Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,063,264 C→A 9.9% G171V (GGT→GTT)  cbpA ← DnaK co‑chaperone; curved DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,063,2640CA 9.9% 292.8 / 6.1 119G171V (GGT→GTT) cbpADnaK co‑chaperone; curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (55/50);  new base A (2/12);  total (57/62)
Fisher's exact test for biased strand distribution p-value = 9.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATT  >  NC_000913/1063116‑1063413
                                                                                                                                                    |                                                                                                                                                     
ccACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGAcc                                                                                                                                                      >  2:427293/1‑150 (MQ=255)
ccACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGAcc                                                                                                                                                      >  1:628485/1‑150 (MQ=255)
    gggCTAACCGGCACCAGAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCTCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTg                                                                                                                                                  <  1:17650/150‑1 (MQ=255)
     ggCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCCTCCCCTGGCCTTTCAGACGGATCCGTTGACCATTGc                                                                                                                                                 >  2:1238663/1‑150 (MQ=255)
       cTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCg                                                                                                                                               >  2:1179125/1‑150 (MQ=255)
       cTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCg                                                                                                                                               >  1:543461/1‑150 (MQ=255)
         aaCCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGAc                                                                                                                                             >  1:1945625/1‑150 (MQ=255)
          aCCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGccgccg                                                                                                                                            >  1:1230792/1‑150 (MQ=255)
          aCCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGAcg                                                                                                                                            <  2:1384471/150‑1 (MQ=255)
          aCCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGAcg                                                                                                                                            >  2:1430400/1‑150 (MQ=255)
             ggCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGAcgccc                                                                                                                                         <  2:568521/150‑1 (MQ=255)
               caccacAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGAcgcccgc                                                                                                                                       >  2:1479484/1‑150 (MQ=255)
                accacAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACCAATCGCCATTTGGACAGCCGTTTTCGCCCGGCGTCCCCTGGCCATTCAGCCGGATGCGTTGCCCCTTGCCGAcgcccgac                                                                                                                                      >  1:309288/1‑148 (MQ=255)
                 ccacAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCAGTTGGACAGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGCCGGATGCGATGAACATTGCAGACGCccgccg                                                                                                                                     >  2:135948/1‑150 (MQ=255)
                  cacaATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCgg                                                                                                                                    >  1:82927/1‑150 (MQ=255)
                   acaATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCggg                                                                                                                                   >  2:884822/1‑150 (MQ=255)
                   acaATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCggg                                                                                                                                   <  2:962698/150‑1 (MQ=255)
                   acaATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCggg                                                                                                                                   >  1:445486/1‑150 (MQ=255)
                   acaATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCggg                                                                                                                                   <  2:1020200/150‑1 (MQ=255)
                       ttgccAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCCCAAATCGGCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCATTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCt                                                                                                                               <  2:977209/147‑1 (MQ=255)
                        ttCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCtt                                                                                                                              >  1:1933062/1‑150 (MQ=255)
                            aGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTcaca                                                                                                                          >  1:1580472/1‑150 (MQ=255)
                               tCCTGGGCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGGCGTTTTCGCCCGGCGTCCCCTGGCC‑TTCAGACGGATGCGTTGTCCATTGCCGACGCCCGCCGTGATCTTCACATTCa                                                                                                                      <  2:510356/150‑1 (MQ=255)
                               tCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTc                                                                                                                       <  1:1428434/150‑1 (MQ=255)
                                       gACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGTATTTGTTTTAATTTTACGTCGACCTCCGTGATCTTCACATTCAGCGtttt                                                                                                               <  2:924799/150‑1 (MQ=255)
                                        aCAATATCAAACCGCGGAGGTGGCGCAATATGAATCACCAGCCACCAATCGCCATTTGGACCGCCGTTTTCGCCCGTCGTCCCCTGCCCTTTCTGTCGGTTGTGTTTAAATTTTACGTCGACCTCCGTGATCTTCACATTCAGCGTTTTc                                                                                                              <  2:305030/150‑1 (MQ=255)
                                        aCAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTc                                                                                                              <  1:995419/150‑1 (MQ=255)
                                         cAATATCAAACAGCGGATGTGGCGCAATATGAATCCCCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGACGGGATCGTCACATTCAGCGTTTTCg                                                                                                             >  1:826560/1‑150 (MQ=255)
                                         cAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCg                                                                                                             <  2:490508/150‑1 (MQ=255)
                                             atCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAAt                                                                                                         >  1:1944523/1‑150 (MQ=255)
                                             atCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAAt                                                                                                         >  1:561944/1‑150 (MQ=255)
                                              tCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATATTCACATTCTGCGTTTTAGGAAtt                                                                                                        >  1:1779307/1‑150 (MQ=255)
                                              tCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCAGGCGTCCCCTGGCCTTTCAGACGGATGCATTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAAtt                                                                                                        >  1:1169067/1‑150 (MQ=255)
                                               cAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAAttt                                                                                                       >  2:209661/1‑150 (MQ=255)
                                                aaaCAGCGGCTGGGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTGCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTGGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTc                                                                                                      <  1:818253/150‑1 (MQ=255)
                                                aaaCAGCGGATGTGGCTCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCTCCCGGCGACCCCTACCCATTCAGACCGATGCGTTGACCATTGCCGACGCACGCCAAGATATTAAAATACAGCGTTTTAGGAAttta                                                                                                      >  2:569681/1‑149 (MQ=255)
                                                aaaCACCGCATGTGGCTCAAAATGAATCACCAGCCACAACTCGCCATTTGGACCGCCTTTTTCGCCCGGCGTCCCCTGGCCATTCAGACTTATTTTTTTTAAATTTACGTCGACCTCCGTGATCTTCACATTCAGCGTTTTCGGAATTTc                                                                                                      <  2:354690/150‑1 (MQ=255)
                                                 aaCAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGTGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTcc                                                                                                     >  2:1956933/1‑150 (MQ=255)
                                                 aaCAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTcc                                                                                                     <  1:728842/150‑1 (MQ=255)
                                                 aaCAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTcc                                                                                                     <  2:1091334/150‑1 (MQ=255)
                                                 aaCAGCGGATGTGGCGCAATATGAAGCACCATCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGAGCTTCACATTCAGCGTTTTCGGAATTTcc                                                                                                     <  1:990788/150‑1 (MQ=255)
                                                     gCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGtt                                                                                                 <  1:1167166/150‑1 (MQ=255)
                                                          tgtgGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCATGTTCGATc                                                                                            >  2:1384856/1‑150 (MQ=255)
                                                           gtgGCGCAATATGAATCACCAGCCAAAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCGCCTGGCCTATCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCa                                                                                           <  1:972361/150‑1 (MQ=255)
                                                            tgGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTGTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTGTTCGGAATTTCCTGTTCGATCAt                                                                                          >  1:1786790/1‑150 (MQ=255)
                                                               cgcAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGcc                                                                                       >  2:196222/1‑150 (MQ=255)
                                                                    tatGAATCACCAGAAACAAATCGCCATTTGGACCGCCTTTTACGCCCGGCGTCCCCTGGCCTTTCAGACTTTTTTGTTTAAATTTTACGTCGACCGCCGTGATCTTCACATTCAGAGTTTTCGGAATTTCCTGTTCGATCATGGCAAAgg                                                                                  <  2:298202/150‑1 (MQ=255)
                                                                      tGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACTTATGTGTTGAAAATTGCCGACGACCGCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCg                                                                                <  2:1121014/150‑1 (MQ=255)
                                                                         aTCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATTCTTTTTACTTTGACGTCGACCGCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTta                                                                             <  2:1023587/150‑1 (MQ=255)
                                                                         aTCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTta                                                                             <  1:368319/150‑1 (MQ=255)
                                                                         aTCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTAAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTta                                                                             >  2:552764/1‑150 (MQ=255)
                                                                          tCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTtat                                                                            <  1:1746831/150‑1 (MQ=255)
                                                                            aCCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATaa                                                                          >  1:341061/1‑150 (MQ=255)
                                                                                gCCACAAATAGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCg                                                                      >  2:1677687/1‑150 (MQ=255)
                                                                                 ccACAAATCGCCATTTGTAGCGCCGTTTTCTCGCGGCGTCCAGTGGCCTTTCATACGTATGCGTTGACCATTGACTTCGACCGCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCgg                                                                     <  2:95068/150‑1 (MQ=255)
                                                                                 ccACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACTGATTCGTTGACAATTGCCGACGCCCTCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCgg                                                                     <  2:769750/150‑1 (MQ=255)
                                                                                  cacaAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGc                                                                    >  1:760393/1‑150 (MQ=255)
                                                                                  cacaAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGc                                                                    >  1:1961420/1‑150 (MQ=255)
                                                                                   acaAATCGCCATTTTGAACGCCGTTTTCGCGCGGCGTCCCCTGGCCTTTCAGACGGATTTGTTGTACATTTCCGACGCCCTCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCa                                                                   <  2:1290313/150‑1 (MQ=255)
                                                                                   acaAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGTCGTTATAAACAGGCa                                                                   >  2:1301800/1‑150 (MQ=255)
                                                                                   acaAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCa                                                                   <  1:586543/150‑1 (MQ=255)
                                                                                    caAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGCCGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACGTTCAGCGTTTTCGGATTTTCCTGTTCGATCATGCCAACGGCGTTATAAGCCGGCAg                                                                  >  1:1236763/1‑150 (MQ=255)
                                                                                    caAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGTTGTGTTTTAATTTTACGTCGCCCGCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAg                                                                  <  2:156308/150‑1 (MQ=255)
                                                                                     aaaTCGCCATCTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAgg                                                                 <  1:267166/150‑1 (MQ=255)
                                                                                       aTCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGGCGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCTTTATAAACCGGCAGGtt                                                               >  2:271277/1‑150 (MQ=255)
                                                                                         cGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCCGACGGATGCGTTGACCTTTGCCGTCGCCCTCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTtat                                                             <  2:1578759/150‑1 (MQ=255)
                                                                                          gCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTtata                                                            >  1:829609/1‑150 (MQ=255)
                                                                                            cATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGc                                                          <  2:1753659/150‑1 (MQ=255)
                                                                                             aTTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCt                                                         <  1:1266449/150‑1 (MQ=255)
                                                                                                tGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACTGATGTTTTTAAAATTGACGTCGACCTCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGAt                                                      <  2:113196/150‑1 (MQ=255)
                                                                                                 ggACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGCCGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATg                                                     >  1:1367512/1‑150 (MQ=255)
                                                                                                    ccgccgTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCGGAGGGTa                                                  >  1:171516/1‑150 (MQ=255)
                                                                                                      gccgTTTTCGCCCGGCGTCCCCTGGCGTTTCAGCCCGCTGCGTTGACCATTGCCGACGCCCGACGGCATCTTCACATTCCGCGTTTTCGGATGTTCCTGTGCGATCGTGCCAAAGGCGTTATACCCCGGCAGGTTATCGCTGATCGTACg                                                >  2:118974/1‑150 (MQ=255)
                                                                                                      gccgTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGTATTATTTTAAAATTTACGTCGACCTCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACg                                                <  2:1661159/150‑1 (MQ=255)
                                                                                                         gTTCTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCtt                                             <  1:414625/150‑1 (MQ=255)
                                                                                                          tgttCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTa                                            <  1:867265/148‑1 (MQ=255)
                                                                                                            ttCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATg                                          <  1:196222/150‑1 (MQ=255)
                                                                                                              cGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCt                                        <  1:1185279/150‑1 (MQ=255)
                                                                                                                  cGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAACGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGt                                    >  2:1789811/1‑150 (MQ=255)
                                                                                                                   ggCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTa                                   <  1:1018567/150‑1 (MQ=255)
                                                                                                                       tCCCCTGGCCTTTCAGACGGATGTGTTGACCTTTGACGACGCCCTCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCg                               <  2:154270/150‑1 (MQ=255)
                                                                                                                       tCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCTCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCg                               <  2:1230791/150‑1 (MQ=255)
                                                                                                                         cccTGGCCTTTCAGACGGATGCGTTTACCATTGCCGACGCCCGCCGTGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCATAGGCGTTATTTACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGtt                             <  2:762848/150‑1 (MQ=255)
                                                                                                                             ggCCTTTCAGACGGATGCGTTGACCATTTCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTACTGTTAGATCATTCCGAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTAATCTTATGCTCATTAAGCGTttatt                         >  2:1584241/1‑150 (MQ=255)
                                                                                                                              gCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTttcttc                        >  2:1123215/1‑150 (MQ=255)
                                                                                                                                  ttgaGACGGATGCGTTGACCTTTGCCGACGAGCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAgg                    <  1:83938/147‑1 (MQ=255)
                                                                                                                                  ttCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAgg                    <  1:1238664/150‑1 (MQ=255)
                                                                                                                                  ttCAGACGGATGCGGTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAgg                    <  1:560748/150‑1 (MQ=255)
                                                                                                                                      gACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATa                >  2:2026985/1‑150 (MQ=255)
                                                                                                                                      gACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATa                >  2:1716866/1‑150 (MQ=255)
                                                                                                                                         gtaTGCGTTTACCATTGCCGACGACCTCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCg             <  2:1944522/148‑1 (MQ=255)
                                                                                                                                          gATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGc            >  1:599880/1‑150 (MQ=255)
                                                                                                                                           aTGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGcc           <  1:1114830/150‑1 (MQ=255)
                                                                                                                                           aTGCGGTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTGTTCGGAATTTCCTGTTCGATCATGACAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGcc           <  2:1243110/150‑1 (MQ=255)
                                                                                                                                            tGCGTTGGCCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCa          >  2:819998/1‑150 (MQ=255)
                                                                                                                                            tGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCTCCa          <  2:580246/150‑1 (MQ=255)
                                                                                                                                               gTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACtt       >  2:717776/1‑150 (MQ=255)
                                                                                                                                                   aCCATTGCCGACGCCCGCCGGTATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGAt   <  2:104558/150‑1 (MQ=255)
                                                                                                                                                   aCCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGAt   >  1:1306420/1‑150 (MQ=255)
                                                                                                                                                    ccATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGAtt  >  2:371846/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACCAGCCACAAATCGCCATTTGGACCGCCGTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTTCGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATT  >  NC_000913/1063116‑1063413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑