Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,306,788 T→C 5.0% intergenic (+20/+33) oppF → / ← yciU oligopeptide ABC transporter ATPase/UPF0263 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,306,7880TC 5.0% 362.6 / 6.8 121intergenic (+20/+33)oppF/yciUoligopeptide ABC transporter ATPase/UPF0263 family protein
Reads supporting (aligned to +/- strand):  ref base T (58/56);  new base C (4/2);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

Only 100 of 125 total aligned reads displayed.

AACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTC  >  NC_000913/1306640‑1306937
                                                                                                                                                    |                                                                                                                                                      
 aCCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTg                                                                                                                                                      <  1:247420/150‑1 (MQ=255)
   ccGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                    <  1:1968580/150‑1 (MQ=255)
   ccGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                    >  2:1739110/1‑150 (MQ=255)
        ttCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGccc                                                                                                                                               <  1:1567738/150‑1 (MQ=255)
          ccGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtt                                                                                                                                             <  1:1157857/150‑1 (MQ=255)
              ttGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTc                                                                                                                                         <  1:1545755/150‑1 (MQ=255)
               tgtgTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCa                                                                                                                                        <  1:1070038/150‑1 (MQ=255)
               tgtgTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCa                                                                                                                                        >  2:711559/1‑150 (MQ=255)
                 tgtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAACTGTCAGCCCttattgttt                                                                                                                                      <  2:445521/150‑5 (MQ=255)
                  gtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGc                                                                                                                                     >  2:2031247/1‑150 (MQ=255)
                   ttatCCGTACCCGTTGCCCGCTTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCt                                                                                                                                    <  1:1286232/147‑1 (MQ=255)
                        cGTACCCCTTGCCCGATTGCCGGTACAGAGTGCGCCAAAACACGTCCTGTTCGGGTGGGGAGTTTCAGACACTCCGTTTCTTGCCTGCAAGTCGATCCGCGTTTAAATCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTATAAg                                                                                                                               >  1:1099175/1‑150 (MQ=255)
                         gTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGGTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGc                                                                                                                              <  1:794959/150‑1 (MQ=255)
                            cccGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTGTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGat                                                                                                                           >  1:725236/1‑150 (MQ=255)
                            cccGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTGCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGCGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGat                                                                                                                           <  1:108721/150‑1 (MQ=255)
                              cGTTGCCCGATTGCCGGTCCAGAGTGCGACAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGGTTTATAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGatta                                                                                                                         >  2:152058/1‑150 (MQ=255)
                                  gCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattta                                                                                                                     >  1:1371268/1‑150 (MQ=255)
                                  gCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGAGCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCGTTTTCACGCTAACAGCGAttattta                                                                                                                     >  1:328587/1‑150 (MQ=255)
                                   cccGATTGCCGGTCCAGAGTGCGCCAAATCACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAGATCAATAAGGGCTGAGAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttattttt                                                                                                                    >  1:253509/1‑148 (MQ=255)
                                       aTTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAACGCGATTATTTATTccc                                                                                                                <  2:821400/150‑1 (MQ=255)
                                          gcgggTCCAGATGGCGCGAAAACACGTCCTGTTCTGGAGGGGAGTTGCAGACACTCCGTTGCTTGACTCAAAGTCGATCCGCTTTAAAAACAAAAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                             <  1:987797/147‑1 (MQ=255)
                                          gCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGCTCCGCTTTAAAAACAATACGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                             >  1:274681/1‑150 (MQ=255)
                                          gCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                             >  2:949344/1‑150 (MQ=255)
                                          gCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                             >  1:18613/1‑150 (MQ=255)
                                           ccGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCa                                                                                                            >  1:769087/1‑150 (MQ=255)
                                             ggTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAga                                                                                                          <  1:1670247/150‑1 (MQ=255)
                                              gTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAACAATGAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                        <  2:1077480/150‑1 (MQ=255)
                                               tCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatg                                                                                                        >  2:1404415/1‑150 (MQ=255)
                                                ccAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatga                                                                                                       >  1:1123268/1‑150 (MQ=255)
                                                ccAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGACGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGAGATTATTTATTCCCGCCAgatga                                                                                                       >  1:844542/1‑150 (MQ=255)
                                                   gagTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGATGTGAGCACTTTTTCCCGCTAAAAGCGATTATTTATACCCGCCAGAGGatat                                                                                                    >  2:256958/1‑150 (MQ=255)
                                                        cgcCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCGTGTTCACGCTAAAAGCGATTAGTTATTCCCGCCAGAGGAGAGGGCaa                                                                                               >  2:387995/1‑150 (MQ=255)
                                                        cgcCAAAACACGTCCTGTGCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                                                                               >  2:777104/1‑150 (MQ=255)
                                                          ccAAAACCCGTCCTGTTCTGGAGGTGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACACTAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                             <  1:1419311/150‑1 (MQ=255)
                                                          ccAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAg                                                                                             >  1:1947083/1‑150 (MQ=255)
                                                                cacGTCCTGTTCGGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTtgtg                                                                                       <  2:1301435/150‑1 (MQ=255)
                                                                     ccTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCtt                                                                                  >  2:214207/1‑150 (MQ=255)
                                                                     ccTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCtt                                                                                  >  1:567200/1‑150 (MQ=255)
                                                                      cTGTTCTGGAGGGGAGTGTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttt                                                                                 <  2:879835/150‑1 (MQ=255)
                                                                        gTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCttttt                                                                               <  2:1457006/150‑1 (MQ=255)
                                                                           cTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgc                                                                            <  1:2001921/150‑1 (MQ=255)
                                                                           cTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgc                                                                            <  2:1873341/150‑1 (MQ=255)
                                                                           cTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTcgc                                                                            >  1:873378/1‑150 (MQ=255)
                                                                             ttaGGGTAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAATGGATGACAGTTGTAAGACCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCgg                                                                          <  1:940294/148‑1 (MQ=255)
                                                                             ggAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGATGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCgg                                                                          >  1:1130362/1‑150 (MQ=255)
                                                                               tggggATGTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCa                                                                        <  1:905015/149‑1 (MQ=255)
                                                                                ggggAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCAt                                                                       >  2:728514/1‑150 (MQ=255)
                                                                                  ggAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATaa                                                                     >  1:1083420/1‑150 (MQ=255)
                                                                                  ggAGTTTCAGACACTCCGTTTCATGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGAGATTATTTATTCCCGCCAGATGATCGGGCAAAGTTTGTGATCTTTTTCGCGGCATaa                                                                     <  1:825729/150‑1 (MQ=255)
                                                                                     gTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAg                                                                  >  1:737086/1‑150 (MQ=255)
                                                                                     gTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAg                                                                  <  1:498259/150‑1 (MQ=255)
                                                                                       ttCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGaa                                                                >  1:1161848/1‑150 (MQ=255)
                                                                                         cAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATg                                                              >  2:1150298/1‑150 (MQ=255)
                                                                                             cacTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCTCCAGATGATATGGAAAAGTTTGTGATCTTTTTCTCGTCATAACATAATGCggg                                                          >  1:336279/1‑150 (MQ=255)
                                                                                             cacTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCggg                                                          <  2:1384708/150‑1 (MQ=255)
                                                                                             cacTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCggg                                                          <  2:18613/150‑1 (MQ=255)
                                                                                             cacTCCGTTTCTTGCCTGAAAGCCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCTGATGATTTGGCTAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCggg                                                          <  1:674290/150‑1 (MQ=255)
                                                                                              cctgcGTTTATTGCCTGAAAGTCGTTCCGCTTTTAAATGAATAATGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCGCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGTCATAACAGAATGCGGGc                                                         <  2:1713509/146‑1 (MQ=255)
                                                                                              acTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAATACAATAATGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTACCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGc                                                         <  2:1718774/150‑1 (MQ=255)
                                                                                               cTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCa                                                        <  1:26809/150‑1 (MQ=255)
                                                                                                tCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCaa                                                       <  1:1739111/150‑1 (MQ=255)
                                                                                                    tttCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAAc                                                   <  1:466854/150‑1 (MQ=255)
                                                                                                     ttCTTGCCTGAAAGTCGATCCGCTTTAAATGCAATAAGGGCTGACAGTTTTCAGCCATTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTGTTCGCGGCATAACAGAATGCGGGCAAAAACa                                                  <  2:1829831/150‑1 (MQ=255)
                                                                                                     ttCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGAAAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTACCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCTCGGCATAACAGAAGGCGGGCAAAACCa                                                  >  1:442044/1‑150 (MQ=255)
                                                                                                      tCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGCCATAACAGAATGCGGGCAAAAACAt                                                 <  2:2033158/150‑1 (MQ=255)
                                                                                                       cTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATc                                                >  2:422833/1‑150 (MQ=255)
                                                                                                        ttGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCg                                               >  1:1728776/1‑150 (MQ=255)
                                                                                                         tGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGt                                             >  2:1988681/1‑150 (MQ=255)
                                                                                                         tGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATATTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGt                                             >  2:1964755/1‑150 (MQ=255)
                                                                                                          gCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTAATTATTCCCGCCAGATGATATGGCAAAGATTCTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGtt                                            >  2:834786/1‑150 (MQ=255)
                                                                                                              gAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTGGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCCGATGATATGGCAAAGGTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAtt                                        <  1:1285162/150‑1 (MQ=255)
                                                                                                                aaGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTc                                      >  1:2043431/1‑150 (MQ=255)
                                                                                                                  gTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAc                                    <  2:1932765/150‑1 (MQ=255)
                                                                                                                  gTCGATCCGCTTTAAAAACAATAAGGGCGGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAc                                    <  1:1507236/150‑1 (MQ=255)
                                                                                                                    cGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTTATCTTTTTAGCGGCATAACAGATTGCGGGCAAAAACATCGTTGATTTCAccc                                  >  1:1300029/1‑149 (MQ=255)
                                                                                                                      aTCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATc                                <  1:1404416/150‑1 (MQ=255)
                                                                                                                       tCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCt                               >  2:1429857/1‑150 (MQ=255)
                                                                                                                         cGCTTTAAAAACAATAAGGGCTGACATTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTc                             <  1:1283987/150‑1 (MQ=255)
                                                                                                                         cGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTc                             >  2:1694778/1‑150 (MQ=255)
                                                                                                                          gCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATACCGGATTGCGGGAAAAACAAAGTTTGATTTCCCCATCtgaa                            >  1:1560053/1‑147 (MQ=255)
                                                                                                                           cTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCAc                           <  1:882826/150‑1 (MQ=255)
                                                                                                                             ttAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGATAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTg                         <  1:982445/150‑1 (MQ=255)
                                                                                                                               aaaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTc                       >  1:1897548/1‑150 (MQ=255)
                                                                                                                               aaaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTc                       >  1:7242/1‑150 (MQ=255)
                                                                                                                                aaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTcc                      >  1:1892348/1‑150 (MQ=255)
                                                                                                                                aaaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTcc                      <  2:1123267/150‑1 (MQ=255)
                                                                                                                                 aaaCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACAATCTTCACTGTCCg                     >  1:1939781/1‑150 (MQ=255)
                                                                                                                                  gtgaaaaaGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGc                    >  2:1308059/7‑150 (MQ=255)
                                                                                                                                   aCAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGcc                   <  2:1371267/150‑1 (MQ=255)
                                                                                                                                       tAAGGTATGACAGTTGTCATCCATTTTTCACGCTAAAAGCTATTATTTATTCCCTCCAGATGATATCGCAAATTTTGTGATCTTTTTCGCGGCATCTCAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAc               <  1:360873/150‑1 (MQ=255)
                                                                                                                                       tAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAc               <  1:1422404/150‑1 (MQ=255)
                                                                                                                                       tAAGGGCTGACAGTTGCCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGAc               <  1:1692491/150‑1 (MQ=255)
                                                                                                                                         aGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCa             >  2:1117420/1‑150 (MQ=255)
                                                                                                                                         aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCa             >  1:505292/1‑150 (MQ=255)
                                                                                                                                         aGGGCTGACAACTGTCAGCCCTTTTTCACGCTAAAAGCGATTAATTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGCATGCGGGCAAACACATCGTTGATTTCACCAACTTCACTGTCCGCCAGACCa             >  2:193950/1‑150 (MQ=255)
                                                                                                                                           ggCTGACAGTTGTCAGCCCTTTTTCACGCTAAACGCGATTATTTATACCCGCGAGATGATAAGGCAAAGTTTGCGATCTTTTTCGCGGCATTACAGAATGCGGGCAAAAACATCGTTTATATCACCATCTTCCCTGTCCGCCAGACCAAt           <  2:318050/150‑1 (MQ=255)
                                                                                                                                            gCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATc          <  1:1874755/150‑1 (MQ=255)
                                                                                                                                                 cAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATcaccac     <  2:1140356/150‑1 (MQ=255)
                                                                                                                                                    ttGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTc  <  2:513476/150‑1 (MQ=255)
                                                                                                                                                    ttGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTc  <  2:1670735/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
AACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTC  >  NC_000913/1306640‑1306937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: