                                                                   <  1:674555/150‑1 (MQ=255)
       gggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGgcgc                                                                                                                                                <  2:1147444/150‑1 (MQ=255)
          cTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTGTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                                                                                                                             <  2:893717/150‑1 (MQ=255)
           tgttgtTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCg                                                                                                                                            >  1:1716074/1‑150 (MQ=255)
            gttgttCGTACCAGCGTCTTAGCGCCATTGCAGAGCAATAGATTTCCAGGCACACAGGATTGCCACACGCGCAGAGTTGGGTCATATCTCACAGGGGGATATGACCCAGTTCGACTGCCACACCGTGTGCCCCCGTCCACGTCGCACCGt                                                                                                                                           >  1:139349/1‑150 (MQ=255)
            gttgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCACAGGGGGATATGACCCAGTTAGCCTGCCACACCGTGTGCACCCGTCCCCGGCGCCCCGt                                                                                                                                           >  1:664676/1‑150 (MQ=255)
            gttgttCATACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATATCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCCAGGCGCACTGt                                                                                                                                           >  1:1966138/1‑150 (MQ=255)
              tgttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTc                                                                                                                                         <  1:1921074/150‑1 (MQ=255)
               gttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCCCCCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACCCCGTGTGCACCCGTCCACGGCGCCCCGTTCa                                                                                                                                        >  1:1598104/1‑150 (MQ=255)
               gttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa                                                                                                                                        >  2:965625/1‑150 (MQ=255)
               gttCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCa                                                                                                                                        >  1:1108867/1‑150 (MQ=255)
                 tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCAAGTCACCCGGGATAGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGAGCAACCGTCAACGGCGCACCGTTCATc                                                                                                                                      >  1:1429122/1‑150 (MQ=255)
                  cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATcc                                                                                                                                     <  1:1420707/150‑1 (MQ=255)
                  cGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGATCATcc                                                                                                                                     >  2:516712/1‑150 (MQ=255)
                    tACCAGCGTGTTAGCGACATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTGATTTCTACCAGGGGTATATGACCCAGTTCGCCTGCCACACCGTGTGAACCCGTCCACGGCGCACCGTTCATCcac                                                                                                                                   <  2:1584949/150‑1 (MQ=255)
                    tACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCAACGGCGCACCGTTCATCcac                                                                                                                                   >  2:1419480/1‑150 (MQ=255)
                      ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCTACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCacac                                                                                                                                 >  1:748470/1‑150 (MQ=255)
                      ccAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCacac                                                                                                                                 >  2:1903890/1‑150 (MQ=255)
                         gCGTCTTAGCGCCATTCCTGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCTCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCAGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGc                                                                                                                              <  1:708104/150‑1 (MQ=255)
                           gTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGa                                                                                                                            <  1:1638944/150‑1 (MQ=255)
                               tAGCGATATTCCAGAGCAATTCGTTTCAAGGCACACAGTATTGCCACACGCGCAGTGGTGGGGAAGATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCTCACCGTGTTCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAAccc                                                                                                                        <  1:1240211/150‑1 (MQ=255)
                                  cgcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGTGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAt                                                                                                                     <  1:1402476/150‑1 (MQ=255)
                                   gcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCCTCCACGGCGCACCGTTCCTCCACACTGCGAACCCCagc                                                                                                                    >  2:1066978/1‑148 (MQ=255)
                                   gcCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCCTGTGCACCCCTCCACGGCGCACCGTTAATCCACACAGCCAACCCCaaa                                                                                                                    >  2:214253/1‑148 (MQ=255)
                                     cATTCCAGAGCAATTGGGTTCCAGGCACCCAGGCTTGCCACACGCGCAGTGGTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGAGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATccc                                                                                                                  <  1:1402371/150‑1 (MQ=255)
                                        tCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGt                                                                                                               <  1:674171/150‑1 (MQ=255)
                                         ccAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTa                                                                                                              <  1:126699/150‑1 (MQ=255)
                                          cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACCCCGTGTGCACCCGGCCACGGCGCCCCGTGCATCCACACTGCGAACACCATCCCCGtcc                                                                                                             >  1:1097542/1‑148 (MQ=255)
                                          cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCACAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTGCATCCACACTGCGCACCCCCACCCCGtcc                                                                                                             >  1:1090524/1‑148 (MQ=255)
                                          cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc                                                                                                             >  2:702365/1‑150 (MQ=255)
                                          cAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAc                                                                                                             >  2:1789787/1‑150 (MQ=255)
                                           agagCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCATTTTTTTTTTTAATGTTAAGGGGTCAACGTTGATCTACACTGCGAACCCCATCCCCGTAcc                                                                                                            <  2:37146/150‑1 (MQ=255)
                                              gCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCgag                                                                                                         >  1:588848/1‑150 (MQ=255)
                                                aaTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACCCTGCGAACCCCATCCCCGTACCGAGAt                                                                                                       >  2:746730/1‑150 (MQ=255)
                                                aaTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGCACCCCAGCCCCGGACCGAGAt                                                                                                       >  1:497352/1‑150 (MQ=255)
                                                 aTTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATa                                                                                                      <  2:1716073/150‑1 (MQ=255)
                                                    ggTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCCCCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgc                                                                                                   >  2:156413/1‑150 (MQ=255)
                                                        tCCAGGCACCCAGGATTGTCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTGCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGgccgcc                                                                                               <  1:2036665/150‑1 (MQ=255)
                                                         ccAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGAAACCGTCCACGGCGCACCGTTGATCCACACTGCGCGCCCCATCACCGTACCGAGTAAGgccgcgg                                                                                              >  1:379978/1‑148 (MQ=255)
                                                          cAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAg                                                                                             >  2:443141/1‑150 (MQ=255)
                                                             gCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAAc                                                                                          <  2:1662550/150‑1 (MQ=255)
                                                                 ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCTCCTGCCACAAAGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgt                                                                                      <  2:117163/150‑1 (MQ=255)
                                                                 ccAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgt                                                                                      <  2:2030968/150‑1 (MQ=255)
                                                                  cAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgtt                                                                                     >  1:1204498/1‑150 (MQ=255)
                                                                   aGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgttg                                                                                    <  1:960012/150‑1 (MQ=255)
                                                                     gATAGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCAAATATTTTTTAAATGTTAAGGGGGCAACGTTGATCTACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAgttgtt                                                                                  <  2:262043/150‑1 (MQ=255)
                                                                      aTTGCCACCCGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTGCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGttgttg                                                                                 <  2:1131635/150‑1 (MQ=255)
                                                                      aTTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAACCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGttgttg                                                                                 <  2:66213/150‑1 (MQ=255)
                                                                       tggCCACACGCGCAGTGTTGGGTCATATCTCCCAGTGGGATATGACCCAGTTCGCCTGCCATTTTTTTTTTTAATGTTAAGGGGTCAACGGTGATCTACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGc                                                                                <  2:29991/148‑1 (MQ=255)
                                                                                gcgcTGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACTTTTTTTTTTAATGATAAGGGGTCAACGGTGATCTACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGctg                                                                       <  2:38683/150‑3 (MQ=255)
                                                                                 cgcAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGt                                                                      <  2:705586/150‑1 (MQ=255)
                                                                                   cAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATTGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGttt                                                                    >  2:1741252/1‑150 (MQ=255)
                                                                                   cAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGttt                                                                    <  1:1521477/150‑1 (MQ=255)
                                                                                     gtgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACTCCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc                                                                  <  2:1987874/150‑1 (MQ=255)
                                                                                     gtgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTc                                                                  <  1:1031928/150‑1 (MQ=255)
                                                                                      tgtTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTct                                                                 <  1:1532071/150‑1 (MQ=255)
                                                                                      tgtTGGGGCATATCTCCCAGGGGTATATGACCCAGTTCGCCTGCCATTTTTTTTTTTAATGTTAAGGGGTCAACGGTGATCTACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTct                                                                 <  2:1395223/150‑1 (MQ=255)
                                                                                        ttGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctac                                                               <  1:1903891/150‑1 (MQ=255)
                                                                                          ggggaatatCTCCCAGGGGGATATGACCCAGTTCGCCTGCAACACCGTTTGCACCCGTCCACGGCGCACCGTTGATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTctacta                                                             <  2:584879/145‑1 (MQ=255)
                                                                                          gggTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCCCCCGTCCACGGCGCACCGTTCATCGACACTGCGAACCCCATGCCCGTACCGAGATAGGCCGCCAGCACCAGGTGTGGCGGAAGGCGGTTTTctgctt                                                             >  1:1058134/1‑149 (MQ=255)
                                                                                           ggTCATATCCCCCTGGGAGATATGACCCAGTTCGCCTGCCACACCGTGTGCAACCGTCCACGGCGCACCGTTCATCCACACGGAGAACGACATCCCCGAACCGAGATAGGCCGCCAGCACCAGGTGTGGCGTAAGGCGGTTTTCtactac                                                            >  2:52485/1‑150 (MQ=255)
                                                                                               atatCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTcc                                                        >  2:1719159/1‑150 (MQ=255)
                                                                                                 atCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTCAGGCGGTTTTCTACTACGTCCCa                                                      >  2:2021868/1‑150 (MQ=255)
                                                                                                   ctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAgg                                                    >  1:1148120/1‑150 (MQ=255)
                                                                                                   ctcCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAgg                                                    >  2:1163797/1‑150 (MQ=255)
                                                                                                       cAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCAACCCCGTACCGAGATAGGCCGCCAGCCCCAGTTGTTGCGGAAGGAGGTGTTCTACTACGTCCCAGGagag                                                >  2:1320210/1‑150 (MQ=255)
                                                                                                       cAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAACGTAAAACCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGagag                                                >  1:1008563/1‑150 (MQ=255)
                                                                                                          ggggATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTg                                             >  2:1645422/1‑150 (MQ=255)
                                                                                                             gATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAg                                          <  1:1574476/150‑1 (MQ=255)
                                                                                                                atGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGtt                                       >  2:848492/1‑150 (MQ=255)
                                                                                                                  gACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTaa                                     <  1:1909890/150‑1 (MQ=255)
                                                                                                                  gACCCAGTTCGCCTGCCACACCGTGTGCACACGTCCACGGCGCACCGTTCATCCACACAGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGACCCAGTTGTTGCGTAAGGCGGTTATCTACTAGGTCCCAGGAGAGGTGCAGGTTag                                     >  1:874307/1‑149 (MQ=255)
                                                                                                                    cccAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACg                                   <  2:1108866/150‑1 (MQ=255)
                                                                                                                       aGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTcg                                >  2:29078/1‑150 (MQ=255)
                                                                                                                         ttCGCCTGCCTCACCGTGTGCACCCGTCCACGGGGCTCCGTTCATCTACACTGCGAAGCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTcgcg                              <  2:1677336/150‑1 (MQ=255)
                                                                                                                           cGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACCCTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCggg                            <  1:1508268/150‑1 (MQ=255)
                                                                                                                           cGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCTGAACCAGTTCTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCggg                            >  2:1223482/1‑150 (MQ=255)
                                                                                                                            gCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGa                           >  1:1577655/1‑150 (MQ=255)
                                                                                                                            gCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTGCTACTCCGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGa                           >  1:1149958/1‑150 (MQ=255)
                                                                                                                                gCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAAc                       <  1:1069620/150‑1 (MQ=255)
                                                                                                                                 ccACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGCACCCCCTCCCCGTCCCGAGATAGGCCGCCAGACCCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACt                      >  1:1319045/1‑150 (MQ=255)
                                                                                                                                   acacCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCa                    <  1:443141/150‑1 (MQ=255)
                                                                                                                                      ccGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCCAcc                 >  2:1981259/1‑150 (MQ=255)
                                                                                                                                       cGTGTGCACCCGTCCACGGCGCACTGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCg                <  2:1966137/150‑1 (MQ=255)
                                                                                                                                          gtgCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTCCCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCGAGGAGAGTTGCAGGTTAACGTCGCGGGATAACTCATCCGGAc             >  1:137721/1‑150 (MQ=255)
                                                                                                                                          gtgCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGAc             <  2:588848/150‑1 (MQ=255)
                                                                                                                                           tgCACCCGGCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTGGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACa            <  2:1921154/150‑1 (MQ=255)
                                                                                                                                               cccGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTAGCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAACACTCTACCGGACATTTc        >  1:1286543/1‑150 (MQ=255)
                                                                                                                                               cccGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTATGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGATAACGTCGCGGGAAAACTCAACCGGACAATTc        >  2:361697/1‑150 (MQ=255)
                                                                                                                                                     cACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTa  >  2:505879/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACACTGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTA  >  NC_000913/4307190‑4307488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑