Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 696,793 T→A 23.6% noncoding (38/75 nt) glnW ← tRNA‑Gln

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,7930TA23.6% 47.3 / 9.1 29noncoding (38/75 nt)glnWtRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base T (14/8);  new base A (4/3);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCC  >  NC_000913/696648‑696938
                                                                                                                                                 |                                                                                                                                                 
cGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCa                                                                                                                                               >  2:1672403/1‑150 (MQ=255)
 gATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaa                                                                                                                                              >  2:1468904/1‑150 (MQ=255)
 gATACATTCAGAATTTGGTGGCTACGACGGGATTCGAAACTGTGACACCATCATTATGAGTGATGTGCTCTAACCAACTGAGATACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTAtgca                                                                                                                                              >  2:325129/1‑147 (MQ=255)
      aTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTATTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc                                                                                                                                         >  1:415261/1‑150 (MQ=255)
       ttCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                                                                                                        >  1:1737040/1‑150 (MQ=255)
         cAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                                                                                                      >  1:140292/1‑150 (MQ=255)
          aGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg                                                                                                                                     <  1:437397/150‑1 (MQ=255)
           gAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTgg                                                                                                                                    >  1:522956/1‑149 (MQ=255)
             aTTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCt                                                                                                                                  <  2:1315579/150‑1 (MQ=255)
                                   cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCaaa                                                                                                            >  1:723458/1‑149 (MQ=255)
                                    gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATa                                                                                                           <  1:464608/150‑1 (MQ=255)
                                            gACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCATCGAATGCCCGAAACTAAAACCGGAGGATAACAGGTAGGCGATACACCAATAAACGGGCg                                                                                                   >  1:446201/1‑150 (MQ=21)
                                                    catTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCTGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCg                                                                                           <  2:1533281/150‑1 (MQ=255)
                                                             tGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCga                                                                                  <  2:494211/150‑1 (MQ=255)
                                                                        aaCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAACACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAACGAGCGGTGAACGGCGTAAGCTAAGAAGATCGCt                                                                       >  1:1971069/1‑150 (MQ=21)
                                                                              cTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCACAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTa                                                                 <  1:1701748/150‑1 (MQ=255)
                                                                                    ctcggcgCCAGTTTCTGTCCTCGATTGCGGGGGTACTTCGAGTCGTCCTAGGTAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa                                                           <  1:1776517/144‑1 (MQ=21)
                                                                                     aCGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAAACACCGCTGAAACGATAAATCTAAGAAAATCGATGGTGTACCTCGAt                                                          >  2:1809795/1‑150 (MQ=14)
                                                                                       gTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCGTACTCGAAGAAGATGGCTGGGGTACCTGGATTc                                                        >  1:679624/1‑150 (MQ=255)
                                                                                                ttGTTTCTTAGATGGCTTTTCTACCTGGATTCGACACAGGTAATGACGGTATCAATTTCCGGTGTCTTACTGATTGTCGATACCTAAATAACTGTGCGTTGAAGCGATTACTTTAATACGATGGCTGGGGTACCTGGATTCGAACCAggg                                               <  2:931108/150‑1 (MQ=255)
                                                                                                                             ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGAAACGCGTAGTCGAAGAAGATGGATCGGGTACCTGGATTCCATACAGGGAAAGGATGTATCAAATACCGGttcgtaac                  >  2:1814698/1‑143 (MQ=18)
                                                                                                                                  aCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGATGATGGCTGGGTTACCTGGATTCGAACCAGGGAAGTCCGGTATCATAAACCGGTTCCTTACCGCtt             >  2:1822243/1‑150 (MQ=37)
                                                                                                                                     tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                                                                             <  2:140392/65‑1 (MQ=14)
                                                                                                                                     tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc                                                                                             >  1:140392/3‑67 (MQ=14)
                                                                                                                                     tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg                                                                                   >  2:1118014/3‑77 (MQ=17)
                                                                                                                                     tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg                                                                                   <  1:1118015/75‑1 (MQ=17)
                                                                                                                                             ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGAACCGGGTACTCGAAGATGATGGCTGGGGTACCTGGATACGAACCAGGCGATGCCGGAATCAACTGCGGGTTCGTTACAGCTGGGCGATAcccc  >  2:1169697/1‑150 (MQ=25)
                                                                                                                                             ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaa                                                                                 <  1:80498/71‑1 (MQ=18)
                                                                                                                                             ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaa                                                                                 >  2:80498/1‑71 (MQ=18)
                                                                                                                                                 |                                                                                                                                                 
CGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCC  >  NC_000913/696648‑696938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: