Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,134,662 A→C 6.0% intergenic (+105/‑67) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,134,6620AC 6.0% 314.6 / 6.6 104intergenic (+105/‑67)flgF/flgGflagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base A (58/39);  new base C (2/5);  total (60/44)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAA  >  NC_000913/1134515‑1134811
                                                                                                                                                   |                                                                                                                                                     
tgatAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAgg                                                                                                                                                     <  1:965608/150‑1 (MQ=255)
tgatAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAgg                                                                                                                                                     <  1:439298/150‑1 (MQ=255)
   tAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGt                                                                                                                                                  >  2:1059154/1‑150 (MQ=255)
          gtccGTGCCAACCAACTGCTGTCGATGAGGTAATTGAAAGGATACCTGACAAGGATAAGTTGCCCGATGCGCAAGTGTATCGGGTCTATGGGTGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  1:102514/148‑1 (MQ=255)
           gCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  1:389908/150‑1 (MQ=255)
           gCCGTGCCAACCAACTGCTGTCGAGGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  2:1187615/150‑1 (MQ=255)
                gCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                     <  2:1586703/150‑1 (MQ=255)
                 ccaaccaaCTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                    >  2:901884/1‑150 (MQ=255)
                  caaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                   >  2:569271/1‑150 (MQ=255)
                   aaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  >  1:458997/1‑150 (MQ=255)
                   aaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  <  1:1532482/150‑1 (MQ=255)
                   aaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTATATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGc                                                                                                                                  >  2:981125/1‑150 (MQ=255)
                    accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCTGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                 >  1:1569071/1‑150 (MQ=255)
                    accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                 <  1:725073/150‑2 (MQ=255)
                    accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                 <  1:237602/150‑2 (MQ=255)
                     ccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                                                                                                                <  1:871686/150‑3 (MQ=255)
                      caacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaag                                                                                                                               <  1:512718/150‑1 (MQ=255)
                        acTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaagaa                                                                                                                             <  1:1827130/150‑1 (MQ=255)
                        acTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaagaa                                                                                                                             >  2:1585472/1‑150 (MQ=255)
                         ctgctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaagaag                                                                                                                            >  2:1265586/1‑150 (MQ=255)
                           gctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                          >  2:1259815/1‑150 (MQ=255)
                            ctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                                                                                                         >  1:1013269/1‑150 (MQ=255)
                               tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACatat                                                                                                                      >  2:1863772/1‑150 (MQ=255)
                               tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACatat                                                                                                                      <  2:488052/150‑1 (MQ=255)
                                 gATGAGTTAATTGAAAGGATACATGTCAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGc                                                                                                                    >  1:140547/1‑150 (MQ=255)
                                 gATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGc                                                                                                                    <  2:1429627/150‑1 (MQ=255)
                                  aTGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCa                                                                                                                   <  1:919114/150‑1 (MQ=255)
                                     aGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACtt                                                                                                                >  2:1877183/1‑150 (MQ=255)
                                      gTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACttt                                                                                                               >  1:1084978/1‑150 (MQ=255)
                                        tAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTAACGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACAAGACATATGCACTATGt                                                                                                             >  1:1082848/1‑150 (MQ=255)
                                         aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc                                                                                                            >  1:1208722/1‑150 (MQ=255)
                                         aaTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc                                                                                                            <  1:1447683/150‑1 (MQ=255)
                                           ttGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAAGAAGACATATGCACTTTGTCAc                                                                                                          >  2:2030088/1‑150 (MQ=255)
                                           ttGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCAc                                                                                                          <  1:1658953/150‑1 (MQ=255)
                                           ttGAAAGGATACAAGACAAGTATAAGTTGCCCGATGCGAAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGAGAGCACTTGTAGGCCGGAAACGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCAc                                                                                                          >  1:1481883/1‑150 (MQ=255)
                                            tGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTTCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACt                                                                                                         <  2:1428563/150‑1 (MQ=255)
                                            tGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACt                                                                                                         >  2:1741758/1‑150 (MQ=255)
                                            tGAAAGGATACATGACAAGTATAAGTTGCCCGAAGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAAATAAACGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAAGAAGACATATGCACTTTGTCaat                                                                                                         >  1:1701170/1‑148 (MQ=39)
                                             gAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTa                                                                                                        >  2:101163/1‑150 (MQ=255)
                                             gAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTa                                                                                                        >  2:1580637/1‑150 (MQ=255)
                                              aaaGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTaa                                                                                                       >  2:508401/1‑150 (MQ=255)
                                                 ggATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTAATCGATTTTGAGAGCACTTGTAGGCCGGATAAGGAGTTTACGCCGCATCCGGCAAGAAGACATAACAACTTTGTCACTAAAcc                                                                                                    >  1:1142325/1‑150 (MQ=39)
                                                   aTACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCAc                                                                                                  <  2:1735786/150‑1 (MQ=255)
                                                    tACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACt                                                                                                 >  2:1184526/1‑150 (MQ=255)
                                                    aaCATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACt                                                                                                 <  1:1615879/149‑1 (MQ=255)
                                                        tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAg                                                                                             >  2:1823968/1‑150 (MQ=255)
                                                           cAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGAc                                                                                          >  1:444033/1‑150 (MQ=255)
                                                           cAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGAc                                                                                          >  1:1042944/1‑150 (MQ=255)
                                                             aGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAt                                                                                        >  1:1218462/1‑150 (MQ=255)
                                                             aGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAt                                                                                        >  2:465472/1‑150 (MQ=255)
                                                             aGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCCCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCCCTACAGGCCAt                                                                                        >  1:1035717/1‑150 (MQ=255)
                                                              gTATAATTTGCACGATGAGCAAGTTTATCTGTTCTATGGTGGCAATCTCAATTTATCTATTTTGCGAGAACTTGTAGGCCGGATAAGTCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAtt                                                                                       <  2:1182087/150‑1 (MQ=38)
                                                                ataAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAtttt                                                                                     >  2:1379151/1‑150 (MQ=255)
                                                                      tGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATc                                                                               <  1:575322/150‑1 (MQ=255)
                                                                      tGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATc                                                                               <  1:1002080/150‑1 (MQ=255)
                                                                      tGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATc                                                                               <  1:3687/150‑1 (MQ=255)
                                                                        cccGATGCGCAAGTTTCTCGGGGCTATGGGGGCAATCGCAATTTATCGATTTTTCGAGCACTTGTAGGCCGTATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAg                                                                             <  2:271833/150‑1 (MQ=255)
                                                                        cccGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCTCTAATCATCTACAGGACATTTTATGAACAg                                                                             >  1:1818880/1‑150 (MQ=255)
                                                                                gcAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAAGCCACTACAGGACATTTTATGATCAGTTAATTAt                                                                     >  1:1055646/1‑150 (MQ=255)
                                                                                  aaGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTACTCCACTACAGGACATTTTATGATCAGTTCATTATgg                                                                   <  2:459702/150‑1 (MQ=255)
                                                                                   aGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGa                                                                  <  1:1265587/150‑1 (MQ=255)
                                                                                        aTCGGTTCTATTGGTGAAATCGCAAGTTATCGATTTTGCGAGCACTTGTAGGCCGGATAACGCGTTTACGCAGCCTCCGGCAAGAAGACATATGCCCTTTTTCAATAATCCACTAAATGACATTTTATTATCAGTTCATTATGGATCGcc                                                             <  2:121366/150‑1 (MQ=255)
                                                                                         tCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCa                                                            >  2:2024375/1‑150 (MQ=255)
                                                                                          cGGGTCTATGGGGGCAATCGCAATATATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCCCTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaa                                                           >  2:357825/1‑150 (MQ=255)
                                                                                             gTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAAc                                                        <  2:449556/150‑1 (MQ=255)
                                                                                                 aTGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGc                                                    <  2:1013268/150‑1 (MQ=255)
                                                                                                  tGGGGGCAATAGCAATTTATCGAATTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGcc                                                   <  2:1929036/150‑1 (MQ=255)
                                                                                                   gggggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCt                                                  >  2:1666975/1‑150 (MQ=255)
                                                                                                     gggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTg                                                >  2:931379/1‑150 (MQ=255)
                                                                                                     gggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTg                                                <  2:1746416/150‑1 (MQ=255)
                                                                                                     gggCAATCGAAATTTATCGATTTTGCGAGCACTTGTAGGCCGGTTACGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGTTCAGTTCATTATGGATCGCCAAAACGGGCCTTg                                                <  2:827168/150‑1 (MQ=255)
                                                                                                      ggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGa                                               >  2:379150/1‑150 (MQ=255)
                                                                                                        cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACg                                             <  1:1259816/150‑1 (MQ=255)
                                                                                                        cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCACCAAAACGGGCCTTGACg                                             >  1:1794149/1‑150 (MQ=255)
                                                                                                         aaTCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGc                                            >  1:225086/1‑150 (MQ=255)
                                                                                                           tCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCTTTATGGATCGCCAAAACGGGCCTTGACGccc                                          >  1:1071664/1‑150 (MQ=255)
                                                                                                           tCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGccc                                          <  1:568587/150‑1 (MQ=255)
                                                                                                                 tttATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCaaa                                    >  1:720806/1‑150 (MQ=255)
                                                                                                                   tATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAAcc                                  <  1:175933/150‑1 (MQ=255)
                                                                                                                       gATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAata                              >  2:423576/1‑150 (MQ=255)
                                                                                                                         ttttGCGAGCACTTGTAGGCCGGATCAGGCGTTTACGCCGCCTCCGGCAAGAAGACATATGCACTTTGTCACTACTCCACTACAGGACATGTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATg                            >  1:296538/1‑150 (MQ=255)
                                                                                                                           ttGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGa                          <  1:1014847/150‑1 (MQ=255)
                                                                                                                           ttGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGa                          >  2:548390/1‑150 (MQ=255)
                                                                                                                           ttGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGa                          <  1:152177/150‑1 (MQ=255)
                                                                                                                              cGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGt                       >  1:223740/1‑150 (MQ=255)
                                                                                                                                aGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCGCAGCAAACCAATATGGACGTCa                     >  2:798294/1‑150 (MQ=255)
                                                                                                                                   agttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAGATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTg                  <  2:1082847/148‑1 (MQ=255)
                                                                                                                                   aCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTg                  >  2:202098/1‑150 (MQ=255)
                                                                                                                                   aCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTg                  >  2:1218542/1‑150 (MQ=255)
                                                                                                                                    cTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGc                 >  2:765223/1‑150 (MQ=255)
                                                                                                                                      tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCca               >  1:1283835/1‑150 (MQ=255)
                                                                                                                                        tAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaac             <  1:2030089/150‑1 (MQ=255)
                                                                                                                                        tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAGTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAGCCAATATGGTCGTCTTTGCcagc             >  2:1889812/1‑148 (MQ=255)
                                                                                                                                          tgCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGAGATATGCTCTATGTCACTAAGCCACTACAGGACATTTTATGATCAGTTAATTATGGGTCGCCACATCGGGCCTTGACGCCCAGCAGCCCAATATGGACGTCATTGCcaacac           >  1:345663/2‑149 (MQ=255)
                                                                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa           >  2:1873946/1‑150 (MQ=255)
                                                                                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc         >  2:463039/1‑150 (MQ=255)
                                                                                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc         >  2:1823464/1‑150 (MQ=255)
                                                                                                                                            ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCATTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc         >  2:281603/1‑150 (MQ=255)
                                                                                                                                               gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg      <  2:1218461/150‑1 (MQ=255)
                                                                                                                                                   aGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTATGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATCGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGCACGTCATTGCCAACACCCTGGCaaa  >  2:343294/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAA  >  NC_000913/1134515‑1134811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: