Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,302,836 G→C 5.1% intergenic (+23/‑63) oppA → / → oppB oligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,302,8360GC 5.1% 391.1 / 9.9 117intergenic (+23/‑63)oppA/oppBoligopeptide ABC transporter periplasmic binding protein/oligopeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (65/46);  new base C (4/2);  total (69/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 119 total aligned reads displayed.

CGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTC  >  NC_000913/1302689‑1302985
                                                                                                                                                   |                                                                                                                                                     
cGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTc                                                                                                                                                     >  2:1639243/1‑150 (MQ=255)
   ccATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTg                                                                                                                                                  >  1:2027012/1‑150 (MQ=255)
    cATTGGACCTGTCTACTACTCCGGGAATGCGCGTCTGGTGAACCCGTGGGTTGGTGGCTATACCGGCAAAGAGCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAATCACTAATGGCAATACGTGGGGCAGGAGGGTCCTGc                                                                                                                                                 <  2:94827/150‑1 (MQ=255)
        gTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTccc                                                                                                                                             >  2:198647/1‑149 (MQ=255)
         ttCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTTGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGCCCCAc                                                                                                                                            <  2:590807/150‑1 (MQ=255)
          tCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGAGCAGGACACTCCTGCCCCACg                                                                                                                                           <  1:754213/150‑1 (MQ=255)
           ccTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACgg                                                                                                                                          >  1:741688/1‑150 (MQ=255)
           ccTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACgg                                                                                                                                          <  2:1755642/150‑1 (MQ=255)
             tttttatttaTAAGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATCCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGgtg                                                                                                                                        <  2:307143/148‑1 (MQ=255)
              gTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTATTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGgtgt                                                                                                                                       <  1:722189/150‑1 (MQ=255)
                ttattaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCt                                                                                                                                     >  1:784035/1‑150 (MQ=255)
                 tattaCTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTg                                                                                                                                    <  1:1684662/150‑1 (MQ=255)
                      ctacGTGACTGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt                                                                                                                               <  2:1547946/150‑1 (MQ=255)
                      ctacGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt                                                                                                                               >  2:972826/1‑150 (MQ=255)
                      ctacGTGAATGCGCGTCTGGTGAAACCGTGGGGTGGTGGCTATACCGGCAAAGATCCGCGGGATAATACCTATCCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAtttt                                                                                                                               <  1:156932/150‑1 (MQ=255)
                        acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTa                                                                                                                             >  2:1227111/1‑150 (MQ=255)
                        acGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTa                                                                                                                             >  1:2040455/1‑150 (MQ=255)
                         cGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTAt                                                                                                                            >  2:1518495/1‑150 (MQ=255)
                         cGTGAATGCGAGTCTGGTGAAACCGTGGGTTGGTGGCTATCCCGGCAAAGATCCGCTGGATAATACCTATACCCGGATTATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGAGTTTTAt                                                                                                                            >  1:298524/1‑150 (MQ=255)
                          gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATc                                                                                                                           >  1:575266/1‑150 (MQ=255)
                          gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATc                                                                                                                           <  2:83059/150‑1 (MQ=255)
                          gTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGGGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATc                                                                                                                           <  2:1045363/150‑1 (MQ=255)
                              aTGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCAt                                                                                                                       <  2:1333316/150‑1 (MQ=255)
                                      cTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAg                                                                                                               >  2:1987668/1‑150 (MQ=255)
                                          tGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCTCTGGATAATACATATACCCGGAATATGTAGATTGTGAAGCACTAATGGCAATACGTGGGGCATGAGTGTACTGCTCCACGGTGTCTGATTTTTATCGAATTACAGAAGGcaa                                                                                                           >  1:722955/1‑149 (MQ=255)
                                              aCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGc                                                                                                       >  1:1754243/1‑150 (MQ=255)
                                              aCCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGc                                                                                                       >  2:1342150/1‑150 (MQ=255)
                                               ccGGGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGcc                                                                                                      <  1:936179/150‑1 (MQ=255)
                                                    ggTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAg                                                                                                 >  1:1253126/1‑150 (MQ=255)
                                                       tggtggCTATACCGGCAAAGATCCTCTTGATAATTCCTATACCCGGAAGATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTa                                                                                              <  1:334269/150‑1 (MQ=255)
                                                        ggtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATCTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAg                                                                                             >  2:321145/1‑150 (MQ=255)
                                                         gtggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAgg                                                                                            >  2:1129045/1‑150 (MQ=255)
                                                           ggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGc                                                                                          >  1:385920/1‑150 (MQ=255)
                                                           ggCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGc                                                                                          <  2:1061894/150‑1 (MQ=255)
                                                            gCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTTCAGAAGGCACAGGCCAGAAGGTAGGGCa                                                                                         >  2:972752/1‑150 (MQ=255)
                                                               ataCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATg                                                                                      <  2:1914291/150‑1 (MQ=255)
                                                                taCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGt                                                                                     >  2:1569445/1‑150 (MQ=255)
                                                                 aCCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGtt                                                                                    >  1:1989643/1‑150 (MQ=255)
                                                                  ccGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTa                                                                                   >  2:1448401/1‑150 (MQ=255)
                                                                  ccGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTa                                                                                   >  2:892981/1‑150 (MQ=255)
                                                                  ccGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTa                                                                                   >  2:1664941/1‑150 (MQ=255)
                                                                    ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                                 >  2:1384427/1‑150 (MQ=255)
                                                                    ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                                 >  1:1129986/1‑150 (MQ=255)
                                                                    ggCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaa                                                                                 >  1:2751/1‑150 (MQ=255)
                                                                     gCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTaaaa                                                                                >  1:530916/1‑150 (MQ=255)
                                                                          gATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTAt                                                                           >  1:282827/1‑150 (MQ=255)
                                                                           aTCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTAtt                                                                          <  2:400751/150‑1 (MQ=255)
                                                                            tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCTCGGTGTCTGATTTTTATCGCATTACAGACGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc                                                                         <  1:984031/150‑1 (MQ=255)
                                                                            tCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTc                                                                         <  1:1227112/150‑1 (MQ=255)
                                                                             ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                                        >  1:775121/1‑150 (MQ=255)
                                                                             ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                                        >  2:937793/1‑150 (MQ=255)
                                                                             ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                                        >  2:670740/1‑150 (MQ=255)
                                                                             ccGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCt                                                                        <  1:431760/150‑1 (MQ=255)
                                                                                 tGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGt                                                                    >  2:1958366/1‑150 (MQ=255)
                                                                                        taCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTc                                                             <  1:1762719/150‑1 (MQ=255)
                                                                                        taCCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTc                                                             >  1:372468/1‑150 (MQ=255)
                                                                                            tataCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGa                                                         >  1:467923/1‑150 (MQ=255)
                                                                                             ataCCCGGAATATGTACAGTGTGAAGCACTAATGGCAATACGTGGGGAAGTAGTGTCCTTCACCACGGTGGCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTATGTAAATGTTAAAATTTATTATACGTATCTGTcagaaa                                                        >  2:295322/1‑145 (MQ=255)
                                                                                              taCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAg                                                       <  1:1993370/150‑1 (MQ=255)
                                                                                              taCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAg                                                       <  1:694010/150‑1 (MQ=255)
                                                                                               aCCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGc                                                      <  2:1391307/150‑1 (MQ=255)
                                                                                                 ccGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGa                                                    >  2:1335086/1‑150 (MQ=255)
                                                                                                  cGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCggt                                                   >  1:1062974/1‑148 (MQ=255)
                                                                                                     aaTATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTcc                                                >  2:1770520/1‑150 (MQ=255)
                                                                                                       tatGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGa                                              <  1:737082/150‑1 (MQ=255)
                                                                                                       tatGAACCTTGTCACGCACTAATGGCAATACCTCGGGCAGGCGTGGCCTGCTCCACGGTGTCTGATTTTAATCGCATTACAGAAGTCACAGGCCAGACGGTAGGGCAATGTTAAAAGTTATTCTACGTCCCTCTCTGGAACCGACTCCGa                                              <  2:83796/150‑1 (MQ=255)
                                                                                                        atGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGAc                                             <  1:1519297/150‑1 (MQ=255)
                                                                                                          gTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGc                                           >  2:1656835/1‑150 (MQ=255)
                                                                                                            aCATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCta                                         >  2:473759/1‑150 (MQ=255)
                                                                                                            aCATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTAAAAATTTATTCTATGTCGCTGTCTGGAAGCGATTCCGACGCta                                         >  1:1321129/1‑150 (MQ=255)
                                                                                                             cATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCtat                                        >  2:562677/1‑150 (MQ=255)
                                                                                                              aTTCTGAAGCACTAATGGCAATACGTGGGGCCGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTTTCTGGAAGCGATTCCGACGCtatt                                       <  1:1839866/150‑1 (MQ=255)
                                                                                                                 gtgAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCtatttat                                    <  2:2040454/150‑1 (MQ=255)
                                                                                                                 gtgAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATAACAGAAGGCACAGACCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCtatttat                                    >  1:724513/1‑150 (MQ=255)
                                                                                                                   gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGATGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACTTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                                  <  2:1764674/150‑1 (MQ=255)
                                                                                                                   gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAATGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                                  >  2:1189879/1‑150 (MQ=255)
                                                                                                                   gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                                  >  2:1944652/1‑150 (MQ=255)
                                                                                                                   gAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTc                                  >  2:34441/1‑150 (MQ=255)
                                                                                                                    aaGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCCCGGTGTCTGATTTTTATCGCATTACAGAAGGCACCGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCTCTGTCTGTAAGCGATTCAGACGCtatttattat                                 >  2:1441211/1‑148 (MQ=255)
                                                                                                                    aaGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCt                                 >  2:907591/1‑150 (MQ=255)
                                                                                                                      gCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCtta                               >  1:1978639/1‑150 (MQ=255)
                                                                                                                       cACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGAAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCttat                              <  2:426533/150‑1 (MQ=255)
                                                                                                                        actactGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCttatt                             <  1:81535/150‑1 (MQ=255)
                                                                                                                        aCTAATGGCAATAGGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGTTCAATGTTAAAATTTATTCTACTTCGCTGTTTGGAAGCGATTACGACGCTATTTATTCttatt                             <  1:1205976/150‑1 (MQ=255)
                                                                                                                          tAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTAc                           <  1:1639244/150‑1 (MQ=255)
                                                                                                                           aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCCCCGGCCAGAAGGTTGGGCAAGGTTAAAATTTATACACCGACGCTGTATGGAAGCGATACCGACGCTATTTAAtattataagt                          >  2:198077/1‑146 (MQ=255)
                                                                                                                           aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACt                          >  2:1384975/1‑150 (MQ=255)
                                                                                                                           aaTGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACt                          <  1:434374/150‑1 (MQ=255)
                                                                                                                                cAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTc                     <  2:1087572/150‑1 (MQ=255)
                                                                                                                                 aaTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCg                    <  1:972827/150‑1 (MQ=255)
                                                                                                                                  caccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAACATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGt                   >  1:406830/4‑150 (MQ=255)
                                                                                                                                  aTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGt                   <  1:1619627/150‑1 (MQ=255)
                                                                                                                                  aTACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGt                   <  2:741687/150‑1 (MQ=255)
                                                                                                                                    ccGTGGAGCAGGACACTCCTGCCCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTc                 >  1:899599/2‑150 (MQ=255)
                                                                                                                                    aCGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGttg                 >  2:2023177/1‑149 (MQ=255)
                                                                                                                                          ggCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatg           >  1:179537/1‑150 (MQ=255)
                                                                                                                                           gCAGGAGTGTCCTGCGCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatga          <  2:1116147/150‑1 (MQ=255)
                                                                                                                                            cAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTatgat         <  1:1987669/150‑1 (MQ=255)
                                                                                                                                               gAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATgcg      >  2:223496/1‑150 (MQ=255)
                                                                                                                                                aGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATgcgc     >  1:1650163/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGCCATTGTTCCTGTTTATTACTACGTGAATGCGCGTCTGGTGAAACCGTGGGTTGGTGGCTATACCGGCAAAGATCCGCTGGATAATACCTATACCCGGAATATGTACATTGTGAAGCACTAATGGCAATACGTGGGGCAGGAGTGTCCTGCTCCACGGTGTCTGATTTTTATCGCATTACAGAAGGCACAGGCCAGAAGGTAGGGCAATGTTAAAATTTATTCTACGTCGCTGTCTGGAAGCGATTCCGACGCTATTTATTCTTATTACTATTTCGTTCTTTATGATGCGCCTC  >  NC_000913/1302689‑1302985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: