Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,415,528 G→C 6.9% M418I (ATG→ATC) ‡ aidB → DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,5280GC 6.9% 308.8 / 11.1 99M418I (ATG→ATC) ‡aidBDNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (48/44);  new base C (3/4);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 101 total aligned reads displayed.

AAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCG  >  NC_000913/4415379‑4415677
                                                                                                                                                     |                                                                                                                                                     
aaaGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATg                                                                                                                                                       <  2:581311/150‑1 (MQ=255)
  agaagCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGcc                                                                                                                                                     <  1:1437710/150‑1 (MQ=255)
     agCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGt                                                                                                                                                  <  2:1766731/150‑1 (MQ=255)
      gCCCTGTGGGCGCGTATATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTa                                                                                                                                                 >  2:505845/1‑150 (MQ=255)
       cccTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTaa                                                                                                                                                >  1:1112642/1‑150 (MQ=255)
        ccTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCATAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTTTTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTaaa                                                                                                                                               >  2:138194/1‑150 (MQ=255)
         cTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAAc                                                                                                                                              <  1:1939674/150‑1 (MQ=255)
         cTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaag                                                                                                                                              <  1:783156/150‑2 (MQ=255)
          tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                             <  2:601985/150‑3 (MQ=255)
          tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                             <  2:605114/150‑3 (MQ=255)
          tgtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGCATCTCCCGGTaaagc                                                                                                                                             <  2:1976940/150‑3 (MQ=255)
           gtgGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAg                                                                                                                                            <  2:1750099/150‑1 (MQ=255)
                 gcgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTg                                                                                                                                      >  1:680433/1‑150 (MQ=255)
                  cgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTgg                                                                                                                                     >  1:1437261/1‑150 (MQ=255)
                  cgTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTgg                                                                                                                                     >  1:319486/1‑150 (MQ=255)
                   gTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTggg                                                                                                                                    <  1:611806/150‑1 (MQ=255)
                   gTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCGGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTggg                                                                                                                                    <  1:603847/150‑1 (MQ=255)
                       atttaCGCCTGCGTCAATCTTTTTTCTCTGCAACACAGGTATGCCGTTTGTGGCCGAAGCGAGGGCGGTGCTGGTCGTCATTGGTTATTGCGAGGAGCGTGAGCTGACGCGGCTTTACCGGGAGATGCCGGTAAACAGAATTTGGGAAgg                                                                                                                                <  2:1534430/150‑1 (MQ=255)
                        tttaCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGt                                                                                                                               >  1:659870/1‑150 (MQ=255)
                          tccGCCTGCGGCAAAATTTGTGATCTGCACAAGAGGTATGCCGTTTGTGTCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTc                                                                                                                             <  1:394515/148‑1 (MQ=255)
                          taCGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTc                                                                                                                             <  2:1995232/150‑1 (MQ=255)
                             gCCGGCGGCAAAATTTGTGATCTGCACAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCGGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCTgg                                                                                                                          <  2:1362267/150‑1 (MQ=255)
                              ccTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGc                                                                                                                         <  2:960859/150‑1 (MQ=255)
                               cTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCa                                                                                                                        >  1:262309/1‑150 (MQ=255)
                                 gCGGCAAAATTTGTGATCTGCAAAAGAGGTTTGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAat                                                                                                                      <  1:936581/150‑1 (MQ=255)
                                    gCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtatt                                                                                                                   >  1:172278/1‑150 (MQ=255)
                                     cAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtatta                                                                                                                  >  2:571237/1‑150 (MQ=255)
                                     cAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAAtatta                                                                                                                  >  1:1306294/1‑150 (MQ=255)
                                       aaaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtg                                                                                                                <  2:919853/150‑1 (MQ=255)
                                       aaaTTGGTGATCTGCAAAAGAGGTATGCCGTTGGGGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtg                                                                                                                <  1:258745/150‑1 (MQ=255)
                                        aaTTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTAtgt                                                                                                               <  1:1972779/150‑1 (MQ=255)
                                          tttGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGc                                                                                                             <  1:1830290/150‑1 (MQ=255)
                                             gtgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTg                                                                                                          <  2:1882967/150‑1 (MQ=255)
                                             gtgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGACGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGACTg                                                                                                          >  2:358922/1‑150 (MQ=255)
                                              tgATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTgg                                                                                                         >  2:1846929/1‑150 (MQ=255)
                                               gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                        >  1:1952828/1‑150 (MQ=255)
                                               gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                        >  2:429064/1‑150 (MQ=255)
                                               gATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGa                                                                                                        <  2:659870/150‑1 (MQ=255)
                                                aTCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAt                                                                                                       >  1:866192/1‑150 (MQ=255)
                                                 tCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtg                                                                                                      <  1:675691/150‑1 (MQ=255)
                                                 tCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGAtg                                                                                                      >  1:1366671/1‑150 (MQ=255)
                                                     cAAAAGAGGTATGCCTTTTGTGGCCGAAGCGATGGACGTGATGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTCGATGTGtt                                                                                                  >  1:1910233/1‑150 (MQ=255)
                                                     cAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGtt                                                                                                  >  1:926738/1‑150 (MQ=255)
                                                      taaaGAGGTTTGCGGTTTGGCGCCGAAGCGATGGTGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTATACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTg                                                                                                 <  1:329917/149‑1 (MQ=255)
                                                      aaaaGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCAGTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAGACAGTATTTCGGAAGGTTCCGGCAAAAATATGTGCCTGGAAgtgtat                                                                                                 >  1:322585/1‑148 (MQ=255)
                                                        aaGACGTATGCCGTATGTGGCCGAAGCTATGGAGGCGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTATACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTgcg                                                                                               <  2:987887/150‑1 (MQ=255)
                                                             gTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtc                                                                                          >  1:563851/1‑150 (MQ=255)
                                                              tATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtct                                                                                         <  2:28681/150‑1 (MQ=255)
                                                               ttGCCGGTGGTGGCCGTAGCGCTGGCGGGGGTGGGCGGGAGTGGGTTTTGCGAGGAGAGTGAGGTGCCGCGGGTTTACCGGGTGATGCCGGTAAACAGTATTTGGTATGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTtctc                                                                                        <  1:813059/149‑1 (MQ=255)
                                                               attccGTGTGTGGCCGACTCGCGGGCGGTGCGGGGCGGCCTGGGGTATTGCGAGGAGATTGAGCTGCCGCGTCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGtctc                                                                                        <  2:1476721/147‑1 (MQ=255)
                                                                 gCCGTTTGGGGCCGAAGCGAGGGAGGTGCTGGGCGGCTTTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGTTGCCGGTAAACAGTATTTGGGAAGGATCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCaa                                                                                      <  1:1495313/150‑1 (MQ=255)
                                                                  ccGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAAt                                                                                     >  2:1556451/1‑150 (MQ=255)
                                                                     tttGTGGCCGACGCGATGGAGGGGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGACTGGATGTGTTGCGCGTTCTCAATaag                                                                                  <  1:1919202/150‑1 (MQ=255)
                                                                     tttGTGGCCGAAGCGATGGAGGTGCTTGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaag                                                                                  <  1:1977067/150‑1 (MQ=255)
                                                                     tttGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGTCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaag                                                                                  <  1:2009514/150‑1 (MQ=255)
                                                                      ttGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagc                                                                                 <  2:580024/150‑1 (MQ=255)
                                                                        gtgGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagcaa                                                                               >  2:828959/1‑150 (MQ=255)
                                                                          ggCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATaagcacgc                                                                             >  2:746787/1‑150 (MQ=255)
                                                                            ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                           >  2:663452/1‑150 (MQ=255)
                                                                            ccGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCgg                                                                           >  1:1766874/1‑150 (MQ=255)
                                                                               aaGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCg                                                                        >  2:304948/1‑150 (MQ=255)
                                                                                 gCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGta                                                                      <  2:1059760/150‑1 (MQ=255)
                                                                                   gATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGtata                                                                    <  1:875310/150‑1 (MQ=255)
                                                                                                 gcggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCgg                                                      >  1:886125/1‑150 (MQ=255)
                                                                                                 gcggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCgg                                                      >  2:623596/1‑150 (MQ=255)
                                                                                                  cggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATGAGCAAGCGGGCGTATACGACTTATTGTCGGa                                                     <  2:1842569/150‑1 (MQ=255)
                                                                                                  cggcATTGGTTATTGCGAGGAGAGTGAGCGGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGa                                                     <  2:2044300/150‑1 (MQ=255)
                                                                                                   ggcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGaa                                                    <  2:695978/150‑1 (MQ=255)
                                                                                                    gcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAg                                                   >  1:1273498/1‑150 (MQ=255)
                                                                                                    gcATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTAGGAAg                                                   >  2:703352/1‑150 (MQ=255)
                                                                                                    gcATTGGTTATTGAGAGTAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAg                                                   <  1:137860/150‑1 (MQ=255)
                                                                                                       ttGGGTATTGCGAGGAGAGTGAGCTGCCGCGGCGTTACCGGGCGATGCCGGTAAACAGTAGTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCAt                                                <  2:551598/150‑1 (MQ=255)
                                                                                                        tGGTTATTGCGCGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTCCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCAtt                                               <  2:319486/150‑1 (MQ=255)
                                                                                                         ggTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCAttt                                              >  1:695209/1‑150 (MQ=255)
                                                                                                          gTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTAGCGGGAGATGCCGGTAAACAGTATTTGGGTAGGTTCCGGCAAGATTAAGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtg                                             <  2:411640/150‑1 (MQ=255)
                                                                                                            tATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtgtg                                           <  2:1915459/150‑1 (MQ=255)
                                                                                                            tATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTtgtg                                           >  2:876494/1‑150 (MQ=255)
                                                                                                              ttGCGAGGAGAGTGAGATGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGTGAAGGTTCCGGCTATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGa                                         >  2:1709525/1‑150 (MQ=255)
                                                                                                                gCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAg                                       >  2:1666583/1‑150 (MQ=255)
                                                                                                                  gatgagaGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTTAACAGTATTTGGGAAGGTGCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAATCATTTGTGGAAGTg                                     <  2:559114/147‑1 (MQ=255)
                                                                                                                  gaggagAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGtt                                     >  2:1649696/1‑149 (MQ=255)
                                                                                                                    ggagAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGaa                                   >  1:12122/1‑150 (MQ=255)
                                                                                                                         gTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGc                              >  1:1162877/1‑150 (MQ=255)
                                                                                                                           gAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAg                            >  2:1291169/1‑150 (MQ=255)
                                                                                                                              cTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTTTTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGGATTTGTGGAAGTGATAGGGGAGGAt                         >  1:369889/1‑150 (MQ=255)
                                                                                                                                gCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                       <  2:598135/150‑1 (MQ=255)
                                                                                                                                gCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCg                       >  2:853228/1‑150 (MQ=255)
                                                                                                                                 ccGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGGTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGc                      <  1:535369/150‑1 (MQ=255)
                                                                                                                                     ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAATGGGCTGGAGGGCTagt                  >  2:118544/4‑148 (MQ=255)
                                                                                                                                     ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt                  >  2:236232/4‑150 (MQ=255)
                                                                                                                                     ctgtttACCGGCATCTCCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtt                  >  1:458656/4‑150 (MQ=255)
                                                                                                                                       cTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTAtttt                >  2:444503/1‑150 (MQ=255)
                                                                                                                                        tttACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTg               <  2:1306293/150‑1 (MQ=255)
                                                                                                                                          tACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCTATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt             >  2:1338723/1‑150 (MQ=255)
                                                                                                                                          tACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt             >  2:946121/1‑150 (MQ=255)
                                                                                                                                          tACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGAGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGAt             >  2:957772/1‑150 (MQ=255)
                                                                                                                                            ccGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATcg           <  2:8884/150‑1 (MQ=255)
                                                                                                                                                 agaTGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGAGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATAGCTATTTTGATCGCGCgg      >  1:18314/1‑150 (MQ=255)
                                                                                                                                                    tGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTtc   >  1:1179836/1‑150 (MQ=255)
                                                                                                                                                     gCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTtcg  <  1:891109/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAAGGTTCCGGCAATATTATGTGCCTGGATGTGTTGCGCGTTCTCAATAAGCAAGCGGGCGTATACGACTTATTGTCGGAAGCATTTGTGGAAGTGAAAGGGCAGGATCGCTATTTTGATCGCGCGGTTCG  >  NC_000913/4415379‑4415677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: