Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,101,557 G→C 7.6% P43R (CCA→CGA) ‡ csgG ← curli production assembly/transport outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,101,5570GC 7.6% 358.5 / 19.5 118P43R (CCA→CGA) ‡csgGcurli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (59/49);  new base C (5/4);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.27e-01

Only 100 of 121 total aligned reads displayed.

ACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTG  >  NC_000913/1101408‑1101706
                                                                                                                                                     |                                                                                                                                                     
aCCAGCGAGAATCTTTCAGTGCCGGGACCAGCATTGCCGTGGCGCGTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAACTTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTg                                                                                                                                                       <  1:161199/150‑1 (MQ=255)
  cAGCGAGAAGCTTTCAGAGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGc                                                                                                                                                     <  2:523565/150‑1 (MQ=255)
   aGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCa                                                                                                                                                    >  1:983113/1‑150 (MQ=255)
    gCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAg                                                                                                                                                   >  2:1589047/1‑150 (MQ=255)
    gCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAg                                                                                                                                                   <  2:1545153/150‑1 (MQ=255)
       agaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATg                                                                                                                                                >  2:1968770/1‑150 (MQ=255)
       agaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATg                                                                                                                                                <  2:1417309/150‑1 (MQ=255)
         aaTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATggg                                                                                                                                              <  2:958233/150‑1 (MQ=255)
           tCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTc                                                                                                                                            <  1:689709/150‑1 (MQ=255)
             tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                          <  2:708920/150‑1 (MQ=255)
             tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGGCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                          <  2:784672/150‑1 (MQ=255)
               tCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAAt                                                                                                                                        <  1:508065/150‑1 (MQ=255)
               tCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAAt                                                                                                                                        <  2:1378656/150‑1 (MQ=255)
                  gTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCtt                                                                                                                                     >  1:181962/1‑150 (MQ=255)
                   tGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCCGCGCTGGCAGATGGGTCAAAGCttt                                                                                                                                    >  1:1044762/1‑150 (MQ=255)
                    gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                                   <  2:1191621/150‑1 (MQ=255)
                     ccctGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGt                                                                                                                                  <  1:1292387/147‑1 (MQ=25)
                     ccGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGt                                                                                                                                  <  1:1402739/150‑1 (MQ=37)
                       gTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCAGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTCCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAg                                                                                                                                >  2:1690248/1‑150 (MQ=255)
                         gACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGct                                                                                                                              >  2:1544034/1‑150 (MQ=255)
                            cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                           <  1:1817278/150‑1 (MQ=255)
                            cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                           <  2:1095288/150‑1 (MQ=255)
                             aGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGa                                                                                                                          <  2:1166663/150‑1 (MQ=255)
                                   gCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGa                                                                                                                    >  1:441307/1‑150 (MQ=255)
                                   gCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGa                                                                                                                    >  1:2023702/1‑150 (MQ=255)
                                    ccGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAg                                                                                                                   >  2:1707392/1‑150 (MQ=255)
                                      gTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCTCGAGGc                                                                                                                 >  1:1348093/1‑150 (MQ=255)
                                           gcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTat                                                                                                            >  1:374604/1‑149 (MQ=255)
                                            cTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGTGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAt                                                                                                           <  1:1708981/150‑1 (MQ=255)
                                              ttGCGGAACAGCAGTGGAGAAGTTACATGCCGGGTAGGGTTTAAATTGCCCGGAATCGTCCTGAATGTTGTATACCGAAACAAAGATTATACACGACGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGt                                                                                                         >  1:1127304/1‑150 (MQ=255)
                                                gCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCg                                                                                                       >  1:1871699/1‑150 (MQ=255)
                                                 cGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGGTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCgg                                                                                                      <  2:184693/150‑1 (MQ=255)
                                                   gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                    <  2:1620974/150‑1 (MQ=255)
                                                   gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                    <  2:618142/150‑1 (MQ=255)
                                                   gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                    >  1:938030/1‑150 (MQ=255)
                                                      cagcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTgg                                                                                                 >  1:642843/1‑150 (MQ=255)
                                                      cagcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTgg                                                                                                 >  1:1825547/1‑150 (MQ=255)
                                                        gcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcg                                                                                               >  1:1080154/1‑150 (MQ=255)
                                                         cagTTGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCTAAACAAAGATTTTACCCGTCGGCGCTGTCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcgg                                                                                              <  2:1836022/150‑1 (MQ=255)
                                                              gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCTTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                         <  2:1697113/150‑1 (MQ=255)
                                                              gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                         <  1:52760/150‑1 (MQ=255)
                                                              gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACACGTCGGCGCTTGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                         >  1:1243451/1‑150 (MQ=255)
                                                               agaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTCTATACCGAAACAAAGATTTTACACGTCGGCGCTGGCAGATGGGTCAAATATTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGCGGcttctt                                                                                        >  1:1847342/1‑150 (MQ=255)
                                                                gaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCttt                                                                                       >  1:445475/1‑150 (MQ=255)
                                                                  aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                                     >  1:937872/1‑150 (MQ=255)
                                                                   gTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAgg                                                                                    >  1:466280/1‑150 (MQ=255)
                                                                   gTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAgg                                                                                    <  1:1994034/150‑1 (MQ=255)
                                                                     tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTTTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                  >  1:1801953/1‑150 (MQ=255)
                                                                     tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                  >  2:1580397/1‑150 (MQ=255)
                                                                     tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                  >  2:926845/1‑150 (MQ=255)
                                                                     tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCAGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                  >  2:1113475/1‑150 (MQ=255)
                                                                       cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                                >  2:1998209/1‑150 (MQ=255)
                                                                       cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                                >  1:1989918/1‑150 (MQ=255)
                                                                        ttGCCGGGGAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGAATTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcgggg                                                                               <  1:453229/150‑1 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                              >  1:432180/1‑150 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                              <  1:589239/150‑1 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                              >  2:287667/1‑150 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                              <  1:680953/150‑1 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCGTTTggcggggc                                                                              >  2:160210/1‑150 (MQ=255)
                                                                         tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGGCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                              <  1:452616/150‑1 (MQ=255)
                                                                           ccGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggcgg                                                                            >  2:594922/1‑150 (MQ=255)
                                                                            cGGGTAGGGTTTAATTTTCCCGGTGTCGTCCTGAATGTTGTATACCGAAACAAAGATATTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                           <  2:386377/150‑1 (MQ=255)
                                                                            cGGGTAGGGTTTAAATTGCCCTGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCTGCTTCTTTAGGCGGGGCGGt                                                                           <  2:1709909/150‑1 (MQ=255)
                                                                            cGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                           >  2:1130069/1‑150 (MQ=255)
                                                                             gggTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGtt                                                                          >  1:1850029/1‑150 (MQ=255)
                                                                             gggTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGtt                                                                          <  2:1331293/150‑1 (MQ=255)
                                                                                tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGCGGCGGTTAAg                                                                       >  1:1800914/1‑150 (MQ=255)
                                                                                tAGGGGTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAg                                                                       <  1:791000/150‑1 (MQ=255)
                                                                                  gggTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCa                                                                     <  1:868770/150‑1 (MQ=255)
                                                                                  gggTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCa                                                                     >  1:913232/1‑150 (MQ=255)
                                                                                                ctggttagTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCTCTGGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGAGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACa                                                       <  2:191114/143‑1 (MQ=255)
                                                                                                      cGTCCTGATTGGTGTATAACGCAACAAAGATTTTACCCGGCGGCGCTGGCATCTGGGTAAAATATTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACgg                                                 <  1:186564/150‑1 (MQ=255)
                                                                                                        tCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCa                                               >  2:1305079/1‑150 (MQ=255)
                                                                                                           tGAATGTTGTATACCGAAACAAAGATTTAACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAcc                                            <  1:391695/150‑1 (MQ=255)
                                                                                                            gAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCa                                           <  2:355849/150‑1 (MQ=255)
                                                                                                                gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAg                                       <  2:1595184/150‑1 (MQ=255)
                                                                                                                gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCATTGTAGCTCAGAGCACGAGGCATTAATGACGGACTGGCGGCTTCATAAGGAGGGGCGGTTAAGCATCCGCACACTTAGATAACGGCACCCAAAAg                                       >  2:1329262/1‑150 (MQ=255)
                                                                                                                 ttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGa                                      <  1:990471/150‑1 (MQ=255)
                                                                                                                     ataCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATa                                  <  1:1238306/150‑1 (MQ=255)
                                                                                                                      taCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATaa                                 >  2:1963456/1‑150 (MQ=255)
                                                                                                                        gagctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                               >  1:1069073/6‑150 (MQ=25)
                                                                                                                        ccGAAACAAAGATTTTACCCGTCGGCGCTGGCTGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTTGGCGGGGCGTTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                               >  2:302918/1‑150 (MQ=255)
                                                                                                                        ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                               >  1:2026471/1‑150 (MQ=255)
                                                                                                                        ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGAAGCTCAGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                               >  2:279098/1‑150 (MQ=255)
                                                                                                                           ctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCt                            >  1:1432893/3‑150 (MQ=37)
                                                                                                                            aaCAAAGATTTTACCCGTCGGCGCTGGCAGATGCGTCAAATCTTTGTAGCTCTGATCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGATAAGCATCAGCACAGTAAGATCACGGCAACCAAATGAAATAAGCTCTg                           >  1:313573/1‑150 (MQ=255)
                                                                                                                              cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                         >  1:916351/1‑150 (MQ=255)
                                                                                                                              cAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCTGGGCGCTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                         >  2:1086376/1‑150 (MQ=37)
                                                                                                                               caaGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTGCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCAt                        <  2:354810/149‑1 (MQ=255)
                                                                                                                                      ttACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGattatt                 <  1:1655583/150‑1 (MQ=255)
                                                                                                                                      ttACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGattatt                 >  1:516353/1‑150 (MQ=255)
                                                                                                                                        aCCCGGCGGCGCTGGCAGCTGGGTCAAATCTTTGTAGATCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTAtttt               <  2:1243892/150‑1 (MQ=255)
                                                                                                                                         cccGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTc              >  1:1172295/1‑150 (MQ=255)
                                                                                                                                           cGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCt            >  1:896188/1‑150 (MQ=255)
                                                                                                                                            gTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCtt           <  2:441307/150‑1 (MQ=255)
                                                                                                                                             tCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTa          <  1:1998210/150‑1 (MQ=255)
                                                                                                                                               ggCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATg        <  2:1989917/150‑1 (MQ=255)
                                                                                                                                                   cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGATCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc    <  1:287667/150‑1 (MQ=255)
                                                                                                                                                   cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCGTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc    >  1:111860/1‑150 (MQ=255)
                                                                                                                                                     ggCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTg  >  2:1510059/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCAGCGAGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTG  >  NC_000913/1101408‑1101706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: