Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,309,875 T→G 5.1% K369T (AAA→ACA)  alsA ← D‑allose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,309,8750TG 5.1% 391.5 / 7.9 130K369T (AAA→ACA) alsAD‑allose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (52/71);  new base G (2/5);  total (54/76)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

Only 100 of 134 total aligned reads displayed.

GGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATT  >  NC_000913/4309726‑4310024
                                                                                                                                                     |                                                                                                                                                     
ggACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCtt                                                                                                                                                       >  2:1965239/1‑150 (MQ=255)
    tttCTGCTGCTTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAg                                                                                                                                                   <  1:1825878/150‑1 (MQ=255)
          ctgATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCg                                                                                                                                             <  2:6885/150‑1 (MQ=255)
            gATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGc                                                                                                                                           <  1:212086/150‑1 (MQ=255)
            gATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGc                                                                                                                                           <  2:182410/150‑1 (MQ=255)
             aTTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCt                                                                                                                                          <  2:1960853/150‑1 (MQ=255)
             aTTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCAGTCTGAAAGACGGCGGCt                                                                                                                                          <  1:1115457/150‑1 (MQ=255)
              tttCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCTGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTg                                                                                                                                         <  2:1534738/150‑1 (MQ=255)
               ttCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGa                                                                                                                                        >  1:1444261/1‑150 (MQ=255)
                tCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGAt                                                                                                                                       <  1:1521521/150‑1 (MQ=255)
                tCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCAGTCTGAAAGACGGCGGCtata                                                                                                                                       <  2:1389416/150‑4 (MQ=255)
                tCCCCCGGAGAGAGCGGGGATATTCTGGATTCCCGAATGACATTTCAGCGCCAGCAGTTCCCGGTGATTTTCAGCGCAACGTTGCTCGGCAACTTCATGCACCAAGCCCATCCCGCCTTTATCGCCGCCGGCTTTCAGACTGCGGCTGAt                                                                                                                                       <  2:97412/150‑1 (MQ=255)
                  ccccGGAGAGTTCGGTGATTTTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCg                                                                                                                                     <  2:836003/150‑1 (MQ=255)
                   cccGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGc                                                                                                                                              <  2:1012702/140‑1 (MQ=255)
                   cccGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGc                                                                                                                                              >  1:1012703/1‑140 (MQ=255)
                    ccGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCAGTCTGAAAGACGGCGGCtataaagg                                                                                                                                   <  2:926108/150‑8 (MQ=255)
                    ccGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGAGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGcc                                                                                                                                   <  2:1382254/150‑1 (MQ=255)
                     cGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCa                                                                                                                                  <  2:473539/150‑1 (MQ=255)
                     cGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCGCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCa                                                                                                                                  <  1:1016513/150‑1 (MQ=255)
                      ggAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCAt                                                                                                                                 >  2:962061/1‑150 (MQ=255)
                         gagTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCTGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCAATgat                                                                                                                              >  1:149684/1‑148 (MQ=255)
                           gTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCt                                                                                                                            <  1:1575033/150‑1 (MQ=255)
                            ttCGGTGATATTCTGGTTTACCGAATGTCATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTAAGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTg                                                                                                                           <  2:929002/150‑1 (MQ=255)
                            ttCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTg                                                                                                                           <  2:1704684/150‑1 (MQ=255)
                             tCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCGCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGa                                                                                                                          <  1:1328289/150‑1 (MQ=255)
                                    tatTCTGGTTTACCGAATGACATTTCAGCGCCAGGAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAAGTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATgg                                                                                                                   <  1:296447/150‑1 (MQ=255)
                                    tatTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATgg                                                                                                                   <  1:1140214/150‑1 (MQ=255)
                                    tatTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATgg                                                                                                                   >  2:1113889/1‑150 (MQ=255)
                                       tCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGaaa                                                                                                                <  1:1389905/150‑1 (MQ=255)
                                              tACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTggg                                                                                                         <  2:1352722/150‑1 (MQ=255)
                                              tACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTggg                                                                                                         <  2:837886/150‑1 (MQ=255)
                                               aCCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTgggg                                                                                                        >  2:336288/1‑150 (MQ=255)
                                                 cGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGaa                                                                                                      >  1:617245/1‑150 (MQ=255)
                                                 cGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGaa                                                                                                      >  1:857479/1‑150 (MQ=255)
                                                     agACATTTCAGCGCCAGCAGTTCGCGTTGAGTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCTGACTGCGGCTGATCGCCATGTTCGGAGCGATGGAAAAGTTGGGGaaaacc                                                                                                  >  1:538062/2‑148 (MQ=255)
                                                      gACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAAc                                                                                                 >  1:1752369/1‑150 (MQ=255)
                                                        cATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCg                                                                                               <  1:909206/150‑1 (MQ=255)
                                                         aTTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGt                                                                                              <  1:1216405/150‑1 (MQ=255)
                                                         aTTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGt                                                                                              <  1:532210/150‑1 (MQ=255)
                                                           ttCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTa                                                                                            >  1:730586/1‑150 (MQ=255)
                                                              aGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATcc                                                                                         <  1:618506/150‑1 (MQ=255)
                                                              aGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATcc                                                                                         >  1:2024719/1‑150 (MQ=255)
                                                               gcgcCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATccc                                                                                        >  1:1640344/1‑150 (MQ=255)
                                                                  ccAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCcggc                                                                                     <  1:164574/150‑1 (MQ=255)
                                                                  ccAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCcggc                                                                                     <  2:1860296/150‑1 (MQ=255)
                                                                     gcagTTCGCGTTGATTTTCAGCGGTACGTTGCTCGGCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCggcggc                                                                                  <  2:1330134/150‑1 (MQ=255)
                                                                        gTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCttt                                                                               <  2:623148/150‑1 (MQ=255)
                                                                        gTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCttt                                                                               >  2:1134666/1‑150 (MQ=255)
                                                                           cgcgTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAg                                                                            <  2:60506/150‑1 (MQ=255)
                                                                            gcgTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGt                                                                           <  1:490157/150‑1 (MQ=255)
                                                                             cgTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCAATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGtt                                                                          >  1:1723233/1‑149 (MQ=255)
                                                                              gTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTCTAGCAGCCGTCTTTCAGACTGAGTCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAGCCGTGTTCCCGGCGGCTTTCAGtca                                                                         >  1:297526/1‑148 (MQ=255)
                                                                                tGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTTCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATg                                                                       <  2:307710/150‑1 (MQ=255)
                                                                                tGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGGCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATg                                                                       <  2:1242013/150‑1 (MQ=255)
                                                                                       cAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATACCGGCGGCTTTCAGTGATGTAAGCCa                                                                >  1:655758/1‑150 (MQ=255)
                                                                                         gCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATc                                                              >  2:1426345/1‑150 (MQ=255)
                                                                                            gTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCt                                                           <  2:715614/150‑1 (MQ=255)
                                                                                             tACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCtt                                                          >  2:305824/1‑150 (MQ=255)
                                                                                               cGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCAGGTTCTGAGCGAGGGAAAAGTTGGGGAAAACACCGTTATCCCGGCGGCTTTCAGTGATGTCAGCCATgccggtt                                                        >  2:215349/1‑146 (MQ=255)
                                                                                                 ttGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCtttttt                                                      >  2:983844/1‑150 (MQ=255)
                                                                                                 ttGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCtttttt                                                      >  2:889950/1‑150 (MQ=255)
                                                                                                 ttGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCtttttt                                                      <  1:40428/150‑1 (MQ=255)
                                                                                                  tGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTc                                                     >  1:1339627/1‑150 (MQ=255)
                                                                                                  tGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTATTc                                                     >  1:1943879/1‑150 (MQ=255)
                                                                                                   gCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCa                                                    <  1:1918696/150‑1 (MQ=255)
                                                                                                     tCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGTAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACg                                                  <  1:746521/150‑1 (MQ=255)
                                                                                                     tCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACg                                                  <  1:1487478/150‑1 (MQ=255)
                                                                                                      cGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACgg                                                 <  2:1444260/150‑1 (MQ=255)
                                                                                                      cGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACgg                                                 <  2:1584072/150‑1 (MQ=255)
                                                                                                      cGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACgg                                                 <  2:1928652/150‑1 (MQ=255)
                                                                                                              tCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAgg                                         >  2:636075/1‑150 (MQ=255)
                                                                                                               cATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAACCCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAggg                                        >  2:377889/1‑150 (MQ=255)
                                                                                                                aTGAACCAAGCCCATCGCGCCTTTATAGCCGCCGTCTGTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAgggg                                       <  2:88557/150‑1 (MQ=255)
                                                                                                                aTGAAACAAGCCCCTCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAgggg                                       <  2:146321/150‑1 (MQ=255)
                                                                                                                 tGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCGCTTTTTTCCCGGCTTCCAGGGGt                                      >  1:1284906/1‑150 (MQ=255)
                                                                                                                 tGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGt                                      >  2:1498235/1‑150 (MQ=255)
                                                                                                                  gAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTg                                     >  1:2013290/1‑150 (MQ=255)
                                                                                                                    aaCAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTGTTTTCACGGCATCCAGGGGTGaa                                   >  2:1518898/1‑150 (MQ=255)
                                                                                                                       aaGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTTTCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGt                                >  2:1015545/1‑150 (MQ=255)
                                                                                                                       aaGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTTTCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTAACGGCTTCCAGGGGTGAACGt                                >  1:434214/1‑150 (MQ=255)
                                                                                                                       aaGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGt                                <  2:1497376/150‑1 (MQ=255)
                                                                                                                        aGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCCCGGCATCCAGGGGTGAACGTg                               >  2:108132/1‑150 (MQ=255)
                                                                                                                        aGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTg                               <  2:128251/150‑1 (MQ=255)
                                                                                                                        aGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCGTTCAGTGATGTAAGCCCTCCCTTTTTTCACGGCATCCAGGGGTGAACGTg                               >  1:1927212/1‑150 (MQ=255)
                                                                                                                         gCCCATCGCGCCTTTATATCCGCAGTCTTTCAGACTGCGGCTGATCGCCATGATCTTCGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTgg                              <  1:226808/150‑1 (MQ=255)
                                                                                                                           ccATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGgag                            <  1:1050067/150‑1 (MQ=255)
                                                                                                                            cATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTAGCACGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCGGGGGTGAACTGGgaga                           >  1:425101/1‑150 (MQ=255)
                                                                                                                             aTCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGgagag                          <  1:638971/150‑1 (MQ=255)
                                                                                                                                gcgcCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGata                       <  2:1723232/150‑1 (MQ=255)
                                                                                                                                    cTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCTTGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGGGGAGAGATATCtt                   <  2:1583063/150‑1 (MQ=255)
                                                                                                                                    cTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCtt                   <  2:765764/150‑1 (MQ=255)
                                                                                                                                    cTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCtt                   >  1:1763239/1‑150 (MQ=255)
                                                                                                                                     cgatcaGCCGCAGTCTGAAAGACGGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTGGGGGAAAACACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCttt                  >  1:1227691/6‑150 (MQ=255)
                                                                                                                                       tataGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGc                <  1:852042/150‑1 (MQ=255)
                                                                                                                                           gccgcaGTCTGAAAGACGGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCAtt            >  2:657766/1‑150 (MQ=255)
                                                                                                                                           cccgccgTCTTTCTCACTGCGGTTGATCCCAATGTTCTTACCTATGTAAAATTTGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTa           <  1:1171835/149‑1 (MQ=255)
                                                                                                                                              gccgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAg         <  1:1586452/150‑1 (MQ=255)
                                                                                                                                                 gTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACg      <  2:660910/150‑1 (MQ=255)
                                                                                                                                                     ttCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAtt  >  2:199375/1‑150 (MQ=255)
                                                                                                                                                     ttCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGAtt  >  2:1744508/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGACTTTCTGCTGATTTCCCCCGGAGAGTTCGGTGATATTCTGGTTTACCGAATGACATTTCAGCGCCAGCAGTTCGCGTTGATTTTCAGCGGTACGTTGCTCGTCAACTTCATGAAACAAGCCCATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTTGCCATTAAGACGGATT  >  NC_000913/4309726‑4310024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: