Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 864,299 A→G 5.7% I220V (ATT→GTT) ‡ fsaA → fructose‑6‑phosphate aldolase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913864,2990AG 5.7% 433.0 / 10.8 142I220V (ATT→GTT) ‡fsaAfructose‑6‑phosphate aldolase 1
Reads supporting (aligned to +/- strand):  ref base A (69/64);  new base G (5/3);  total (74/68)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 142 total aligned reads displayed.

GCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTCATCG  >  NC_000913/864151‑864447
                                                                                                                                                    |                                                                                                                                                    
gcgTCAGGCGCTGGACTGCGTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGAt                                                                                                                                                     <  2:927929/150‑1 (MQ=255)
  gTCAGGCGCGGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGAttt                                                                                                                                                   <  2:1025276/150‑1 (MQ=255)
         gcTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGa                                                                                                                                            >  1:1335356/1‑150 (MQ=255)
           tGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACg                                                                                                                                          <  1:795335/150‑1 (MQ=255)
           tGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGAGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACg                                                                                                                                          >  1:1282253/1‑150 (MQ=255)
                 gcgtACTGGCAGGAGGGCAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCAGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCCGGTTAAATCGACGTTCTGc                                                                                                                                    <  2:1737399/147‑1 (MQ=255)
                 gCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGCTTAGCTATCCAGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCCGGTTAAATCGACGTTCTGc                                                                                                                                    <  2:1227803/150‑1 (MQ=255)
                 gCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGc                                                                                                                                    >  2:103637/1‑150 (MQ=255)
                 gCTTACTGGCAGGAGGTGAAACATTTACTCTGCCCCTCGAGGTGTCACAACAGCGGAAAAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCCGGTTAACTCGACGTTCTGc                                                                                                                                    <  1:1035103/150‑1 (MQ=255)
                    tACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATc                                                                                                                                 >  2:1836906/1‑150 (MQ=255)
                       tGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTc                                                                                                                              <  2:1017338/150‑1 (MQ=255)
                          cAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATa                                                                                                                           >  2:1834185/1‑150 (MQ=255)
                                gtgAATCAATTACTCTGCCACTGGATGTGCCACAACAGAGGCTTAGCTATCCGGCGGTTGCTGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGc                                                                                                                     <  2:762485/150‑1 (MQ=255)
                                 tgAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCt                                                                                                                    <  1:474242/150‑1 (MQ=255)
                                  gAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCtg                                                                                                                   >  2:1951822/1‑150 (MQ=255)
                                       aaTTACTCTGCCACTGGAAGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGAATGGCAGGGAGCGTTTGGCAGAACGTCGATTAAACCGGACGTTCTGCATCCTCATAAATTGCTGATGAc                                                                                                              >  1:1739682/1‑150 (MQ=255)
                                          tACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTg                                                                                                           <  2:894646/150‑1 (MQ=255)
                                            ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                         >  2:137140/1‑150 (MQ=255)
                                            ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                         >  2:1682232/1‑150 (MQ=255)
                                            ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                         >  2:912857/1‑150 (MQ=255)
                                              ctGCCACGGGATGGGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGCGCAGGACTGGCAGGGAGCGATTGGCAGAAAGTCGATTTACCCGGACGGTCTGCATCCTCAGTGAATGCTGATGCCTTCGCgg                                                                                                       >  2:1817005/1‑150 (MQ=255)
                                                gCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAg                                                                                                     <  1:872458/150‑1 (MQ=255)
                                                   aCTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGc                                                                                                  <  2:590367/150‑1 (MQ=255)
                                                    cttgATGTGGCACAACTGTTGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGTACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGcc                                                                                                 <  1:1999690/147‑1 (MQ=255)
                                                    cTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGcc                                                                                                 <  2:1311646/150‑1 (MQ=255)
                                                     tGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCcg                                                                                                <  1:1625650/150‑1 (MQ=255)
                                                       gATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGAAGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGTCAGATCGTCGATTTAACCGGACGTTCTGCATGCTCATAAATTGCTGATGACGTGGCGGAGTGCctcg                                                                                              >  2:180735/1‑150 (MQ=255)
                                                         tgtgGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAAAGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGAAGTGGCGGAGTGCCGCGTc                                                                                            >  1:61788/1‑150 (MQ=255)
                                                           tgGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGCCGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCta                                                                                          >  1:478201/1‑149 (MQ=255)
                                                           tgGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCtt                                                                                          >  1:166879/1‑150 (MQ=255)
                                                            ggCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTa                                                                                         >  1:353852/1‑150 (MQ=255)
                                                              cacaACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATc                                                                                       >  2:1534624/1‑150 (MQ=255)
                                                              cacaACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATc                                                                                       >  2:11582/1‑150 (MQ=255)
                                                              cacaACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATc                                                                                       >  2:2028376/1‑150 (MQ=255)
                                                              cacaACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGAGTCTTATc                                                                                       >  1:294023/1‑150 (MQ=255)
                                                                 aacaGATGAGTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAgg                                                                                    <  2:113887/150‑1 (MQ=255)
                                                                   caGATGCTTAGCTATCCGGCGGTTGATGCCGCTGTGGCTAAGTGTGAGCAGGACTGTCAGGGAGCGTTTGGCAGATCGTCTTTTTAACCGGACGTTCTGCATCCTCATATATTGCTGATGACGGGGCGGAGGGCCGCGTATTATCAGGcc                                                                                  <  1:313059/150‑1 (MQ=255)
                                                                    aGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCt                                                                                 <  1:1752212/150‑1 (MQ=255)
                                                                    aGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCt                                                                                 >  1:870571/1‑150 (MQ=255)
                                                                      atgatTAGCTATCCGGAGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTgg                                                                               <  2:1282252/150‑1 (MQ=255)
                                                                       tgatTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGa                                                                              >  2:935987/1‑150 (MQ=255)
                                                                         atTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAgg                                                                            <  2:191205/150‑1 (MQ=255)
                                                                         atTAGCTATCCGGCGGTCGATGCCGCTGTGGCGAAGGTTGAGCAGGACTGGCAGGGAGCGTGTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAgg                                                                            <  2:1382025/150‑1 (MQ=255)
                                                                           tAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTg                                                                          >  1:1039180/1‑150 (MQ=255)
                                                                             gCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATATATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                        >  1:814964/1‑150 (MQ=255)
                                                                             gCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                        <  1:1951823/150‑1 (MQ=255)
                                                                             gCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                        <  2:732957/150‑1 (MQ=255)
                                                                             gCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGGTTGGCAGAACGGCGATTTAACCGGACGTGCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                        <  1:1867193/150‑1 (MQ=255)
                                                                             gCTAGCCTGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                        <  1:979030/150‑1 (MQ=255)
                                                                               tATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCaa                                                                      >  2:461220/1‑150 (MQ=255)
                                                                               tATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCaa                                                                      >  2:602069/1‑150 (MQ=255)
                                                                                   cggcggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTAc                                                                  >  1:1666373/1‑150 (MQ=255)
                                                                                   cggcggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGGTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGGGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTAc                                                                  <  1:1575194/150‑1 (MQ=255)
                                                                                    ggcggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACt                                                                 >  1:1470980/1‑150 (MQ=255)
                                                                                    ggcggTTGATGCCGCTGAGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTAGATTAAACCGGACCTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACt                                                                 >  1:510827/1‑150 (MQ=255)
                                                                                       ggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGcc                                                              <  1:1680817/150‑1 (MQ=255)
                                                                                       ggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGcc                                                              <  1:201957/150‑1 (MQ=255)
                                                                                       ggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGcc                                                              >  1:499637/1‑150 (MQ=255)
                                                                                       ggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGcc                                                              >  1:1445209/1‑150 (MQ=255)
                                                                                          tGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGGTGGGCAGAACGGCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCcac                                                           <  2:276191/150‑1 (MQ=255)
                                                                                            aTGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCcacac                                                         <  2:1150361/150‑1 (MQ=255)
                                                                                              gCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCacacac                                                       >  1:1665928/1‑150 (MQ=255)
                                                                                              gCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCacacac                                                       >  1:1526747/1‑150 (MQ=255)
                                                                                               ccGCTGGGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGAGTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACAcc                                                      <  1:223590/150‑1 (MQ=255)
                                                                                                        cGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACAcccc                                             >  2:1091750/1‑150 (MQ=255)
                                                                                                           aGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGa                                          <  1:1212474/150‑1 (MQ=255)
                                                                                                              ttGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTa                                       >  1:626103/1‑150 (MQ=255)
                                                                                                              ttGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTAAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTa                                       <  2:261691/150‑1 (MQ=255)
                                                                                                               tGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTAc                                      <  1:1171696/150‑1 (MQ=255)
                                                                                                                gAGCAGGACTGGAAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGGGGCAATTACTGCCCACACACCTCACACCCCGGATTACg                                     <  2:1779947/150‑1 (MQ=255)
                                                                                                                 aGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGc                                    <  2:1384242/150‑1 (MQ=255)
                                                                                                                  gCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCa                                   <  1:135244/150‑1 (MQ=255)
                                                                                                                  gCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCa                                   >  2:1337203/1‑150 (MQ=255)
                                                                                                                     ggACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCa                                >  2:423626/1‑150 (MQ=255)
                                                                                                                        cTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTTGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGtt                             >  2:1503179/1‑150 (MQ=255)
                                                                                                                        cTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCGGGATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGtt                             >  1:1865739/1‑150 (MQ=255)
                                                                                                                         tGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGttt                            <  1:1925088/150‑1 (MQ=255)
                                                                                                                          ggCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTc                           <  2:1074659/150‑1 (MQ=255)
                                                                                                                          ggCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTc                           >  1:726587/1‑150 (MQ=255)
                                                                                                                           gCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCa                          >  2:1855347/1‑150 (MQ=255)
                                                                                                                           gCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCa                          <  2:2011400/150‑1 (MQ=255)
                                                                                                                            cAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCAt                         <  2:253120/150‑1 (MQ=255)
                                                                                                                            cAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGAGTACGCATCAGTTTCAt                         >  1:1032059/1‑150 (MQ=255)
                                                                                                                              gggAGCTTTTGGCAGTACGGCGATTTACCCGGAAGTTCTGCATCCTAATAAATTGCTGATGACGTGGCGGAGTGCCGCGGCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCAttt                       <  2:1004259/150‑1 (MQ=255)
                                                                                                                              gggAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCAttt                       >  2:499113/1‑150 (MQ=255)
                                                                                                                               ggAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTc                      >  2:1708988/1‑150 (MQ=255)
                                                                                                                                 aGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgc                    >  2:292517/1‑150 (MQ=255)
                                                                                                                                 aGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgc                    <  1:11582/150‑1 (MQ=255)
                                                                                                                                  gCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATATATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgcg                   <  1:1227719/150‑1 (MQ=255)
                                                                                                                                  gCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgcg                   >  1:1901295/1‑150 (MQ=255)
                                                                                                                                   cGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCATCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGACAACACACATCACAACCCGGATTTCGCATCAGTTTGATTTAGCGa                  >  1:1068915/1‑150 (MQ=255)
                                                                                                                                     ggatgCAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGaaa                >  2:505810/5‑150 (MQ=255)
                                                                                                                                        tgCAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTg             >  1:1508639/2‑150 (MQ=255)
                                                                                                                                        ggCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTg             >  2:615762/1‑150 (MQ=255)
                                                                                                                                         gCAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCCCACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGa            >  1:584185/1‑150 (MQ=255)
                                                                                                                                         gCAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTgc            >  1:2049968/1‑149 (MQ=255)
                                                                                                                                           aGAACGTAGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTCGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCTAAACTGACa          <  2:1941711/150‑1 (MQ=255)
                                                                                                                                            gAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAg         >  2:2027556/1‑150 (MQ=255)
                                                                                                                                             aaCGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAgg        <  2:166879/150‑1 (MQ=255)
                                                                                                                                               cGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACCCCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTc      >  1:1091222/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTCATCG  >  NC_000913/864151‑864447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: