Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,302,827 G→A 5.0% intergenic (+14/‑72) oppA → / → oppB oligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,302,8270GA 5.0% 363.6 / 3.9 117intergenic (+14/‑72)oppA/oppBoligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (63/48);  new base A (4/2);  total (67/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

ATAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATG  >  NC_000913/1302680‑1302976
                                                                                                                                                   |                                                                                                                                                     
aTAAGTATTCGGCCATTGTTGCTGTTTATTACTACGAGAATGCGCGTCTGTTGAAACCGTGGGTTGGTGGCTATACCGGCATAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGc                                                                                                                                                     <  1:123213/150‑1 (MQ=255)
aTAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGc                                                                                                                                                     <  2:1615480/150‑1 (MQ=255)
   aGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg                                                                                                                                                  >  1:1613286/1‑150 (MQ=255)
   aGGATTCGGCCAATGTTACTGTTTATTACTACGAGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATAACCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAgg                                                                                                                                                  <  2:1043907/150‑1 (MQ=255)
    ggATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGa                                                                                                                                                 >  1:427397/1‑150 (MQ=255)
     gATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGGGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAg                                                                                                                                                <  2:545135/150‑1 (MQ=255)
      aTTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCTCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAgt                                                                                                                                               >  1:124640/1‑150 (MQ=255)
         cGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTc                                                                                                                                            >  2:1639243/1‑150 (MQ=255)
            ccATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTg                                                                                                                                         >  1:2027012/1‑150 (MQ=255)
             cATTGGACCTGTCTACTACTCCGGGAATGCGCGTCTGGTGAACCCGTGGGTTGGTGGCTATACCGGCAAAGAGCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAATCACTAATGGCAATACGTGGGGCAGGAGGGTCCTGc                                                                                                                                        <  2:94827/150‑1 (MQ=255)
                 gTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTccc                                                                                                                                    >  2:198647/1‑149 (MQ=255)
                   tCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGCCCCACg                                                                                                                                  <  1:754213/150‑1 (MQ=255)
                    ccTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACgg                                                                                                                                 >  1:741688/1‑150 (MQ=255)
                    ccTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACgg                                                                                                                                 <  2:1755642/150‑1 (MQ=255)
                      tttttatttaTAAGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATCCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGgtg                                                                                                                               <  2:307143/148‑1 (MQ=255)
                       gTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTATTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGgtgt                                                                                                                              <  1:722189/150‑1 (MQ=255)
                         ttattaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCt                                                                                                                            >  1:784035/1‑150 (MQ=255)
                          tattaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTg                                                                                                                           <  1:1684662/150‑1 (MQ=255)
                          tattaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGGGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTg                                                                                                                           <  1:147865/150‑1 (MQ=255)
                              actacGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAttt                                                                                                                       <  1:482110/150‑1 (MQ=255)
                               ctacGTGACTGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt                                                                                                                      <  2:1547946/150‑1 (MQ=255)
                               ctacGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt                                                                                                                      >  2:972826/1‑150 (MQ=255)
                               ctacGTGAATGCGCGTCTGGTGAAACCGTGGGGTGGTGGCTATACCGGCAAAGATCCGCGGGATAATACCTATCCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt                                                                                                                      <  1:156932/150‑1 (MQ=255)
                                 acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTa                                                                                                                    >  1:2040455/1‑150 (MQ=255)
                                 acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTa                                                                                                                    >  2:1227111/1‑150 (MQ=255)
                                  cGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTAt                                                                                                                   >  2:1518495/1‑150 (MQ=255)
                                  cGTGAATGCGAGTCTGGTGAAACCGTGGGTTGGTGGCTATCCCGGCAAAGATCCGCTGGATAATACCTATACCCGGATTATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAGTTTTAt                                                                                                                   >  1:298524/1‑150 (MQ=255)
                                   gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATc                                                                                                                  >  1:575266/1‑150 (MQ=255)
                                   gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATc                                                                                                                  <  2:83059/150‑1 (MQ=255)
                                               cTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAg                                                                                                      >  2:1987668/1‑150 (MQ=255)
                                                 ggTGAAACCGTGGGGTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCAGTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGc                                                                                                    <  1:8138/150‑1 (MQ=255)
                                                   tGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCTCTGGATAATACATATACCCGGAATATGTAGATTGTGAAGCACTAATGGCAATACGTGGGGCATGAGTGTACTGCTCCACGGTGTCTGATTTTTATCGAATTACAGAAGGcaa                                                                                                  >  1:722955/1‑149 (MQ=255)
                                                       aCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGc                                                                                              >  2:1342150/1‑150 (MQ=255)
                                                       aCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGc                                                                                              >  1:1754243/1‑150 (MQ=255)
                                                        ccGGGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGcc                                                                                             <  1:936179/150‑1 (MQ=255)
                                                                tggtggCTATACCGGCAAAGATCCTCTTGATAATTCCTATACCCGGAAGATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTa                                                                                     <  1:334269/150‑1 (MQ=255)
                                                                 ggtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATCTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAg                                                                                    >  2:321145/1‑150 (MQ=255)
                                                                  gtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAgg                                                                                   >  2:1129045/1‑150 (MQ=255)
                                                                  gtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAgg                                                                                   <  2:1754518/150‑1 (MQ=255)
                                                                   tggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGGACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAggg                                                                                  <  1:1672176/150‑1 (MQ=255)
                                                                    ggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGc                                                                                 <  2:1061894/150‑1 (MQ=255)
                                                                     gCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTTCAGAAGGCACAGGCCAGAAGGTAGGGCa                                                                                >  2:972752/1‑150 (MQ=255)
                                                                        ataCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATg                                                                             <  2:1914291/150‑1 (MQ=255)
                                                                         taCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGt                                                                            >  2:1569445/1‑150 (MQ=255)
                                                                          aCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGtt                                                                           >  1:1989643/1‑150 (MQ=255)
                                                                           ccGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTa                                                                          >  2:892981/1‑150 (MQ=255)
                                                                           ccGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTa                                                                          >  2:1664941/1‑150 (MQ=255)
                                                                             ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                        >  1:1129986/1‑150 (MQ=255)
                                                                             ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                        >  1:2751/1‑150 (MQ=255)
                                                                             ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                        >  2:1384427/1‑150 (MQ=255)
                                                                                   gATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTAt                                                                  >  1:282827/1‑150 (MQ=255)
                                                                                    aTCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTAtt                                                                 <  2:400751/150‑1 (MQ=255)
                                                                                     tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCTCGGTGTCTGATTTTTATCGCATTACAGACGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc                                                                <  1:984031/150‑1 (MQ=255)
                                                                                     tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc                                                                <  1:1227112/150‑1 (MQ=255)
                                                                                      ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                               >  1:775121/1‑150 (MQ=255)
                                                                                      ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                               >  2:937793/1‑150 (MQ=255)
                                                                                      ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                               >  2:670740/1‑150 (MQ=255)
                                                                                      ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                               <  1:431760/150‑1 (MQ=255)
                                                                                          tGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGt                                                           >  2:1958366/1‑150 (MQ=255)
                                                                                                ataCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGt                                                     >  1:1870317/1‑150 (MQ=255)
                                                                                                 taCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTc                                                    >  1:372468/1‑150 (MQ=255)
                                                                                                 taCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTc                                                    <  1:1762719/150‑1 (MQ=255)
                                                                                                     tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGa                                                >  1:467923/1‑150 (MQ=255)
                                                                                                      ataCCCGGAATATGTACAGTGTGAAGCACTAATGGCAATACGTGGGGAAGTAGTGTCCTTCACCACGGTGGCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTATGTAAATGTTAAAATTTATTATACGTATCTGTcagaaa                                               >  2:295322/1‑145 (MQ=255)
                                                                                                       taCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAg                                              <  1:694010/150‑1 (MQ=255)
                                                                                                       taCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAg                                              <  1:1993370/150‑1 (MQ=255)
                                                                                                        aCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGc                                             <  2:1391307/150‑1 (MQ=255)
                                                                                                          ccGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGa                                           >  2:1335086/1‑150 (MQ=255)
                                                                                                           cGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCggt                                          >  1:1062974/1‑148 (MQ=255)
                                                                                                              aaTATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTcc                                       >  2:1770520/1‑150 (MQ=255)
                                                                                                                tatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGa                                     <  1:737082/150‑1 (MQ=255)
                                                                                                                tatGAACCTTGTCACGCACTAATGGCAATACCTCGGGCAGGCGTGGCCTGCTCCACGGTGTCTGATTTTAATCGCATTACAGAAGTCACAGGCCAGACGGTAGGGCAATGTTAAAAGTTATTCTACGTCCCTCTCTGGAACCGACTCCGa                                     <  2:83796/150‑1 (MQ=255)
                                                                                                                 atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGAc                                    <  1:1519297/150‑1 (MQ=255)
                                                                                                                    tACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCt                                 >  2:1953499/1‑150 (MQ=255)
                                                                                                                     aCATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCta                                >  2:473759/1‑150 (MQ=255)
                                                                                                                     aCATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTAAAAATTTATTCTATGTCGCTGTCTGGAAGCGATTCCGACGCta                                >  1:1321129/1‑150 (MQ=255)
                                                                                                                      cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCtat                               >  2:562677/1‑150 (MQ=255)
                                                                                                                          gtgAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCtatttat                           <  2:2040454/150‑1 (MQ=255)
                                                                                                                            gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGATGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACTTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                         <  2:1764674/150‑1 (MQ=255)
                                                                                                                            gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAATGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                         >  2:1189879/1‑150 (MQ=255)
                                                                                                                            gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                         >  1:723692/1‑150 (MQ=255)
                                                                                                                            gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                         >  2:34441/1‑150 (MQ=255)
                                                                                                                             aaGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCCCGGTGTCTGATTTTTATCGCATTACAGAAGGCACCGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCTCTGTCTGTAAGCGATTCAGACGCtatttattat                        >  2:1441211/1‑148 (MQ=255)
                                                                                                                             aaGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCt                        >  2:907591/1‑150 (MQ=255)
                                                                                                                               gCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCtta                      >  1:1978639/1‑150 (MQ=255)
                                                                                                                                cACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGAAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCttat                     <  2:426533/150‑1 (MQ=255)
                                                                                                                                 aCTAATGGCAATAGGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGTTCAATGTTAAAATTTATTCTACTTCGCTGTTTGGAAGCGATTACGACGCTATTTATTCttatt                    <  1:1205976/150‑1 (MQ=255)
                                                                                                                                 aCTAATGGCAATACGTGGGGCAGGAGTGTCCTGCGCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGTCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCttatt                    <  2:2027011/150‑1 (MQ=255)
                                                                                                                                    aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCCCCGGCCAGAAGGTTGGGCAAGGTTAAAATTTATACACCGACGCTGTATGGAAGCGATACCGACGCTATTTAAtattataagt                 >  2:198077/1‑146 (MQ=255)
                                                                                                                                    aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACt                 <  1:434374/150‑1 (MQ=255)
                                                                                                                                    aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACt                 >  2:1384975/1‑150 (MQ=255)
                                                                                                                                       ggCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTAtt              >  2:643365/1‑150 (MQ=255)
                                                                                                                                         cAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTc            <  2:1087572/150‑1 (MQ=255)
                                                                                                                                          aaTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCg           <  1:972827/150‑1 (MQ=255)
                                                                                                                                           caccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAACATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGt          >  1:406830/4‑150 (MQ=255)
                                                                                                                                           aTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGt          <  1:1619627/150‑1 (MQ=255)
                                                                                                                                           aTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGt          <  2:741687/150‑1 (MQ=255)
                                                                                                                                             ccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTc        >  2:1165954/2‑150 (MQ=255)
                                                                                                                                             ccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTc        >  1:899599/2‑150 (MQ=255)
                                                                                                                                                   ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatg  >  1:179537/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATAAGGATTCGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATG  >  NC_000913/1302680‑1302976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: