Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,415,525 G→T 7.1% E417D (GAG→GAT) ‡ aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,5250GT 7.1% 286.9 / 10.5 99E417D (GAG→GAT) ‡aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (47/45);  new base T (3/4);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01

Only 100 of 107 total aligned reads displayed.

GCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGG  >  NC_000913/4415376‑4415673
                                                                                                                                                     |                                                                                                                                                    
 ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                                                                                                                                                     >  1:598135/1‑150 (MQ=255)
 ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                                                                                                                                                     >  1:1059761/1‑150 (MQ=255)
 ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                                                                                                                                                     >  1:1188138/1‑150 (MQ=255)
 ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                                                                                                                                                     >  2:1748811/1‑150 (MQ=255)
 ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                                                                                                                                                     >  1:1653906/1‑150 (MQ=255)
 ccAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGATATTGCGAGGAGAGTCAGCTGCAGCGGCTTTACCGGgaga                                                                                                                                                     >  2:137860/1‑150 (MQ=255)
  cAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGAt                                                                                                                                                    >  1:8884/1‑150 (MQ=255)
   aaaGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATg                                                                                                                                                   <  2:581311/150‑1 (MQ=255)
     agaagCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGcc                                                                                                                                                 <  1:1437710/150‑1 (MQ=255)
        agCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGt                                                                                                                                              <  2:1766731/150‑1 (MQ=255)
         gCCCTGTGGGCGCGTATATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTa                                                                                                                                             >  2:505845/1‑150 (MQ=255)
          cccTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTaa                                                                                                                                            >  1:1112642/1‑150 (MQ=255)
           ccTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCATAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTTTTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTaaa                                                                                                                                           >  2:138194/1‑150 (MQ=255)
            cTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAAc                                                                                                                                          <  1:1939674/150‑1 (MQ=255)
            cTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaag                                                                                                                                          <  1:783156/150‑2 (MQ=255)
             tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                         <  2:605114/150‑3 (MQ=255)
             tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                         <  2:601985/150‑3 (MQ=255)
             tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                         <  2:1976940/150‑3 (MQ=255)
              gtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAg                                                                                                                                        <  2:1750099/150‑1 (MQ=255)
                    gcgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTg                                                                                                                                  >  1:680433/1‑150 (MQ=255)
                     cgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTgg                                                                                                                                 >  1:319486/1‑150 (MQ=255)
                     cgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTgg                                                                                                                                 >  1:1437261/1‑150 (MQ=255)
                      gTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTggg                                                                                                                                <  1:611806/150‑1 (MQ=255)
                      gTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCGGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTggg                                                                                                                                <  1:603847/150‑1 (MQ=255)
                          atttaCGCCTGCGTCAATCTTTTTTCTCTGCAACACAGGTATGCCGTTTGTGGCCGAAGCGAGGGCGGTGCTGGTCGTCATTGGTTATTGCGAGGAGCGTGAGCTGACGCGGCTTTACCGGGAGATGCCGGTAAACAGAATTTGGGAAgg                                                                                                                            <  2:1534430/150‑1 (MQ=255)
                           tttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGt                                                                                                                           >  1:659870/1‑150 (MQ=255)
                             taCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTc                                                                                                                         <  2:1995232/150‑1 (MQ=255)
                                gCCGGCGGCAAAATTTGTGATCTGCACAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCGGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCTgg                                                                                                                      <  2:1362267/150‑1 (MQ=255)
                                 ccTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGc                                                                                                                     <  2:960859/150‑1 (MQ=255)
                                  cTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCa                                                                                                                    >  1:262309/1‑150 (MQ=255)
                                    gCGGCAAAATTTGTGATCTGCAAAAGAGGTTTGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAat                                                                                                                  <  1:936581/150‑1 (MQ=255)
                                       gCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtatt                                                                                                               >  1:172278/1‑150 (MQ=255)
                                        cAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtatta                                                                                                              >  1:1306294/1‑150 (MQ=255)
                                        cAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtatta                                                                                                              >  2:571237/1‑150 (MQ=255)
                                          aaaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtg                                                                                                            <  2:919853/150‑1 (MQ=255)
                                          aaaTTGGTGATCTGCAAAAGAGGTATGCCGTTGGGGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtg                                                                                                            <  1:258745/150‑1 (MQ=255)
                                           aaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                           <  1:1972779/150‑1 (MQ=255)
                                                gtgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTg                                                                                                      <  2:1882967/150‑1 (MQ=255)
                                                gtgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGACGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGACTg                                                                                                      >  2:358922/1‑150 (MQ=255)
                                                 tgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTgg                                                                                                     >  2:1846929/1‑150 (MQ=255)
                                                  gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                    >  2:429064/1‑150 (MQ=255)
                                                  gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                    <  2:659870/150‑1 (MQ=255)
                                                  gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                    >  1:1952828/1‑150 (MQ=255)
                                                   aTCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAt                                                                                                   >  1:866192/1‑150 (MQ=255)
                                                    tCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtg                                                                                                  >  1:1366671/1‑150 (MQ=255)
                                                    tCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtg                                                                                                  <  1:675691/150‑1 (MQ=255)
                                                        cAAAAGAGGTATGCCTTTTGTGGCCGAAGCGATGGACGTGATGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTCGATGTGtt                                                                                              >  1:1910233/1‑150 (MQ=255)
                                                        cAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGtt                                                                                              >  1:926738/1‑150 (MQ=255)
                                                         taaaGAGGTTTGCGGTTTGGCGCCGAAGCGATGGTGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTATACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTg                                                                                             <  1:329917/149‑1 (MQ=255)
                                                         aaaaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCAGTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAGACAGTATTTCGGAAGGTTCCGGCAAAAATATGTGCCTGGAAgtgtat                                                                                             >  1:322585/1‑148 (MQ=255)
                                                           aaGACGTATGCCGTATGTGGCCGAAGCTATGGAGGCGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTATACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTgcg                                                                                           <  2:987887/150‑1 (MQ=255)
                                                                gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtc                                                                                      >  1:563851/1‑150 (MQ=255)
                                                                 tATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtct                                                                                     <  2:28681/150‑1 (MQ=255)
                                                                  ttGCCGGTGGTGGCCGTAGCGCTGGCGGGGGTGGGCGGGAGTGGGTTTTGCGAGGAGAGTGAGGTGCCGCGGGTTTACCGGGTGATGCCGGTAAACAGTATTTGGTATGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtctc                                                                                    <  1:813059/149‑1 (MQ=255)
                                                                  attccGTGTGTGGCCGACTCGCGGGCGGTGCGGGGCGGCCTGGGGTATTGCGAGGAGATTGAGCTGCCGCGTCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGtctc                                                                                    <  2:1476721/147‑1 (MQ=255)
                                                                    gCCGTTTGGGGCCGAAGCGAGGGAGGTGCTGGGCGGCTTTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGTTGCCGGTAAACAGTATTTGGGAAGGATCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCaa                                                                                  <  1:1495313/150‑1 (MQ=255)
                                                                     ccGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAAt                                                                                 >  2:1556451/1‑150 (MQ=255)
                                                                        tttGTGGCCGACGCGATGGAGGGGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGACTGGATGTGTTGCGCGTTCTCAATaag                                                                              <  1:1919202/150‑1 (MQ=255)
                                                                        tttGTGGCCGAAGCGATGGAGGTGCTTGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaag                                                                              <  1:1977067/150‑1 (MQ=255)
                                                                        tttGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGTCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaag                                                                              <  1:2009514/150‑1 (MQ=255)
                                                                         ttGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagc                                                                             <  2:580024/150‑1 (MQ=255)
                                                                           gtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagcaa                                                                           >  2:828959/1‑150 (MQ=255)
                                                                             ggCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagcacgc                                                                         >  2:746787/1‑150 (MQ=255)
                                                                               ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                       >  1:1766874/1‑150 (MQ=255)
                                                                               ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                       >  2:663452/1‑150 (MQ=255)
                                                                                    gCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGta                                                                  <  2:1059760/150‑1 (MQ=255)
                                                                                      gATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGtata                                                                <  1:875310/150‑1 (MQ=255)
                                                                                                    gcggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCgg                                                  >  1:886125/1‑150 (MQ=255)
                                                                                                     cggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATGAGCAAGCGGGCGTATACGACTTATTGTCGGa                                                 <  2:1842569/150‑1 (MQ=255)
                                                                                                     cggcATTGGTTATTGCGAGGAGAGTGAGCGGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGa                                                 <  2:2044300/150‑1 (MQ=255)
                                                                                                      ggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGaa                                                <  2:695978/150‑1 (MQ=255)
                                                                                                       gcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAg                                               >  1:1273498/1‑150 (MQ=255)
                                                                                                       gcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTAGGAAg                                               >  2:703352/1‑150 (MQ=255)
                                                                                                       gcATTGGTTATTGAGAGTAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAg                                               <  1:137860/150‑1 (MQ=255)
                                                                                                          ttGGGTATTGCGAGGAGAGTGAGCTGCCGCGGCGTTACCGGGCGATGCCGGTAAACAGTAGTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCAt                                            <  2:551598/150‑1 (MQ=255)
                                                                                                           tGGTTATTGCGCGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTCCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCAtt                                           <  2:319486/150‑1 (MQ=255)
                                                                                                            ggTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCAttt                                          >  1:695209/1‑150 (MQ=255)
                                                                                                             gTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTAGCGGGAGATGCCGGTAAACAGTATTTGGGTAGGTTCCGGCAAGATTAAGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtg                                         <  2:411640/150‑1 (MQ=255)
                                                                                                               tATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtgtg                                       <  2:1915459/150‑1 (MQ=255)
                                                                                                               tATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtgtg                                       >  2:876494/1‑150 (MQ=255)
                                                                                                                 ttGCGAGGAGAGTGAGATGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGTGAAGGTTCCGGCTATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGa                                     >  2:1709525/1‑150 (MQ=255)
                                                                                                                   gCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAg                                   >  2:1666583/1‑150 (MQ=255)
                                                                                                                     gatgagaGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTTAACAGTATTTGGGAAGGTGCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAATCATTTGTGGAAGTg                                 <  2:559114/147‑1 (MQ=255)
                                                                                                                       ggagAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGaa                               >  1:12122/1‑150 (MQ=255)
                                                                                                                            gTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGc                          >  1:1162877/1‑150 (MQ=255)
                                                                                                                              gAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAg                        >  2:1291169/1‑150 (MQ=255)
                                                                                                                                 cTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTTTTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGGATTTGTGGAAGTGATAGGGGAGGAt                     >  1:369889/1‑150 (MQ=255)
                                                                                                                                   gCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                   <  2:598135/150‑1 (MQ=255)
                                                                                                                                   gCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                   >  2:853228/1‑150 (MQ=255)
                                                                                                                                    ccGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGc                  <  1:535369/150‑1 (MQ=255)
                                                                                                                                        ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAATGGGCTGGAGGGCTagt              >  2:118544/4‑148 (MQ=255)
                                                                                                                                        ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt              >  2:236232/4‑150 (MQ=255)
                                                                                                                                        ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt              >  1:458656/4‑150 (MQ=255)
                                                                                                                                          cTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt            >  2:444503/1‑150 (MQ=255)
                                                                                                                                           tttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTg           <  2:1306293/150‑1 (MQ=255)
                                                                                                                                             tACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCTATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt         >  2:1338723/1‑150 (MQ=255)
                                                                                                                                             tACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt         >  2:946121/1‑150 (MQ=255)
                                                                                                                                             tACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGAGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt         >  2:957772/1‑150 (MQ=255)
                                                                                                                                               ccGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcg       <  2:8884/150‑1 (MQ=255)
                                                                                                                                                    agaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGAGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATAGCTATTTTGATCGCGCgg  >  1:18314/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGG  >  NC_000913/4415376‑4415673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: