Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 864,306 C→T 5.7% intergenic (+2/+74) fsaA → / ← moeB fructose‑6‑phosphate aldolase 1/molybdopterin synthase sulfurylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913864,3060CT 5.7% 444.7 / 10.2 141intergenic (+2/+74)fsaA/moeBfructose‑6‑phosphate aldolase 1/molybdopterin synthase sulfurylase
Reads supporting (aligned to +/- strand):  ref base C (69/63);  new base T (5/3);  total (74/67)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

Only 100 of 142 total aligned reads displayed.

CGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTCATCGCATCGTA  >  NC_000913/864159‑864454
                                                                                                                                                   |                                                                                                                                                    
cgcTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCgg                                                                                                                                                    <  2:1790866/150‑1 (MQ=255)
 gcTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGa                                                                                                                                                   >  1:1335356/1‑150 (MQ=255)
   tGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACg                                                                                                                                                 <  1:795335/150‑1 (MQ=255)
   tGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGAGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACg                                                                                                                                                 >  1:1282253/1‑150 (MQ=255)
        tGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTg                                                                                                                                            <  2:1297989/150‑1 (MQ=255)
         gcgtACTGGCAGGAGGGCAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCAGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCCGGTTAAATCGACGTTCTGc                                                                                                                                           <  2:1737399/147‑1 (MQ=255)
         gCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGCTTAGCTATCCAGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCCGGTTAAATCGACGTTCTGc                                                                                                                                           <  2:1227803/150‑1 (MQ=255)
         gCTTACTGGCAGGAGGTGAAACATTTACTCTGCCCCTCGAGGTGTCACAACAGCGGAAAAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCCGGTTAACTCGACGTTCTGc                                                                                                                                           <  1:1035103/150‑1 (MQ=255)
          cTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCa                                                                                                                                          <  1:938051/150‑1 (MQ=255)
            tACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATc                                                                                                                                        >  2:1008960/1‑150 (MQ=255)
            tACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATc                                                                                                                                        >  2:1836906/1‑150 (MQ=255)
               tGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTc                                                                                                                                     <  2:1017338/150‑1 (MQ=255)
                 gCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCAt                                                                                                                                   >  1:725627/1‑150 (MQ=255)
                  cAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATa                                                                                                                                  >  2:1834185/1‑150 (MQ=255)
                        gtgAATCAATTACTCAGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGc                                                                                                                            <  2:1120361/150‑1 (MQ=255)
                         tgAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCt                                                                                                                           <  1:474242/150‑1 (MQ=255)
                          gAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCtg                                                                                                                          <  1:2033646/150‑1 (MQ=255)
                          gAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCtg                                                                                                                          >  2:1951822/1‑150 (MQ=255)
                           aaTCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCtga                                                                                                                         <  1:835209/150‑1 (MQ=255)
                           aaTCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCtga                                                                                                                         >  1:1990788/1‑150 (MQ=255)
                               aaTTACTCTGCCACTGGAAGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGAATGGCAGGGAGCGTTTGGCAGAACGTCGATTAAACCGGACGTTCTGCATCCTCATAAATTGCTGATGAc                                                                                                                     >  1:1739682/1‑150 (MQ=255)
                                  tACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTg                                                                                                                  <  2:894646/150‑1 (MQ=255)
                                    ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                                >  2:1682232/1‑150 (MQ=255)
                                    ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                                >  2:137140/1‑150 (MQ=255)
                                    ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                                >  2:257789/1‑150 (MQ=255)
                                    ctctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGc                                                                                                                >  2:1171695/1‑150 (MQ=255)
                                      ctGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCgg                                                                                                              <  1:510124/150‑1 (MQ=255)
                                      ctGCCACTGGATGTGGCACAACAGAGGATTAGCTATCCGGCGGTGGCTGCCGCTGTGGCGAAGTGTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCgg                                                                                                              <  2:561837/150‑1 (MQ=255)
                                           aCTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGc                                                                                                         <  2:590367/150‑1 (MQ=255)
                                           aCTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGc                                                                                                         >  1:612187/1‑150 (MQ=255)
                                           aCTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTCCATCCTCATAAATTGCTGATGACGTGGCGGAGTGc                                                                                                         <  2:1823129/150‑1 (MQ=255)
                                            cttgATGTGGCACAACTGTTGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGTACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGcc                                                                                                        <  1:1999690/147‑1 (MQ=255)
                                            cTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGcc                                                                                                        <  2:1311646/150‑1 (MQ=255)
                                               gATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGAAGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGTCAGATCGTCGATTTAACCGGACGTTCTGCATGCTCATAAATTGCTGATGACGTGGCGGAGTGCctcg                                                                                                     >  2:180735/1‑150 (MQ=255)
                                                 tgtgGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAAAGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGAAGTGGCGGAGTGCCGCGTc                                                                                                   >  1:61788/1‑150 (MQ=255)
                                                   tgGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGCCGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCta                                                                                                 >  1:478201/1‑149 (MQ=255)
                                                   tgGCACAACAGATGATTAGCTATCCCGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGCGAGCGTTAGGCAGAACGTCGATTTAACCGCACGTTCGGAATCCTCATAAATTGCTGATGACTTGCAGCAGTGCCGCGTCtt                                                                                                 >  2:858492/1‑150 (MQ=255)
                                                   gggCACAACAGCTGATTAGCTATCCTGCGGTTGATGGCGCGGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACGGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCtt                                                                                                 <  2:1858582/149‑1 (MQ=255)
                                                      cacaACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGCCGTGGCGGAGTGCCGCGTCTTATc                                                                                              >  2:1047405/1‑150 (MQ=255)
                                                      cacaACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATc                                                                                              >  2:2028376/1‑150 (MQ=255)
                                                         aacaGATGAGTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAgg                                                                                           <  2:113887/150‑1 (MQ=255)
                                                           caGATGCTTAGCTATCCGGCGGTTGATGCCGCTGTGGCTAAGTGTGAGCAGGACTGTCAGGGAGCGTTTGGCAGATCGTCTTTTTAACCGGACGTTCTGCATCCTCATATATTGCTGATGACGGGGCGGAGGGCCGCGTATTATCAGGcc                                                                                         <  1:313059/150‑1 (MQ=255)
                                                            aGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCt                                                                                        >  1:870571/1‑150 (MQ=255)
                                                               tgatTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGa                                                                                     >  2:935987/1‑150 (MQ=255)
                                                                 atTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAgg                                                                                   <  2:191205/150‑1 (MQ=255)
                                                                 atTAGCTATCCGGCGGTCGATGCCGCTGTGGCGAAGGTTGAGCAGGACTGGCAGGGAGCGTGTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAgg                                                                                   <  2:1382025/150‑1 (MQ=255)
                                                                   tAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTg                                                                                 >  1:1039180/1‑150 (MQ=255)
                                                                     gCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                               <  2:732957/150‑1 (MQ=255)
                                                                     gCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGGTTGGCAGAACGGCGATTTAACCGGACGTGCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGc                                                                               <  1:1867193/150‑1 (MQ=255)
                                                                           cggcggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTAc                                                                         >  1:1666373/1‑150 (MQ=255)
                                                                           cggcggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGGTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGGGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTAc                                                                         <  1:1575194/150‑1 (MQ=255)
                                                                            ggcggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACt                                                                        >  1:1470980/1‑150 (MQ=255)
                                                                            ggcggTTGATGCCGCTGAGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTAGATTAAACCGGACCTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACt                                                                        >  1:510827/1‑150 (MQ=255)
                                                                               ggTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGcc                                                                     <  1:1680817/150‑1 (MQ=255)
                                                                                    aTGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCcacac                                                                <  2:1150361/150‑1 (MQ=255)
                                                                                      gCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCacacac                                                              >  1:1665928/1‑150 (MQ=255)
                                                                                      gCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACATCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCacacac                                                              >  1:1919065/1‑150 (MQ=255)
                                                                                       ccGCTGGGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGAGTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACAcc                                                             <  1:223590/150‑1 (MQ=255)
                                                                                        cGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCt                                                            <  2:612187/150‑1 (MQ=255)
                                                                                                 gAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCg                                                   <  2:1715088/150‑1 (MQ=255)
                                                                                                  aaGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCgg                                                  <  1:411714/150‑1 (MQ=255)
                                                                                                   aGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGa                                                 <  1:1212474/150‑1 (MQ=255)
                                                                                                      ttGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTAAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTa                                              <  2:261691/150‑1 (MQ=255)
                                                                                                       tGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTAc                                             <  1:1171696/150‑1 (MQ=255)
                                                                                                          gCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCa                                          <  2:917150/150‑1 (MQ=255)
                                                                                                          gCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCa                                          <  1:135244/150‑1 (MQ=255)
                                                                                                          gCAGGACTGGCAGGGAGCGTTTGGCAGAACGGCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCa                                          <  2:1541615/150‑1 (MQ=255)
                                                                                                             ggACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCCCACACCTCACACCCCGGATTACGCATCa                                       >  2:1362682/1‑150 (MQ=255)
                                                                                                             ggACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCa                                       >  2:423626/1‑150 (MQ=255)
                                                                                                                cTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTTGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGtt                                    >  2:1503179/1‑150 (MQ=255)
                                                                                                                cTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCGGGATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGtt                                    >  1:1865739/1‑150 (MQ=255)
                                                                                                                cTGGCAGGGAGCGGTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGtt                                    <  2:277405/150‑1 (MQ=255)
                                                                                                                 tGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGttt                                   <  1:1925088/150‑1 (MQ=255)
                                                                                                                 aggCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCAGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGttt                                   >  1:1847209/2‑150 (MQ=255)
                                                                                                                  ggCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTc                                  <  2:1074659/150‑1 (MQ=255)
                                                                                                                  ggCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTc                                  >  1:726587/1‑150 (MQ=255)
                                                                                                                    cAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGAGTACGCATCAGTTTCAt                                >  1:1032059/1‑150 (MQ=255)
                                                                                                                      gggAGCTTTTGGCAGTACGGCGATTTACCCGGAAGTTCTGCATCCTAATAAATTGCTGATGACGTGGCGGAGTGCCGCGGCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCAttt                              <  2:1004259/150‑1 (MQ=255)
                                                                                                                      gggAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCAttt                              >  2:499113/1‑150 (MQ=255)
                                                                                                                         aGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgc                           <  1:11582/150‑1 (MQ=255)
                                                                                                                         aGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgc                           >  2:292517/1‑150 (MQ=255)
                                                                                                                          gCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATATATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTcgcg                          <  1:1227719/150‑1 (MQ=255)
                                                                                                                          gCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACTCCTCACACCCCGGATTACGCATCAGTTTCATTTcgcg                          <  1:1682233/150‑1 (MQ=255)
                                                                                                                           cGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCATCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGACAACACACATCACAACCCGGATTTCGCATCAGTTTGATTTAGCGa                         >  1:1068915/1‑150 (MQ=255)
                                                                                                                            gTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGAGTCTTATCAGGCCTGGAGGTTGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGaa                        <  2:1431071/150‑1 (MQ=255)
                                                                                                                                tgCAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTg                    >  1:1508639/2‑150 (MQ=255)
                                                                                                                                ggCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTg                    >  2:615762/1‑150 (MQ=255)
                                                                                                                                ggCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTg                    >  2:475189/1‑150 (MQ=255)
                                                                                                                                 gCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAACCTGa                   >  1:1523813/1‑150 (MQ=255)
                                                                                                                                 gCAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTgc                   >  1:2049968/1‑149 (MQ=255)
                                                                                                                                 ccAGAACGTCCGGTTAAATCGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTAATGCCCACACACCTCACACCCCGGATTTCGCATCAGTTTCATTTCGCGAAACTGa                   >  1:16665/2‑150 (MQ=255)
                                                                                                                                    gAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAg                <  1:1603278/150‑1 (MQ=255)
                                                                                                                                    gAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAg                >  2:2027556/1‑150 (MQ=255)
                                                                                                                                     aaCGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAgg               <  2:166879/150‑1 (MQ=255)
                                                                                                                                       cGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACCCCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTc             >  1:1091222/1‑150 (MQ=255)
                                                                                                                                         tCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATGACGCATCAGTTTCATTTCGCGAAACTGACAGGTcat           >  2:1950521/1‑150 (MQ=255)
                                                                                                                                               tAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTcatcgcatc     >  2:2022221/1‑150 (MQ=255)
                                                                                                                                                aaCCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTcatcgcatcg    <  1:1738084/150‑1 (MQ=255)
                                                                                                                                                aaCCGGACGTGCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGACCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTcatcgcatcg    <  1:302606/150‑1 (MQ=255)
                                                                                                                                                  ccGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTCATCGCATCGTa  <  1:912858/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCACAACAGATGATTAGCTATCCGGCGGTTGATGCCGCTGTGGCGAAGTTTGAGCAGGACTGGCAGGGAGCGTTTGGCAGAACGTCGATTTAACCGGACGTTCTGCATCCTCATAAATTGCTGATGACGTGGCGGAGTGCCGCGTCTTATCAGGCCTGGAGGTGGCAATTACTGCCCACACACCTCACACCCCGGATTACGCATCAGTTTCATTTCGCGAAACTGACAGGTCATCGCATCGTA  >  NC_000913/864159‑864454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: