Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,026,539 T→A 5.8% intergenic (+14/+18) helD → / ← mgsA DNA helicase IV/methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,026,5390TA 5.8% 338.1 / 7.2 118intergenic (+14/+18)helD/mgsADNA helicase IV/methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base T (64/46);  new base A (2/5);  total (66/52)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 123 total aligned reads displayed.

TGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCGT  >  NC_000913/1026390‑1026688
                                                                                                                                                     |                                                                                                                                                     
tGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGt                                                                                                                                                       >  1:1441269/1‑150 (MQ=255)
tGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGt                                                                                                                                                       >  1:1628301/1‑150 (MQ=255)
 gaagaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGtt                                                                                                                                                      >  1:1822709/1‑150 (MQ=255)
  aagaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGttt                                                                                                                                                     >  2:1499362/1‑150 (MQ=255)
    gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGGTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                   <  2:1084570/150‑1 (MQ=255)
      aCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTAGGGGCCCTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCa                                                                                                                                                 <  2:1429525/150‑1 (MQ=255)
        ggcggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCAcc                                                                                                                                               <  2:1558711/150‑1 (MQ=255)
           ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGCATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCCCCtgt                                                                                                                                            >  2:1355753/1‑150 (MQ=255)
           ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAATTACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgt                                                                                                                                            <  2:1395069/150‑1 (MQ=255)
            gTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCtgtg                                                                                                                                           <  2:522240/150‑1 (MQ=255)
               aaTGTACGTGGCTCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAAAACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttct                                                                                                                                        <  1:447241/150‑5 (MQ=255)
                aTGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttctt                                                                                                                                       <  1:1467502/150‑6 (MQ=255)
                  gTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttcttac                                                                                                                                     <  2:509094/150‑8 (MQ=255)
                   tatgTGGCGATGACCCGGTCACGCCAGCGGGTATTGGCACTGTTTAACAAAGAGTATCCCTCTCCCTTTGTGGATATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATAtt                                                                                                                                    <  2:1782493/147‑1 (MQ=255)
                   tACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATAtt                                                                                                                                    <  2:1480422/150‑1 (MQ=255)
                   tACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTATCCCTTTGTGGAATTACTGAAAAATCTTGATGTGCCGGTGGCGAGAAAACCGTAAGAAACATGTGGAGTTTGCCACCTGTGCAATAtt                                                                                                                                    >  1:872470/1‑150 (MQ=255)
                       tGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACtt                                                                                                                                <  1:659987/150‑1 (MQ=255)
                       tGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACtt                                                                                                                                >  2:1286994/1‑150 (MQ=255)
                         gcgcTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCa                                                                                                                              <  2:1816297/150‑1 (MQ=255)
                         gcgcTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCa                                                                                                                              <  1:493471/150‑1 (MQ=255)
                              gACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACgg                                                                                                                         >  2:313634/1‑150 (MQ=255)
                                cccGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTc                                                                                                                       >  2:442657/1‑150 (MQ=255)
                                   gggCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCcgc                                                                                                                    >  1:1472259/1‑150 (MQ=255)
                                      cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGTATGCCTCTCCCTTTGTGGAAATTCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 <  1:157260/150‑1 (MQ=255)
                                      cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAGAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 >  2:114397/1‑150 (MQ=255)
                                      cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 <  1:1711257/150‑1 (MQ=255)
                                        cGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGAt                                                                                                               >  1:1630486/1‑150 (MQ=255)
                                         gCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATa                                                                                                              >  1:640743/1‑150 (MQ=255)
                                          ccATCGGGTATGGACACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATaa                                                                                                             >  1:1452447/1‑150 (MQ=255)
                                           cATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAAc                                                                                                            >  1:1547496/1‑150 (MQ=255)
                                                      ggCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCg                                                                                                 <  1:1147683/150‑1 (MQ=255)
                                                        cACTGTTTAACATAGACACTCCGTCTCCCTTTGTGGAAAAACTGAAAAATCTGGATGTGCCGGTGGCGCGAAAACCGTAAGAAACCGGGGGCGTTTGCCACCGGTGCAATATTACTTCAGCCGGTCCGCGAGATTACGCTGATAATgggg                                                                                               <  2:104949/150‑1 (MQ=255)
                                                         acggTTTAACAAAGAGAATCCCTCTCCCTTTTTGGAAATACTGAATAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGGCCGCGAGATAACGCTGATAATCgggg                                                                                              <  1:136805/147‑1 (MQ=255)
                                                          cTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGGTAACGCTGATAATCGGGGa                                                                                             <  1:758152/150‑1 (MQ=255)
                                                          cTGTTTAACAAAGAGAATCCCTCTACCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGa                                                                                             <  1:371618/150‑1 (MQ=255)
                                                           tGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGAt                                                                                            <  1:600620/150‑1 (MQ=255)
                                                              ttAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAg                                                                                         <  2:1408846/150‑1 (MQ=255)
                                                                aaCAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGaa                                                                                       <  2:2032141/150‑1 (MQ=255)
                                                                aaCAAAGAGAATCCCGCTCCCTTTGTGGAAATACTGAAAAATCGGGATGTGCCGGGGGCGAGAACACCGTATGAAACAGGTGGCGTTTGCCACCTGGGCGATATTACTGCCGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGaa                                                                                       >  2:530182/1‑150 (MQ=255)
                                                                  cAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGATGCTAATCGGGGATCAGAata                                                                                     >  2:1793444/1‑150 (MQ=255)
                                                                   aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAatat                                                                                    >  1:1816902/1‑150 (MQ=255)
                                                                   aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAatat                                                                                    >  2:501754/1‑150 (MQ=255)
                                                                    aaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATc                                                                                   >  1:1881872/1‑150 (MQ=255)
                                                                        gaATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGAcc                                                                               >  2:1370500/1‑150 (MQ=255)
                                                                           tCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTGAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACagcg                                                                            >  1:1051304/1‑150 (MQ=255)
                                                                           acccTCTCCCTTTGTGGAAATAATGAAAAATCTGGCTGTGCCGGTGGCGAGAAAACCGGAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACcgcg                                                                            <  2:402821/149‑1 (MQ=255)
                                                                             ccTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtc                                                                          >  1:222807/1‑150 (MQ=255)
                                                                              ctctCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtcg                                                                         >  2:1345177/1‑150 (MQ=255)
                                                                                 tcCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTg                                                                      >  1:1100629/1‑150 (MQ=255)
                                                                                 tcCCTTTGTGCAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAACACCGTAAGAAACCGGTGGCGGTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGTTAACGCTGATAATCGGGGATCAGAATATCGACCGCGGCGTTg                                                                      <  1:1026603/150‑1 (MQ=255)
                                                                                  cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATATCGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  1:1470654/1‑150 (MQ=255)
                                                                                  cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  2:683577/1‑150 (MQ=255)
                                                                                  cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCGGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  2:1721780/1‑150 (MQ=255)
                                                                                   ccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGaa                                                                    >  1:495859/1‑150 (MQ=255)
                                                                                        gtgGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATgcg                                                               >  2:167681/1‑150 (MQ=255)
                                                                                         tgGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGTATCAGAATATCGACCGCGTCGTTGAAATgcgg                                                              >  2:1120073/1‑150 (MQ=255)
                                                                                         tgGAAATACTCAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGAGCAATATTACTACAGACGGTCCGCGAGATAACGCTGATAAACGGGGATCAGAATATCGACCGCGTCGTAGAAATccgg                                                              >  2:500092/1‑150 (MQ=255)
                                                                                          ggAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATgcggc                                                             >  2:682302/1‑150 (MQ=255)
                                                                                                aCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTgg                                                       <  2:1855273/150‑1 (MQ=255)
                                                                                                aCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTgg                                                       >  2:2019729/1‑150 (MQ=255)
                                                                                                 cTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGGAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGa                                                      >  2:653332/1‑150 (MQ=255)
                                                                                                  tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     <  2:694596/150‑1 (MQ=255)
                                                                                                  tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     >  2:1437543/1‑150 (MQ=255)
                                                                                                  tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     <  1:1823058/150‑1 (MQ=255)
                                                                                                  tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     >  2:1889309/1‑150 (MQ=255)
                                                                                                  tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     <  2:1737743/150‑1 (MQ=255)
                                                                                                   gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGCCGGTCCGCGAGATACCGCTGATTAGCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    >  1:973941/1‑150 (MQ=255)
                                                                                                   gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    >  1:1980449/1‑150 (MQ=255)
                                                                                                   gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    <  2:1631011/150‑1 (MQ=255)
                                                                                                   gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    >  1:1365104/1‑150 (MQ=255)
                                                                                                     aaaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTAt                                                  >  1:1084772/1‑150 (MQ=255)
                                                                                                      aaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATg                                                 <  1:673615/150‑1 (MQ=255)
                                                                                                       aaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGa                                                <  2:754580/150‑1 (MQ=255)
                                                                                                           tGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGCCGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTc                                            >  2:333065/1‑150 (MQ=255)
                                                                                                             caTGTGCCGGGGGCGAGAAACCCGTAAGAAACAGCGGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGCCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTATGCAGCCTg                                          <  2:1904839/149‑1 (MQ=255)
                                                                                                               tgtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGcc                                        >  2:732403/1‑150 (MQ=255)
                                                                                                               tgtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGcc                                        <  2:1881871/150‑1 (MQ=255)
                                                                                                                gtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCg                                       <  1:210492/150‑1 (MQ=255)
                                                                                                                 tgCCGGTGGCGAGAAAACCGTAAGAAACCGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGGGTCGTTGAAATGCGTCGACTGGATTATGAAGTCTGCAGt                                      >  1:57504/1‑150 (MQ=255)
                                                                                                                  gCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGtt                                     >  2:944126/1‑150 (MQ=255)
                                                                                                                  gCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGtt                                     >  1:89480/1‑150 (MQ=255)
                                                                                                                        ggCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCCCg                               >  2:1521698/1‑150 (MQ=255)
                                                                                                                         gCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGt                              <  1:1508020/150‑1 (MQ=255)
                                                                                                                                   cGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAc                    >  2:950905/1‑150 (MQ=255)
                                                                                                                                    gTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                   >  1:1572555/1‑150 (MQ=255)
                                                                                                                                    gTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                   >  1:474703/1‑150 (MQ=255)
                                                                                                                                     tAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCg                  <  1:1277200/150‑1 (MQ=255)
                                                                                                                                        gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGaa               >  2:2028915/1‑150 (MQ=255)
                                                                                                                                        gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGaa               <  1:831355/150‑1 (MQ=255)
                                                                                                                                        gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGaa               >  1:1850508/1‑150 (MQ=255)
                                                                                                                                           aCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGt            >  1:329067/1‑150 (MQ=255)
                                                                                                                                             aGGTGTCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTc          <  1:566747/150‑1 (MQ=255)
                                                                                                                                              gggggCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGATCGGAATGTTcc         <  2:1922398/147‑1 (MQ=255)
                                                                                                                                              ggTGGCGTTTGCCACCTGTGCAATATTACTTCAGCCGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGTCCGGAATGTTcc         >  1:1817330/1‑150 (MQ=255)
                                                                                                                                              ggTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTcc         <  2:1822708/150‑1 (MQ=255)
                                                                                                                                               ggggCGATTGCCACCTGTGCAATATTACTTCAGACGGGCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCa        <  1:384404/148‑1 (MQ=255)
                                                                                                                                                 ggCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATa      >  2:2015853/1‑150 (MQ=255)
                                                                                                                                                   cGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGAGTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGCCCGGAATGTTCCATAcc    >  2:150978/1‑150 (MQ=255)
                                                                                                                                                    gTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCg   <  1:1364418/150‑1 (MQ=255)
                                                                                                                                                     tttGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGTGACCGGAATGTTCCATACCGt  <  1:1785666/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCGT  >  NC_000913/1026390‑1026688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: