Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 696,793 | T→A | 23.6% | noncoding (38/75 nt) | glnW ← | tRNA‑Gln |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 696,793 | 0 | T | A | 23.6% | 47.3 / 9.1 | 29 | noncoding (38/75 nt) | glnW | tRNA‑Gln |
Reads supporting (aligned to +/- strand): ref base T (14/8); new base A (4/3); total (18/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCC > NC_000913/696648‑696938 | cGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCa > 2:1672403/1‑150 (MQ=255) gATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaa > 2:1468904/1‑150 (MQ=255) gATACATTCAGAATTTGGTGGCTACGACGGGATTCGAAACTGTGACACCATCATTATGAGTGATGTGCTCTAACCAACTGAGATACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTAtgca > 2:325129/1‑147 (MQ=255) aTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTATTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc > 1:415261/1‑150 (MQ=255) ttCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg > 1:1737040/1‑150 (MQ=255) cAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt > 1:140292/1‑150 (MQ=255) aGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg < 1:437397/150‑1 (MQ=255) gAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTgg > 1:522956/1‑149 (MQ=255) aTTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCt < 2:1315579/150‑1 (MQ=255) cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCaaa > 1:723458/1‑149 (MQ=255) gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATa < 1:464608/150‑1 (MQ=255) gACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCATCGAATGCCCGAAACTAAAACCGGAGGATAACAGGTAGGCGATACACCAATAAACGGGCg > 1:446201/1‑150 (MQ=21) catTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCTGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCg < 2:1533281/150‑1 (MQ=255) tGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCga < 2:494211/150‑1 (MQ=255) aaCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAACACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAACGAGCGGTGAACGGCGTAAGCTAAGAAGATCGCt > 1:1971069/1‑150 (MQ=21) cTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCACAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTa < 1:1701748/150‑1 (MQ=255) ctcggcgCCAGTTTCTGTCCTCGATTGCGGGGGTACTTCGAGTCGTCCTAGGTAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa < 1:1776517/144‑1 (MQ=21) aCGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAAACACCGCTGAAACGATAAATCTAAGAAAATCGATGGTGTACCTCGAt > 2:1809795/1‑150 (MQ=14) gTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCGTACTCGAAGAAGATGGCTGGGGTACCTGGATTc > 1:679624/1‑150 (MQ=255) ttGTTTCTTAGATGGCTTTTCTACCTGGATTCGACACAGGTAATGACGGTATCAATTTCCGGTGTCTTACTGATTGTCGATACCTAAATAACTGTGCGTTGAAGCGATTACTTTAATACGATGGCTGGGGTACCTGGATTCGAACCAggg < 2:931108/150‑1 (MQ=255) ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGAAACGCGTAGTCGAAGAAGATGGATCGGGTACCTGGATTCCATACAGGGAAAGGATGTATCAAATACCGGttcgtaac > 2:1814698/1‑143 (MQ=18) aCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGATGATGGCTGGGTTACCTGGATTCGAACCAGGGAAGTCCGGTATCATAAACCGGTTCCTTACCGCtt > 2:1822243/1‑150 (MQ=37) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc < 2:140392/65‑1 (MQ=14) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc > 1:140392/3‑67 (MQ=14) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg > 2:1118014/3‑77 (MQ=17) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg < 1:1118015/75‑1 (MQ=17) ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGAACCGGGTACTCGAAGATGATGGCTGGGGTACCTGGATACGAACCAGGCGATGCCGGAATCAACTGCGGGTTCGTTACAGCTGGGCGATAcccc > 2:1169697/1‑150 (MQ=25) ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaa < 1:80498/71‑1 (MQ=18) ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaa > 2:80498/1‑71 (MQ=18) | CGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCC > NC_000913/696648‑696938 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |