Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,026,540 T→A 5.0% intergenic (+15/+17) helD → / ← mgsA DNA helicase IV/methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,026,5400TA 5.0% 348.0 / 5.0 118intergenic (+15/+17)helD/mgsADNA helicase IV/methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base T (64/48);  new base A (2/4);  total (66/52)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 121 total aligned reads displayed.

GAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCGT  >  NC_000913/1026391‑1026688
                                                                                                                                                     |                                                                                                                                                    
gaagaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGtt                                                                                                                                                      >  1:1822709/1‑150 (MQ=255)
   gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGGTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                   <  2:1084570/150‑1 (MQ=255)
     aCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCa                                                                                                                                                 >  2:1982022/1‑150 (MQ=255)
     aCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTAGGGGCCCTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCa                                                                                                                                                 <  2:1429525/150‑1 (MQ=255)
       ggcggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCAcc                                                                                                                                               <  2:1558711/150‑1 (MQ=255)
          ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGCATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCCCCtgt                                                                                                                                            >  2:1355753/1‑150 (MQ=255)
          ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAATTACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgt                                                                                                                                            <  2:1395069/150‑1 (MQ=255)
           gTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCtgtg                                                                                                                                           <  2:522240/150‑1 (MQ=255)
            ttAATGTACGTGGCGCTGACCCTGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGc                                                                                                                                          <  2:1151249/150‑1 (MQ=255)
              aaTGTACGTGGCTCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAAAACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttct                                                                                                                                        <  1:447241/150‑5 (MQ=255)
               aTGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttctt                                                                                                                                       <  1:1467502/150‑6 (MQ=255)
                 gTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttcttac                                                                                                                                     <  2:509094/150‑8 (MQ=255)
                  tatgTGGCGATGACCCGGTCACGCCAGCGGGTATTGGCACTGTTTAACAAAGAGTATCCCTCTCCCTTTGTGGATATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATAtt                                                                                                                                    <  2:1782493/147‑1 (MQ=255)
                  tACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATAtt                                                                                                                                    <  2:1480422/150‑1 (MQ=255)
                  tACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTATCCCTTTGTGGAATTACTGAAAAATCTTGATGTGCCGGTGGCGAGAAAACCGTAAGAAACATGTGGAGTTTGCCACCTGTGCAATAtt                                                                                                                                    >  1:872470/1‑150 (MQ=255)
                     gTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACt                                                                                                                                 <  2:1776394/150‑1 (MQ=255)
                      tGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACtt                                                                                                                                <  1:659987/150‑1 (MQ=255)
                        gcgcTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCa                                                                                                                              <  1:493471/150‑1 (MQ=255)
                             gACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACgg                                                                                                                         >  2:313634/1‑150 (MQ=255)
                                  gggCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCcgc                                                                                                                    >  1:1472259/1‑150 (MQ=255)
                                    gCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCga                                                                                                                  <  2:1392813/150‑1 (MQ=255)
                                     cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGTATGCCTCTCCCTTTGTGGAAATTCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 <  1:157260/150‑1 (MQ=255)
                                     cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAGAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 >  2:114397/1‑150 (MQ=255)
                                     cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 <  1:1711257/150‑1 (MQ=255)
                                     cACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCgag                                                                                                                 <  2:1441268/150‑1 (MQ=255)
                                       cGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGAt                                                                                                               >  1:1630486/1‑150 (MQ=255)
                                        gCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATa                                                                                                              >  1:640743/1‑150 (MQ=255)
                                             cGGGTATGGGCACTGTTTAACAAAGAGACTCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGACCGCGAGATAACGCt                                                                                                         <  1:1853990/150‑1 (MQ=255)
                                                     ggCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCg                                                                                                 <  1:1147683/150‑1 (MQ=255)
                                                       cACTGTTTAACATAGACACTCCGTCTCCCTTTGTGGAAAAACTGAAAAATCTGGATGTGCCGGTGGCGCGAAAACCGTAAGAAACCGGGGGCGTTTGCCACCGGTGCAATATTACTTCAGCCGGTCCGCGAGATTACGCTGATAATgggg                                                                                               <  2:104949/150‑1 (MQ=255)
                                                        acggTTTAACAAAGAGAATCCCTCTCCCTTTTTGGAAATACTGAATAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGGCCGCGAGATAACGCTGATAATCgggg                                                                                              <  1:136805/147‑1 (MQ=255)
                                                         cTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGGTAACGCTGATAATCGGGGa                                                                                             <  1:758152/150‑1 (MQ=255)
                                                         cTGTTTAACAAAGAGAATCCCTCTACCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGa                                                                                             <  1:371618/150‑1 (MQ=255)
                                                          tGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGAt                                                                                            <  1:600620/150‑1 (MQ=255)
                                                               aaCAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGaa                                                                                       <  1:1812953/150‑1 (MQ=255)
                                                               aaCAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGaa                                                                                       <  2:2032141/150‑1 (MQ=255)
                                                                 cAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAata                                                                                     >  1:970931/1‑150 (MQ=255)
                                                                 cAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGATGCTAATCGGGGATCAGAata                                                                                     >  2:1793444/1‑150 (MQ=255)
                                                                  aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAatat                                                                                    >  2:501754/1‑150 (MQ=255)
                                                                  aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAatat                                                                                    >  1:1816902/1‑150 (MQ=255)
                                                                   aaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATc                                                                                   >  1:1881872/1‑150 (MQ=255)
                                                                       gaATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGAcc                                                                               >  2:1370500/1‑150 (MQ=255)
                                                                          tCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTGAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACagcg                                                                            >  1:1051304/1‑150 (MQ=255)
                                                                          acccTCTCCCTTTGTGGAAATAATGAAAAATCTGGCTGTGCCGGTGGCGAGAAAACCGGAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACcgcg                                                                            <  2:402821/149‑1 (MQ=255)
                                                                           cccTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGAGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgt                                                                           >  1:150772/1‑150 (MQ=255)
                                                                             ctctCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtcg                                                                         >  2:1345177/1‑150 (MQ=255)
                                                                                tcCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTg                                                                      >  1:1100629/1‑150 (MQ=255)
                                                                                tcCCTTTGTGCAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAACACCGTAAGAAACCGGTGGCGGTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGTTAACGCTGATAATCGGGGATCAGAATATCGACCGCGGCGTTg                                                                      <  1:1026603/150‑1 (MQ=255)
                                                                                 cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATATCGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  1:1470654/1‑150 (MQ=255)
                                                                                 cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  2:1835186/1‑150 (MQ=255)
                                                                                 cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  2:683577/1‑150 (MQ=255)
                                                                                 cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCGGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                     >  2:1721780/1‑150 (MQ=255)
                                                                                  ccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGaa                                                                    >  1:495859/1‑150 (MQ=255)
                                                                                        tgGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGTATCAGAATATCGACCGCGTCGTTGAAATgcgg                                                              >  2:1120073/1‑150 (MQ=255)
                                                                                        tgGAAATACTCAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGAGCAATATTACTACAGACGGTCCGCGAGATAACGCTGATAAACGGGGATCAGAATATCGACCGCGTCGTAGAAATccgg                                                              >  2:500092/1‑150 (MQ=255)
                                                                                         ggAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATgcggc                                                             >  2:682302/1‑150 (MQ=255)
                                                                                               aCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTgg                                                       >  2:2019729/1‑150 (MQ=255)
                                                                                               aCTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTgg                                                       <  2:1855273/150‑1 (MQ=255)
                                                                                                cTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGGAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGa                                                      >  2:653332/1‑150 (MQ=255)
                                                                                                cTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGa                                                      >  1:1377981/1‑150 (MQ=255)
                                                                                                 tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     >  2:1437543/1‑150 (MQ=255)
                                                                                                 tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     >  2:1889309/1‑150 (MQ=255)
                                                                                                 tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     <  2:694596/150‑1 (MQ=255)
                                                                                                 tGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAt                                                     <  2:1737743/150‑1 (MQ=255)
                                                                                                  gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    >  1:1603518/1‑150 (MQ=255)
                                                                                                  gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    >  1:1365104/1‑150 (MQ=255)
                                                                                                  gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    <  2:1631011/150‑1 (MQ=255)
                                                                                                  gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                    >  1:1980449/1‑150 (MQ=255)
                                                                                                  gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAAAGCGGCGACTGGAtt                                                    >  2:48971/1‑150 (MQ=255)
                                                                                                    aaaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTAt                                                  >  1:1084772/1‑150 (MQ=255)
                                                                                                     aaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATg                                                 <  1:673615/150‑1 (MQ=255)
                                                                                                      aaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGa                                                <  2:754580/150‑1 (MQ=255)
                                                                                                          tGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGCCGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTc                                            >  2:333065/1‑150 (MQ=255)
                                                                                                              tgtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGcc                                        >  2:732403/1‑150 (MQ=255)
                                                                                                              tgtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGcc                                        <  2:1881871/150‑1 (MQ=255)
                                                                                                               gtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCg                                       <  1:210492/150‑1 (MQ=255)
                                                                                                                tgCCGGTGGCGAGAAAACCGTAAGAAACCGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGGGTCGTTGAAATGCGTCGACTGGATTATGAAGTCTGCAGt                                      >  1:57504/1‑150 (MQ=255)
                                                                                                                 gCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGtt                                     >  1:89480/1‑150 (MQ=255)
                                                                                                                 gCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGtt                                     >  2:944126/1‑150 (MQ=255)
                                                                                                                       ggCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCCCg                               >  2:1521698/1‑150 (MQ=255)
                                                                                                                        gCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGt                              <  1:1508020/150‑1 (MQ=255)
                                                                                                                                  cGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAc                    >  2:950905/1‑150 (MQ=255)
                                                                                                                                   gTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                   >  1:1572555/1‑150 (MQ=255)
                                                                                                                                   gTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAcc                   >  1:474703/1‑150 (MQ=255)
                                                                                                                                    tAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCg                  >  2:270551/1‑150 (MQ=255)
                                                                                                                                    tAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCg                  <  1:1277200/150‑1 (MQ=255)
                                                                                                                                       gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGaa               <  1:831355/150‑1 (MQ=255)
                                                                                                                                       gAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGaa               >  1:1850508/1‑150 (MQ=255)
                                                                                                                                         caCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATg             >  2:648919/2‑150 (MQ=255)
                                                                                                                                          aCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGt            >  1:329067/1‑150 (MQ=255)
                                                                                                                                            aGGTGTCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTc          <  1:566747/150‑1 (MQ=255)
                                                                                                                                            aGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTc          <  2:1628300/150‑1 (MQ=255)
                                                                                                                                             gggggCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGATCGGAATGTTcc         <  2:1922398/147‑1 (MQ=255)
                                                                                                                                             ggTGGCGTTTGCCACCTGTGCAATATTACTTCAGCCGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGTCCGGAATGTTcc         >  1:1817330/1‑150 (MQ=255)
                                                                                                                                             ggTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTcc         <  2:1822708/150‑1 (MQ=255)
                                                                                                                                              ggggCGATTGCCACCTGTGCAATATTACTTCAGACGGGCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCa        <  1:384404/148‑1 (MQ=255)
                                                                                                                                                ggCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATa      >  2:2015853/1‑150 (MQ=255)
                                                                                                                                                ggCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTACCACGTTGGTGGCGACCGGAATGTTCCATa      <  1:313634/150‑1 (MQ=255)
                                                                                                                                                  cGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGAGTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGCCCGGAATGTTCCATAcc    >  2:150978/1‑150 (MQ=255)
                                                                                                                                                   gTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCg   <  1:1364418/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACCGT  >  NC_000913/1026391‑1026688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: