Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,390,403 A→G 5.5% intergenic (‑43/+54) psd ← / ← rsgA phosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,390,4030AG 5.5% 441.3 / 10.1 145intergenic (‑43/+54)psd/rsgAphosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base A (72/64);  new base G (4/4);  total (77/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 145 total aligned reads displayed.

TTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGC  >  NC_000913/4390256‑4390546
                                                                                                                                                   |                                                                                                                                               
    gTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGcc                                                                                                                                           <  1:47265/150‑1 (MQ=255)
    gTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGcc                                                                                                                                           >  1:1380772/1‑150 (MQ=255)
      cAGCCATCCTGCCCGCTTTCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAATGTTTAAATGAATTTAACAAGGTAGACTACAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                         >  1:816935/1‑150 (MQ=255)
       aGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACTTGCgggg                                                                                                                                        >  1:371148/1‑149 (MQ=255)
       aGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGa                                                                                                                                        <  1:248773/150‑1 (MQ=255)
        gCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGAt                                                                                                                                       >  2:1649143/1‑150 (MQ=255)
        gCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGAt                                                                                                                                       >  2:1199488/1‑150 (MQ=255)
         ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATc                                                                                                                                      <  1:487794/150‑1 (MQ=255)
         ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTGCGGCAGAATGTACGGTAGCGAAAGTGTAAATGATTTTTAGAAGGTAGGCTGCAGGCCTTGGTTTGGTCGATCCTGCTCCGGGCTGCTTGCCGGAtg                                                                                                                                      >  2:327109/1‑149 (MQ=255)
           ctCCTGACCGCTAGCATGCTCCCCACCCCGCCAGGCGAGTAATCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTACCAATGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGATcag                                                                                                                                    <  1:1400319/149‑3 (MQ=255)
           aTCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGATcag                                                                                                                                    <  1:1238923/150‑3 (MQ=255)
            tCCTGCCCGCTTGCTTGCGCCCCAACCCGTCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                   >  1:2046321/1‑150 (MQ=255)
            tCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                   <  2:34015/150‑1 (MQ=255)
            tCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                   <  1:590731/150‑1 (MQ=255)
             ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCTTTCCTGATCAGGCCTACAAGCCGGAGCCTGa                                                                                                                                  >  2:569780/1‑150 (MQ=255)
             ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGa                                                                                                                                  >  2:374216/1‑150 (MQ=255)
              cTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaa                                                                                                                                 >  1:1705416/1‑150 (MQ=255)
               tGCCCGCTTGCTTGCGCCCCACCCCGCCAGGCGAGGAAGCCATAGTTTCGGCAGACTCTACGGTAGCGAAAGTATAAATGAATTTGACAAGGTAGCCTCCAGGCGCGCGTGTTGTCGTTCCTGATCCGCACTCAAAGCCGGAGCCTGaaa                                                                                                                                >  1:88726/1‑150 (MQ=255)
               tGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaa                                                                                                                                <  2:696856/150‑1 (MQ=255)
               tGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaa                                                                                                                                >  2:1652094/1‑150 (MQ=255)
                gCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaa                                                                                                                               <  1:98868/150‑1 (MQ=255)
                  ccGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaaaa                                                                                                                             >  2:11565/1‑150 (MQ=255)
                  ccGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaaaa                                                                                                                             >  1:2020779/1‑150 (MQ=255)
                   cGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGTCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaaaaa                                                                                                                            <  1:309316/150‑1 (MQ=255)
                    gcttgcttGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAg                                                                                                                           <  1:709314/150‑1 (MQ=255)
                     cttgcttgCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAgg                                                                                                                          >  2:28146/1‑150 (MQ=255)
                      ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                         <  1:792185/150‑1 (MQ=255)
                      ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                         <  2:1365702/150‑1 (MQ=255)
                        gcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGa                                                                                                                       >  1:260333/1‑150 (MQ=255)
                           tgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGa                                                                                                                    <  1:216807/150‑1 (MQ=255)
                              gcCCCACCCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTc                                                                                                                 >  2:401742/1‑150 (MQ=255)
                              gcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTc                                                                                                                 <  1:183520/150‑1 (MQ=255)
                              gcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTc                                                                                                                 <  1:1959128/150‑1 (MQ=255)
                               ccccAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCt                                                                                                                <  2:47772/150‑1 (MQ=255)
                                  cAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAac                                                                                                             <  2:1718596/150‑1 (MQ=255)
                                  cAACCCCCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAac                                                                                                             <  1:1940374/150‑1 (MQ=255)
                                     cccTCCAGGCTAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGTTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgac                                                                                                          <  1:1731784/150‑1 (MQ=255)
                                     cccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACCTGCCGGATCCTGAAAAAAAGGGGACGAGTCTAacgac                                                                                                          >  1:1047802/1‑150 (MQ=255)
                                      ccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgacg                                                                                                         >  2:738236/1‑150 (MQ=255)
                                           aGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAg                                                                                                    <  1:1836445/150‑1 (MQ=255)
                                           aGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAg                                                                                                    <  2:1662352/150‑1 (MQ=255)
                                             gCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCt                                                                                                  <  1:1154038/150‑1 (MQ=255)
                                              cGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCtt                                                                                                 <  2:1464059/150‑1 (MQ=255)
                                                    aGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgt                                                                                           >  2:44996/1‑150 (MQ=255)
                                                     gCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAATAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtc                                                                                          >  2:183442/1‑150 (MQ=255)
                                                        aTAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcagt                                                                                       <  1:1672881/150‑1 (MQ=255)
                                                        aTAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcagt                                                                                       >  1:645728/1‑150 (MQ=255)
                                                         tAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcagtc                                                                                      >  1:1418203/1‑150 (MQ=255)
                                                             ttCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATcc                                                                                  >  2:1892984/1‑150 (MQ=255)
                                                               cGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGt                                                                                <  2:1726876/150‑1 (MQ=255)
                                                                ggCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTa                                                                               >  1:1294692/1‑150 (MQ=255)
                                                                 gCAGAATGTACTGTAGCGAAAGTTTTTATGAATTTAACAAGTTAGCCTACAGTCCATTGTTTTTTCGTTCCTGATCCTGCCTAAATGCAGGATCCTGAAAAAAAGGGGAAGATTATAACCACGATTAGATTAATTGTCTGTCATCCGTAt                                                                              >  1:104870/1‑150 (MQ=255)
                                                                  cAGAATGTACTGTAGCGAAAGATTAAATGAATTTAAAAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATc                                                                             <  1:36465/150‑1 (MQ=255)
                                                                    gAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAg                                                                           <  1:689378/150‑1 (MQ=255)
                                                                      aTGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaa                                                                         >  2:698232/1‑150 (MQ=255)
                                                                      aTGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTAAGTCATCCGTATCAGaa                                                                         >  2:761358/1‑150 (MQ=255)
                                                                       tGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaaa                                                                        >  1:661931/1‑150 (MQ=255)
                                                                        gTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaaaa                                                                       >  1:188277/1‑150 (MQ=255)
                                                                              tAGCTAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGttttt                                                                 <  1:531648/150‑1 (MQ=255)
                                                                              tAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGttttt                                                                 >  2:454407/1‑150 (MQ=255)
                                                                                         tAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCAGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTa                                                      >  1:107876/1‑150 (MQ=255)
                                                                                           aaTGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCCTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTAcc                                                    <  2:2049483/150‑1 (MQ=255)
                                                                                             tGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTg                                                  <  2:849415/150‑1 (MQ=255)
                                                                                               aaTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcg                                                <  1:1718836/150‑1 (MQ=255)
                                                                                                aTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcgc                                               >  1:777153/1‑150 (MQ=255)
                                                                                                 tttAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGcc                                              >  2:1126102/1‑150 (MQ=255)
                                                                                                     aCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGc                                          >  2:2049975/1‑150 (MQ=255)
                                                                                                      cAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCt                                         >  1:1917607/1‑150 (MQ=255)
                                                                                                        aGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCAGGCCTACATGCCGGATACTGAATAATAGGGGTGGATTCTAACGACGGTTAGCTTAATTGTCATTCATCAGTATCAGTAAAGTTTTTACGCGTTTTTACCTGCGCCATGCttt                                       <  1:1195860/150‑1 (MQ=255)
                                                                                                          gTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTcc                                     <  1:608344/150‑1 (MQ=255)
                                                                                                          gTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTcc                                     >  1:1117928/1‑150 (MQ=255)
                                                                                                           tAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                    >  2:2008266/1‑150 (MQ=255)
                                                                                                            aGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                   >  1:427424/1‑150 (MQ=255)
                                                                                                            aGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCAGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                   <  2:1909058/150‑1 (MQ=255)
                                                                                                             gCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGa                                  >  1:1495488/1‑150 (MQ=255)
                                                                                                              ccTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGaa                                 <  2:1294691/150‑1 (MQ=255)
                                                                                                                 ccAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATAc                              >  1:1154120/1‑150 (MQ=255)
                                                                                                                    ggCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGt                           <  1:221574/150‑1 (MQ=255)
                                                                                                                      ccATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGa                         >  1:31863/1‑150 (MQ=255)
                                                                                                                           gTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGtt                    >  2:1169232/1‑150 (MQ=255)
                                                                                                                               tGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                <  2:1952702/150‑1 (MQ=255)
                                                                                                                               tGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                <  2:1418202/150‑1 (MQ=255)
                                                                                                                               tGTCGTGCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGAAGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGCTAGTTTTCg                <  2:204487/150‑1 (MQ=255)
                                                                                                                                  cGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGaaa             >  1:628696/1‑150 (MQ=255)
                                                                                                                                  cGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGaaa             >  1:1018474/1‑150 (MQ=255)
                                                                                                                                  cGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGGTTTTACCTGCGCCATGCTTTCCTGAATACGGTGATAGTTTTCGaaa             >  2:244574/1‑150 (MQ=255)
                                                                                                                                   tttcaggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAAc            >  1:854439/7‑150 (MQ=255)
                                                                                                                                   tttcaggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAAc            >  2:1094806/7‑150 (MQ=255)
                                                                                                                                   gTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAAc            >  1:1596902/1‑150 (MQ=255)
                                                                                                                                   gTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAAc            <  1:1199489/150‑1 (MQ=255)
                                                                                                                                    ttCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACg           <  1:1782672/150‑1 (MQ=255)
                                                                                                                                     tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg          >  2:577708/1‑150 (MQ=255)
                                                                                                                                     tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg          >  1:535607/1‑150 (MQ=255)
                                                                                                                                     tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg          >  2:1238044/1‑150 (MQ=255)
                                                                                                                                      ccagATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg         >  1:1782751/4‑150 (MQ=255)
                                                                                                                                      ccTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg         <  1:1786454/150‑1 (MQ=255)
                                                                                                                                      ccTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg         <  2:873760/150‑1 (MQ=255)
                                                                                                                                       cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt        >  1:2002800/1‑150 (MQ=255)
                                                                                                                                           tCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTcc    <  2:1049977/150‑1 (MQ=255)
                                                                                                                                            ccGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCcg   >  2:1853752/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGC  >  NC_000913/4390256‑4390546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: