Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,101,568 C→A 8.6% L39F (TTG→TTT csgG ← curli production assembly/transport outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,101,5680CA 8.6% 330.7 / 14.7 118L39F (TTG→TTTcsgGcurli production assembly/transport outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (64/43);  new base A (5/6);  total (69/49)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 119 total aligned reads displayed.

TCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCTTAAAA  >  NC_000913/1101419‑1101716
                                                                                                                                                     |                                                                                                                                                    
tCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTc                                                                                                                                                      <  1:689709/150‑1 (MQ=255)
  tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                                    <  2:708920/150‑1 (MQ=255)
  tttCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGGCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCaa                                                                                                                                                    <  2:784672/150‑1 (MQ=255)
    tCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAAt                                                                                                                                                  <  1:508065/150‑1 (MQ=255)
    tCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAAt                                                                                                                                                  <  2:1378656/150‑1 (MQ=255)
        tGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCCGCGCTGGCAGATGGGTCAAAGCttt                                                                                                                                              >  1:1044762/1‑150 (MQ=255)
         gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                                             <  1:438878/150‑1 (MQ=255)
         gCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTg                                                                                                                                             <  2:1191621/150‑1 (MQ=255)
          ccctGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGt                                                                                                                                            <  1:1292387/147‑1 (MQ=25)
          ccGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGt                                                                                                                                            <  1:1402739/150‑1 (MQ=37)
            gTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGttt                                                                                                                                          <  2:2033213/150‑3 (MQ=37)
            gTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCAGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTCCCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAg                                                                                                                                          >  2:1690248/1‑150 (MQ=255)
            gTGACCAGCATTGCCGTGGCGCTTTGCGGAAAAGCAGTGGACAAGTTACTTGCCGGGTAGGGTTGAAATTGCCCTGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTAAAATCTTTGTAg                                                                                                                                          <  2:1594093/150‑1 (MQ=255)
              gACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAACCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGtttcg                                                                                                                                        <  1:211758/150‑5 (MQ=25)
              gACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGct                                                                                                                                        >  2:1544034/1‑150 (MQ=255)
                 cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                                     <  2:1095288/150‑1 (MQ=255)
                 cAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTg                                                                                                                                     <  1:1817278/150‑1 (MQ=255)
                  aGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGa                                                                                                                                    <  2:1166663/150‑1 (MQ=255)
                        gCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGa                                                                                                                              >  1:441307/1‑150 (MQ=255)
                         ccGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAg                                                                                                                             >  2:1707392/1‑150 (MQ=255)
                           gTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCTCGAGGc                                                                                                                           >  1:1348093/1‑150 (MQ=255)
                                gcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTat                                                                                                                      >  1:374604/1‑149 (MQ=255)
                                gcTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCAAGAGGCATTaa                                                                                                                      >  2:1124613/1‑150 (MQ=255)
                                 cTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGTGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAAt                                                                                                                     <  1:1708981/150‑1 (MQ=255)
                                   ttGCGGAACAGCAGTGGAGAAGTTACATGCCGGGTAGGGTTTAAATTGCCCGGAATCGTCCTGAATGTTGTATACCGAAACAAAGATTATACACGACGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGt                                                                                                                   >  1:1127304/1‑150 (MQ=255)
                                      cGGAACAGCAGTGGAGAAGTTACTTGCCGGGGAGGGTTTAAATTGCCCGGGTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCgg                                                                                                                <  2:184693/150‑1 (MQ=255)
                                        gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                              <  2:1620974/150‑1 (MQ=255)
                                        gAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTc                                                                                                              >  1:938030/1‑150 (MQ=255)
                                           cagcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTgg                                                                                                           >  1:642843/1‑150 (MQ=255)
                                           cagcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTgg                                                                                                           >  1:1825547/1‑150 (MQ=255)
                                            agcagTGGAGAAGTTACTTGCCGGGTTGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGTTTTTACCCGTCGGCGCTGTCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggc                                                                                                          <  1:943151/150‑1 (MQ=255)
                                             gcagTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcg                                                                                                         >  1:1080154/1‑150 (MQ=255)
                                              cagTTGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCTAAACAAAGATTTTACCCGTCGGCGCTGTCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggcgg                                                                                                        <  2:1836022/150‑1 (MQ=255)
                                               agTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTggctga                                                                                                       >  2:386172/1‑147 (MQ=255)
                                                   gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCTTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                                   <  2:1697113/150‑1 (MQ=255)
                                                   gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                                   <  1:52760/150‑1 (MQ=255)
                                                   gagaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACACGTCGGCGCTTGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGcttct                                                                                                   >  1:1243451/1‑150 (MQ=255)
                                                    agaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTCTATACCGAAACAAAGATTTTACACGTCGGCGCTGGCAGATGGGTCAAATATTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGCGGcttctt                                                                                                  >  1:1847342/1‑150 (MQ=255)
                                                     gaAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCttt                                                                                                 >  1:445475/1‑150 (MQ=255)
                                                       aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                                               >  1:937872/1‑150 (MQ=255)
                                                       aGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAg                                                                                               >  1:1958343/1‑150 (MQ=255)
                                                        gTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAgg                                                                                              >  1:466280/1‑150 (MQ=255)
                                                        gTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAgg                                                                                              <  1:1994034/150‑1 (MQ=255)
                                                          tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTTTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                            >  1:1801953/1‑150 (MQ=255)
                                                          tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                            >  2:926845/1‑150 (MQ=255)
                                                          tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                            >  2:1580397/1‑150 (MQ=255)
                                                          tACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCAGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcg                                                                                            >  2:1113475/1‑150 (MQ=255)
                                                            cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                                          >  2:1998209/1‑150 (MQ=255)
                                                            cTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggg                                                                                          >  1:1989918/1‑150 (MQ=255)
                                                              tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                        >  1:432180/1‑150 (MQ=255)
                                                              tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                        >  2:287667/1‑150 (MQ=255)
                                                              tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                        <  1:680953/150‑1 (MQ=255)
                                                              tGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGGCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggc                                                                                        <  1:452616/150‑1 (MQ=255)
                                                                ccGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAggcggggcgg                                                                                      >  2:594922/1‑150 (MQ=255)
                                                                 cGGGTAGGGTTTAATTTTCCCGGTGTCGTCCTGAATGTTGTATACCGAAACAAAGATATTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                                     <  2:386377/150‑1 (MQ=255)
                                                                 cGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGt                                                                                     >  2:1130069/1‑150 (MQ=255)
                                                                  gggTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGtt                                                                                    >  1:1850029/1‑150 (MQ=255)
                                                                  gggTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGtt                                                                                    <  2:1331293/150‑1 (MQ=255)
                                                                     tAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGCGGCGGTTAAg                                                                                 >  1:1800914/1‑150 (MQ=255)
                                                                       gggTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCa                                                                               >  1:913232/1‑150 (MQ=255)
                                                                       gggTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCa                                                                               <  1:868770/150‑1 (MQ=255)
                                                                         gTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATc                                                                             >  2:686828/1‑150 (MQ=255)
                                                                              aaTTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTc                                                                        >  1:1171615/1‑150 (MQ=255)
                                                                                     ctggttagTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCTCTGGCAGATGGTTCAAATCTTTGTAGCTCTGAGCACGCGGCATTAATGTCGGTCTGGAGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACa                                                                 <  2:191114/143‑1 (MQ=255)
                                                                                           cGTCCTGATTGGTGTATAACGCAACAAAGATTTTACCCGGCGGCGCTGGCATCTGGGTAAAATATTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACgg                                                           <  1:186564/150‑1 (MQ=255)
                                                                                             tCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCa                                                         >  2:1305079/1‑150 (MQ=255)
                                                                                                 gAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCa                                                     <  2:355849/150‑1 (MQ=255)
                                                                                                     gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAg                                                 >  2:1858903/1‑150 (MQ=255)
                                                                                                     gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAg                                                 <  2:1595184/150‑1 (MQ=255)
                                                                                                     gttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCATTGTAGCTCAGAGCACGAGGCATTAATGACGGACTGGCGGCTTCATAAGGAGGGGCGGTTAAGCATCCGCACACTTAGATAACGGCACCCAAAAg                                                 >  2:1329262/1‑150 (MQ=255)
                                                                                                      ttgtATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGa                                                <  1:990471/150‑1 (MQ=255)
                                                                                                          ataCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATa                                            <  1:1238306/150‑1 (MQ=255)
                                                                                                           taCCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATaa                                           >  2:1963456/1‑150 (MQ=255)
                                                                                                             gagctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                         >  1:1069073/6‑150 (MQ=25)
                                                                                                             gagctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                         >  1:602617/6‑150 (MQ=25)
                                                                                                             ccGAAACAAAGATTTTACCCGTCGGCGCTGGCTGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTTGGCGGGGCGTTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                         >  2:302918/1‑150 (MQ=255)
                                                                                                             ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                         >  1:2026471/1‑150 (MQ=255)
                                                                                                             ccGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGAAGCTCAGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgc                                         >  2:279098/1‑150 (MQ=255)
                                                                                                               gctaCAAAGATTTGACCCATCTGCCAGCGCCGACGGGTAAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAgcgc                                       >  1:486181/4‑150 (MQ=25)
                                                                                                                 aaCAAAGATTTTACCCGTCGGCGCTGGCAGATGCGTCAAATCTTTGTAGCTCTGATCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGATAAGCATCAGCACAGTAAGATCACGGCAACCAAATGAAATAAGCTCTg                                     >  1:313573/1‑150 (MQ=255)
                                                                                                                   cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                                   >  2:1298134/1‑150 (MQ=255)
                                                                                                                   cAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCa                                   >  1:875977/1‑150 (MQ=255)
                                                                                                                           ttACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGattatt                           <  1:1655583/150‑1 (MQ=255)
                                                                                                                           ttACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGattatt                           >  1:516353/1‑150 (MQ=255)
                                                                                                                               ccGGCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTcc                       <  1:1842195/150‑1 (MQ=255)
                                                                                                                                cGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCt                      >  1:896188/1‑150 (MQ=255)
                                                                                                                                 gTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCtt                     <  2:441307/150‑1 (MQ=255)
                                                                                                                                  tCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTa                    <  1:1998210/150‑1 (MQ=255)
                                                                                                                                        cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGATCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc              <  1:287667/150‑1 (MQ=255)
                                                                                                                                        cTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCGTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGc              >  1:111860/1‑150 (MQ=255)
                                                                                                                                          ggCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCATAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTg            >  2:1702228/1‑150 (MQ=255)
                                                                                                                                          ggCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTg            >  2:1510059/1‑150 (MQ=255)
                                                                                                                                              gATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGc        >  1:964090/1‑150 (MQ=255)
                                                                                                                                              gATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGc        >  1:556301/1‑150 (MQ=255)
                                                                                                                                                tGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGCCGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCtt      >  1:738962/1‑150 (MQ=255)
                                                                                                                                                 gggTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAAACAAAAGAAATAAGCGCTTCATGATTATTTTCCTTATGAAGCTGGGGCTTa     <  2:820154/150‑1 (MQ=255)
                                                                                                                                                   gTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAAGATGACGGCAACCAAAAGAAATAAGCGCTGCAGGATTATTTTCCTTATGAAGCGGGGGGTTaaa   >  2:1929771/1‑150 (MQ=255)
                                                                                                                                                   gTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCTTaaa   >  1:879649/1‑150 (MQ=255)
                                                                                                                                                   gTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCTTaaa   >  1:1849792/1‑150 (MQ=255)
                                                                                                                                                    tCAAATCTTTGTAGCTCTGAGCACGAGGCAATAATGTCGGTCTTGCGGCTTCTTTAGGCGGGCCGGTTAAGCAACCGCTCAGTAACATGACGGCAACCAAAAGAAAAAAGAGAGGCATGATAATTTTCCTTATAAAGCTGGCGCTTaaaa  >  1:1912394/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTTTCAGTGCCGTGACCAGCATTGCCGTGGCGCTTTGCGGAACAGCAGTGGAGAAGTTACTTGCCGGGTAGGGTTTAAATTGCCCGGTTTCGTCCTGAATGTTGTATACCGAAACAAAGATTTTACCCGTCGGCGCTGGCAGATGGGTCAAATCTTTGTAGCTCTGAGCACGAGGCATTAATGTCGGTCTGGCGGCTTCTTTAGGCGGGGCGGTTAAGCATCCGCTCAGTAACATGACGGCAACCAAAAGAAATAAGCGCTGCATGATTATTTTCCTTATGAAGCTGGGGCTTAAAA  >  NC_000913/1101419‑1101716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: