Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 794,938 C→A 8.2% intergenic (+16/‑151) acrZ → / → modA AcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,9380CA 8.2% 348.5 / 13.2 130intergenic (+16/‑151)acrZ/modAAcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein
Reads supporting (aligned to +/- strand):  ref base C (61/56);  new base A (5/7);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

AAAGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACC  >  NC_000913/794789‑795087
                                                                                                                                                     |                                                                                                                                                     
aaaGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCAACCTGGGTCTGATATACGGTCTTGGTGAAGTATTCAACATATTTTATGGTGTTTGTAAAAAAGACCATCCCGGACAAAATCAATGATTCCCTGAATGCACGc                                                                                                                                                       >  2:1276374/1‑150 (MQ=255)
 aaGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCt                                                                                                                                                      <  1:1140473/150‑1 (MQ=255)
  aGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCtt                                                                                                                                                     <  2:729435/150‑1 (MQ=255)
       ggTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCg                                                                                                                                                <  2:625252/150‑1 (MQ=255)
         tATTCTCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCGGGGGCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                              <  1:1833280/150‑1 (MQ=255)
            tCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAt                                                                                                                                           <  1:219713/150‑1 (MQ=255)
               ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttca                                                                                                                                        <  2:1765926/150‑3 (MQ=255)
               ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCACGCTTAGTCGGGCAtttt                                                                                                                                        >  1:1180311/1‑150 (MQ=255)
               ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTACCTGAATGCCCGCTTAGTCGGGCCtttt                                                                                                                                        >  2:239556/1‑150 (MQ=255)
                cGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttc                                                                                                                                       <  2:1661881/150‑1 (MQ=255)
                cGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAtttta                                                                                                                                       >  1:1385349/1‑149 (MQ=255)
                    aTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                   >  1:882650/1‑150 (MQ=255)
                     tCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttcttttt                                                                                                                                  >  2:1334206/1‑150 (MQ=255)
                      cATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttttc                                                                                                                                 <  2:1764284/150‑1 (MQ=255)
                         ggTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCATTTTCTCa                                                                                                                              >  2:251187/1‑150 (MQ=255)
                           tACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAAc                                                                                                                            >  1:1629158/1‑150 (MQ=255)
                              cTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGACCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTc                                                                                                                         >  2:2005016/1‑150 (MQ=255)
                               tGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGACTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCCGCCAGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTAGGGAATTTTCTATTTATCAACATcc                                                                                                                        >  1:75164/1‑150 (MQ=255)
                                gTCGTGATGGCCATCATCCTGGGTCTGATTAACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCt                                                                                                                       >  2:175581/1‑150 (MQ=255)
                                  cGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGc                                                                                                                     >  2:1672645/1‑150 (MQ=255)
                                  cGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCt                                                                                                                    >  2:796188/1‑150 (MQ=255)
                                        ggCCATCATCCTTGGGCTGATTTACGTTCTTGGTTAAGTATTCATCATGTTTTCTGGTGTTTGTAAAAAAGTCCAGCCCGGACAAAATCATTGATTCCCTGAATTCCAGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGGTTTTcc                                                                                                               <  2:573326/150‑1 (MQ=255)
                                          ccATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTg                                                                                                             >  2:267079/1‑150 (MQ=255)
                                           catcatCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGc                                                                                                            <  1:68845/150‑1 (MQ=255)
                                           catcatCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTACCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTATCCTGc                                                                                                            >  1:540022/1‑150 (MQ=255)
                                            atcatcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTTCTTTTCCTGcc                                                                                                           <  1:2038799/150‑1 (MQ=255)
                                              catcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGa                                                                                                         >  2:1597798/1‑150 (MQ=255)
                                                tcCTGCGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGATGGTAAAAAAGACCAGACAGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGTCATTTACTTTTTCTCAGCTTCCTGCTATTCCTGCCGatt                                                                                                       >  2:1242063/1‑149 (MQ=255)
                                                   tGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttt                                                                                                    >  1:778521/1‑150 (MQ=255)
                                                   tGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGGAAAAAAGCCCAGCCCGGACAAAATAATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCACCTTCCTGCTTTTCCTGCCGATAttt                                                                                                    >  1:1110748/1‑150 (MQ=255)
                                                     ggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTACCTGAATGAACGCTTAGTCGGGCATTTTCTATTTCTCAACTTCCTGCTTTTCCTGCCGATAatttt                                                                                                  >  2:889134/1‑150 (MQ=255)
                                                      gTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAtttttt                                                                                                 >  2:1382441/1‑150 (MQ=255)
                                                       tCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACGTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                >  2:1021526/1‑150 (MQ=255)
                                                       tCTGATTTACGGTCTTGGTGAAGTATTCAACATATTTTCTGGTGTTGGTAAAAAAGTCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                <  1:162472/150‑1 (MQ=255)
                                                        cTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               <  1:1190440/150‑1 (MQ=255)
                                                        cTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               >  1:76694/1‑150 (MQ=255)
                                                            tttACGGTCTTGGTGAAGTATTCAACATCTTTTGTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAGTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATc                                                                                           <  1:1709347/150‑1 (MQ=255)
                                                            tttACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATc                                                                                           <  2:1815041/150‑1 (MQ=255)
                                                                cGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGCCCAGCCCGGACAAAATCATTGATTACCTGAATGCCCGCTTATTCGGGCATTTTCTTTTTATCAACTTAATGATTTTCATTCAGATATTTTTTCTTATCTAcc                                                                                       >  1:895008/1‑150 (MQ=255)
                                                                cGGGCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAcc                                                                                       <  2:1565869/150‑1 (MQ=255)
                                                                  gTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTc                                                                                     <  2:1797442/150‑1 (MQ=255)
                                                                  gTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCCTTTCCTGCCGATATTTTTTCTTATCTACCTc                                                                                     <  2:804268/150‑1 (MQ=255)
                                                                      tGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACaa                                                                                 >  2:884916/1‑150 (MQ=255)
                                                                       ggTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACaaa                                                                                >  2:607311/1‑150 (MQ=255)
                                                                       ggTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACaaa                                                                                >  1:38732/1‑150 (MQ=255)
                                                                         tGAAGTATTCAACATGTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGATTGCCCGCGTAGTCGGGCATTTTCTTTTTCTCCACTTCCTGCTTTTCCTGCCGTTATTTTTTCTTATATAGCTGACAAAgg                                                                              >  2:104144/1‑150 (MQ=255)
                                                                          gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                             >  1:1514675/1‑150 (MQ=255)
                                                                          gAAGTATTCAACATCTTTTCTGGAGTTGGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                             <  2:175333/150‑1 (MQ=255)
                                                                              tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                         <  2:1710151/150‑1 (MQ=255)
                                                                                  cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAGAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     >  1:782454/1‑150 (MQ=255)
                                                                                  cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     <  2:239665/150‑1 (MQ=255)
                                                                                  cAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGACCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAAATTACTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCCCAAAGGTTAGAAATa                                                                     >  2:141373/1‑150 (MQ=255)
                                                                                     cATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCACGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCt                                                                                          >  1:583706/1‑126 (MQ=255)
                                                                                             cTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTACTGCTTTTCCTGCCGATATTTTTTCTTATATACCTCACAAAGGTTAGCAATAACTGCTGGGaa                                                          >  2:1461813/1‑150 (MQ=255)
                                                                                                gtgtTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAAt                                                       <  2:284404/150‑1 (MQ=255)
                                                                                                 tgtTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAAtt                                                      >  1:1396784/1‑150 (MQ=255)
                                                                                                   ttGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTcc                                                    <  1:447120/150‑1 (MQ=255)
                                                                                                    tGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCg                                                   <  2:1281167/150‑1 (MQ=255)
                                                                                                    tGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCg                                                   <  2:1780031/150‑1 (MQ=255)
                                                                                                    tGGTAAAAAAGACCAGCCCGGACAAAATAATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCg                                                   <  1:879944/150‑1 (MQ=255)
                                                                                                     ggTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTTGCAATAACTGCTGGGAAAATTCCGa                                                  >  1:888383/1‑150 (MQ=255)
                                                                                                     ggTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGa                                                  >  1:1926943/1‑150 (MQ=255)
                                                                                                     ggTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGa                                                  <  2:1575506/150‑1 (MQ=255)
                                                                                                      ttAAAAAAGCCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                 <  1:92891/149‑1 (MQ=255)
                                                                                                      gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACATCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                 >  1:530611/1‑150 (MQ=255)
                                                                                                       tAAAAAAGACCAGCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                               <  1:796189/150‑1 (MQ=255)
                                                                                                        aaaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGATTAGTCGGGCATTTTATTTTTCTCAACTTCCTGCTTTTCCTGCAGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                               <  2:471117/150‑1 (MQ=255)
                                                                                                            aaGACCAGCCCTGACAAAATCTTTGATTCCCTGAATGCACGATTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGACGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTc                                           <  2:776480/150‑1 (MQ=255)
                                                                                                             aGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCg                                          <  1:175581/150‑1 (MQ=255)
                                                                                                               aCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGtt                                        <  2:1038841/150‑1 (MQ=255)
                                                                                                                ccAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTta                                       <  1:991162/150‑1 (MQ=255)
                                                                                                                ccAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTACGAGTTAGTCGTta                                       >  2:1909576/1‑150 (MQ=255)
                                                                                                                ccAGCCCGGACAAAATCAGTGATTCCCTGAATGCCCGCTTAGTCGGGCAGGTTCTTTTTCTCCCCTTCCGGCTTGTCCTGCCGATATTTTTTCTTATCGACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTta                                       >  1:36443/1‑150 (MQ=255)
                                                                                                                     ccGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCATATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCTAGTTAGTCGTTATATTg                                  >  2:1562179/1‑150 (MQ=255)
                                                                                                                      cGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGATGGGAAAATTCCGAGTTAGTCGTTATAtttt                                 >  1:1961108/1‑148 (MQ=255)
                                                                                                                        gACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                               <  2:906194/150‑1 (MQ=255)
                                                                                                                            aaaTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCATGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTTCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTa                           >  2:609292/1‑150 (MQ=255)
                                                                                                                               tCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACAt                        >  2:1066465/1‑150 (MQ=255)
                                                                                                                               tCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCACAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAAATCCGAGTTAGTCGTTATATTGTCGCCTACAt                        <  2:291162/150‑1 (MQ=255)
                                                                                                                                    tcttCCCTTTATGCACGGGTATTCGGGGATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                   <  1:809351/148‑1 (MQ=255)
                                                                                                                                    gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                   >  2:1386178/1‑150 (MQ=255)
                                                                                                                                     aTTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGtt                  <  2:540022/150‑1 (MQ=255)
                                                                                                                                     aTTCCCTGAATGACCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGtt                  <  2:1629157/150‑1 (MQ=255)
                                                                                                                                      ttCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTa                 <  1:1672646/150‑1 (MQ=255)
                                                                                                                                       aaagaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTAc                >  2:490144/7‑150 (MQ=255)
                                                                                                                                       aaagaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTAc                >  2:484746/7‑150 (MQ=255)
                                                                                                                                        cccTGAATGCCCGCTTTTTCGGTCTTTTTATTTTTCTCAACTTCCTGCTTTTCCTGGCGTTATTTTTTCTAATGTTCCTCTCAAAGGTTATCAATATCTGCTGTGAAATTTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACa               <  1:1276375/150‑1 (MQ=255)
                                                                                                                                        aagaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCAGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACa               >  1:1246245/6‑150 (MQ=255)
                                                                                                                                         agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGAAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTAGCCTACATAACGTTACAt              >  1:483981/5‑150 (MQ=255)
                                                                                                                                          cTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAtt             >  2:84999/1‑150 (MQ=255)
                                                                                                                                            gAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTaa           >  1:869141/1‑150 (MQ=255)
                                                                                                                                              aTGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACATCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg         <  1:1786347/150‑1 (MQ=255)
                                                                                                                                              aTGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAACTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg         <  1:971330/150‑1 (MQ=255)
                                                                                                                                               tGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAggg        <  1:791082/150‑1 (MQ=255)
                                                                                                                                                gCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTATCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg       <  1:1334207/150‑1 (MQ=255)
                                                                                                                                                gCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTATCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGACGTTATATTGTCGCCTACATAACGTTACATTAAgggg       <  1:1696759/150‑1 (MQ=255)
                                                                                                                                                  ccGCTTAGTCGGGCTTTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACATCTCAAAGGTTAGCAATAACTGCTGGGAAAATTAATAGTTAGTCTATATATTGTCGCCTACATAACTTTACATTAAGTGGtt     >  1:333433/1‑150 (MQ=255)
                                                                                                                                                  ccGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGtt     <  2:79059/150‑1 (MQ=255)
                                                                                                                                                  ccGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGtt     <  2:1655870/150‑1 (MQ=255)
                                                                                                                                                     cTTATTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAcc  <  2:573961/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACC  >  NC_000913/794789‑795087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: