| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 696,801 | A→T | 24.2% | noncoding (30/75 nt) | glnW ← | tRNA‑Gln |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 696,801 | 0 | A | T | 24.2% | 41.3 / 9.2 | 28 | noncoding (30/75 nt) | glnW | tRNA‑Gln |
| Reads supporting (aligned to +/- strand): ref base A (14/7); new base T (3/4); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
ATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTA > NC_000913/696654‑696945 | aTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTATTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc > 1:415261/1‑150 (MQ=255) ttCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg > 1:1737040/1‑150 (MQ=255) cAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt > 1:140292/1‑150 (MQ=255) aGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg < 1:437397/150‑1 (MQ=255) gAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTgg > 1:522956/1‑149 (MQ=255) aTTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCt < 2:1315579/150‑1 (MQ=255) cGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCaaa > 1:723458/1‑149 (MQ=255) gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATa < 1:464608/150‑1 (MQ=255) gACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCATCGAATGCCCGAAACTAAAACCGGAGGATAACAGGTAGGCGATACACCAATAAACGGGCg > 1:446201/1‑150 (MQ=21) catTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCTGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCg < 2:1533281/150‑1 (MQ=255) tGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCga < 2:494211/150‑1 (MQ=255) aaCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAACACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAACGAGCGGTGAACGGCGTAAGCTAAGAAGATCGCt > 1:1971069/1‑150 (MQ=21) cTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCACAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTa < 1:1701748/150‑1 (MQ=255) ctcggcgCCAGTTTCTGTCCTCGATTGCGGGGGTACTTCGAGTCGTCCTAGGTAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGa < 1:1776517/144‑1 (MQ=21) aCGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAAACACCGCTGAAACGATAAATCTAAGAAAATCGATGGTGTACCTCGAt > 2:1809795/1‑150 (MQ=14) gTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCGTACTCGAAGAAGATGGCTGGGGTACCTGGATTc > 1:679624/1‑150 (MQ=255) ttGTTTCTTAGATGGCTTTTCTACCTGGATTCGACACAGGTAATGACGGTATCAATTTCCGGTGTCTTACTGATTGTCGATACCTAAATAACTGTGCGTTGAAGCGATTACTTTAATACGATGGCTGGGGTACCTGGATTCGAACCAggg < 2:931108/150‑1 (MQ=255) ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGAAACGCGTAGTCGAAGAAGATGGATCGGGTACCTGGATTCCATACAGGGAAAGGATGTATCAAATACCGGttcgtaac > 2:1814698/1‑143 (MQ=18) aCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGATGATGGCTGGGTTACCTGGATTCGAACCAGGGAAGTCCGGTATCATAAACCGGTTCCTTACCGCtt > 2:1822243/1‑150 (MQ=37) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc > 1:140392/3‑67 (MQ=14) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAAc < 2:140392/65‑1 (MQ=14) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg < 1:1118015/75‑1 (MQ=17) tcggAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg > 2:1118014/3‑77 (MQ=17) ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAGCCGGGCGGTGAACCGGGTACTCGAAGATGATGGCTGGGGTACCTGGATACGAACCAGGCGATGCCGGAATCAACTGCGGGTTCGTTACAGCTGGGCGATAcccc > 2:1169697/1‑150 (MQ=25) ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaa < 1:80498/71‑1 (MQ=18) ccGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaa > 2:80498/1‑71 (MQ=18) cAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACAGCTTGGCGATACCCCATCCGTa > 1:1554148/1‑150 (MQ=255) cAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAGAAACGGGCGGTGAAGCGGTTACTCTAAGAAGGTGGCTGGGGTACCTGGATACGAAGCAGGCCCAGCCGGATAAAAAAACCGCTTCCAAACCGATGGGCGATACCGCAtaagaa > 2:974951/1‑145 (MQ=255) | ATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTA > NC_000913/696654‑696945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |