Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,101,563 T→C 7.7% H41R (CAT→CGT)  csgG ← curli production assembly/transport outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,101,5630TC 7.7% 334.5 / 14.0 116H41R (CAT→CGT) csgGcurli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (60/47);  new base C (5/4);  total (65/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 117 total aligned reads displayed.

AGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCTT  >  NC_000913/1101415‑1101712
                                                                                                                                                    |                                                                                                                                                     
agaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATg                                                                                                                                                      <  2:1417309/150‑1 (MQ=255)
agaATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATg                                                                                                                                                      >  2:1968770/1‑150 (MQ=255)
  aaTCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATggg                                                                                                                                                    <  2:958233/150‑1 (MQ=255)
    tCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTc                                                                                                                                                  <  1:689709/150‑1 (MQ=255)
      tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                                <  2:708920/150‑1 (MQ=255)
      tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGGCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                                <  2:784672/150‑1 (MQ=255)
        tCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAAt                                                                                                                                              <  2:1378656/150‑1 (MQ=255)
        tCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAAt                                                                                                                                              <  1:508065/150‑1 (MQ=255)
           gTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCtt                                                                                                                                           >  1:181962/1‑150 (MQ=255)
            tGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCCGCGCTGGCAGATGGGTCAAAGCttt                                                                                                                                          >  1:1044762/1‑150 (MQ=255)
             gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                                         <  1:438878/150‑1 (MQ=255)
             gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                                         <  2:1191621/150‑1 (MQ=255)
              ccctGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGt                                                                                                                                        <  1:1292387/147‑1 (MQ=25)
              ccGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGt                                                                                                                                        <  1:1402739/150‑1 (MQ=37)
                gTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGttt                                                                                                                                      <  2:2033213/150‑3 (MQ=37)
                gTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCAGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTCCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAg                                                                                                                                      >  2:1690248/1‑150 (MQ=255)
                gTGACCAGCATTGCCGTGGCGCTTTGCGGAAAAGCAGTGGACAAGTTACTTGCCGGGTAGGGTTGAAATTGCCCTGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTAAAATCTTTGTAg                                                                                                                                      <  2:1594093/150‑1 (MQ=255)
                  gACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAACCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGtttcg                                                                                                                                    <  1:211758/150‑5 (MQ=25)
                  gACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGct                                                                                                                                    >  2:1544034/1‑150 (MQ=255)
                     cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                                 <  2:1095288/150‑1 (MQ=255)
                     cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                                 <  1:1817278/150‑1 (MQ=255)
                      aGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGa                                                                                                                                <  2:1166663/150‑1 (MQ=255)
                            gCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGa                                                                                                                          >  1:441307/1‑150 (MQ=255)
                            gCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGa                                                                                                                          >  1:2023702/1‑150 (MQ=255)
                             ccGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAg                                                                                                                         >  2:1707392/1‑150 (MQ=255)
                               gTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCTCGAGGc                                                                                                                       >  1:1348093/1‑150 (MQ=255)
                                     cTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGTGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAt                                                                                                                 <  1:1708981/150‑1 (MQ=255)
                                       ttGCGGAACAGCAGTGGAGAAGTTACATGCCGGGTAGGGTTTAAATTGCCCGGAATCGTCCTGAATGTTGTATACCGAAACAAAGATTATACACGACGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGt                                                                                                               >  1:1127304/1‑150 (MQ=255)
                                        tGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTc                                                                                                              >  2:1500816/1‑150 (MQ=255)
                                         gCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCg                                                                                                             >  1:1871699/1‑150 (MQ=255)
                                          cGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGGTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCgg                                                                                                            <  2:184693/150‑1 (MQ=255)
                                            gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                          <  2:1620974/150‑1 (MQ=255)
                                               cagcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTgg                                                                                                       >  1:642843/1‑150 (MQ=255)
                                               cagcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTgg                                                                                                       >  1:1825547/1‑150 (MQ=255)
                                                agcagTGGAGAAGTTACTTGCCGGGTTGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGTTTTTACCCGTCGGCGCTGTCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggc                                                                                                      <  1:943151/150‑1 (MQ=255)
                                                 gcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcg                                                                                                     >  1:1080154/1‑150 (MQ=255)
                                                  cagTTGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCTAAACAAAGATTTTACCCGTCGGCGCTGTCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcgg                                                                                                    <  2:1836022/150‑1 (MQ=255)
                                                   agTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggctga                                                                                                   >  2:386172/1‑147 (MQ=255)
                                                       gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCTTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                               <  2:1697113/150‑1 (MQ=255)
                                                       gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                               <  1:52760/150‑1 (MQ=255)
                                                       gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACACGTCGGCGCTTGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                               >  1:1243451/1‑150 (MQ=255)
                                                        agaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTCTATACCGAAACAAAGATTTTACACGTCGGCGCTGGCAGATGGGTCAAATATTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGCGGcttctt                                                                                              >  1:1847342/1‑150 (MQ=255)
                                                         gaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCttt                                                                                             >  1:445475/1‑150 (MQ=255)
                                                           aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                                           >  1:1958343/1‑150 (MQ=255)
                                                           aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                                           >  1:937872/1‑150 (MQ=255)
                                                            gTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAgg                                                                                          >  1:466280/1‑150 (MQ=255)
                                                              tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTTTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                        >  1:1801953/1‑150 (MQ=255)
                                                              tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                        >  2:926845/1‑150 (MQ=255)
                                                              tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCAGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                        >  2:1113475/1‑150 (MQ=255)
                                                                cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                                      >  1:1989918/1‑150 (MQ=255)
                                                                cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                                      >  2:1998209/1‑150 (MQ=255)
                                                                  tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                    <  1:680953/150‑1 (MQ=255)
                                                                  tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                    >  2:287667/1‑150 (MQ=255)
                                                                  tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                    >  1:432180/1‑150 (MQ=255)
                                                                  tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCGTTTggcggggc                                                                                    >  2:160210/1‑150 (MQ=255)
                                                                  tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGGCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                    <  1:452616/150‑1 (MQ=255)
                                                                    ccGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggcgg                                                                                  >  2:594922/1‑150 (MQ=255)
                                                                     cGGGTAGGGTTTAATTTTCCCGGTGTCGTCCTGAATGTTGTATACCGAAACAAAGATATTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                                 <  2:386377/150‑1 (MQ=255)
                                                                     cGGGTAGGGTTTAAATTGCCCTGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCTGCTTCTTTAGGCGGGGCGGt                                                                                 <  2:1709909/150‑1 (MQ=255)
                                                                     cGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                                 >  2:1130069/1‑150 (MQ=255)
                                                                      gggTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGtt                                                                                <  2:1331293/150‑1 (MQ=255)
                                                                      gggTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGtt                                                                                >  1:1850029/1‑150 (MQ=255)
                                                                         tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGCGGCGGTTAAg                                                                             >  1:1800914/1‑150 (MQ=255)
                                                                         tAGGGGTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAg                                                                             <  1:791000/150‑1 (MQ=255)
                                                                           gggTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCa                                                                           >  1:913232/1‑150 (MQ=255)
                                                                             gTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATc                                                                         >  2:686828/1‑150 (MQ=255)
                                                                                  aaTTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTc                                                                    >  1:1171615/1‑150 (MQ=255)
                                                                                         ctggttagTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCTCTGGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGAGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACa                                                             <  2:191114/143‑1 (MQ=255)
                                                                                               cGTCCTGATTGGTGTATAACGCAACAAAGATTTTACCCGGCGGCGCTGGCATCTGGGTAAAATATTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACgg                                                       <  1:186564/150‑1 (MQ=255)
                                                                                                 tCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCa                                                     >  2:1305079/1‑150 (MQ=255)
                                                                                                    tGAATGTTGTATACCGAAACAAAGATTTAACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAcc                                                  <  1:391695/150‑1 (MQ=255)
                                                                                                     gAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCa                                                 <  2:355849/150‑1 (MQ=255)
                                                                                                         gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAg                                             <  2:1595184/150‑1 (MQ=255)
                                                                                                         gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAg                                             >  2:1858903/1‑150 (MQ=255)
                                                                                                          ttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGa                                            <  1:990471/150‑1 (MQ=255)
                                                                                                              ataCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATa                                        <  1:1238306/150‑1 (MQ=255)
                                                                                                                 gagctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                     >  1:1069073/6‑150 (MQ=25)
                                                                                                                 gagctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                     >  1:602617/6‑150 (MQ=25)
                                                                                                                 ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                     >  1:2026471/1‑150 (MQ=255)
                                                                                                                 ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGAAGCTCAGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                     >  2:279098/1‑150 (MQ=255)
                                                                                                                   gctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgcgc                                   >  1:486181/4‑150 (MQ=25)
                                                                                                                    ctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCt                                  >  1:1432893/3‑150 (MQ=37)
                                                                                                                     aaCAAAGATTTTACCCGTCGGCGCTGGCAGATGCGTCAAATCTTTGTAGCTCTGATCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGATAAGCATCAGCACAGTAAGATCACGGCAACCAAATGAAATAAGCTCTg                                 >  1:313573/1‑150 (MQ=255)
                                                                                                                       cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                               >  2:1298134/1‑150 (MQ=255)
                                                                                                                       cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                               >  1:916351/1‑150 (MQ=255)
                                                                                                                       cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                               >  1:875977/1‑150 (MQ=255)
                                                                                                                       cAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCTGGGCGCTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                               >  2:1086376/1‑150 (MQ=37)
                                                                                                                        caaGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTGCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCAt                              <  2:354810/149‑1 (MQ=255)
                                                                                                                               ttACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGattatt                       >  1:516353/1‑150 (MQ=255)
                                                                                                                                 aCCCGGCGGCGCTGGCAGCTGGGTCAAATCTTTGTAGATCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTAtttt                     <  2:1243892/150‑1 (MQ=255)
                                                                                                                                    cGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCt                  >  1:896188/1‑150 (MQ=255)
                                                                                                                                     gTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCtt                 <  2:441307/150‑1 (MQ=255)
                                                                                                                                      tCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTa                <  1:1998210/150‑1 (MQ=255)
                                                                                                                                        ggCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATg              <  2:1989917/150‑1 (MQ=255)
                                                                                                                                            cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGATCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc          <  1:287667/150‑1 (MQ=255)
                                                                                                                                            cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCGTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc          >  1:111860/1‑150 (MQ=255)
                                                                                                                                              ggCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTg        >  2:1510059/1‑150 (MQ=255)
                                                                                                                                                  gATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGc    >  1:964090/1‑150 (MQ=255)
                                                                                                                                                  gATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGc    >  1:556301/1‑150 (MQ=255)
                                                                                                                                                    tGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGCCGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCtt  >  1:738962/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGAATCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCTT  >  NC_000913/1101415‑1101712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: