Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 987,420 A→C 5.5% intergenic (‑438/+165) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913987,4200AC 5.5% 364.0 / 7.4 120intergenic (‑438/+165)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (57/56);  new base C (3/4);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 123 total aligned reads displayed.

CTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCT  >  NC_000913/987277‑987569
                                                                                                                                               |                                                                                                                                                     
cTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAATTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAAtt                                                                                                                                                 >  1:1335867/1‑149 (MQ=255)
  tataTTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCg                                                                                                                                               >  2:863745/1‑150 (MQ=255)
   atatTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAATTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCgg                                                                                                                                              <  2:1009008/150‑1 (MQ=255)
   atatTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCgg                                                                                                                                              <  1:1869998/150‑1 (MQ=255)
     atTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGTAt                                                                                                                                            >  2:194952/1‑150 (MQ=255)
      tttATATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGAGGAtt                                                                                                                                           >  1:863585/1‑150 (MQ=255)
      tttAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGAtt                                                                                                                                           <  1:1957177/150‑1 (MQ=255)
      tttAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGAtt                                                                                                                                           >  2:224599/1‑150 (MQ=255)
        tAAATTAACTAAACTGAAATTTTGTTTCTGATGGAAACTTTATCGACCTTGCACAAAATCTTCTTTCAGAAATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAg                                                                                                                                         <  2:470789/150‑1 (MQ=255)
        tAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATTAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAACGCGGATTAg                                                                                                                                         >  2:341665/1‑150 (MQ=255)
            ttAACTAAAAGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCATAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCATTCTTATTGCGGATTAGtttt                                                                                                                                     <  2:1760363/150‑1 (MQ=39)
             tAACTAAACGTAAATTTTGTTTCTGATGTAAACTTTATCGAACTTGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGttttt                                                                                                                                    <  2:624863/150‑1 (MQ=255)
             tAACTAAACGGAAATTTTGTTTCTGATCGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGGCAAAAAATATTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGttttt                                                                                                                                    <  2:632911/150‑1 (MQ=255)
              aaCTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTg                                                                                                                                   <  1:1998890/150‑1 (MQ=255)
                cTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTgcg                                                                                                                                 <  1:1818091/150‑1 (MQ=255)
                 tAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCATTCTTATTGCGGATTAGttttttctt                                                                                                                                <  2:1840780/150‑5 (MQ=38)
                  aaaCGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTTCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGcgcg                                                                                                                               >  2:296672/1‑150 (MQ=255)
                    aCGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCATTCTTATTGCGGATTAGttttttcttagc                                                                                                                             <  1:637955/150‑8 (MQ=38)
                     cGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTaa                                                                                                                            <  2:470309/150‑1 (MQ=255)
                       gAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAAt                                                                                                                          >  1:1351551/1‑150 (MQ=255)
                        aaaTTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCTGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATg                                                                                                                         >  1:151408/1‑150 (MQ=255)
                         aaTTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCTCAATAAGAATTCGGATTAGTTTTTTCGCGTAAATGt                                                                                                                        >  2:1014588/1‑150 (MQ=255)
                                tttCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATg                                                                                                                 <  2:1417129/150‑1 (MQ=255)
                                  tCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTa                                                                                                               >  2:1626643/1‑150 (MQ=255)
                                  tCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTa                                                                                                               <  2:31926/150‑1 (MQ=255)
                                   cTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTaa                                                                                                              <  2:839278/150‑1 (MQ=255)
                                    tGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAat                                                                                                             <  2:659656/150‑1 (MQ=255)
                                     gATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAata                                                                                                            <  2:457176/150‑1 (MQ=255)
                                      aTGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTAGGAAAATTGTGCTATTAGCTAAGAAAAAAATAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAatat                                                                                                           >  2:214281/1‑150 (MQ=255)
                                       tgtaaaCTTAATCTACATGGCAAAAATTCTTATTTAATACATCAATAATGCCAAAAATAAGTATTAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTc                                                                                                         <  1:804802/147‑1 (MQ=255)
                                       tGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATAtt                                                                                                          >  1:633271/1‑150 (MQ=255)
                                         gAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCAGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTcc                                                                                                        >  2:1682128/1‑150 (MQ=255)
                                         gAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACAAACAGAATGCCAAAAAATAGTATGAAAATTTTGATATTAGATAAGAAAAAACTAATCCTCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTTCAATGTAATATTcc                                                                                                        >  2:192301/1‑150 (MQ=255)
                                          aaaCTTTATCGACCTGGCACATAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGAGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCg                                                                                                       <  2:233109/150‑1 (MQ=255)
                                          aaaCTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCg                                                                                                       <  2:1046307/150‑1 (MQ=255)
                                             cTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATCCCGTaa                                                                                                    >  1:1101471/1‑150 (MQ=255)
                                              tttATCGACCTGGCACAAAATCTTCTTTCAGACATCCATAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAAc                                                                                                   <  1:1900528/150‑1 (MQ=255)
                                                tATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAg                                                                                                 <  1:113602/150‑1 (MQ=255)
                                                 aTCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAgg                                                                                                <  2:1561074/150‑1 (MQ=255)
                                                  tCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGga                                                                                               <  1:1205574/150‑1 (MQ=255)
                                                         ggCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGt                                                                                        <  2:1360296/150‑1 (MQ=255)
                                                           aacaAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTa                                                                                      <  1:1431284/149‑1 (MQ=255)
                                                              aaaaTCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATgg                                                                                   >  1:109872/1‑150 (MQ=255)
                                                               aaaTCTTCTTTCAGACATCCAGAATGCCAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCg                                                                                 <  1:180426/150‑1 (MQ=255)
                                                                 aTCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCgg                                                                                <  1:2046559/150‑1 (MQ=255)
                                                                      cttTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTg                                                                           <  2:381760/150‑1 (MQ=255)
                                                                       tttCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGc                                                                          <  1:531624/150‑1 (MQ=255)
                                                                       tatCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGc                                                                          <  1:1772665/148‑1 (MQ=255)
                                                                        ttCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCa                                                                         <  1:1320136/150‑1 (MQ=255)
                                                                        ttCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCa                                                                         <  1:1909522/150‑1 (MQ=255)
                                                                        ttCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCa                                                                         <  2:1566239/150‑1 (MQ=255)
                                                                           aGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAAt                                                                      >  1:416134/1‑150 (MQ=255)
                                                                            gaaaTCCAGAATGCCAAAAAATAGTATGAAAATTGTTATATTAGCTAAGAAAAAACTAATAAGCAATAAGAATGCGTATTAGTTTTTGCGCGTAAATGTTTCAATGTAATATTCCGTAACAGGATTATCGTTATGGCGTGGTTGAAAATa                                                                     >  1:1239916/4‑150 (MQ=255)
                                                                            gACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATa                                                                     <  1:746050/150‑1 (MQ=255)
                                                                            gACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATa                                                                     >  2:622356/1‑150 (MQ=255)
                                                                            gACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATa                                                                     >  2:809049/1‑150 (MQ=255)
                                                                              cATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGa                                                                   >  1:920760/1‑150 (MQ=255)
                                                                              cATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGa                                                                   >  1:928743/1‑150 (MQ=255)
                                                                               aTCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGAt                                                                  <  1:437105/150‑1 (MQ=255)
                                                                                tCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGAtt                                                                 <  1:1337049/150‑1 (MQ=255)
                                                                                 ccAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGTCGGGGTTGCAAATAGAttt                                                                >  2:1080803/1‑149 (MQ=255)
                                                                                 ccAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTg                                                                >  2:1080798/1‑150 (MQ=255)
                                                                                       tGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGAc                                                          <  1:1000002/150‑1 (MQ=255)
                                                                                        gCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACt                                                         <  2:1629019/150‑1 (MQ=255)
                                                                                         ccAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACtt                                                        >  1:705175/1‑150 (MQ=255)
                                                                                           aaaaaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGAGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaa                                                      >  1:1698573/1‑150 (MQ=255)
                                                                                            aaaaaTAGTATTAAAATTGTTCTATTAGCTAAGAAAAAACTAATCCGCAATAATAATGCGGATTAGTTTTTGCTCGTAAATGTTGCAATGTAATATTCCTTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaac                                                     <  2:1239915/150‑1 (MQ=255)
                                                                                            aaaaaTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaac                                                     >  1:871023/1‑150 (MQ=255)
                                                                                                aTAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTaacaaac                                                 >  2:1631047/1‑150 (MQ=255)
                                                                                                 tAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGATTGACTTaacaaaca                                                >  1:1101618/1‑150 (MQ=255)
                                                                                                     aTGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGAGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGAGGGGTTGCAAATAGATTGCTTGACTTAACAAACAtata                                            >  1:410205/1‑150 (MQ=255)
                                                                                                        aaaaTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACt                                         >  1:152795/1‑150 (MQ=255)
                                                                                                        aaaaTTGTGCTAGTATCTAAGAAAAAACTAATCCGCAATACGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACt                                         <  1:171933/150‑1 (MQ=255)
                                                                                                          aaTTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTg                                       <  2:1531623/150‑1 (MQ=255)
                                                                                                          aaTTGTGATATTAGCTAAGAAAAAACTAATCCGCAATATGAATGCTGATTAGTTTTTGCGAGTTAATGTTGAAATGTAATATTCCGTAACAGGATTATCGTTATGGCGGGGGTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTg                                       <  1:1787546/150‑1 (MQ=255)
                                                                                                            ttGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAACGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGAc                                     <  1:341665/150‑1 (MQ=255)
                                                                                                             tgtgCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATTCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACa                                    <  2:1063356/150‑1 (MQ=255)
                                                                                                                 cTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGaaa                                >  1:1187601/1‑150 (MQ=255)
                                                                                                                  tATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAAAAGATAACTTGACAGaaaa                               >  1:1527831/1‑150 (MQ=255)
                                                                                                                      aGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATa                           >  1:1704176/1‑150 (MQ=255)
                                                                                                                        cTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaa                         >  2:1418386/1‑150 (MQ=255)
                                                                                                                          gcgcaaaaaCTAATCCGCATTCTTATTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaaaa                       >  1:751515/5‑150 (MQ=38)
                                                                                                                            gAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATaaaaaaa                     >  2:426645/1‑150 (MQ=255)
                                                                                                                             caaaaaCTAATCCGCATTCTTATTGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATATAAAAag                    >  1:222592/2‑150 (MQ=39)
                                                                                                                               aaaaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAagag                  <  1:1626644/150‑1 (MQ=255)
                                                                                                                                 aaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGcc                <  2:1639878/150‑1 (MQ=255)
                                                                                                                                 aaCTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGcc                <  2:1010849/150‑1 (MQ=255)
                                                                                                                                   cTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGGTCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAg              >  2:497045/1‑150 (MQ=255)
                                                                                                                                     aaTCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCg            >  1:1000223/1‑150 (MQ=255)
                                                                                                                                       tCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATGACTTGACAGAAAAGATAAAAAAAGAGCCAGCggg          >  1:531130/1‑150 (MQ=255)
                                                                                                                                       tCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGAAATGTAAAATTACGTAACAGGATGATCGTGATGGCGGGGTTGCAACTAGCTTGCTTGCCTTAACAAACAGATAACTTGACAGCACAGATAAAAAAAGAGCCAgatgg          >  2:1080982/1‑146 (MQ=255)
                                                                                                                                        ccGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgc         >  2:1558515/1‑150 (MQ=255)
                                                                                                                                         cGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGgcg        >  2:215971/1‑150 (MQ=255)
                                                                                                                                            aaTAAGAATGCGGTTTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTg     <  1:1991745/150‑1 (MQ=255)
                                                                                                                                              tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGc   <  2:1351550/150‑1 (MQ=255)
                                                                                                                                              tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGc   >  2:864271/1‑150 (MQ=255)
                                                                                                                                              tAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCAGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGc   <  1:1163664/150‑1 (MQ=255)
                                                                                                                                               aaGAATGCTGATTAGTTTTTGCGCGTAAATGTTGCAATTTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGct  <  1:177551/150‑1 (MQ=255)
                                                                                                                                               aaGAATGCGGATTAGTTTTTGCGCGTAAATGTTTCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGct  <  1:275676/150‑1 (MQ=255)
                                                                                                                                               aaGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTACCAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGct  <  1:296672/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
CTTATATTTAAATTAACTAAACGGAAATTTTGTTTCTGATGGAAACTTTATCGACCTGGCACAAAATCTTCTTTCAGACATCCAGAATGCCAAAAAATAGTATGAAAATTGTGCTATTAGCTAAGAAAAAACTAATCCGCAATAAGAATGCGGATTAGTTTTTGCGCGTAAATGTTGCAATGTAATATTCCGTAACAGGATGATCGTTATGGCGGGGTTGCAAATAGATTGCTTGACTTAACAAACAGATAACTTGACAGAAAAGATAAAAAAAGAGCCAGCGGGCGCTGGCT  >  NC_000913/987277‑987569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: