Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 125,939 A→C 5.9% D82A (GAC→GCC)  aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913125,9390AC 5.9% 357.6 / 4.2 126D82A (GAC→GCC) aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base A (55/62);  new base C (6/3);  total (61/65)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAG  >  NC_000913/125793‑126085
                                                                                                                                                  |                                                                                                                                                  
gCTGATCACCGTAGAAGTCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGc                                                                                                                                                 <  1:1173851/150‑1 (MQ=255)
   gATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGc                                                                                                                                              >  2:7658/1‑150 (MQ=255)
     tCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAc                                                                                                                                            <  1:1876618/150‑1 (MQ=255)
      cACCGTAGAAGTCGACTAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGATATCAACGTCTCTGTTGGCGATAAAACCCATACCGGCGCACTGATTCTGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAcc                                                                                                                                           <  1:34948/150‑1 (MQ=255)
      cACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGACATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAcc                                                                                                                                           <  2:497186/150‑1 (MQ=255)
         cGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCTATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCGTCTGCTGCAccgtc                                                                                                                                        <  2:594891/150‑4 (MQ=255)
         cGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCTGACGCTGCACCTGc                                                                                                                                        <  2:975851/150‑1 (MQ=255)
         cGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCGTCTGCTGCAccgtc                                                                                                                                        <  1:1675697/150‑4 (MQ=255)
          gTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCt                                                                                                                                       >  1:1315819/1‑150 (MQ=255)
           tAGAAGGCGACAAAGCCTCTAGGGAAGTTCCGTCTCCGCAGGCGGGTAGCTTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCGTCTGCTGCAccgtcgg                                                                                                                                      <  1:1876201/150‑6 (MQ=255)
            aGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCa                                                                                                                                     <  2:825422/150‑1 (MQ=255)
             gAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAATGGCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAg                                                                                                                                    <  1:1152425/150‑1 (MQ=255)
               aGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCTTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGAACGGCGCACTGATTATGATTTTCGATTACGCCGACGGTGCTGCTGACGCTGCACCTGCTCAGGc                                                                                                                                  >  2:339203/1‑150 (MQ=255)
                  cGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATTAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCaga                                                                                                                               >  2:1236435/1‑150 (MQ=255)
                  cGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCGCAGGCaga                                                                                                                               >  1:1272831/1‑150 (MQ=255)
                  cGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTAGCGTTAAAGAGATCAAAGTGTAGGTTGGGGAGAAACGGGCGACCGGCGCGCTGATTATGGTTTTCGATTGCGCCGCAGGTGCAGCAGCGGCTGCTCCTGCGCCGGCaga                                                                                                                               >  2:1180024/1‑150 (MQ=255)
                  cGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGGATCGTTAAAGAGATCAAAGTCTCGGTGGGGGAGAAAAACGCGACCGGCGCGCTGATTATGATATTCGAATCCGCCGCAGATGCAGCCGCCGCAGCACCTGCGCAGGCaga                                                                                                                               >  2:281978/1‑150 (MQ=255)
                   gACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGAGCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaa                                                                                                                              <  2:1469100/150‑1 (MQ=255)
                    aCAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaag                                                                                                                             <  2:1638628/150‑1 (MQ=255)
                     cAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGAAGGAGCAGCAGACGCTGCACCTGCTCAGGCacaaga                                                                                                                            >  1:1903579/1‑150 (MQ=255)
                      aaaGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaagag                                                                                                                           <  2:1707462/150‑1 (MQ=255)
                      aaaGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaagag                                                                                                                           >  2:2015442/1‑150 (MQ=255)
                              tATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGaagaaaga                                                                                                                   <  1:1716484/150‑1 (MQ=255)
                               aTGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAagaaagaa                                                                                                                  <  2:715299/150‑1 (MQ=255)
                                tGGAAGTGCCGTCTCCGCAGGCGGGTATCGTAAAAGAGATCAAAGTCTCTTTTGGCGATAAAACCCAGACCGGCGCACTGATTAGGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAaagaag                                                                                                                 <  2:401538/150‑1 (MQ=255)
                                 ggAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGATCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAagc                                                                                                                <  1:1296976/150‑1 (MQ=255)
                                  gAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAgcagca                                                                                                               >  1:245731/1‑150 (MQ=255)
                                  gAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAagca                                                                                                               >  1:470947/1‑150 (MQ=255)
                                       tCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTcc                                                                                                          <  1:585480/150‑1 (MQ=255)
                                        ccGTCTCCGCAGGAGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCg                                                                                                         <  2:513891/150‑1 (MQ=255)
                                         cGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTTGAGATAAAACCCAGACCGGCGCACTGATTAGGATTTTAGATTACGCCGACAGTGCAGCAGCCGCTGCACCTGATAAGGCAGAATCGAACAAATAAGAAGCtactg                                                                                                        >  2:339520/1‑146 (MQ=255)
                                         cGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCgg                                                                                                        >  1:1678328/1‑150 (MQ=255)
                                         cGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGATGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCgg                                                                                                        <  1:310022/150‑1 (MQ=255)
                                          gTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgc                                                                                                       >  1:1697813/1‑150 (MQ=255)
                                            ctcCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcag                                                                                                     >  1:249119/1‑150 (MQ=255)
                                             gccGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCAGTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagc                                                                                                    <  2:1337167/149‑1 (MQ=255)
                                              ccGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagca                                                                                                   <  1:1114609/150‑1 (MQ=255)
                                              ccGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagca                                                                                                   >  2:1577275/1‑150 (MQ=255)
                                              ccGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagca                                                                                                   <  2:1483483/150‑1 (MQ=255)
                                               cGCAGGCGGGTATCGTTAACGAGATCAAAGTCTCTGCTGGCTATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagcag                                                                                                  <  2:694092/150‑1 (MQ=255)
                                                gCAGGCGGTTAGCGGTAAAGACATCATAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATATCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagcagc                                                                                                 <  2:338994/150‑1 (MQ=255)
                                                gCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGAAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagcagc                                                                                                 >  1:151010/1‑150 (MQ=255)
                                                      gggTATCTTTACAGAGATCAAAGCCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATACCGCCGACGGGGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGc                                                                                           <  1:1757257/150‑1 (MQ=255)
                                                       ggaaTCGTTAAAGAGATCAAAGTCTATGTTGGCGATAAAACCCAGCCCGGCGCACTGATTATGATTTTCGATACAGCCGACGGTGCAGCAGACGCAGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCAGGCCGAAGCACCAGCg                                                                                          >  1:166131/4‑150 (MQ=255)
                                                            cGTTAAAGAGATCAAAGTCTTTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGCCGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCATCAGCAACAGCGGCTgc                                                                                     >  2:1195634/1‑150 (MQ=255)
                                                             gTTAAAGAGATCAACGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTgcg                                                                                    <  2:1094842/150‑1 (MQ=255)
                                                             gTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTgcg                                                                                    >  2:1950609/1‑150 (MQ=255)
                                                               tAAAGAGATCAAAGGCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTgcggc                                                                                  <  1:1016195/150‑1 (MQ=255)
                                                                 aaGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGcggcgg                                                                                <  1:1270442/150‑1 (MQ=255)
                                                                  acagctCAAAGTCTCTGTTGGCGATAAACCCCAGACCGGCGCACTGATACTGATTTTCGCTTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCggcggc                                                                               <  1:82179/145‑1 (MQ=255)
                                                                   gagcTCAAAGTCTCCGTTGGCGATCACCCCCAGACCGGCGCACTGCTGCTGATTTTCGATTCCGCCGCCGGGGCCGCCGACGCTGCACCGGCTCCGGCAGCCGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCa                                                                              <  1:1091867/150‑1 (MQ=255)
                                                                   gagaTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCa                                                                              <  2:108817/150‑1 (MQ=255)
                                                                     gaTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCCCCAGCGGCTGCGGCGGCaaa                                                                            >  1:1034180/1‑150 (MQ=255)
                                                                     gaTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCCACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAATCAGCTCCGGCAGCGGCACCTGCGCCAGCGGCAGaaaa                                                                            >  1:1205685/1‑150 (MQ=255)
                                                                      aTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCaaaa                                                                           >  1:1716488/1‑150 (MQ=255)
                                                                            gTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGtt                                                                     <  1:329176/150‑1 (MQ=255)
                                                                              ctctGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTaa                                                                   <  2:651166/150‑1 (MQ=255)
                                                                                ctGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACg                                                                 <  2:665724/150‑1 (MQ=255)
                                                                                ctGTTGCCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACg                                                                 <  1:610999/150‑1 (MQ=255)
                                                                                 tGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCGGCGGCTGCGGCGGCAAAAGACGTTAACGt                                                                >  2:1628888/1‑150 (MQ=255)
                                                                                     ggCGATAAACCCCAGACCGGCGCACTGATTCTGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCg                                                            <  1:212364/150‑1 (MQ=255)
                                                                                       cGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGa                                                          >  2:94276/1‑150 (MQ=255)
                                                                                            aaaCCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTTCTCAGGCAGAATAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTTACGTTCCCGATATCg                                                     >  2:721457/1‑150 (MQ=255)
                                                                                            aaaCCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCg                                                     >  1:94997/1‑150 (MQ=255)
                                                                                             aaCCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGAAGTTAACGTTCCGGATATctg                                                    >  2:938786/1‑148 (MQ=255)
                                                                                              aCCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGc                                                   <  1:1366674/150‑1 (MQ=255)
                                                                                              aCCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGc                                                   >  1:1838765/1‑150 (MQ=255)
                                                                                               cccAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCa                                                  <  1:993617/150‑1 (MQ=255)
                                                                                                  aGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcg                                               <  2:1315818/150‑1 (MQ=255)
                                                                                                     ccGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGcgacg                                            >  1:733042/1‑150 (MQ=255)
                                                                                                        gcgcACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAg                                         <  1:7658/150‑1 (MQ=255)
                                                                                                          gcACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCCGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGtt                                       >  2:1665638/1‑150 (MQ=255)
                                                                                                            aCTGATTATGATTTTCGATTCCGCCGCCGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGa                                     <  2:1688209/150‑1 (MQ=255)
                                                                                                             cTGATTAAGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCCCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGaa                                    >  2:1218349/1‑150 (MQ=255)
                                                                                                              tGATTATGTTTTTCTATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAg                                   <  2:1272830/150‑1 (MQ=255)
                                                                                                               gATTATGATTTTCGAATCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCCGCACCAGCGGCTGCGGCGGCAAAAGACGTTTACGATCCGGATAACGGCAGCGACGAAGTAGAAGt                                  >  1:372972/1‑150 (MQ=255)
                                                                                                                aTTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTg                                 <  1:600937/150‑1 (MQ=255)
                                                                                                                 ttATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTgc                                >  2:1405369/1‑149 (MQ=255)
                                                                                                                 ttATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGa                                >  1:1979602/1‑150 (MQ=255)
                                                                                                                  tATGATTTTCGATTCCGACGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAc                               <  1:224561/150‑1 (MQ=255)
                                                                                                                   aTGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAcc                              <  1:832681/150‑1 (MQ=255)
                                                                                                                   aTGATTTTCGAGTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCGGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGAcc                              <  1:825467/150‑1 (MQ=255)
                                                                                                                    tGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCg                             >  1:1672196/1‑150 (MQ=255)
                                                                                                                         ttCGATTCCGACGACGGTGCATCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCAGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATc                        <  2:1442695/150‑1 (MQ=255)
                                                                                                                           cGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCAGCAGCGGATGCGGCGGCAAAAGTCGTTTACGTTCCGGATATCGGCAGCGACGAAGTGGAAGTGCCCGAAATCCt                      >  2:1548454/1‑150 (MQ=255)
                                                                                                                           cGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCt                      <  1:145739/150‑1 (MQ=255)
                                                                                                                           cGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAAAGTTCCGGATATCGGCGGAGACGAAGTTGAAGTGACCGatcggaa                      >  2:342904/1‑144 (MQ=255)
                                                                                                                            gATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTg                     >  2:1038678/1‑150 (MQ=255)
                                                                                                                            gATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTg                     >  1:1631221/1‑150 (MQ=255)
                                                                                                                            gATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGAGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTg                     <  2:1319029/150‑1 (MQ=255)
                                                                                                                             aTTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTgg                    <  1:1571773/150‑1 (MQ=255)
                                                                                                                              ttCCGCCTACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACTTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGt                   <  1:1041338/150‑1 (MQ=255)
                                                                                                                              ttCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCTGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGt                   <  2:698297/150‑1 (MQ=255)
                                                                                                                              ttCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGt                   <  1:1397397/150‑1 (MQ=255)
                                                                                                                              ttCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGt                   >  1:1364297/1‑150 (MQ=255)
                                                                                                                                ccgccgACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGa                 >  1:632175/1‑150 (MQ=255)
                                                                                                                                      aCGGTGCAGCCGACGCTGCACCTGCGCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTg           <  1:1936137/150‑1 (MQ=255)
                                                                                                                                           gcagcaGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAATATGAAGTGACAGAAATCCTGGTGAAAGTTGTCGaa      >  2:193771/1‑149 (MQ=255)
                                                                                                                                             agcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATaa    >  1:1959779/1‑150 (MQ=255)
                                                                                                                                               cagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAg  <  1:2015443/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAG  >  NC_000913/125793‑126085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: